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Journal of Integrative Agriculture 2016年第3期15卷 573-579页

作者： XU Cheng-nan ZHAng Hong-jun chi Fu-mei JI Zhi-rui DOng Qing-long CAO Ke-qiang ZHOU Zong-shan Research Institute of Pomology Chinese Academy of Agricultural Sciences College of Plant Protection Agricultural University of Hebei

Botryosphaeriaceae species are important causal agents of blueberry stem blight worldwide. Blueberry stem blight has become an important disease, potentially affecting the quality and production of blueberries in china. It is difficult and time-consuming to identify at the species level using morphological methods. The aim of this study was to develop polymerase chain reaction（PCR） assays for the diagnosis and early detection of latent infections of blueberry stems by Botryosphaeria spp. Species-specific primers, based on the ribosomal DNA internal transcribed spacer region and β-tubulin gene, were designed and selected for use in PCR assays. Three primer pairs, Lt347-F/R for Lasiodiplodia theobromae, Np304-F/R for Neofusicoccum parvum and FaF/Bt2b for Botryosphaeria dothidea, successfully amplified specific PCR fragments of different sizes on pure cultures or from blueberry stems inoculated and naturally infected blueberry plants with three pathogens, respectively. These primers did not amplify any PCR fragments from other blueberry stem disease-associated pathogens, such as Phomopsis spp. and Pestalotiopsis spp. This PCR protocol could detect as low as 1 00 pg to 1 ng of purified fungal DNA. This PCR-based protocol could be used for the diagnosis and detection of these pathogens from pure cultures or from infected blueberry plants.

关键词： blueberry stem blight PCR Botryosphaeriaceae species-specific primer

Cerebrospinal fluid phosphorylated tau,visinin-like protein-1,and chitinase-3-like protein 1 in mild cognitive impairment and Alzheimer’s disease

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Translational Neurodegeneration 2018年第1期7卷 221-232页

作者： Hua Zhang Kok Pin ng Joseph Therriault Min Su Kang Tharick APascoal Pedro Rosa-Neto Serge Gauthier the Alzheimer’s Disease Neuroimaging Initiative Department of Neurology the First Affiliated Hospital of Chongqing Medical UniversityChongqing 400016China Department of Neurology National Neuroscience InstituteSingaporeSingapore The McGill University Research Centre for Studies in Aging McGill UniversityMontrealCanada 不详

Background:Visinin-like protein-1(VILIP-1)and chitinase-3-like protein 1(chi3L1 or YKL-40)in cerebrospinal fluid(CSF)are newly discovered markers indicating neuronal damage and microglial activation,*** tau(p-tau)reflects the neuropathology of Alzheimer’s disease(AD)and is useful as diagnostic markers for ***,it is unknown whether these biomarkers have similar or complementary information in ***:We stratified 121 participants from the Alzheimer’s Disease Neuroimaging Initiative(ADNI)database into cognitively normal(CN),stable mild cognitive impairment(sMCI),progressive MCI(pMCI),and dementia due to *** of covariance(ANOVA)and chi-square analyses,Spearman correlation,and logistic regression models were performed to test the demographic,associations between biomarkers,and diagnostic accuracies,*** mixed-effects models were used to evaluate the effects of CSF amyloid-β(Aβ)on above biomarkers within diagnostic groups,the combination of diagnostic group and Aβstatus as predictor,and CSF biomarkers as predictors of AD features,including cognition measured by Mini–Mental State Examination(MMSE)and brain structure and white matter hyperintensity(WMH)measured by magnetic resonance imaging(MRI).Results:P-tau,VILIP-1,and YKL-40 were all predictors of AD diagnosis,but combinations of biomarkers did not improve the diagnostic accuracy(AUC 0.924 for p-tau,VILIP-1,and YKL-40)compared to p-tau(AUC 0.922).P-tau and VILIP-1 were highly correlated(r=0.639,pngly associated with Aβpathology across clinical stages of AD,while YKL-40 was correlated with Aβpathology in CN and AD ***-1 was associated with acceleration of cognitive decline,hippocampal atrophy,and expansion of ventricles in longitudinal ***-40 was associated with hippocampal atrophy at baseline and follow-up,while p-tau was only associated with worsening WMH at ***:CSF levels of p-tau,VILIP-1,and YKL-40 may have uti

关键词： Alzheimer’s disease Amyloid-β chitinase-3-like protein 1 Phosphorylated tau Visinin-like protein-1

双切口套法预防开放性胃肠手术后切口感染的临床效果观察

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中国当代医药 2015年第21期22卷 35-37页

作者：方赤波广东省惠州市惠阳区中医院外科广东惠州516211

目的探讨双切口套法预防开放性胃肠手术后切口感染的临床效果。方法选取2013年3月-2014年8月在本院实施开放性胃肠手术的75例患者作为研究对象,随机分为观察组（38例）和对照组（37例）。观察组在开放性胃肠手术中采用双切口套法,对照... 详细信息

目的探讨双切口套法预防开放性胃肠手术后切口感染的临床效果。方法选取2013年3月-2014年8月在本院实施开放性胃肠手术的75例患者作为研究对象,随机分为观察组（38例）和对照组（37例）。观察组在开放性胃肠手术中采用双切口套法,对照组术中不使用任何切口保护套。比较两组的手术时间、术后首次排气时间、首次排便时间、住院时间及切口感染率。结果两组的手术时间比较,差异无统计学意义（P〉0.05）。观察组的术后首次排气时间、首次排便时间及住院时间显著短于对照组,差异有统计学意义（P〈0.05）。观察组的切口感染率为2.63%,显著低于对照组的16.22%,差异有统计学意义（P〈0.05）。结论双切口套法可以明显降低开放性胃肠手术后的切口感染率,提高临床疗效。

关键词：双切口套法开放性胃肠手术切口感染

Adaptive cytoprotection through modulation of nitric oxide in ethanol-evoked gastritis

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World Journal of Gastroenterology 2004年第17期10卷 2503-2508页

作者： JoshuaKa-ShunKo chi-HinCho Shiu-KumLam SchoolofchineseMedicine HongKongBaptistUniversityHongKongChina DepartmentofPharmacology FacultyofMedicineTheUniversityofHongKongHongKongChina DepartmentofMedicine FacultyofMedicineTheUniversityofHongKongHongKongChina

AIM: To assess the mechanisms of protective action by different mild irritants through maintenance of gastric mucosal integrity and modulation of mucosal nitric oxide (NO) in experimental gastritis ***: Etcher 200 mL/L ethanol, 50 g/L NaG or 0.3 mol/L HCl was pretreated to normal or 800 mL/L ethanol-induced acute gastritis Sprague-Dawley rats before a subsequent challenge with 500 mL/L ethanol. Both macroscopic lesion areas and histological damage scores were determined in the gastric mucosa of each group of animals. Besides,gastric mucosal activities of NO synthase isoforms and of superoxide dismutase, along with mucosal level of leukotriene (LT)C4 were ***: Macroscopic mucosal damages were protected by 200 mL/L ethanol and 50 g/L NaCI in gastritis ***, although 200 mL/L ethanol could protect the surface layers of mucosal cells in normal animals (protection attenuated by ng-nitro-L-arginine methyl ester), no cytoprotection against deeper histological damages was found in gastritis rats. Besides, inducible NO synthase activity was increased in the mucosa of gastritis animals and unaltered by mild irritants. Nevertheless, the elevation in mucosal LTC4 level following 500 mL/L ethanol administration and under gastritis condition was significantly reduced by pretreatment of all three mild irritants in both normal and gastritis ***: These findings suggest that the aggravated 500 mL/L ethanol-evoked mucosal damages under gastritis condition could be due to increased inducible NO and LTC4 production in the gastric mucosa. Only 200 mL/L ethanol is truly 'cytoprotective' at the surface glandular level of nongastritis mucosa. Furthermore, the macroscopic protection of the three mild irritants involves reduction of LTC4 level in both normal and gastritis mucosa, implicating preservation of the vasculature.

关键词：细胞保护适应性调治作用含氮氧化物酒精性胃炎消化系统 LT 歧化酶

Novel SNP markers on ginsenosides biosynthesis functional gene for authentication of ginseng herbs and commercial products

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chinese Journal of Natural Medicines 2020年第10期18卷 770-778页

作者： WU Wen-Ru CHEng Chun-Song CHEng Qi-Qing LAO chi-Chou CUI Hao TAng Zi-Yu OUYAng Yue LIU Liang ZHOU Hua State Key Laboratory of Quality Research in chinese Medicine Faculty of Chinese MedicineMacao University o f Science and TechnologyMacao 999078China School of chinese Pharmaceutical Sciences Guangzhou University of Chinese MedicineGuangzhou 510006China Joint Laboratory for Translational Cancer Research of chinese Medicine of the Ministry of Education of the People's Republic of china Macao University of Science and TechnologyMacao 999078China

Panax ginseng and Panax quinquefolius have similar bioactive components and morphological characteristics, but they are known to have different medicinal values, high-sensitive and accurate method is expected to identify the sources of ginseng products and evaluate the quality, but with a huge challenge. Our established UHPLC-TOF/MS method coupled with orthogonal partial least squares discriminant analysis(OPLS-DA) model based on 18 ginsenosides was applied to discriminate the sources of raw medicinal materials in ginseng products, and nested PCR strategy was used to discover 6 novel single nucleotide polymorphism(SNP)sites in functional dammarenediol synthase(DS) gene for genetic authentication of P. ginseng and P. quinquefolius for the first ***-DA model could identify the sources of raw ginseng materials are real or not. SNP markers were applied to identify ginseng fresh samples as well as commercial products, and proved to be successful. This established molecular method can tell exact source information of adulterants, and it was highly sensitive and specific even when total DNA amount was only 0.1 ng and the adulteration was as low as 1%. Therefore, this study made an attempt at the exploration of new type SNP marker for variety authentication and function regulation at the same time, and the combination of chemical and molecular discrimination methods provided the comprehensive evaluation and authentication for the sources of ginseng herbs and products.

关键词： Panax ginseng Panax quinquefolius UHPLC-TOF/MS Dammarenediol synthase Nested PCR SNP

Phthalocyanine-Based Theranostic Agents for Cancer Imaging and Therapy

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Phthalocyanine-Based Theranostic Agents for Cancer Imaging a...

Effect of Letrozole,Berberine,or Their Combination for Infertility in Women with Polycystic Ovary Syndrome:Statistical Analysis Plan for a Multicenter Randomized Controlled Trial

中国化学会第五届卟啉与酞菁学术研讨会

作者： Roy C.H.Wong Sun Y.S.Chow Summer Y.Y.Ha Pui-chi Lo Dennis K.P.ng Department of Chemistry The Chinese University of Hong Kong Department of Biomedical Sciences City University of Hong Kong

Photodynamic therapy（PDT） has emerged as a clinical modality for treatment of certain localized and superficial cancers [1].It requires a photosensitive drug,light of specific wavelength,and molecular oxygen to induce a series of photochemical reactions,leading to the formation of cytotoxic reactive oxygen species（ROS）.These ROS destroy tumors by multifactorial mechanisms,including killing malignant cells by apoptosis and/or necrosis,shutting down the tumor vasculature,and stimulating the host immune system [2].With a view to improving the therapeutic outcome,various strategies have been explored to enhance the ROS generation efficiency and tumor selectivity of the *** address the latter aspect,conjugation to tumor-specific vehicles [3] and encapsulation in colloidal nanocarriers [4] are two common ***,"smart" photosensitizing systems that can be activated by tumor-associated stimuli have received considerable attention [5],which can overcome the drawback of nonspecific photodamage in *** this presentation,we will showcase our endeavor along these strategies in advancing phthalocyanine-based theranostic agents for cancer imaging and *** molecular design,synthesis,photophysical properties,as well as in vitro and in vivo biological response of these systems will be reported and discussed.

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World Journal of Traditional chinese Medicine 2016年第3期2卷 36-42页

作者： Hong-Li Ma Jing-Shu Gao Feng Tian Ernest HY ng Xiao-Ke Wu Jian-Ping Liu Centre for Evidence-Based chinese Medicine Beijing University of Chinese Medicine Department of Obstetrics and Gynecology First Affiliated HospitalHeilongjiang University of Chinese Medicine Institute of Basic Research in Clinical Medicine China Academy of Chinese Medical Sciences Department of Obstetrics and Gynaecology The University of Hong KongQueen Mary Hospital

Introduction:Letrozole showed higher ovulation and live birth rates than clomiphene in infertile women with polycystic ovary syndrome(PCOS).Berberine,a major active component of chinese herbal medicine rhizomacoptidis,has been used to improve insulin resistance to facilitate ovulation induction in women with PCOS,but there is no study reporting the live birth or its potential as a complementary treatment to *** aim to determine the efficacy of letrozole with or without berberine in achieving live births among 644 infertile women with PCOS in china's *** and analysis:This is a prospective,randomized,multicentre,double-blinded,controlled *** women with PCOS were randomized into three-arm,letrozole and berberine,letrozole and berberine placebo,letrozole placebo and *** and blood were collected at baseline,the third month and sixth month after treatment,or immediately were collected if subject was *** and clinical investigators were blinded to treatment allocation and treatment related study results until the central database was locked for final data extraction and analysis *** statistical analysis plan described basic analysis principles,methods commonly encountered in data analysis issues,and the specific statistical procedures for analyzing the primary,secondary,and safety *** and dissemination:The study was approved by the ethics committee of the First Affiliated Hospital,Heilongjiang University of chinese *** study findings will be disseminated through peer-reviewed publications and conference *** registration:chinese Clinical Trial Registry:chi *** *** identifier:NCT01116167.

关键词： Polycystic ovary syndrome Statistical analysis plan Randomized controlled trial Letrozole Berberine

血清降钙素在甲状腺髓样癌的诊断、术前评估及术后随访中的临床价值

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中国耳鼻咽喉头颈外科 2024年第1期31卷 7-12页

作者：李婷婷马丙娥黄驰徐书杭武心萍王建华南京中医药大学附属中西医结合医院甲乳外科江苏南京210028 南京中医药大学附属中西医结合医院内分泌科江苏南京210028 南京中医药大学附属中西医结合医院超声科江苏南京210028

目的探讨血清降钙素(calcitonin,Ctn)对甲状腺髓样癌(medullary thyroid carcinoma,MTC)的诊断效能,术前Ctn与临床病理特征相关性及随访中影响MTC疾病进展的危险因素。方法系统性回顾2011年11月~2022年11月南京中医药大学附属中西医结... 详细信息

目的探讨血清降钙素(calcitonin,Ctn)对甲状腺髓样癌(medullary thyroid carcinoma,MTC)的诊断效能,术前Ctn与临床病理特征相关性及随访中影响MTC疾病进展的危险因素。方法系统性回顾2011年11月~2022年11月南京中医药大学附属中西医结合医院收治的50例甲状腺结节患者临床资料,ROC曲线计算Ctn、癌胚抗原(carcinoembryonic antigen,CEA)水平对MTC的诊断效能,单因素、多因素分析MTC中央区淋巴结转移的危险因素,绘制无疾病进展生存曲线预测危险因素。结果ROC曲线结果显示诊断MTC的术前Ctn截断值为23.81 pg/ml,CEA截断值为3.035 ng/ml。MTC患者的患病年龄、肿瘤直径、术前血清Ctn和CEA水平均高于非MTC患者。Ctn≥289.62 pg/ml是MTC患者中央区淋巴结转移的独立危险因素。生存曲线结果显示侵犯包膜、中央区淋巴结转移、T2级以上是预测疾病进展的危险因素(P<0.05),疾病发生进展的MTC患者术前Ctn较高。结论血清Ctn在MTC鉴别诊断、术前评估以及术后随访均有重要临床价值。

关键词：甲状腺肿瘤降钙素癌胚抗原诊断甲状腺髓样癌淋巴结转移

Epidemiological Characteristics of Influenza A and B in Macau,2010–2018

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Virologica Sinica 2021年第5期36卷 1144-1153页

作者： Hoi Man ng Teng Zhang Guoliang Wang Si Meng Kan Guoyi Ma Zhe Li Chang Chen Dandan Wang Meng In Wong chio Hang Wong Jinliang Ni Xiaohua Douglas Zhang Clinical Laboratory Kiang Wu Hospital Faculty of Health Sciences University of Macau

Influenza is one of the major respiratory diseases in humans. Macau is a tourist city with high density of population and special population mobility. The study on the epidemiological characteristics of influenza in Macau should bring great value for preventing influenza in tourist cities like Macau in the world. In this study, we collected a total of 104,874 samples with influenza-like illness(ILI) in Macau from 2010 to 2018. chi-square test and binary multivariable logistic regression were used to investigate the epidemiological characteristics of influenza A and B in Macau. Among these ILI samples, the overall positive rate is 17.17% for influenza A and 6.97% for influenza B. The epidemics of influenza in three years(i.e., 2012, 2017 and 2018) differ from the remaining years(i.e., normal years). In a normal year, influenza A occurs year-round whereas influenza B is seasonal. Our research shows significant differences in influenza infections between different age groups in normal years. Interestingly, our analysis shows no significant difference between locals and tourists in influenza A and B infection in a normal year, whereas the odds of influenza A in tourists were significantly higher than those in locals in July 2017 and the odds of influenza B in tourists were significantly higher than those in locals in January–February 2012 and January–February 2018. This is possibly attributed by the policy of free vaccination to everyone in Macau. These findings should be valuable for preventing influenza in not only Macau but also the world.

关键词： Influenza Epidemiology chi-square test Logistic regression Macau

白细胞介素⁃15预处理调控脓毒症诱导的心肌细胞凋亡及其相关机制研究

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海军医学杂志 2024年第7期45卷 683-689页

作者：李惠熊翅李浪郭艳丹张媛王玉梅许文平中国人民解放军南部战区总医院健康医学科广东广州510010

目的研究白细胞介素(IL)⁃15对脂多糖(LPS)诱导的脓毒症心肌细胞凋亡的影响,并探讨其相关的作用机制。方法H9C2细胞分为对照组(常规培养的H9C2细胞)、凋亡组(建立LPS诱导的心肌细胞损伤模型,未进行其他干预)和干预组(采用10 ng/ml重组大... 详细信息

目的研究白细胞介素(IL)⁃15对脂多糖(LPS)诱导的脓毒症心肌细胞凋亡的影响,并探讨其相关的作用机制。方法H9C2细胞分为对照组(常规培养的H9C2细胞)、凋亡组(建立LPS诱导的心肌细胞损伤模型,未进行其他干预)和干预组(采用10 ng/ml重组大鼠IL⁃15干预心肌H9C2细胞损伤模型6 h)。甲基噻唑基四唑(MTT)法和流式细胞仪检测IL⁃15干预对H9C2细胞增殖和凋亡的影响,四甲基罗丹明乙酯(TMRE)染色测定线粒体膜电位。10 mg/kg LPS腹腔注射建立小鼠脓毒症模型,采用腹腔注射含100μg/ml IL⁃15的生理盐水100μl进行干预。通过HE染色评估小鼠心肌病理损伤。ELISA法检测小鼠心肌组织氧化应激指标。实时荧光定量PCR和蛋白质印迹法检测小鼠心肌组织中血红素氧合酶(HO)⁃1、核转录相关因子2(Nrf2)mRNA和蛋白的表达。结果凋亡组H9C2细胞凋亡率[(47.9±5.1)%]明显高于对照组[(2.1±0.3)%],差异有统计学意义(P<0.05),而干预组H9C2细胞凋亡率[(20.7±2.7)%]则明显低于凋亡组(P<0.05)。LPS诱导可以显著抑制心肌细胞的增殖活性,IL⁃15能够抑制LPS诱导的心肌细胞活力降低(P<0.05)。凋亡组H9C2细胞线粒体膜电位相对值(2.6±4.6)明显低于对照组H9C2细胞线粒体膜电位相对值(7.4±5.2),差异有统计学意义(P<0.05);干预组H9C2细胞线粒体膜电位相对值(4.2±4.9)明显高于凋亡组(P<0.05)。LPS作用后显著增强了H9C2细胞线粒体膜电位的丧失,IL⁃15干预会减少LPS诱导的H9C2细胞线粒体膜电位的丧失。HE染色切片镜下观察显示小鼠脓毒症模型心肌组织出现明显损伤及炎症细胞浸润;IL⁃15干预可改善LPS诱导的小鼠心肌损伤。脓毒症模型小鼠心肌组织中超氧化物歧化酶(SOD)和总抗氧化能力(T⁃AOC)水平较正常小鼠明显降低,而丙二醛(MDA)水平则明显升高(P<0.05)。IL⁃15干预小鼠心肌组织SOD、T⁃AOC水平均升高,MDA水平均降低(P<0.05)。正常小鼠心肌组织中HO⁃1和Nrf2的mRNA和蛋白表达水平均较低;脓毒症模型小鼠心肌组织中HO⁃1、Nrf2的mRNA和蛋白表达水平较正常小鼠明显升高(P<0.05);IL⁃15干预小鼠心肌组织中HO⁃1、Nrf2的mRNA和蛋白表达水平进一步升高(P<0.05)。结论IL⁃15可减轻脓毒症诱导的心肌细胞凋亡,改善脓毒症小鼠心肌损伤程度。其机制可能与IL⁃15激活HO⁃1/Nrf2信号通路,进而抑制脓毒症心肌组织氧化应激有关。

关键词：白细胞介素⁃15 脓毒症心肌细胞凋亡氧化应激