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Ectopic expression of a male fertility gene,LOGL8,represses LOG and hinders panicle and ovule development

The Crop Journal 2022年第6期10卷 1665-1673页

作者： Shuifu chen Sulin Lou Xiucai Zhao Shijuan Zhang Letian chen Ping Huang Guande Li Yingying Li Yao-Guang Liu yuanling chen State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources South China Agricultural UniversityGuangzhou 510642GuangdongChina Key Laboratory of Plant Functional Genomics and Biotechnology of Guangdong Provincial Higher Education Institutions Guangzhou 510642GuangdongChina Guangdong Provincial Key Laboratory of Protein Function and Regulation in Agricultural Organisms South ChinaAgricultural UniversityGuangzhou 510642GuangdongChina College of Life Sciences South China Agricultural UniversityGuangzhou 510642GuangdongChina College of Life Sciences and Oceanography Shenzhen UniversityShenzhen 518060GuangdongChina Shenzhen Key Laboratory of Marine Bioresource and Eco-Environmental Science Shenzhen 518060GuangdongChina g Guangdong Provincial Key Laboratory for Plant Epigenetics Shenzhen 518060GuangdongChina

Grain number and seed-setting rate are components of crop yield.Cytokinin influences grain yield.However,emerging studies suggest that high cytokinin signals often lead to reduced branching or seed-setting rate,leading to reduced grain yield,although the mechanisms remain unclear.In this study,we identified and characterized the rice(Oryza sativa L.)gene LONELY GUY-LIKE 8(LOGL8),based on analysis of the LOGL8-pm(promoter mutant of LOGL8)mutant,which harbors a T-DNA insertion in the promoter of this gene.The mutation in LOGL8-pm causes ectopic hyperexpression of LOGL8 in inflorescence organs,resulting in plants with smaller panicles and defective ovules lacking archesporial cells and integuments.Knockout of LOGL8 caused pollen abortion,leading to a reduced seed-setting rate.LOGL8 encodes a putative cytokinin-activating enzyme.Our results showed that LOGL8 directly catalyzes the biosynthesis of bioactive cytokinins.Therefore,we propose that the ectopic expression of LOGL8 disrupts cytokinin spatiotemporal distribution and causes inhibition of LONELY GUY(LOG),which affects panicle branching and female organ development.These findings reveal the important role of LOGL8 in male development,and highlight the delicate balance of local cytokinin levels during panicle branching and female organ development.

关键词： Cytokinin-activating enzyme LONELY GUY LOGL8 Pollen Ovule

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Overexpression of maize GOLDEN2 in rice and maize calli improves regeneration by activating chloroplast development

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Science China(Life Sciences) 2023年第2期66卷 340-349页

作者： Wanni Luo Jiantao Tan Tie Li Ziting Feng Zhi Ding Xianrong Xie yuanling chen Letian chen Yao-Guang Liu Qinlong Zhu Jinxing Guo State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources College of Life SciencesSouth China Agricultural UniversitySCAUGuangzhou 510642China Guangdong Laboratory for Lingnan Modern Agriculture Guangzhou 510642China

Golden2(G2), a member of the GARP transcription factor superfamily, regulates several biological processes and phytohormone signaling pathways in plants. In this study, we used a rice codon-optimized maize G2 gene(rZmG2) to improve the regeneration efficiency of rice and maize calli for genetic transformation. We isolated a promoter driving strong and callus-specific expression from rice to drive rZmG2 transcription from a transgene after transformation of two indica and two japonica rice cultivars. The resulting rZmG2 transgenic calli turned green in advance at the differentiation stage, thus significantly raising the regeneration rates of the transgenic indica and japonica rice plants relative to control transformations. Similar effect of this gene on improving maize transformation was also observed. Transcriptome sequencing and RT-qPCR analyses showed that many rice genes related to chloroplast development and phytohormones are upregulated in rZmG2-transgenic calli. These results demonstrate that rZmG2 can promote embryogenic callus differentiation and improve regeneration efficiency by activating chloroplast development and phytohormone pathways. We also established a heat-inducible Cre/loxP-based gene-excision system to remove rZmG2 and the antibiotic selectable gene after obtaining the transgenic plants. This study provides a useful tool for functional genomics work and biotechnology in plants.

关键词： rice ZmG2 genetic transformation callus regeneration chloroplast

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A novel live attenuated vaccine candidate protects chickens against subtype B avian metapneumovirus

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Journal of Integrative Agriculture 2024年第5期23卷 1658-1670页

作者： Lingzhai Meng Mengmeng Yu Suyan Wang Yuntong chen yuanling Bao Peng Liu Xiaoyan Feng Tana He Ru Guo Tao Zhang Mingxue Hu Changjun Liu Xiaole Qi Kai Li Li Gao Yanping Zhang Hongyu Cui Yulong Gao Avian Immunosuppressive Diseases Division State Key Laboratory for Animal Disease Control and PreventionHarbin Veterinary Research InstituteChineseAcademy of Agricultural SciencesHarbin150069China Avian ImmunosuppressiveDiseases DivisionStateKey LaboratoryforAnimal Disease Cont National Poultry Laboratory Animal Resource Center Harbin 150069China

Avian metapneumovirus(aMPV) is a highly contagious pathogen that causes acute upper respiratory tract diseases in chickens and turkeys, resulting in serious economic losses. Subtype B aMPV has recently become the dominant epidemic strain in China. We developed an attenuated aMPV subtype B strain by serial passaging in Vero cells and evaluated its safety and efficacy as a vaccine candidate. The safety test showed that after the 30th passage, the LN16-A strain was fully attenuated, as clinical signs of infection and histological lesions were absent after inoculation.The LN16-A strain did not revert to a virulent strain after five serial passages in chickens. The genomic sequence of LN16-A differed from that of the parent wild-type LN16(wtLN16) strain and had nine amino acid mutations. In chickens, a single immunization with LN16-A induced robust humoral and cellular immune responses, including the abundant production of neutralizing antibodies, CD4^(+) T lymphocytes, and the Th1(IFN-γ) and Th2(IL-4 and IL-6)cytokines. We also confirmed that LN16-A provided 100% protection against subtype B aMPV and significantly reduced viral shedding and turbinate inflammation. Our findings suggest that the LN16-A strain is a promising live attenuated vaccine candidate that can prevent infection with subtype B aMPV.

关键词： avian metapneumovirus attenuated protection vaccine candidate chickens

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Genome-editing technologies: the gap between application and policy

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Science China(Life Sciences) 2019年第11期62卷 1534-1538页

作者： Gousi Li Yao-Guang Liu yuanling chen State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources South China Agricultural UniversityGuangzhou 510642China Key Laboratory of Plant Functional Genomics and Biotechnology of Guangdong Provincial Higher Education Institutions South China Agricultural UniversityGuangzhou 510642China College of Life Sciences South China Agricultural UniversityGuangzhou 510642China

Genome editing is a new technology for manipulating genomic DNA sequences at specific sites,and researchers worldwide are rapidly developing new variants of genomeediting applications that target mRNAs,viruses,chromat... 详细信息

关键词： Genome processes. editing

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双液压马达驱动的甘蔗根切器刀盘转速同步控制方法

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农业工程学报 2024年第11期40卷 59-68页

作者：陈远玲覃良昊陈承宗廖国陆广西大学机械工程学院南宁530004

为简化双刀盘甘蔗根切器的传动链,提高传动系统对时变载荷的适应性,该研究提出用双液压马达直接驱动双刀盘,并将电液比例负载敏感技术应用于根切器的传动方案,通过建模仿真和试验探究双刀盘同步精度的控制方法。建立根切器电液比例阀控... 详细信息

为简化双刀盘甘蔗根切器的传动链,提高传动系统对时变载荷的适应性,该研究提出用双液压马达直接驱动双刀盘,并将电液比例负载敏感技术应用于根切器的传动方案,通过建模仿真和试验探究双刀盘同步精度的控制方法。建立根切器电液比例阀控马达闭环速度控制系统的传递函数,分析得到该系统的稳定裕度仅为16.5°,因此需要采用合适的控制算法提高系统的稳定性和控制精度。基于AMESim-MATLAB联合仿真,研究采用主从控制策略,主马达为PID控制,从马达分别采用PID、自适应模糊PID和滑模变结构控制算法时系统的稳定性和刀盘转速的控制精度。仿真结果为:不同控制算法下马达开启过程转速的动态调整时间分别为5.5、3.0、2.7 s,稳态阶段两个液压马达的速度差分别为20、8、5 r/min;最后搭建试验台,以实测载荷谱为负载输入,进行主从马达转速同步控制试验,得到PID、自适应模糊PID和滑模变结构控制系统启动阶段转速的动态调整时间分别为6.3、4.6、3.7 s,稳态阶段主从马达的转速差分别为47、23、13 r/min;仿真与试验结果均表明,基于滑模变结构算法的刀盘转速同步控制系统的各项指标均优于PID和自适应模糊PID控制。研究结果可为甘蔗收割机根切器传动与控制系统的优化设计提供理论参考。

关键词：农业机械试验甘蔗收获根切器双液压马达同步控制滑模变结构控制

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Rapid Decoding of Sequence-Specific Nuclease- Induced Heterozygous and Biallelic Muta＇dons by Direct Sequencing of PCR Products

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Molecular Plant 2015年第8期8卷 1285-1287页

作者： Xingliang Ma Letian chen Qinlong Zhu yuanling chen Yao-Guang Liu State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources Guangzhou 510642 China College of Life Sciences South China Agricultural University Guangzhou 510642 China Key Laboratory of Plant Functional Genomics and Biotechnology of Guangdong Provincial Higher Education Institutions Guangzhou 510642 China Guangdong Provincial Key Laboratory of Protein Function and Regulation in Agricultural Organisms Guangzhou 510642 China

Dear Editor The recent development of sequence-specific nuclease systems, i.e., TALENs and CRISPR/Casg, has made genomic targeting easier in many organisms including plants （Li et al., 2012; Cong et al., 2013; Joung and Sander, 2013; Li, et al., 2013; Shan et al., 2013; Liang et al., 2014; Zhang et al., 2014）. Mutations induced by CRISPR/Cas9 usually occur around the cleavage sites at three bases upstream of the protospacer-adjacent motif （PAM）, producing insertion and deletion of nucleotides. For diploid organ- isms, such targeted mutations may happen in one or both homol- ogous chromosomes. Previous reports showed that CRISPR/ Cas9-based genomic editing in some plants mainly produced complicated mosaic （chimeric） mutations in the somatic cells of the first generation transgenic plants （Li et al., 2013; Mao et al.,

关键词： SYSTEM ARABIDOPSIS MULTIPLEX PLANTS RICE

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节流槽结构参数对阀开启压力冲击及空化特性的影响研究

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振动与冲击 2024年第7期43卷 50-56,66页

作者：陈远玲欧阳崇钦彭卓陈承宗石浩王梦乔广西大学机械工程学院南宁530004

液压控制阀阀芯节流槽的结构对阀的动态特性影响强烈,不良节流槽结构将导致极大的液压阀开启瞬间压力冲击。针对某型号负载敏感多路阀在甘蔗联合收割机的应用中出现啸叫和液压冲击的问题,结合动网格与RNG-k-ε湍流模型,对U+T型节流槽和... 详细信息

液压控制阀阀芯节流槽的结构对阀的动态特性影响强烈,不良节流槽结构将导致极大的液压阀开启瞬间压力冲击。针对某型号负载敏感多路阀在甘蔗联合收割机的应用中出现啸叫和液压冲击的问题,结合动网格与RNG-k-ε湍流模型,对U+T型节流槽和V型节流槽的压力冲击及空化进行了对比分析,发现同等条件下V型槽的阀芯较U+T型槽的阀芯的压力冲击下降了19.37%,最大空化程度降低了43.7%。选取V型节流槽开展进一步研究,通过正交试验获取不同节流槽结构参数下的压力峰值;采用BP神经网络建立V型节流槽结构参数与压力冲击的代理模型,结合灰狼优化(GWO)算法得到节流槽结构参数的最优配置;依据优化结果制作阀芯并进行试验,结果显示,抑制压力冲击效果显著,系统压力超调下降至原来52.4%。

关键词：流场仿真 BP代理模型灰狼优化(GWO)算法节流槽优化冲击抑制

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新疆单根长绒棉纤维力学特性及断裂形态分析

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纺织学报 2024年第2期45卷 36-44页

作者：马承诺姜开翔陈春晖刘园玲张有强塔里木大学机械电气化工程学院新疆阿拉尔843300 塔里木大学新疆维吾尔自治区教育厅普通高等学校现代农业工程重点实验室新疆阿拉尔843300

针对新疆长绒棉在机采及加工过程中受到机械拉伸导致纤维断裂损伤的问题,首先,通过试验分析纤维直径、试样长度和拉伸速度对单根长绒棉纤维力学特性和形态变化的影响规律;其次,通过响应面法分析并建立3种因素交互作用的响应面回归模型,... 详细信息

针对新疆长绒棉在机采及加工过程中受到机械拉伸导致纤维断裂损伤的问题,首先,通过试验分析纤维直径、试样长度和拉伸速度对单根长绒棉纤维力学特性和形态变化的影响规律;其次,通过响应面法分析并建立3种因素交互作用的响应面回归模型,得到3种因素交互作用对单根长绒棉纤维力学特性的影响规律;最后,借助扫描电子显微镜对不同断裂方式的棉纤维断口形貌进行观察与分析。结果表明:当试样长度和拉伸速度一定时,随着单根长绒棉纤维直径的增大,断裂强力逐渐减小,纤维表面形态变化差异较大;当纤维直径一定时,单根长绒棉纤维断裂强力随着拉伸速度的增大而增大,随着试样长度的增加而减小;单根长绒棉纤维力学特性不仅受上述3种因素的单一影响,受其交互作用的影响也较为显著,影响顺序依次为纤维直径、拉伸速度、试样长度;在低载荷下,单根长绒棉纤维断裂伸长率与拉伸速度没有较大关系,但其随试样长度的增加而增加;新疆单根长绒棉纤维在拉伸过程中力学特性曲线表现为直接断裂和逐渐断裂2种类型,主要原因依赖于棉纤维初始形态和缺陷程度,其拉伸形态变化归纳为纵向微原纤分裂且在纤维内传播,断裂方式具有直接断裂、轴向撕裂、扭转断裂和纤维丝间滑移。

关键词：单根长绒棉纤维力学特性断裂机制断口形貌新疆棉

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The Intronic cis Element SE1 Recruits trans-Acting Repressor Complexes to Repress the Expression of ELONGATED UPPERMOST INTERNODE1 in Rice

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Molecular Plant 2018年第5期11卷 720-735页

作者： Yongyao Xie Yaling Zhang Jingluan Han Jikai Luo Gousi Li Jianle Huang Haibin WU Qingwei Tian Qinlong Zhu yuanling chen Yoji Kawano Yao-Guang Liu Letian chen State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources South China Agricultural University Guangzhou 510642 China Guangdong Provincial Key Laboratory of Protein Function and Regulation in Agricultural Organisms South China Agricultural University Guangzhou 510642 China Key Laboratory of Plant Functional Genomics and Biotechnology of Guangdong Provincial Higher Education Institutions South China Agricultural University Guangzhou 510642 China College of Life Sciences South China Agricultural University Guangzhou 510642 China Shanghai Center for Plant Stress Biology Chinese Academy of Sciences Shanghai 201602 China

Plant height has a major effect on grain yield in crops such as rice （Oryza sativa）, and the hormone gibberellic acid （GA） regulates many developmental processes that feed into plant height. Rice ELONGATED UPPERMOST INTERNODE1 （Euil） encodes a GA-deactivating enzyme governing elongation of the uppermost internode. The expression of Euil is finely tuned, thereby maintaining homeostasis of endogenous bioactive GA and producing plants of normal plant height. Here, we identified a dominant dwarf mutant, dEuil, caused by the deletion of an RY motif-containing cis-silencing element （SE1） in the intron of Euil. Detailed genetic and molecular analysis of SE1 revealed that this intronic cis element recruits at least one trans-acting repressor complex, containing the B3 repressors OsVAL2 and OsGD1, the SAP18 corepressor, and the histone deacetylase OsHDA710, to negatively regulate the expression of Euil. This com- plex generates closed chromatin at Euil, suppressing Euil expression and modulating GA homeostasis. Loss of SE1 or dysfunction of the complex components impairs histone deacetylation and H3K27me3 methylation of Euil chromatin, thereby increasing Euil transcription and decreasing bioactive GA, producing dwarfism in rice. Together, our results reveal a novel silencing mechanism in which the intronic cis element SE1 negatively regulates Euil expression via repressor complexes that modulate histone deacetylation and/or methylation.

关键词： B3 domain Epigenetic regulation Euil Histone Deacetylation H3K27me3 Methylation RY Element

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文化和旅游深度融合发展的系统逻辑架构研究

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学术探索 2024年第2期 126-131页

作者：明庆忠刘安乐祁苑玲晏雄陈刚云南财经大学旅游文化产业研究院云南昆明650221 六盘水师范学院旅游学院贵州六盘水553004 中共云南省委党校云南昆明650504 云南财经大学旅游与酒店管理学院云南财经大学经济研究院云南昆明650221

文章从不断满足人民对美好生活新期待视角、以推进中国式现代化文化和旅游深度融合发展的价值与内在要求为研究起点,分析文化和旅游深度的融合价值与内在要求,提出其系统逻辑框架。文章认为:文化和旅游深度融合的三大逻辑体系可以分解... 详细信息

文章从不断满足人民对美好生活新期待视角、以推进中国式现代化文化和旅游深度融合发展的价值与内在要求为研究起点,分析文化和旅游深度的融合价值与内在要求,提出其系统逻辑框架。文章认为:文化和旅游深度融合的三大逻辑体系可以分解为理论逻辑、发展逻辑和支持逻辑。理论逻辑是揭示文化和旅游深度融合发展多层级、多阶段共演型系统;发展逻辑是探索和创新文化和旅游深度融合的现代产业体系与运行机制;支撑逻辑是强化文化和旅游深度融合的治理体系、保障体系及发展策略。内在机理—现代产业体系及运行—综合治理与保障体系及发展策略是中国式现代化下的文化和旅游深度融合发展系统研究框架。

关键词：文化和旅游深度融合系统思维逻辑中国式现代化

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