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一种HBV研究的新动物模型-喜马拉雅旱獭β-actin基因的克隆及序列分析

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医学分子生物学杂志 2011年第2期8卷 95-99页

作者：冯雪梅尹莹李安意朱珍妮陶元清王忠东陆蒙吉杨东亮王宝菊华中科技大学同济医学院附属同济医院临床免疫研究室武汉市430030 华中科技大学同济医学院附属同济医院实验动物中心武汉市430030 青海省地方病预防控制所实验动物中心西宁市811602 德国Duisburg-Essen大学医学院病毒学研究所德国埃森45122

目的喜马拉雅旱獭对土拨鼠肝炎病毒（woodchuck hepatitis virus,WHV）高度易感,可作为HBV感染的新动物模型.本研究对喜马拉雅旱獭β肌动蛋白（β-actin）的部分cDNA序列进行了克隆和序列分析,为喜马拉雅旱獭在HBV感染研究中的应用奠... 详细信息

目的喜马拉雅旱獭对土拨鼠肝炎病毒（woodchuck hepatitis virus,WHV）高度易感,可作为HBV感染的新动物模型.本研究对喜马拉雅旱獭β肌动蛋白（β-actin）的部分cDNA序列进行了克隆和序列分析,为喜马拉雅旱獭在HBV感染研究中的应用奠定基础.方法根据Genbank的土拨鼠β-actin cDNA的序列设计特异性引物,提取旱獭脾组织总RNA作为模板,RT-PCR扩增旱獭β-actin cDNA序列;PCR产物纯化后连接至T载体（pMD18-T）,构建重组质粒pMD18-T-mhActin.对重组质粒进行PCR初筛及酶切鉴定,选择阳性克隆测序;对所获得的序列进行同源性和种系进化分析.结果获得的旱獭β-actin序列为349 bp（nt887～1 235）,其中编码序列为323 bp（nt-887～1209）,编码106个氨基酸,包含形成二硫键的2个丝氨酸位点（氨基酸16和氨基酸105）及与β-actin功能相关的2个ATP结合位点、6个凝溶胶蛋白结合位点和6个profilin结合位点.同源性分析发现上述序列与其它哺乳动物β-actin的同源性均高达88 %以上,与土拨鼠β-actin的同源性最高（99.69 %）,其氨基酸序列的同源性为100 %.种系进化树分析提示喜马拉雅旱獭β-actin与土拨鼠β-actin的亲缘关系最近,其次为其它啮齿类动物.结论成功克隆了喜马拉雅旱獭β-actin的部分序列.序列分析发现喜马拉雅旱獭β-actin与土拨鼠β-actin的同源性最高.

关键词：乙型肝炎病毒喜马拉雅旱獭肌动蛋白序列分析

Reveal the pharmacodynamic substances and mechanism of an edible medicinal plant Rhodiola crenulate in DSS-induced colitis through plasma pharmacochemistry and metabolomics

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Food Science and Human Wellness 2024年第4期13卷 2116-2131页

作者： Yu Peng Xiaoao Xiao Tingting Ji Xinyuan Wang Yixuan Xu Jianbo Xiao Hui Cao Zhiyong Chen Huifan Liu yuanqing Gao Hongxun tao Key Laboratory of Cardiovascular and Cerebrovascular Medicine School of PharmacyNanjing Medical UniversityNanjing 211166China School of Food and Biological Engineering Jiangsu UniversityZhenjiang 212013China Department of Analytical Chemistry and Food Science Faculty of ScienceUniversity of VigoVigo 36310Spain CISPAC Fontan BuildingCity of CultureSantiago de Compostela 15707Spain Shaanxi Academy of Traditional Chinese Medicine Xi'an 710003China Guangdong Key Laboratory of Science and Technology of Lingnan Specialty Food Zhongkai University of Agriculture and EngineeringGuangzhou 510550China State Key Laboratory of Southwestern Chinese Medicine Resources Chengdu 610075China

Rhodiola crenulate is the edible medicinal herbal medicine widely used for altitude sickness in ***,our previous work has found that *** extract(RCE)could significantly improve the pathology associated with dextran sulfate sodium-induced ***,the current research aims to reveal the pharmacodynamic material basis of RCE,as well as its mechanism against *** chemical characterization of RCE was performed by UHPLC-HR-MS,through which a total of 88 constituents were ***,our results also found 29 constituents absorbed into blood and 8 metabolized absorbable *** decreased flavonoids prototype and the elevated sulfated products of phenols were observed under pathophysiological conditions of *** metabolomics study revealed that colitis caused the alternation of fatty acid metabolism,steroid hormone biosynthesis and bile acid ***,RCE could prevent colitis by improving fatty acid metabolism and secondary bile acid metabolism.

关键词： Rhodiola crenulate Colitis Chemical characterization Plasma pharmacochemistry Metabolomics

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Defects‑Rich Heterostructures Trigger Strong Polarization Coupling in Sulfides/Carbon Composites with Robust Electromagnetic Wave Absorption

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Nano-Micro Letters 2025年第1期17卷 528-547页

作者： Jiaolong Liu Siyu Zhang Dan Qu Xuejiao Zhou Moxuan Yin Chenxuan Wang Xuelin Zhang Sichen Li Peijun Zhang Yuqi Zhou Kai tao Mengyang Li Bing Wei Hongjing Wu Mengyang Li Bing Wei Hongjing Wu School of Physics Xidian UniversityXi’an 710071People’s Republic of China School of Advanced Materials and Nanotechnology State Key Discipline Laboratory of Wide Band Gap Semiconductor TechnologyXidian UniversityXi’an 710071People’s Republic of China School of Microelectronics Xidian UniversityXi’an 710071People’s Republic of China School of Telecommunication Engineering Xidian UniversityXi’an 710071People’s Republic of China MOE Key Laboratory of Material Physics and Chemistry Under Extraordinary School of Physical Science and TechnologyNorthwestern Polytechnical UniversityXi’an 710072People’s Republic of China The Ministry of Education Key Laboratory of Micro and Nano Systems forAerospace School of Mechanical EngineeringNorthwestern PolytechnicalUniversityXi’an 710072People’s Republic of China

Defects-rich heterointerfaces integrated with adjustable crystalline phases and atom vacancies,as well as veiled dielectric-responsive character,are instrumental in electromagnetic *** methods,however,constrain their delicate ***,an innovative alternative is proposed:carrageenan-assistant cations-regulated(CACR)strategy,which induces a series of sulfides nanoparticles rooted in situ on the surface of carbon *** unique configuration originates from strategic vacancy formation energy of sulfides and strong sulfides-carbon support interaction,benefiting the delicate construction of defects-rich heterostructures in M_(x)S_(y)/carbon composites(M-CAs).Impressively,these generated sulfur vacancies are firstly found to strengthen electron accumulation/consumption ability at heterointerfaces and,simultaneously,induct local asymmetry of electronic structure to evoke large dipole moment,ultimately leading to polarization coupling,i.e.,defect-type interfacial ***“Janus effect”(Janus effect means versatility,as in the Greek two-headed Janus)of interfacial sulfur vacancies is intuitively confirmed by both theoretical and experimental investigations for the first ***,the sulfur vacancies-rich heterostructured Co/Ni-CAs displays broad absorption bandwidth of 6.76 GHz at only 1.8 mm,compared to sulfur vacancies-free CAs without any dielectric *** defects-rich heterostructures,this one-pot CACR strategy may steer the design and development of advanced nanomaterials,boosting functionality across diverse application domains beyond electromagnetic response.

关键词： Defects-rich heterointerfaces Sulfides Polarization coupling Electromagnetic wave absorption

Estimation of Chloride Diffusivity in Hydrated Tricalcium Silicate Using a Hydration-Diffusion Integrated Method

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Journal of Wuhan University of Technology(Materials Science) 2025年第1期 49-64页

作者：汪鑫 SHEN Dejian tao Sijie LIU Ruixin WU Shengxing College of Civil and Transportation Engineering Hohai University

This study aims to develop a chloride diffusion simulation method that considers the hydration microstructure and pore solution properties during the hydration of tricalcium silicate （C3S）.The method combines the hydration simulation,thermodynamic calculation,and finite element analysis to examine the effects of pore solution,including effect of electrochemical potential,effect of chemical activity,and effect of mechanical interactions between ions,on the chloride effective diffusion coefficient of hydrated C3S *** results indicate that the effect of electrochemical potential on chloride diffusion becomes stronger with increasing hydration age due to the increase in the content of hydrated calcium silicate;as the hydration age increases,the effect of chemical activity on chloride diffusion weakens when the number of diffusible elements decreases;the effect of mechanical interactions between ions on chloride diffusion decreases with the increase of hydration age.

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深度教学中直抵地理思想方法的课堂追问探究

教学与管理 2025年第1期 72-76页

作者：刘导黄东涛深圳市宝安区教育科学研究院广东深圳518000 汕头市金山中学广东汕头515100

深度教学中直抵地理思想方法的课堂追问是学生告别浅表的知识符号学习,不断深入发现、解释、提炼和总结地理规律与原理的过程。审视地理深度教学中的追问现状,地理思想方法在课堂追问中的运用存在着无结构、无梯度、无深度的实然状态。... 详细信息

深度教学中直抵地理思想方法的课堂追问是学生告别浅表的知识符号学习,不断深入发现、解释、提炼和总结地理规律与原理的过程。审视地理深度教学中的追问现状,地理思想方法在课堂追问中的运用存在着无结构、无梯度、无深度的实然状态。直抵地理思想方法的深度教学追问应具有溯源性、系统性、渐进性、开放性、辩证性、迁移性等样态特征。在地理思想方法的关照下,深度追问具有自身的发生时机和发生步骤,并以地理思想方法中的学科内涵、地理专家研究成果或是地理空间结构表达为抓手,落实形成地理深度教学中课堂追问的过程和方法。

关键词：深度教学地理思想方法课堂追问

Molecule‑Level Multiscale Design of Nonflammable Gel Polymer Electrolyte to Build Stable SEI/CEI for Lithium Metal Battery

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Nano-Micro Letters 2025年第1期17卷 404-423页

作者： Qiqi Sun Zelong Gong tao Zhang Jiafeng Li Xianli Zhu Ruixiao Zhu Lingxu Wang Leyuan Ma Xuehui Li Miaofa Yuan Zhiwei Zhang Luyuan Zhang Zhao Qian Longwei Yin Rajeev Ahuja Chengxiang Wang Key Laboratory for Liquid‑Solid Structural Evolution and Processing of Materials(Ministry of Education) School of Materials Science and EngineeringShandong UniversityJinan 250061People’s Republic of China Condensed Matter Theory Department of Physics and AstronomyUppsala UniversityUppsala 75120Sweden

The risk of flammability is an unavoidable issue for gel polymer electrolytes(GPEs).Usually,flameretardant solvents are necessary to be used,but most of them would react with anode/cathode easily and cause serious interfacial instability,which is a big challenge for design and application of nonflammable ***,a nonflammable GPE(SGPE)is developed by in situ polymerizing trifluoroethyl methacrylate(TFMA)monomers with flame-retardant triethyl phosphate(TEP)solvents and LiTFSI–LiDFOB dual lithium *** is strongly anchored to PTFMA matrix via polarity interaction between-P=O and-CH_(2)CF_(3).It reduces free TEP molecules,which obviously mitigates interfacial reactions,and enhances flame-retardant performance of TEP *** TEP molecules are also inhibited in solvation of Li^(+),leading to anion-dominated solvation sheath,which creates inorganic-rich solid electrolyte interface/cathode electrolyte interface *** coordination structure changes Li^(+)transport from sluggish vehicular to fast structural transport,raising ionic conductivity to 1.03 mS cm^(-1) and transfer number to 0.41 at 30℃.The Li|SGPE|Li cell presents highly reversible Li stripping/plating performance for over 1000 h at 0.1 mA cm^(−2),and 4.2 V LiCoO_(2)|SGPE|Li battery delivers high average specific capacity>120 mAh g^(−1) over 200 *** study paves a new way to make nonflammable GPE that is compatible with Li metal anode.

关键词： Anchoring effect Nonflammable gel electrolyte In situ cross-linked Electrode-electrolyte interface Li metal battery

Spatial distribution and temporal trends in potential evapotranspiration over Hengduan Mountains region from 1960 to 2009

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Journal of Geographical Sciences 2012年第1期22卷 71-85页

作者： ZHU Guofeng HE yuanqing PU tao WANG Xufeng JIA Wenxiong LI Zongxing XIN Huijuan State Key Laboratory of Cryosphere Sciences Yulong Snow Mountain Glaciers and Environmental Obser-vation Station Cold and Arid Regions Environmental and Engineering Research Institute CAS Graduate University of Chinese Academy of Sciences Research School of Arid Environment and Climate Change Lanzhou University College of Geography and Environment Science Northwest Normal University

Based on the meteorological data of 20 stations in the Hengduan Mountains region during 1961–2009, the annual and seasonal variation of potential evapotranspiration was analyzed in combination with the Penman-Monteith model. With the method of Spline interpolation under ArcGIS, the spatial distribution of potential evapotranspiration was presented to research the regional difference, and the correlation analysis was used to discuss the dominant factor affecting the potential evapotranspiration. The results indicated that the an-nual potential evapotranspiration showed a decreasing tendency since the 1960s, especially from the 1980s to 1990s, while it showed an increasing tendency since 2000. Regional potential evapotranspiration showed a rate of –0.17 mm a？1. Potential evapotranspiration in north, middle and south of the Hengduan Mountains exhibited decreasing trends over the studied period, and its regional trend was on the decline from southwest to northeast.

关键词： potential evapotranspiration Spline interpolation Penman-Monteith model spatial and temporal variations Hengduan Mountains

中国旱獭β2m基因的克隆、原核表达及多克隆抗体的制备

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医学分子生物学杂志 2009年第4期6卷 283-286页

作者：郝友华杨新星陈玲丁红晖王宝菊陶元清王忠东王虎陆蒙吉杨东亮华中科技大学同济医学院附属同济医院临床免疫研究室武汉市430030 青海省地方病预防控制所青海省实验动物中心西宁市810006 杜伊斯堡-埃森大学病毒学研究所德国杜伊斯堡45122

目的克隆、原核表达中国旱獭β2m基因，并制备多克隆抗体。方法利用RT—PCR技术从中国旱獭脾细胞中扩增β2m基因，克隆至pET28a（＋）质粒，构建原核表达载体pET-28a（＋）-CWβ2m，再转化宿主菌Rosetta（DE3）pLacI诱导其表达。使用切... 详细信息

目的克隆、原核表达中国旱獭β2m基因，并制备多克隆抗体。方法利用RT—PCR技术从中国旱獭脾细胞中扩增β2m基因，克隆至pET28a（＋）质粒，构建原核表达载体pET-28a（＋）-CWβ2m，再转化宿主菌Rosetta（DE3）pLacI诱导其表达。使用切胶回收纯化目的蛋白，将纯化蛋白免疫家兔制备多克隆抗体，并采用酶联免疫吸附实验检测抗体的灵敏度和特异性。结果克隆出的中国旱獭β2m基因，与GenBank已公布的土拨鼠的碱基序列一致；Western印迹结果显示克隆的β2m基因能在大肠埃希菌中高效表达，免疫家兔获得了高效价的多克隆抗体。结论成功克隆了中国旱獭β2m基因，在原核宿主中进行了高效表达，获得的多克隆抗体具有较高的效价。为人工制备MHC-Ⅰ类分子复合物，深入研究嗜肝病毒感染过程中特异性CTL应答和效应机制奠定了基础。

关键词：嗜肝病毒 β2m基因基因工程技术多克隆抗体

Science opportunities and challenges associated with SKA big data

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Science China(Physics,Mechanics & Astronomy) 2019年第8期62卷 121-126页

作者： tao An Shanghai Astronomical Observatory Key Laboratory of Radio Astronomy Chinese Academy of Sciences

The upcoming Square Kilometre Array (SKA) radio telescope will become the largest astronomical observation facility,and is expected to introduce revolutionary changes in major fields of natural *** revolutionary changes help us to answer the fundamental questions related to the origins of the universe,life,cosmic magnetic field,the nature of gravity,and to search for extraterrestrial civilizations [1].

关键词： Square Kilometre Array(SKA) introduce revolutionary changes fundamental questions related