检索结果-南通市图书馆

Establishment of an indirect immunofluorescence assay for the detection of African swine fever virus antibodies

Journal of Integrative Agriculture 2024年第1期23卷 228-238页

作者： Wan Wang Zhenjiang Zhang Weldu Tesfagaber Jiwen Zhang Fang Li Encheng Sun Lijie Tang Zhigao Bu yuanmao zhu Dongming Zhao State Key Laboratory for Animal Disease Control and Prevention National African Swine Fever Para-reference LaboratoryNational High Containment Facilities for Animal Diseases Control and PreventionHarbin Veterinary Research InstituteChinese Academy of Agricultural SciencesHarbin 150069China College of Veterinary Medicine Northeast Agricultural UniversityHarbin 150069China

African swine fever(ASF)continues to cause enormous economic loss to the global pig *** there is no safe and effective vaccine,accurate and timely diagnosis of ASF is essential to implement control *** immunofluorescence assay(IFA)is a gold standard serological method recommended by the World Organization for Animal Health(WOAH).In this study,we used primary fetal kidney cells to establish a wild boar cell line(BK2258)that supported the efficient replication of ASF virus(ASFV)SD/DY-I/21 and showed visible cytopathic effect(CPE).Moreover,using BK2258,we established a sensitive and specific IFA for ASFV antibody *** standardize and evaluate the performance of this assay,we used serum samples from pigs infected with the low virulent genotype I SD/DY-I/21 and genotype II HLJ/HRB1/20,and immunized with the vaccine candidate HLJ/18-7GD,field samples,and negative serum *** IFA reacted with the ASFV-positive sera and displayed bright fluorescence *** was no non-specific green fluorescence due to cellular senescence or other cell damage-causing *** to a commercial indirect enzyme-linked immunosorbent assay(iELISA),ASFV antibodies were detected 1–4 days earlier using our *** detection limits of the IFA and iELISA for the same ASFV-antibody positive serum samples were 1:25,600 and 1:6,400,respectively,indicating that the IFA is more sensitive than *** newly established IFA was highly specific and did not cross-react with sera positive for six other important porcine pathogens(i.e.,Classical swine fever virus(CSFV),Porcine reproductive and respiratory syndrome virus(PRRSV),Porcme circovirus type 2(PCV2),Pseudorabies virus(PRV),Foot-and-Mouth disease virus type O(FMDV/O),and Porcine epidemic diarrhea virus(PEDV)).This study thus provides a sensitive,specific,and reliable detection method that is suitable for the serological diagnosis of ASF.

关键词： African swine fever antibody IFA serological method

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

A CRISPR/Cas12a-based platform for rapid on-site bovine viral diarrhea virus diagnostics

引用

Journal of Integrative Agriculture 2024年第8期23卷 2872-2876页

作者： Meixi Wang Jitao Chang Yuxin Han Chaonan Wang Songkang Qin Jun Wang Lulu Zhang yuanmao zhu Fei Xue Fang Wang Hongliang Chai Yulong Wang Xinjie Wang Xin Yin State Key Laboratory for Animal Disease Control and Prevention Harbin Veterinary Research InstituteChinese Academy of Agricultural SciencesHarbin 150069China Shenzhen Branch Guangdong Laboratory of Lingnan Modern AgricultureGenome Analysis Laboratory of the Ministry of Agriculture and Rural AffairsAgricultural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesShenzhen 518000China College of Wildlife and Protected Area Northeast Forestry UniversityHarbin 150040China Institute of Western Agriculture Chinese Academy of Agricultural SciencesChangji 831100China

Bovine viral diarrhea virus(BVDV)is a positive-sense single-stranded RNA virus,belonging to the genus Pestivirus in the Flaviviridae family(Riitho et al.2020).Currently,BVDV is divided into 3 main genotypes,BVDV-1,BVD... 详细信息

关键词： BVDV CRISPR/Cas diarrhea

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Potassium alleviated high nitrogen-induced apple growth inhibition by regulating photosynthetic nitrogen allocation and enhancing nitrogen utilization capacity

引用

Horticultural Plant Journal 2024年第1期10卷 1-14页

作者： Xinxiang Xu Guangyuan Liu Jingquan Liu Mengxue Lyu Fen Wang Yue Xing Hao Meng Min Li Yu Jiang Ge Tian Zhanling zhu yuanmao Jiang Shunfeng Ge State Key Laboratory of Crop Biology College of Horticulture Science and EngineeringShandong Agricultural UniversityTai'anShandong 271018China Key Laboratory of Biochemistry and Molecular Biology in Universities of Shandong College of Biological and Agricultural EngineeringWeifang UniversityWeifangShandong 261061China

There is a close relationship between potassium(K)and nitrogen(N).However,the roles of K under high N conditions remain *** a hydroponics approach,we monitored the morphological,physiological,and molecular changes in M9T337 apple(Malus domestica)rootstocks under different nitrate(10 and 30 mmol·L^(-1)NO_(3)^(-))and K supply(0.5,6,10,and 20 mmol·L_(-1)K^(+))*** revealed that high nitrate inhibited the root growth of M9T337 rootstocks,downregulated the expressions of K transporter genes(MdPT5,MdHKT1,and MdATK1),and reduced the net NO3-and K+influx at the surface of roots,thereby resulting in an N/K imbalance in *** investigation showed that 10 mmol·L^(-1)K increased the activity of N metabolic enzymes(NR,GS,NiR,and GOGAT),upregulated the expressions of genes related to nitrate uptake and transport(MdNRT1.1,MdNRT1.2,MdNRT1.5,and MdNRT2.4),promoted15N transport from the roots to the shoots,optimized leaf N distribution,and improved photosynthetic N utilization efficiency under high nitrate *** results suggest that the negative effects of high nitrate may be related to the N/K imbalance and that reducing N/K in plants by increasing K supply level can effectively alleviate the inhibition of N assimilation by high nitrate stress.

关键词： Apple rootstock K level High nitrate stress N metabolism ^(15)N

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Novel P22-monoclonal antibody based blocking ELISA for the detection of African swine fever virus antibodies in serum

引用

Biosafety and Health 2022年第4期4卷 234-243页

作者： Ghebremedhin Tsegay Weldu Tesfagaber yuanmao zhu Xijun He Wan Wang Zhenjiang Zhang Encheng Sun Jinya Zhang Yuntao Guan Fang Li Renqiang Liu Zhigao Bu Dongming Zhao State Key Laboratory of Veterinary Biotechnology National High Containment Facilities for Animal Diseases Control and PreventionNational African Swine Fever Para-reference LaboratoryHarbin Veterinary Research InstituteChinese Academy of Agricultural SciencesHarbin 150069China Department of Veterinary Science Hamelmalo Agricultural CollegeKerenEritrea

African swine fever(ASF)is a highly infectious,transboundary viral disease of domestic and wild pigs,and is currently the most serious threat to world swine production,resulting in significant economic *** the absence of vaccines and treatments,the control of the disease entirely depends on accurate and early diagnosis accompanied by the culling of infected ***,a highly specific and sensitive diagnostic assay is required during an outbreak and surveillance of the *** this study,a highly sensitive,specific,rapid and repeatable P22-monoclonal antibody-based blocking enzyme-linked immunosorbent assay(bELISA)assay was developed for the detection of antibodies against genotype I and II African swine fever viruses(ASFVs).A total of 806 pig serum samples were tested to evaluate the performance of the diagnostic *** determine the PI(percent Inhibition)cut-off value,receiver-operating characteristic(ROC)analysis was *** to the ROC analysis of the data,98.10%specificity and 100%sensitivity were recorded when the threshold cut-off value of PI was established at 47%.In addition,the assay was able to detect ASFV antibodies as early as 9 days post-infection when serum samples from experimentally infected pigs were *** all together,the results of the present study indicated that the P22-mAb based bELISA assay can be used for rapid and accurate detection of antibodies against ASFV,which could play a valuable role in the containment and prevention of ASFV as an alternative to other serological diagnostic ***,this study will assist researchers to further investigate the immunogenic importance of P22 protein in ASFV infection.

关键词： Monoclonal antibodies African swine fever Blocking ELISA Diagnosis P22

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Carbon Emission Effects Driven by Evolution of Chinese Dietary Structure from 1987 to 2020

引用

Chinese Geographical Science 2024年第1期34卷 181-194页

作者： zhu Yuanyuan ZHANG Yan zhu Xiaohua Key Laboratory of Geographic Process Analysis&Simulation Hubei Province Central China Normal UniversityWuhan430079China Institute of Geographic Sciences and Natural Resources Research Chinese Academy of SciencesBeijing100101China

Exploring carbon emission effects based on the evolution of residents’ dietary structure to achieve the carbon neutrality goal and mitigate climate change is an important *** study took China as the research object(data excluding Hong Kong,Macao and Taiwan) and used the carbon emission coefficient method to quantitatively measure the food carbon emissions from 1987–2020,then analyzed the carbon emission effects under the evolution of dietary *** results showed that during the study period,the Chinese dietary structure gradually changed to a high-carbon consumption *** dietary structure of urban residents developed to a balanced one,while that of rural residents developed to a high-quality *** the study period,the per capita food carbon emissions and total food consumption of Chinese showed an increasing *** per capita food carbon emissions of residents in urban and rural showed an overall upward *** total food carbon emissions in urban increased significantly,while that in rural increased first and then *** influence of beef and mutton on carbon emissions is the highest in dietary *** with the balanced dietary pattern,the food carbon emissions of Chinese residents had not yet reached the peak,but were evolving to a high-carbon consumption pattern.

关键词： dietary structure structural evolution carbon emission effects carbon neutrality China

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Synergistic catalysis of the N-hydroxyphthalimide on flower-like bimetallic metal-organic frameworks for boosting oxidative desulfurization

引用

Petroleum Science 2024年第1期21卷 674-682页

作者： Jing He Kun zhu Wei Jiang Dong-Ao zhu Lin-Hua zhu Hai-Yan Huang Wen-Shuai zhu Hua-Ming Li Institute for Energy Research Jiangsu UniversityZhenjiang212013JiangsuChina College of Chemistry and Chemical Engineering Hainan Normal UniversityKey Laboratory of Water Pollution Treatment and Resource Reuse of Hainan ProvinceHaikou571158HainanChina

Synergic catalytic effect between active sites and supports greatly determines the catalytic activity for the aerobic oxidative desulfurization of fuel *** this work,Ni-doped Co-based bimetallic metal-organic framework(CoNi-MOF)is fabricated to disperse N-hydroxyphthalimide(NHPI),in which the whole catalyst provides plentiful synergic catalytic effect to improve the performance of oxidative desulfurization(ODS).As a bimetallic MOF,the second metal Ni doping results in the flower-like morphology and the modification of electronic properties,which ensure the exposure of NHPI and strengthen the synergistic effect of the overall *** with the monometallic Co-MOF and naked NHPI,the NHPI@CoNi-MOF triggers the efficient activation of molecular oxygen and improves the ODS performance without an *** sulfur removal of dibenzothiophene-based model oil reaches 96.4%over the NHPI@CoNi-MOF catalyst in 8 h of ***,the catalytic product of this aerobic ODS reaction is sulfone,which is adsorbed on the catalyst surface due to the difference in *** work provides new insight and strategy for the design of a strong synergic catalytic effect between NHPI and bimetallic supports toward high-activity aerobic ODS materials.

关键词： Metal-organic frameworks Doped Bimetallic N-hydroxyphthalimide Aerobic processes Oxidative desulfurization

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

A review of recent advances in the effects of surface and interface properties on marine propellers

引用

Friction 2024年第2期12卷 185-214页

作者： Manfu zhu Liran MA State Key Laboratory of Tribology in Advanced Equipment Tsinghua UniversityBeijing 100084China

Marine propellers are important propulsion devices for both surface ships and underwater *** severe environmental problems have required further performance enhancement for ***,traditional methods to improve propeller performances through geometrical and structural optimizations have been extensively investigated,while the underlying mechanisms of the effects of surface and interface properties on marine propellers are still far from being fully *** paper presented a comprehensive review of recent advances in the effects of surface and interface properties,such as surface roughness and surface wettability,on marine propellers with an emphasis on the significant improvements in both hydrodynamic and cavitation performances,hoping to arouse more in-depth investigations in the field of surface/interface science and technologies on marine propellers,and also promote the state-of-the-art technologies,such as superlubricity technology,into practical applications.

关键词： marine propellers hydrodynamics cavitation surface roughness surface wettability

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Engineering applications and technical challenges of active array microsystems

引用

Frontiers of Information Technology & Electronic Engineering 2024年第3期25卷 342-368页

作者： Jiaguo LU Haoran zhu East China Research Institute of Electronic Engineering Hefei 230039China School of Electronic and Information Engineering Anhui UniversityHefei 230601China

In the post-Moore era,the development of active phased array antennas will inevitably trend towards active array *** this paper,the characteristics and composition of the active array antenna are briefly *** to the high efficiency,low profile,and light weight of the active array microsystems,the application prospects and advantages in the engineering of multi-functional airborne radar,spaceborne radar,and communication systems are ***,according to the characteristics of the post-Moore era of integrated circuits,scientific and technological problems in the active array microsystems are presented,including multi-scale,multi-signal,and multi-physics field *** challenges are also discussed,such as new architectures and algorithms,miniaturization of passive components,novel materials and processes,ultra-wideband technology,and new interdisciplinary technological *** paper is expected to inspire in-depth research on active array microsystems.

关键词： Microelectronics Heterogeneous integration Packaging materials Antenna array microsystems Multi-functional radar Communication

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Leaching kinetics of fluorine during the aluminum removal from spent Li-ion battery cathode materials

引用

Journal of Environmental Sciences 2024年第4期138卷 312-325页

作者： Shengjie Li Jianxin zhu Research Center for Eco-Environmental Sciences Chinese Academy of SciencesBeijing 100085China University of Chinese Academy of Sciences Beijing 100049China

The high content of aluminum(Al)impurity in the recycled cathode powder seriously affects the extraction efficiency of Nickel,Cobalt,Manganese,and Lithium resources and the actual commercial value of recycled materials,so Al removal is crucially important to conform to the industrial standard of spent Li-ion battery cathode *** this work,we systematically investigated the leaching process and optimum conditions associated with Al removal from the cathode powder materials collected in a wet cathode-powder peeling and recycling production line of spent Li-ion batteries(LIBs).Moreover,we specifically studied the leaching of fluorine(F)synergistically happened along with the removal process of Al,which was not concerned about in other studies,but one of the key factors affecting pollution prevention in the recovery *** mechanism of the whole process including the leaching of Al and F from the cathode powder was indicated by using NMR,FTIR,and XPS,and a defluoridation process was preliminarily investigated in this *** leaching kinetics of Al could be successfully described by the shrinking core model,controlled by the diffusion process and the activation energy was 11.14 kJ/***,the leaching of F was attributed to the dissolution of LiPF6and decomposition of PVDF,and the kinetics associated was described by Avrami *** interaction of Al and F is advantageous to realize the defluoridation to some *** is expected that our investigation will provide theoretical support for the large-scale recycling of spent LIBs.

关键词： Al removal F leaching Cathode powder Heating alkali leaching Leaching kinetics

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Paving the way toward soil safety and health:current status,challenges,and potential solutions

引用

Frontiers of Environmental Science & Engineering 2024年第6期18卷 63-73页

作者： Chiheng Chu Lizhong zhu College of Environmental and Resource Sciences Zhejiang UniversityHangzhou 310058China

Soil is a non-renewable resource,providing a majority of the world’s food and fiber while serving as a vital carbon ***,the health of soil faces global threats from human activities,particularly widespread contamination by industrial *** physical,chemical,and biological remediation approaches encounter challenges in preserving soil structure and function throughout the remediation process,as well as addressing the complexities of soil contamination on a regional *** solutions encompass monitoring and simulating soil processes,with a focus on utilizing big data to bridge micro-scale and macro-scale ***,reducing pollutant emissions to soil is paramount due to the significant challenges associated with removing contaminants once they have entered the soil,coupled with the high economic costs of ***,it is imperative to implement advanced remediation technologies,such as monitored natural attenuation,and embrace holistic soil management approaches that involve regulatory frameworks,soil health indicators,and soil safety monitoring *** the enduring health and resilience of soils necessitates a blend of interdisciplinary research,technological innovation,and collaborative initiatives.

关键词： Soil safety and health Source emission reduction Process monitoring and simulation Green remediation technology Soil health management

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：