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Acta Pharmaceutica Sinica B 2024年第4期14卷 1592-1604页

作者： Yuanjin Zhang Junze Huang Shengbo Huang Jie Liu Luyao Deng Chenmeizi Liang yuanqing guo Bingyi Yao Xin Wang Changning Maternity and Infant Health Hospital and School of Life Sciences Shanghai Key Laboratory of Regulatory BiologyEast China Normal UniversityShanghai 200241China

Organic anion-transporting polypeptides IB1(OATPIB1)plays a crucial role in the transport of ***,there are too few animal models related to OATPIB1,especially humanized animal *** this study,the human SLCOIB1 cDNA was inserted into the second exon of the rat Slcolb2 gene using CRISPR/Cas9 *** characteristics of statins were conducted in wild-type(WT),humanized OATPIB1(hOATPIB1),and OATPIB2 knockout(OATPIB2 KO)rats,*** results showed that human OATPIB1 was successfully expressed in rat liver and exhibited transport ***,the pharmacokinetic results revealed that OATPIB1 exhibited varying uptake levels of pivastatin,rosuvastatin,and fluvastatin,leading to different levels of exposure within the *** results were consistent with those obtained from in vitro experiments using overexpressed cell *** conclusion,we established a novel humanized SLCOIBI transgenic rat model to assess the role of human OATPIB1 in the uptake of different *** different uptake mediated by OATPIB1 may be an important reason for the different efficacy of *** hOATPIB1 rat is a promising model for improving the prediction of human drug transport.

关键词： CRISPR/Cas9 Drug transport Drug disposition Geneediting Humanized rat model OATP1B1 SLCO1B1 Statins

CRISPR-Cas9: A method for establishing rat models of drug metabolism and pharmacokinetics

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Acta Pharmaceutica Sinica B 2021年第10期11卷 2973-2982页

作者： Jian Lu Jie Liu yuanqing guo Yuanjin Zhang Yeye Xu Xin Wang Changning Maternity and Infant Health Hospital East China Normal UniversityShanghai 200051China Shanghai Key Laboratory of Regulatory Biology Institute of Biomedical Sciences and School of Life SciencesEast China Normal UniversityShanghai 200241China

The 2020 Nobel Prize in Chemistry recognized CRISPR-Cas9,a super-selective and precise gene editing ***-Cas9 has an obvious advantage in editing multiple genes in the same cell,and presents great potential in disease treatment and animal model *** recent years,CRISPRCas9 has been used to establish a series of rat models of drug metabolism and pharmacokinetics(DMPK),such as Cyp,Abcb1,Oatp1 b2 gene knockout *** new rat models are not only widely used in the study of drug metabolism,chemical toxicity,and carcinogenicity,but also promote the study of DMPK related mechanism,and further strengthen the relationship between drug metabolism and pharmacology/*** review systematically introduces the advantages and disadvantages of CRISPR-Cas9,summarizes the methods of establishing DMPK rat models,discusses the main challenges in this field,and proposes strategies to overcome these problems.

关键词： CRISPR-Cas9 Drug metabolism Pharmacokinetics Animal model Gene editing

HNF1B基因新发变异致胎儿肾囊肿的遗传学分析

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中国优生与遗传杂志 2024年第4期32卷 802-807页

作者：冯丽云郭苑青周毓青马海霞上海市长宁区妇幼保健院产前诊断中心上海200050

目的探讨1例先天性肾囊肿胎儿的临床特征及基因变异特点。方法收集胎儿的临床资料,应用染色体核型分析、低深度全基因组拷贝数变异测序(CNV-seq)检测和家系全外显子组测序(trio-WES)技术对胎儿及其父母进行遗传学分析,对候选变异进行San... 详细信息

目的探讨1例先天性肾囊肿胎儿的临床特征及基因变异特点。方法收集胎儿的临床资料,应用染色体核型分析、低深度全基因组拷贝数变异测序(CNV-seq)检测和家系全外显子组测序(trio-WES)技术对胎儿及其父母进行遗传学分析,对候选变异进行Sanger测序家系验证,应用生物信息学工具评估变异氨基酸保守性,分析蛋白质结构改变,预测变异蛋白致病性。结果胎儿系统超声检查提示双侧多发肾囊肿。核型分析未检出染色体数目或结构异常。CNV-seq检测未发现非整倍体或与胎儿表型相关的致病性拷贝数变异。trio-WES发现胎儿携带HNF1B基因NM_000458.2:c.1120dupA(***374Asnfs^(*)26)移码变异,Sanger测序验证该变异为新发变异,变异氨基酸在多个物种中高度保守,变异HNF1B蛋白结构受损,缺失了尾部的关键结构域。结合胎儿临床表型及遗传学检测结果,考虑诊断为HNF1B基因新发变异导致的常染色体显性遗传性HNF1-β肾病。结论HNF1B基因c.1120dupA新发移码变异是胎儿多发肾囊肿的遗传学病因,对生后表型具有重要预测作用。本研究丰富了HNF1B基因的致病变异谱。

关键词：先天性肾囊肿 HNF1B 全外显子组测序常染色体显性遗传胎儿

染色体核型分析联合CNV-Seq检测在高龄孕妇产前诊断中的应用

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检验医学与临床 2024年第4期21卷 507-510页

作者：马海霞冯丽云郭苑青何丽梅上海市长宁区妇幼保健院检验科上海200051 上海市长宁区妇幼保健院产筛中心上海200051

目的探讨染色体核型分析与全基因组拷贝数变异测序(CNV-Seq)技术在高龄孕妇产前诊断中联合应用的意义。方法对2020年1月至2022年12月该院收治的723例高龄孕妇的羊水细胞染色体核型分析、CNV-Seq检测结果进行回顾性分析。结果723例高龄... 详细信息

目的探讨染色体核型分析与全基因组拷贝数变异测序(CNV-Seq)技术在高龄孕妇产前诊断中联合应用的意义。方法对2020年1月至2022年12月该院收治的723例高龄孕妇的羊水细胞染色体核型分析、CNV-Seq检测结果进行回顾性分析。结果723例高龄孕妇中检出染色体异常共29例,异常检出率为4.0%。723例高龄孕妇中检出核型异常21例,异常检出率为2.9%,其中染色体非整倍体13例,包括21-三体7例,18-三体1例,性染色体非整倍体5例;染色体嵌合3例,包括性染色体嵌合2例,常染色体嵌合1例;染色体结构异常5例,包括染色体倒位3例,染色体易位2例;检出CNV-Seq异常24例,异常检出率为3.3%,其中染色体非整倍体13例,包括21-三体7例,18-三体1例,性染色体非整倍体5例;染色体嵌合3例,包括性染色体嵌合2例,常染色体嵌合1例;致病性染色体拷贝数变异8例,包括染色体微重复1例,染色体微缺失7例。其中染色体非整倍体异常中染色体核型分析和CNV-Seq检测结果一致。结论染色体核型分析联合CNV-Seq检测可提高染色体异常检出率,两种方法各自有独特的优势,可以相互验证,互补不足,染色体核型分析可比较直观地检测出染色体结构变异,而CNV-Seq可检测出染色体微缺失、微重复。

关键词：染色体核型分析全基因组拷贝数变异测序高龄孕妇产前诊断

A novel differential velocity modulation laser spectroscopy

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Chinese Science Bulletin 2001年第2期46卷 173-176页

作者： LIU Yuyan GAO Hui LIU Hongping DUAN Chuanxi LIN Jieli guo yuanqing LIU Xiaoyong HUANG Guangming LI Jinrui LI Fengyan State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics Wuhan Institute of Physics and MathematicsWuhan 430071China Department of Physics East China Normal UniversityShanghai 200062China Department of Physics Huazhong Normal UniversityWuhan 430079China Deparment of Physics Wuhan UniversityWuhan 430072China

Experimental investigation of a novel differential velocity modulation laser spectroscopy is reported and demonstrated with the spectra of N2+A2IIm-X2∑g+ Meinel system. The S/N ratio excesses 500 : 1, about 60 times higher than that with the traditional non-differential *** this technique, we obtained the high-resolution electronic absorption spectra of A2II-X2∑+(1,0) vibration-al band of CS+ for the first time. It is confirmed that this technique will be a powerful method and receive wide application in studies of new molecular ions.

关键词： velocity modulation differential technique molecular spectroscopy.

Innovations in metallurgical and materials processes:advancing liquid theory and honoring ***-jie guo's contributions to physical chemistry in metallurgy

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Journal of Iron and Steel Research International 2024年第1期31卷 1-2页

作者： Shuai Liu Jing guo Han-jie guo School of Artificial Intelligence Beijing Technology and Business UniversityBeijing100048China School of Metallurgical and Ecological Engineering University of Science and Technology Beijing(USTB)Beijing100083China

Spanning three decades,***-jie guo's research group has dedicated itself to exploring the fundamental theories and practical applications of physical chemistry within the realm of *** under the assumption that molecul... 详细信息

关键词： chemistry liquid complexes

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连续过滤-超滤工艺处理松花江水中试研究

哈尔滨工业大学学报 2019年第2期51卷 8-15页

作者：黄乔津郭远庆梁恒李圭白城市水资源与水环境国家重点实验室(哈尔滨工业大学) 哈尔滨150090

为探究以超滤为核心的短流程、低药剂的绿色净水工艺可行性,以连续过滤作为膜前预处理工艺,通过中试实验考察连续过滤-超滤工艺处理松花江水的处理效能和膜污染特性,并与直接超滤进行对比实验,考察连续过滤预处理在组合工艺中的作用.结... 详细信息

为探究以超滤为核心的短流程、低药剂的绿色净水工艺可行性,以连续过滤作为膜前预处理工艺,通过中试实验考察连续过滤-超滤工艺处理松花江水的处理效能和膜污染特性,并与直接超滤进行对比实验,考察连续过滤预处理在组合工艺中的作用.结果表明,连续过滤-超滤工艺对松花江水的浊度和氨氮均有良好的去除效果,去除率分别为99. 75%和70. 77%,但对有机物的去除效果较差,COD_(Mn)和UV_(254)的去除率分别为32. 21%和17. 12%.超滤膜前期污染较缓慢,15 d后污染速率加快,在第50天跨膜压差增长至50. 3 k Pa,达到膜污染上限.连续过滤对色氨酸类蛋白质有较好的去除效果,而超滤对溶解性生物代谢物、色氨酸类蛋白质、富里酸类有机物和腐殖酸类有机物均有一定的截留作用,其中腐殖酸类有机物和色氨酸类蛋白质是超滤膜的主要不可逆污染物.组合工艺对COD_(Mn)、UV_(254)和氨氮的去除率比直接过滤分别高出9. 97%,7. 02%和33. 84%,且膜污染速率远低于直接超滤.研究成果将推动超滤膜技术在微污染源水净化方面的广泛应用.

关键词：超滤连续过滤预处理膜污染绿色净水工艺

A review on DFACS(II):Modeling and analysis of disturbances and noises

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Chinese Journal of Aeronautics 2024年第5期37卷 120-147页

作者： Chenglei YUE Bohan JIAO Zhaohui DANG Xiaokui YUE Yonghe ZHANG yuanqing XIA Li DUAN Qinglei HU Qifan LIU Pengcheng WANG Ming guo Zhansheng DUAN Bing CUI Chu ZHANG Xiaodong SHAO School of Astronautics Northwestern Polytechnical UniversityXi’an 710072China Innovation Academy for Microsatellites Chinese Academy of SciencesShanghai 710699China School of Automation Beijing Institute of TechnologyBeijing 100081China Institute of Mechanics Chinese Academy of SciencesBeijing 710043China School of Automation Science and Electrical Engineering Beihang UniversityBeijing 100191China School of Automation Science and Engineering Xi’an Jiaotong UniversityXi’an 710049China

This paper presents Part II of a review on DFACS,which specifically focuses on the modeling and analysis of disturbances and noises in *** Part I,the system composition and dynamics model of the DFACS were *** this paper,we discuss the effects of disturbance forces and noises on the system,and summarize various analysis and modeling methods for these interferences,including the integral method,frequency domain analysis method,and magnitude evaluation *** analyzing the impact of disturbances and noises on the system,the paper also summarizes the system’s performance under slight ***,we highlight current research difficulties in the field of DFACS noise ***,this paper provides valuable insights into the modeling and analysis of disturbances and noises in DFACSs,and identifies key areas for future research.

关键词： Drag-free DFACS Disturbance Analysis Noise Analysis Frequency Domain Analysis Performance Evaluation

A review on DFACS(I):System design and dynamics modeling

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Chinese Journal of Aeronautics 2024年第5期37卷 92-119页

作者： Bohan JIAO Qifan LIU Zhaohui DANG Xiaokui YUE Yonghe ZHANG yuanqing XIA Li DUAN Qinglei HU Chenglei YUE Pengcheng WANG Ming guo Zhansheng DUAN Bing CUI Chu ZHANG Xiaodong SHAO School of Astronautics Northwestern Polytechnical UniversityXi’an 710072China Innovation Academy for Microsatellites Chinese Academy of SciencesShanghai 710699China School of Automation Beijing Institute of TechnologyBeijing 100081China Institute of Mechanics Chinese Academy of SciencesBeijing 710043China School of Automation Science and Electrical Engineering Beihang UniversityBeijing 100191China School of Automation Science and Engineering Xi’an Jiaotong UniversityXi’an 710049China

The Drag-Free and Attitude Control System(DFACS)is a critical platform for various space missions,including high precision satellite navigation,geoscience and gravity field measurement,and space scientific *** paper presents a comprehensive review of over sixty years of research on the design and dynamics model of ***,we examine the open literature on DFACS and its applications in Drag-Free missions,providing readers with necessary background information on the ***,we analyze the system configurations and main characteristics of different DFACSs,paying particular attention to the coupling mechanism between the system configuration and dynamics ***,we summarize the dynamics modeling methods and main dynamics models of DFACS from multiple perspectives,including common fundamentals and specific ***,we identify current challenges and technological difficulties in the system design and dynamics modeling of DFACS,while suggesting potential avenues for future *** paper aims to provide readers with a comprehensive understanding of the state-of-the-art in DFACS research,as well as the future prospects and challenges in this field.

关键词： Drag-Free DFACS System Design Dynamics Modeling Control design

Emerging direct current triboelectric nanogenerator for high-entropy mechanical energy harvesting

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Science China(Technological Sciences) 2024年第5期67卷 1297-1316页

作者： CHEN Jie guo RuiLong guo HengYu College of Physics and Electronic Engineering Chongqing Normal UniversityChongqing 401331China Department of Applied Physics Chongqing UniversityChongqing 401331China

In the era of the Internet of Things(IoT),the provision of sustainable power to distributed,mobile,and low-power-consumption electronic devices is a critical *** overcome this challenge,the triboelectric nanogenerator(TENG),a highly efficient high-entropy mechanical energy harvesting device,was developed in *** device enables the direct conversion of irregular and low-frequency mechanical energy into pulsed alternating current(AC)***,the incompatibility of most electronic devices with AC signals necessitates rectifier circuits or generators that deliver direct current(DC)*** recent years,DC-TENGs have undergone extensive development,achieving significant milestones in various application fields while also facing crucial challenges that require *** this review,three categories of DC-TENG devices with distinct operating mechanisms are comprehensively explored:multiphase coupling,mechanical rectification,and air *** typical structures and working mechanisms are thoroughly discussed,and specific output performance limitations,along with corresponding optimization strategies,are ***,the applications of DC-TENGs in various scenarios are ***,the challenges faced by DC-TENGs and potential solutions are analyzed to guide further advancements in this technology.

关键词： TENG direct current working mechanisms output performance limitations optimization strategies