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马铃薯StSuSy4基因转录调控因子的筛选及互作验证

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云南农业大学学报（自然科学版） 2024年第3期39卷 17-24页

作者：魏京杨梅宏施豪俞婷黄丹丹迟绍轶高冬丽朱炎辉云南师范大学云南省马铃薯生物学重点实验室云南昆明650500 中国农业科学院深圳农业基因组研究所广东深圳518120

【目的】探究马铃薯块茎高表达基因StSuSy4的转录调控机制。【方法】克隆StSuSy4的启动子,构建pAbAi-StSuSy4pro诱饵载体,并将其转化至酵母细胞Y1HGold中,获得诱饵酵母菌株;利用诱饵载体筛选马铃薯幼嫩块茎组织酵母文库,并对转录因子进... 详细信息

【目的】探究马铃薯块茎高表达基因StSuSy4的转录调控机制。【方法】克隆StSuSy4的启动子,构建pAbAi-StSuSy4pro诱饵载体,并将其转化至酵母细胞Y1HGold中,获得诱饵酵母菌株;利用诱饵载体筛选马铃薯幼嫩块茎组织酵母文库,并对转录因子进行组织表达模式分析。【结果】经酵母单杂交筛选,获得1个候选转录因子StBBX25,该基因的开放阅读框为774 bp,编码258个氨基酸。生物信息分析显示:StBBX25的N端含有B-box蛋白的特征结构域B-box1。候选转录因子与启动子点对点的酵母单杂交验证结果显示:在200 ng/mL金担子素条件下,pGADT7-AD空载对照组酵母不能正常生长,而pGADT7-StBBX25试验组能正常生长,表明StBBX25与StSuSy4基因启动子存在互作。组织表达模式分析显示:StBBX25和StSuSy4均为在马铃薯块茎中表达较高的基因。【结论】StBBX25与StSuSy4启动子互作,暗示StBBX25可能在马铃薯叶片和块茎淀粉合成以及产量形成中发挥作用。研究结果为深入理解马铃薯淀粉合成调控网络提供了重要的理论依据。

关键词：马铃薯淀粉合成 StSuSy4 转录调控酵母单杂交 StBBX25

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Unique fluorophilic pores engineering within porous aromatic frameworks for trace perfluorooctanoic acid removal

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National Science Review 2023年第10期10卷 106-113页

作者： chi Zhang Junchao Dong Panpan Zhang Lei Sun Liu Yang Wenjian Wang Xiaoqin Zou Yunning Chen Qingkun Shang Danyang Feng Guangshan Zhu Faculty of Chemistry Northeast Normal University Institute of Molecular Sciences and Engineering Institute of Frontier and Interdisciplinary Science Shandong University

Perfluorooctanoic acid(PFOA),a representative of per/polyfluorinated alkyl substances,has become a persistent water pollutant of widespread concern due to its biological toxicity and refractory *** this work,we design and synthesize two porous aromatic frameworks(PAF) of PAF-CF3and PAF-C2F5using fluorine-containing alkyl based monomers in tetrahedral *** PAFs exhibit nanosized pores(～1.0 nm) of high surface areas(over 800 m2g-1) and good *** adsorption capacity(>740 mg g-1) and superior efficiency(>24 g mg-1h-1) are achieved toward the removal of PFOA with 1μg L-1concentration owing to unique C-F…F-C *** particular,PAF-CF3and PAF-C2F5are able to reduce the PFOA concentration in water to 37.9 ng L-1and 43.3 ng L-1,below EPA regulations(70 ng L-1).The reusability and high efficiency give both PAFs a great potential for sewage treatment.

关键词： porous aromatic frameworks fluorophilicity perfluorooctanoic acid adsorption removal halogen interactions

Short-term outcome of patients with robot-assisted versus open radical prostatectomy:Prince of Wales Hospital, Hong Kong experience

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Short-term outcome of patients with robot-assisted versus op...

脑脊液白细胞介素-6和白细胞介素-8在儿童细菌性脑膜炎中的临床特征及意义

第十五届全国泌尿外科学术会议

作者： Lo, Ka-Lun Wong, Annie Yip, Sidney ng, chi-Fai Division of Urology, Department of Surgery, The chinese University of Hong Kong 003100

Objective: To perform a retrospective review of the short-term outcome of patients with robotic-assisted (RoRP) versus open radical prostatectomy (ORP). Patients and Method: This was a retrospective review of patients...

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中华实用儿科临床杂志 2024年第8期39卷 584-588页

作者：陈连凤陈文雄朱海霞彭炳蔚侯池曾意茹廖寅婷吴汶霖李小晶广州医科大学附属妇女儿童医疗中心神经内科广州510623

目的探讨儿童细菌性脑膜炎(BM)脑脊液白细胞介素-6(IL-6)和白细胞介素-8(IL-8)的临床特征,为临床诊疗提供参考。方法病例系列研究。收集2019年12月至2022年3月在广州医科大学附属妇女儿童医疗中心住院BM患儿的临床资料,且诊疗过程中脑... 详细信息

目的探讨儿童细菌性脑膜炎(BM)脑脊液白细胞介素-6(IL-6)和白细胞介素-8(IL-8)的临床特征,为临床诊疗提供参考。方法病例系列研究。收集2019年12月至2022年3月在广州医科大学附属妇女儿童医疗中心住院BM患儿的临床资料,且诊疗过程中脑脊液细胞因子送检至少2次。采用t检验、Mann-Whitney检验或方差分析进行统计学分析。结果共纳入40例,年龄2(1,8)个月(2 d至8岁),从起病至广州医科大学附属妇女儿童医疗中心住院时间(15±17)d(1~69 d),起病时主要表现为发热(40例,100%)、精神差(16例,35.0%)、抽搐(9例,22.5%)、呕吐(9例,22.5%)。按致病菌分为无乳链球菌组(GBS组)9例、肺炎链球菌组(SP组)9例、其他菌组9例、未明组13例。BM患儿脑脊液多种细胞因子水平升高,起病第1周内以IL-6、IL-8明显,在第2-3周时达高峰,IL-6、IL-8水平随着病程推移总体上呈下降趋势;10例BM患儿脑脊液IL-6水平在病程约4周时明显降低[2周内773.5(164.1,1781.2)ng/L比4周时10.8(2.2,21.1)ng/L,P=0.005];IL-8水平差异无统计学意义[2周内182.9(33.6,657.7)ng/L比4周时92.9(22.6,226.6)ng/L,P=0.303]。6例患儿经足疗程有效抗菌治疗后在第4-20周时脑脊液白细胞仍偏高,伴或不伴反复间歇性发热,其中4例GBS、1例SP,复查脑脊液病原均阴性,脑脊液IL-6[(149.1~4218.6)ng/L]和IL-8水平[(124.2~1890.3)ng/L]升高;予小剂量糖皮质激素抗炎治疗,其中1例联用丙种球蛋白、1例改用布洛芬。发热完全消退,脑脊液白细胞计数明显下降(P=0.024)。结论脑脊液IL-6和IL-8水平在儿童BM急性期病程早期明显升高,随着病程推移呈下降趋势;如在疾病后期仍明显升高需警惕颅内免疫炎症反应过度可能,尤其致病菌为GBS或SP时。

关键词：细菌性脑膜炎白细胞介素-6 白细胞介素-8 糖皮质激素

Protein trans-splicing based dual-vector delivery of the coagulation factor Ⅷ gene

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中国科学：生命科学英文版 2010年第6期53卷 683-689页

作者： ZHU FuXiang,LIU ZeLong,chi XiaoYan & QU HuiGe Life Science College of Ludong University,Yantai 264025,china Life Science College of Ludong University Yantai China

A dual-vector system was explored for the delivery of the coagulation factor VIII gene,using intein-mediated protein trans-splicing as a means to produce intact functional factor VIII post-translationally.A pair of eukaryotic expression vectors,expressing Ssp DnaB intein-fused heavy and light chain genes of B-domain deleted factor VIII (BDD-FVIII),was *** transient co-transfection of the two vectors into 293 and COS-7 cells,the culture supernatants contained (137±23) and (109±22) ng mL–1 spliced BDD-FVIII antigen with an activity of (1.05±0.16) and (0.79±0.23) IU mL–1 for 293 and COS-7 cells,*** spliced BDD-FVIII was also detected in supernatants from a mixture of cells transfected with inteinfused heavy and light chain *** spliced BDD-FVIII protein bands from cell lysates were visualized by Western *** data demonstrated that intein could be used to transfer the split factor VIII gene and provided valuable information on factor VIII gene delivery by dual-adeno-associated virus in hemophilia A gene therapy.

关键词： intein protein trans-splicing coagulation factor VIII dual-vector gene delivery

动态熔融过程中辉长岩主要矿物的熔融序列及其熔融动力学研究

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岩石学报 2005年第6期21卷 1749-1758页

作者：李化启马瑞梁一鸿迟效国董春艳吉林大学地球科学学院长春130061 吉林大学国土资源部变质动力学开放实验室长春130061

本文以高温高压动态熔融条件下的实验辉长岩样品为研究对象,系统研究了主要矿物的熔融序列和熔融现象,并从熔融动力学的角度对其进行了解释。通过对熔融条件和熔融机制的复杂性和作用机理进行的深入分析,澄清和得出了以下几点重要认识:... 详细信息

本文以高温高压动态熔融条件下的实验辉长岩样品为研究对象,系统研究了主要矿物的熔融序列和熔融现象,并从熔融动力学的角度对其进行了解释。通过对熔融条件和熔融机制的复杂性和作用机理进行的深入分析,澄清和得出了以下几点重要认识:(1)在具体的熔融环境条件下,斜方辉石可以比单斜辉石先熔;(2)熔融阶段、熔融速率不同,矿物的熔融机制也不同,从而导致熔融现象,熔融产物不同;(3)化学因素、或者说元素交代作用,在地质条件下的熔融过程中可能起着重要作用,这和纯物理学上强调的物理熔融有很大不同;(4)一种熔融现象或熔融序列,可能是受多种熔融条件制约的,要进行综合分析对比,才能确定其具体的熔融性质,从而为理解和认识具体的地质过程服务。

关键词：辉长岩动态熔融熔融序列熔融动力学

Cloud Seedability Study with a Dual-Model System

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Atmospheric and Oceanic Science Letters 2013年第4期6卷 197-202页

作者： JIN Ling LEI Heng-chi KOng Fan-You YAng Jie-Fan HU Zhao-Xia Institute of Atmospheric Physics Chinese Academy of Sciences University of chinese Academy of Sciences Center for Analysis and Prediction of Storms University of Oklahoma

In this research, one-dimensional stratiform a novel dual-model system, cold cloud model （1DSC） coupled to Weather Research and Forecast （WRF） model （WRF-1DSC for short）, was employed to investigate the effects of cloud seeding by silver iodide （AgI） on rain enhancement. Driven by changing environmental conditions extracted from the WRF model, WRF-1DSC could be used to assess the cloud seeding effects quantitatively. The employment of WRF- 1DSC, in place of a one-dimen- sional two-moment cloud seeding model applied to a three-dimensional mesoscale cloud-resolving model, was found to result in massive reduction of computational resources. Numerical experiments with WRF-1DSC were conducted for a real stratiform precipitation event ob- served on 4-5 July 2004, in Northeast china. A good agreement between the observed and modeled cloud system ensured the ability of WRF-1DSC to simulate the observed precipitation process efficiently. Sensitivity tests were performed with different seeding times, locations, and amounts. Experimental results showed that the optimum seeding effect （defined as the percentage of rain enhancement or rain enhancement rate） could be achieved through proper seeding at locations of maximum cloud water content when the updraft was strong. The optimum seeding effect was found to increase by 5.61% when the cloud was seeded at 5.5 km above ground level around 2300 UTC 4 July 2004, with the maximum AgI mixing ratio （As） equaling 15 ng kg-1. On the other hand, for an overseeded cloud, a significant reduction occurred in the accumulated precipitation （-12.42%） as Xs reached 100 ng kg^-1. This study demonstrates the potential of WRF- 1DSC in determining the optimal AgI seeding strategy in practical operations of precipitation enhancement.

关键词： dual-model system AgI cloud seeding WRF clmld model

Gastric cancer cell lines induced by trichostatin A

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World Journal of Gastroenterology 2008年第30期14卷 4810-4815页

作者： Xiao-Ming Zou Yun-Long Li Hao Wang Wu Cui Xiao-Lin Li Song-Bin Fu Hong-chi Jiang Department of General Surgery the Second Clinical College Harbin Medical University Harbin 150086 Heilongjiang Province China Laboratory of Medical Genetics Harbin Medical University Harbin 150081 Heilongjiang Province China Department of General Surgery the First Clinical College Harbin Medical University Harbin 150081 Heilongjiang Province China

AIM： To explore the effect of trichostatin A （TSA） on apoptosis and acetylated histone H3 levels in gastric cancer cell lines BGC-823 and SGC-7901. METHODS： The effect of TSA on growth inhibition and apoptosis was examined by MTT, fluorescence microscopy and PI single-labeled flow cytometry. The acetylated histone H3 level was detected by Western blot. RESULTS： TSA induced apoptosis in gastric cancer cell lines BGC-823 and SGC-7901 was in a dose and time-dependent manner. Apoptotic cells varied significantly between TSA treated groups （37.5 ng/mL 72 h for BGC-823 cell line and 75 ng/mL 72 h for SGC-7901 cell line） and control group （0.85 ± 0.14 vs 1.14 ± 0.07, P = 0.02; 0.94 ± 0.07 vs 1.15 ± 0.06, P = 0.02）. Morphologic changes of apoptosis, including nuclear chromatin condensation and fluorescence strength, were observed under fluorescence microscopy. TSA treatment in BGC-823 and SGC-7901 cell lines obviously induced cell apoptosis, which was demonstrated by the increased percentage of sub-G1 phase cells, the reduction of Gl-phase cells and the increase of apoptosis rates in flow cytometric analysis. The result of Western blot showed that the expression of acetylated histone H3 increased in BGC-823 and SGC-7901 TSA treatment groups as compared with the control ***： TSA can induce cell apoptosis in BGC-823 and SGC-7901 cell lines. The expression of acetylated histone H3 might be correlated with apoptosis.

关键词： BGC-823 SGC-7901 Trichostatin A Apoptosis Acetylated histone H3 Gastric cancer

UPLC-MS/MS法测定广叶绣球菌不同生长阶段栽培基质中有机酸含量及其对菌丝生长的影响

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食药用菌 2024年第5期32卷 325-332页

作者：刘晓瑜肖冬来马璐杨驰林辉江晓凌福建省农业科学院食用菌研究所特色食用菌繁育与栽培国家地方联合工程研究中心福建福州350014

为探究绣球菌生长发育过程中有机酸的代谢情况,采用超高效液相色谱串联质谱法(UPLC-MS/MS),以负离子多反应监测(MRM)模式测定广叶绣球菌栽培基质未接种阶段(0 d)、菌丝阶段(40 d)、原基阶段(60 d)及子实体生长阶段(80 d)26种有机酸的含... 详细信息

为探究绣球菌生长发育过程中有机酸的代谢情况,采用超高效液相色谱串联质谱法(UPLC-MS/MS),以负离子多反应监测(MRM)模式测定广叶绣球菌栽培基质未接种阶段(0 d)、菌丝阶段(40 d)、原基阶段(60 d)及子实体生长阶段(80 d)26种有机酸的含量,并研究主要有机酸对菌丝生物量和菌丝生长速度的影响。结果表明:各有机酸在其线性范围内与峰面积呈良好的线性关系(r2≥0.99),检出限在2.0~100.0 ng/mL;有13种有机酸在各阶段均能检测到,其中苹果酸、L-焦谷氨酸、柠檬酸、D-葡萄糖醛酸和酒石酸变化趋势明显,浓度变化分别为113.222~13.826μg/g、41.349~1.418μg/g、16.821~46.399μg/g、15.653~96.850μg/g和4.125~66.550μg/g;酒石酸和L-焦谷氨酸在试验浓度0.02~0.20 g/L范围内均对菌丝生长有显著促进作用,柠檬酸和苹果酸则在添加浓度较高时对菌丝生长促进作用显著。研究有机酸在栽培过程中的变化趋势有利于探究绣球菌基质降解机理,同时也可将其作为安全的营养添加剂用于配方优化。

关键词：广叶绣球菌超高效液相色谱串联质谱法有机酸菌丝生长