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Cross-reactivity of eight SARS-CoV-2 variants rationally predicts immunogenicity clustering in sarbecoviruses

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Signal Transduction and Targeted Therapy 2022年第8期7卷 3069-3079页

作者： Qianqian Li Li Zhang Ziteng Liang Nan Wang Shuo Liu Tao Li yuanling yu Qianqian Cui Xi Wu Jianhui Nie Jiajing Wu Zhimin Cui Qiong Lu Xiangxi Wang Weijin Huang Youchun Wang Division of HIV/AIDS and Sex-transmitted Virus Vaccines Institute for Biological Product ControlNational Institutes for Food and Drug Control(NIFDC)WHO Collaborating Center for Standardization and Evaluation of BiologicalsNHC Key Laboratory of Research on Quality and Standardization of Biotech Products and NMPA Key Laboratory for Quality Research and Evaluation of Biological Products102629 BeijingChina Jiangsu Recbio Technology Co. Ltd.215300 TaizhouChina Graduate School of Chinese Academy of Medical Sciences&Peking Union Medical College No.9 Dongdan SantiaoDongcheng District100730 BeijingChina CAS Key Laboratory of Infection and Immunity CAS Center for Excellence in BiomacromoleculesInstitute of BiophysicsChinese Academy of Sciences100101 BeijingChina

A steep rise in Omicron reinfection cases suggests that this variant has increased immune evasion *** evaluate its antigenicity relationship with other variants,antisera from guinea pigs immunized with spike protein of SARS-CoV-2 variants of concern(VOCs)and variants of interest(VOIs)were cross-tested against pseudotyped *** neutralization activity against Omicron was markedly reduced when other VOCs or VOIs were used as immunogens,and Omicron(BA.1)-elicited sera did not efficiently neutralize the other ***,a Beta or Omicron booster,when administered as the 4th dose 3-months after the 3rd dose of any of the variants,could elicit broad neutralizing antibodies against all of the current variants including Omicron *** analysis with 280 available antigen–antibody structures and quantification of immune escape from 715 reported neutralizing antibodies provide explanations for the observed differential *** distinct clades predicted using an in silico algorithm for clustering of sarbecoviruses based on immune escape provide key information for rational design of vaccines.

关键词： rational escape neutral

Spike N354 glycosylation augments SARS-CoV-2 fitness for human adaptation through structural plasticity

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National Science Review 2024年第7期11卷 80-94页

作者： Pan Liu Can yue Bo Meng Tianhe Xiao Sijie Yang Shuo Liu Fanchong Jian Qianhui Zhu yuanling yu Yanyan Ren Peng Wang Yixin Li Jinyue Wang Xin Mao Fei Shao Youchun Wang Ravindra Kumar Gupta yunlong Cao Xiangxi Wang CAS Key Laboratory of Infection and Immunity National Laboratory of MacromoleculesInstitute of BiophysicsChinese Academy of Sciences University of Chinese Academy of Sciences Cambridge Institute of Therapeutic Immunology&Infectious Disease(CITIID) University of Cambridge Biomedical Pioneering Innovation Center(BIOPIC) Peking University Changping Laboratory Joint Graduate Program of Peking-Tsinghua-NIBS Academy for Advanced Interdisciplinary Studies Peking University Peking-Tsinghua Center for Life Sciences Tsinghua University Chinese Academy of Medical Sciences&Peking Union Medical College

Selective pressures have given rise to a number of SARS-CoV-2 variants during the prolonged course of the COVID-19 pandemic. Recently evolved variants differ from ancestors in additional glycosylation within the spike protein receptor-binding domain(RBD). Details of how the acquisition of glycosylation impacts viral fitness and human adaptation are not clearly understood. Here, we dissected the role of N354-linked glycosylation, acquired by BA.2.86 sub-lineages, as a RBD conformational control element in attenuating viral infectivity. The reduced infectivity is recovered in the presence of heparin sulfate, which targets the‘N354 pocket’ to ease restrictions of conformational transition resulting in a ‘RBD-up’ state, thereby conferring an adjustable infectivity. Furthermore, N354 glycosylation improved spike cleavage and cell–cell fusion, and in particular escaped one subset of ADCC antibodies. Together with reduced immunogenicity in hybrid immunity background, these indicate a single spike amino acid glycosylation event provides selective advantage in humans through multiple mechanisms.

关键词： coronavirus glycosylation viral fitness adjustable infectivity co-factor usage viral evolution conformational modulator

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尿激酶治疗冠心病血浆D-二聚体的变化研究

临床心血管病杂志 1998年第1期14卷 21-23页

作者：余静骆雷鸣余远琳兰州医学院附属第二医院心内科 730030 兰州医学院附属第二医院血液科

应用ELISA双抗体夹心法，检测了31例冠心病患者血浆D-二聚体的含量。冠心病中急性心肌梗死（AMI）9例，不稳定型心绞痛（UAP）22例。结果：AMI及UAP患者血浆D-二聚体含量均高于对照组（P＜0．01），且AMI组高于UAP组（P＜0．05）。应用... 详细信息

应用ELISA双抗体夹心法，检测了31例冠心病患者血浆D-二聚体的含量。冠心病中急性心肌梗死（AMI）9例，不稳定型心绞痛（UAP）22例。结果：AMI及UAP患者血浆D-二聚体含量均高于对照组（P＜0．01），且AMI组高于UAP组（P＜0．05）。应用尿激酶治疗后，AMI组中血浆D-二聚体含量又再度升高（P＜0．01），以溶栓后6h升高最为显著。而UAP组1周内均无明显改变（P＞0．05）。提示：①AMI及UAP的交联纤维蛋白D-二聚体增多有血栓的形成和溶解；②AMI患者应用尿激酶溶栓治疗后，血浆D-二聚体再度升高，可为溶栓成功的指标之一，而小剂量尿激酶治疗UAP后，D-二聚体无明显变化。

关键词：冠心病 D-二聚体尿激酶

基于SARIMA-RF组合模型的西安中欧班列预测及发展对策

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重庆交通大学学报（自然科学版） 2024年第10期43卷 90-96页

作者：黄宝静马骏余元玲中国铁道科学研究院集团有限公司北京100081 中国铁路西安局集团有限公司陕西西安710054 重庆交通大学国际学院重庆400074

为提升西安中欧班列开行车数预测精度和泛化能力,综合考虑西安中欧班列时间序列数据的线性和非线性特征,提出基于SARIMA-RF组合模型的班列开行车数预测方法。首先使用季节性自回归移动平均模型(SARIMA)预测开行车数,其次利用随机森林(RF... 详细信息

为提升西安中欧班列开行车数预测精度和泛化能力,综合考虑西安中欧班列时间序列数据的线性和非线性特征,提出基于SARIMA-RF组合模型的班列开行车数预测方法。首先使用季节性自回归移动平均模型(SARIMA)预测开行车数,其次利用随机森林(RF)模型校正残差,构建组合模型,最后将组合模型与ARIMA、SARIMA、RF、XGBoost进行对比。使用2014-2023年西安中欧班列月度开行数据实验,预测2024年开行车数为24.40万车,2025年为26.71万车,对比结果表明:组合模型的M_(SE)、R_(MSE)、M_(AE)、M_(APE)分别为0.0037、0.0610、0.0530、3.41%,比其他模型精度更高。

关键词：交通运输工程 “长安号”中欧班列季节性波动 SARIMA-RF 残差校正发展对策

Antibody-dependent cellular cytotoxicity response to SARS-CoV-2 in COVID-19 patients

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Signal Transduction and Targeted Therapy 2021年第10期6卷 3132-3141页

作者： yuanling yu Meiyu Wang Xiaoai Zhang Shufen Li Qingbin Lu Haolong Zeng Hongyan Hou Hao Li Mengyi Zhang Fei Jiang Jiajing Wu Ruxia Ding Zehua Zhou Min Liu Weixue Si Tao Zhu Hangwen Li Jie Ma yuanyuan Gu Guangbiao She Xiaokun Li yulan Zhang Ke Peng Weijin Huang Wei Liu Youchun Wang Division of HIV/AIDS and Sex-transmitted Virus Vaccines Institute for Biological Product ControlNational Institutes for Food and Drug Control(NIFDC)BeijingChina Graduate School of Peking Union Medical College Chinese Academy of Medical SciencesPeking Union Medical CollegeBeijingChina State Key Laboratory of Pathogen and Biosecurity Beijing Institute of Microbiology and EpidemiologyBeijingChina State Key Laboratory of Virology Wuhan Institute of VirologyChinese Academy of SciencesWuhanHubeiChina Department of Laboratorial Science and Technology School of Public HealthPeking UniversityBeijingChina Department of Laboratory Medicine Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhanChina Sinovac Biotech Co. LtdBeijingChina Cansino Biotech Incorporation TianjinChina Stemirna Therapeutics LtdShanghaiChina Anhui Zhifeilongcom Biopharmaceutical Co. LtdHefeiChina University of Chinese Academy of Sciences BeijingChina

Antibody-dependent cellular cytotoxicity(ADCC)responses to viral infection are a form of antibody regulated immune responses mediated through the Fc *** severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)triggered ADCC responses contributes to COVID-19 disease development is currently not well *** understand the potential correlation between ADCC responses and COVID-19 disease development,we analyzed the ADCC activity and neutralizing antibody response in 255 individuals ranging from asymptomatic to fatal infections over 1 year post *** was elicited by 10 days post-infection,peaked by 11-20 days,and remained detectable until 400 days *** general,patients with severe disease had higher ADCC ***,patients who had severe disease and recovered had higher ADCC activities than patients who had severe disease and ***,ADCC activities were mediated by a diversity of epitopes in SARS-COV-2-infected mice and induced to comparable levels against SARS-CoV-2 variants of concern(VOCs)(B.1.1.7,B.1.351,and P.1)as that against the D614G mutant in human patients and vaccinated *** study indicates anti-SARS-CoV-2 ADCC as a major trait of COVID-19 patients with various conditions,which can be applied to estimate the extra-neutralization level against COVID-19,especially lethal COVID-19.

关键词： patients ADCC Antibody

人慢性根尖周炎及根尖囊肿组织巨噬细胞中IL-33的表达

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口腔疾病防治 2019年第5期27卷 300-303页

作者：代茜余苑玲黄世光暨南大学口腔医学院广东广州510632 暨南大学附属第一医院广东广州510632

目的观察白细胞介素-33(interleukin-33,IL-33)在慢性根尖周炎及根尖囊肿组织巨噬细胞中的表达情况,为研究IL-33在根尖周病发病机制中的作用提供基础。方法收集正畸减数拔牙的无病变人牙根尖组织20例为正常对照组,慢性根尖周炎组织15例... 详细信息

目的观察白细胞介素-33(interleukin-33,IL-33)在慢性根尖周炎及根尖囊肿组织巨噬细胞中的表达情况,为研究IL-33在根尖周病发病机制中的作用提供基础。方法收集正畸减数拔牙的无病变人牙根尖组织20例为正常对照组,慢性根尖周炎组织15例为慢性根尖周炎组,根尖囊肿组织15例为根尖囊肿组,分别进行HE染色,光学显微镜下观察根尖周组织的变化;以CD14作为巨噬细胞的标记,采用免疫荧光双染色法,在荧光显微镜下分别观察各组CD14阳性的巨噬细胞表达IL-33的情况。结果正常对照组、慢性根尖周炎组、根尖囊肿组中IL-33、CD14表达阳性的巨噬细胞密度分别为(23.81±5.16)个/mm^2、(62.97±8.54)个/mm^2、(119.83±14.61)个/mm^2;3组间差异具有统计学意义(F=87.17,P <0.01);根尖囊肿组IL-33、CD14表达阳性的巨噬细胞密度显著高于慢性根尖周炎组及对照组(P <0.01)。结论 IL-33、CD14表达阳性的巨噬细胞在正常根尖组织、慢性根尖周炎组织、根尖囊肿组织中依次增加。

关键词：白细胞介素-33 慢性根尖周炎根尖周囊肿 CD14 巨噬细胞

中欧班列高质量发展及产学研合作路径——第三届重庆与中欧班列沿线国家产学研合作国际论坛综述

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科技和产业 2024年第8期24卷 176-180页

作者：朱秋秋余元玲雷洋重庆交通大学经济与管理学院重庆400074 重庆交通大学欧洲研究中心重庆400074

第三届重庆与中欧班列沿线国家产学研合作国际论坛于2023年11月11日在重庆外事中心成功举办。100余名来自中国、哈萨克斯坦、匈牙利、法国和波兰等国家的产学研专家出席论坛。会议总结了中欧班列在班列运行、国际影响及沿线国家产学研... 详细信息

第三届重庆与中欧班列沿线国家产学研合作国际论坛于2023年11月11日在重庆外事中心成功举办。100余名来自中国、哈萨克斯坦、匈牙利、法国和波兰等国家的产学研专家出席论坛。会议总结了中欧班列在班列运行、国际影响及沿线国家产学研合作的成果,总结了发展经验。基于班列面临的挑战,为提升中欧班列高质量发展,与会专家基于最新研究提出建议。

关键词：中欧班列高质量发展产学研合作会议综述