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中兽药增强免疫功能的研究

中国家禽 2009年第22期31卷 38-40页

作者：胡元亮南京农业大学江苏南京210095

1中药免疫溯源在西方，“免疫”一词的出现可以追溯到古罗马时代，在中世纪天主教语中的词意思是除罪，使个人的生命和财产免受邪恶的控制。在中国，“免疫”一词最早见于18世纪《免疫类方》一书，意思是免除疫病的危害。

关键词：增强免疫功能中兽药古罗马天主教疫病

Effects of Bacillus subtilis KN-42 on Growth Performance,Diarrhea and Faecal Bacterial Flora of Weaned Piglets

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Effects of Bacillus subtilis KN-42 on Growth Performance,Dia...

2017第二届中国畜牧生物科技大会

作者： yuanliang hu Yaohao Dun Shenao Li Shumiao Zhao Nan Peng Yunxiang Liang State Key Laboratory of Agricultural Microbiology College of Life Science and Technology College of Life Sciences Hubei Normal University

This research focused on the effects of different doses of Bacillus subtilis KN-42 on the growth performance,diarrhea incidence,faecal bacterial flora,and the relative number of Lactobacillus and Escherichia coli in faeces of weaned piglets to determine whether the strain can serve as a candidate antimicrobial growth promoter.A total of 360 piglets(initial BW 7.14±0.63 kg)weaned at 26±2 days of age were randomly allotted to 5 treatment groups(4 pens per treatment with 18 pigs per pen)for a 28-day *** treatments were basal diet without any antimicrobial(negative control;NC),basal diet supplemented with 120 mg/kg feed of neomycin sulfate(positive control;PC)and basal diet supplemented with 2×10(L),4×10(M)and 20×10(H)CFU/kg feed of *** *** the overall period,ADG and feed efficiency of piglets were higher in groups PC,M,and H than those in group NC(P<0.05),and all probiotics and antibiotics groups had a lower diarrhea index than group NC(P<0.05).The 16S rDNA gene-based methods were used to analyze faecal bacterial flora on day 28 of *** result of denaturing gradient gel electrophoresis(DGGE)analysis showed that supplementation of *** KN-42 to the diet changed the bacterial communities,with a higher bacterial diversity and band number in group M than in the other four ***-time PCR analysis showed that the relative number of Lactobacillus were higher in groups PC and H than in group NC(P<0.05),and the supplemented *** KN-42 to the diet also reduced the relative number of ***(P<0.05).These results suggest that dietary addition of *** KN-42 can improve the growth performance and gastrointestinal health of piglets.

关键词： Bacillus subtilis Bacterial community Diarrhea Growth performance Lactobacillus Weaned piglets

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普鲁兰酶的应用对柠檬酸发酵的影响研究

中国酿造 2010年第3期29卷 119-121页

作者：石鹤胡远亮湖北师范学院生命科学学院湖北黄石435002

通过10L发酵罐试验,研究了普鲁兰酶的应用对柠檬酸发酵的影响。研究结果表明:在原料处理中按0.01%加普鲁兰酶,产酸率提高1.1%,转化率提高8.2%,发酵周期缩短12h。

关键词：普鲁兰酶柠檬酸发酵

Dietary Enterococcus faecalis LAB31 Improves Growth Performance,Reduces Diarrhea,and Increases Fecal Lactobacillus Number of Weaned Piglets

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Dietary Enterococcus faecalis LAB31 Improves Growth Performa...

灵芝粉枯草芽孢杆菌发酵条件的优化与质量检测

2017第二届中国畜牧生物科技大会

作者： yuanliang hu Yaohao Dun Shenao Li Dongxiao Zhang Nan Peng Shumiao Zhao Yunxiang Liang State Key Laboratory of Agricultural Microbiology College of Life Science and TechnologyHuazhong Agricultural University College of Life Sciences Hubei Normal University

Lactic acid bacteria(LAB)have been shown to enhance performance of weaned ***,few studies have reported the addition of LAB Enterococcus faecalis as alternatives to growth promoting antibiotics for weaned *** study evaluated the effects of dietary *** LAB31 on the growth performance,diarrhea incidence,blood parameters,fecal bacterial and Lactobacillus communities in weaned piglets.A total of360 piglets weaned at 26±2 days of age were randomly allotted to 5 groups(20 pens,with 4 pens for each group)for a trial of 28 days:group N(negative control,without antibiotics or probiotics):group P(Neomycin sulfate,100mg/kg feed);groups L.M and H(supplemented with *** LAB31 0.5×10,1.0×10,and 2.5×10 CFU/kg feed,respectively).Average daily gain and feed conversion efficiency were found to be higher in group H than in group N,and showed significant differences between group H and group P(P<0.05).Furthermore,groups H and P had a lower diarrhea index than the other three groups(P<0.05).Denaturing gradient gel electrophoresis(DGGE)showed that the application of probiotics to the diet changed the bacterial community,with a higher bacterial diversity in group M than in the other four ***-time *** that the relative number of Lactobacillus increased by addition of probiotics,and was higher in group H than in group N(P<0.05).However,group-specific PCR-DGGE showed no obvious difference among the five groups in Lactobacillus composition and ***,the dietary addition of *** LAB31 can improve growth performance,reduce diarrhea,and increase the relative number of Lactobacillus in feces of weaned piglets.

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畜牧与兽医 2021年第9期53卷 119-122页

作者：徐孝宙胡元亮南京农业大学江苏南京210095 江苏农林职业技术学院江苏句容212400

灵芝是多孔菌科真菌赤芝或紫芝的子实体,含有灵芝多糖等多种可调节动物免疫的活性成分,发酵可提高灵芝中免疫活性物质的吸收与利用,本试验旨在探索优化枯草芽孢杆菌发酵灵芝的条件。将枯草芽孢杆菌菌液接种于粉碎的灵芝,通过考查发酵后... 详细信息

灵芝是多孔菌科真菌赤芝或紫芝的子实体,含有灵芝多糖等多种可调节动物免疫的活性成分,发酵可提高灵芝中免疫活性物质的吸收与利用,本试验旨在探索优化枯草芽孢杆菌发酵灵芝的条件。将枯草芽孢杆菌菌液接种于粉碎的灵芝,通过考查发酵后枯草芽孢杆菌的数量,对接种比例、发酵温度和发酵时间进行优化。结果表明,枯草芽孢杆菌发酵灵芝最佳发酵条件为:接种比例为1∶5,发酵温度为37℃,发酵时间为22 h,此条件下发酵产物中枯草芽孢杆菌数最多,可达1.0×10^(8) CFU/g;检测常温保存30 d的发酵产物不见霉菌生长,枯草芽孢杆菌数量保持1.0×10^(8) CFU/g水平不变。发酵灵芝产品可用于提高动物的免疫抗病力,为畜牧生产中代替抗生素使用提供依据。

关键词：灵芝发酵发酵条件枯草芽孢杆菌

Predicting the strut forces of the steel supporting structure of deep excavation considering various factors by machine learning methods

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Underground Space 2024年第5期18卷 114-129页

作者： Haibo hu Xunjian hu Xiaonan Gong Research Center of Coastal and Urban Geotechnical Engineering Zhejiang UniversityHangzhou 310058China

The application of steel strut force servo systems in deep excavation engineering is not widespread,and there is a notable scarcity of in-situ measured *** presents a significant research gap in the *** this,our study introduces a valuable dataset and application scenarios,serving as a reference point for future *** main objective of this study is to use machine learning(ML)methods for accurately predicting strut forces in steel supporting structures,a crucial aspect for the safety and stability of deep excavation *** employed five different ML methods:radial basis function neural network(RBFNN),back propagation neural network(BPNN),K-Nearest Neighbor(KNN),support vector machine(SVM),and random forest(RF),utilizing a dataset of 2208 measured *** points included one output parameter(strut forces)and seven input parameters(vertical position of strut,plane position of strut,time,temperature,unit weight,cohesion,and internal frictional angle).The effectiveness of these methods was assessed using root mean square error(RMSE),correlation coefficient(R),and mean absolute error(MAE).Our findings indicate that the BPNN method outperforms others,with RMSE,R,and MAE values of 72.1 kN,0.9931,and 57.4 kN,respectively,on the testing *** study underscores the potential of ML methods in precisely predicting strut forces in deep excavation engineering,contributing to enhanced safety measures and project planning.

关键词： Deep excavation Steel supporting structure Strut forces Machine learning Time Temperature

硫酸化银耳多糖和党参多糖对鸡T淋巴细胞增殖及白细胞介素-2mRNA表达水平的影响

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动物营养学报 2017年第7期29卷 2535-2540页

作者：朱广双胡元亮曹侃王本忠芜湖职业技术学院生物工程学院芜湖241003 南京农业大学动物医学院南京210095

为研究硫酸化银耳多糖(sTPS_(70c))和硫酸化党参多糖(sCPPS_(50c))增强免疫作用机理,本试验以未修饰银耳多糖(TPStp)为对照,采用噻唑蓝(MTT)法和实时荧光定量PCR法测定了sTPS_(70c)和sCPPS_(50c)对鸡T淋巴细胞增殖及白细胞介素-2(IL-2)m... 详细信息

为研究硫酸化银耳多糖(sTPS_(70c))和硫酸化党参多糖(sCPPS_(50c))增强免疫作用机理,本试验以未修饰银耳多糖(TPStp)为对照,采用噻唑蓝(MTT)法和实时荧光定量PCR法测定了sTPS_(70c)和sCPPS_(50c)对鸡T淋巴细胞增殖及白细胞介素-2(IL-2)mRNA表达水平的影响。结果表明,多糖单独加入到外周血淋巴细胞时,sTPS_(70c)和sCPPS_(50c)几乎所有浓度均可显著刺激T淋巴细胞的增殖(P<0.05),而TPStp仅在浓度为3.125μg/mL时显著刺激T淋巴细胞增殖(P<0.05);多糖与植物血凝素P(PHA-P)同时加入到外周血淋巴细胞时,sCPPS_(50c)在浓度为0.3911.563μg/mL时显著刺激T淋巴细胞增殖(P<0.05);sTPS_(70c)和sCPPS_(50c)在浓度为1.563μg/mL时可提高T淋巴细胞IL-2 mRNA的表达水平(P<0.05),其中sTPS_(70c)对IL-2 mRNA的促表达作用显著强于TPS_(tp)(P<0.05),且在浓度为1.563μg/mL时的作用最强。结果提示,硫酸化修饰可以提高多糖的淋巴细胞增殖活性及显著增强IL-2 mRNA的表达,且以sTPS_(70c)的作用较强,这与取代度有一定的相关性。

关键词：硫酸化银耳多糖硫酸化党参多糖淋巴细胞增殖白细胞介素-2

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淫蜂佐剂影响动物免疫功能的实验研究

畜牧兽医学报 2000年第2期31卷 155-160页

作者：胡元亮刘家国宋大鲁许志刚南京农业大学动物医学院南京210095

分别以环磷酰胺、福氏佐剂作对照 ,观察淫蜂佐剂对小鼠、雏鸡T淋巴细胞转化率和家兔、母鸡特异性抗体水平的影响 ,结果表明 ,淫蜂佐剂不仅能显著提高 4周龄小鼠和 2 1、35日龄雏鸡的细胞免疫功能 ,有效地拮抗环磷酰胺的免疫抑制 ,并与... 详细信息

分别以环磷酰胺、福氏佐剂作对照 ,观察淫蜂佐剂对小鼠、雏鸡T淋巴细胞转化率和家兔、母鸡特异性抗体水平的影响 ,结果表明 ,淫蜂佐剂不仅能显著提高 4周龄小鼠和 2 1、35日龄雏鸡的细胞免疫功能 ,有效地拮抗环磷酰胺的免疫抑制 ,并与其剂量和动物年龄有相关性 ;而且能显著提高家兔、母鸡的体液免疫功能 ,效果与福氏佐剂类似 ,但比福氏佐剂的副作用小、使用简便。

关键词：淫蜂佐剂动物细胞免疫体液免疫免疫机理

R-matrix for the 8-Dimensional Spin Representation of Uq(so7)

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Acta Mathematica Sinica,English Series 2024年第2期40卷 485-497页

作者： Hong Mei hu Nai Hong hu School of Mathematical Sciences Suzhou University of Science and TechnologySuzhou 215009P.R.China School of Mathematical Sciences MOE Key Laboratory of Mathematics and Engineering Applications&Shanghai Key Laboratory of PMMPEast China Normal UniversityShanghai 200241P.R.China

The purpose of the paper is to describe the solution to the quantum Yang-Baxter equation associated with the 8-dimensional spin representation Tsp of Uq(so7).The self-duality of Tsp and the symmetry of the corresponding braiding matrix are ***,the minimal polynomial of the braiding R-matrix of Tsp is presented explicitly in an ingenious method by taking advantage of nice features of the spin representation Tsp.

关键词： Braiding R-matrix spin module minimal polynomial