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建设项目土地征收社会稳定风险识别与评估

科技和产业 2024年第14期24卷 181-185页

作者：黄源华于冬升贺州学院建筑与电气工程学院广西贺州542899

基于文献研究与专家访谈识别建设项目土地征收社会稳定风险因素22项,将社会稳定风险因素按合法性、合理性、可行性、可控性进行分类,构建了建设项目土地征收社会风险因素清单。采用评估实例对风险因素清单加以验证,评估结果显示,拟征地... 详细信息

基于文献研究与专家访谈识别建设项目土地征收社会稳定风险因素22项,将社会稳定风险因素按合法性、合理性、可行性、可控性进行分类,构建了建设项目土地征收社会风险因素清单。采用评估实例对风险因素清单加以验证,评估结果显示,拟征地项目的组合风险指数为0.24,属于低社会稳定风险。

关键词：建设项目土地征收社会稳定风险识别

基于网络药理学和分子对接探讨黄芪治疗早发性卵巢功能不全的机制研究

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海南医学院学报 2024年第15期30卷 1156-1168页

作者：李锦婧钟婉黄元华马燕琳海南医学院第一附属医院海南省人类生殖与遗传重点实验室海南医学院生殖健康及相关疾病研究与转化教育部重点实验室海南医学院第一附属医院生殖医学科海南医学院第一附属医院海南省地方病(地中海贫血)临床医学研究中心国家国际科技合作基地“中‑缅区域性重大疾病防治联合研究中心”海南医学院第一附属医院海口市人类遗传资源保藏重点实验室海南海口571199 海南医学院口腔医学院海南海口571199

目的:基于网络药理学和分子对接方法探讨黄芪对早发性卵巢功能不全治疗作用的分子机制。方法:在中药系统药理学数据库TCMSP中筛选黄芪有效成分及药物作用靶点;在Genecards数据库中筛选早发性卵巢功能不全疾病靶点;运用Venny在线工具筛... 详细信息

目的:基于网络药理学和分子对接方法探讨黄芪对早发性卵巢功能不全治疗作用的分子机制。方法:在中药系统药理学数据库TCMSP中筛选黄芪有效成分及药物作用靶点;在Genecards数据库中筛选早发性卵巢功能不全疾病靶点;运用Venny在线工具筛选两者的交互基因;通过Cytoscape3.7.2软件构建“药物-成分-靶点-疾病”网络关系图;采用String数据库获得蛋白互作(PPI)信息,进一步通过CytoNCA插件构建蛋白质相互作用网络,筛核心靶点蛋白;利用DAVID数据库对交互基因进行GO富集和KEGG通路富集;使用分子对接技术进一步明确其发挥治疗作用的分子;最后,采用血清饥饿法建立POI细胞模型,给予黄芪中的有效化合物槲皮素进行干预,通过Tunel染色、蛋白质印迹实验(western blotting,WB)对网络药理学预测结果进行实验验证。结果:通过TCMSP数据库筛选出黄芪的32个有效化合物及552个基因靶点,从疾病数据库中筛选出730个早发性卵巢功能不全的疾病靶点,两者共62个交集基因,其中黄芪中的主要活性成分包括槲皮素(quercetin)、异鼠李素(isorhamnetin)、山奈酚(kaempferol)、丁子香萜(Mairin)、芒柄花黄素(formononetin)和芒柄花黄素(astragaloside IV),10个POI的潜在核心靶基因为ALB、INS、AKT1、ACTB、TP53、TNF、ESR1、CASP3、STAT3和PTEN;潜在靶点基因主要参与调控JAK2/STAT3、PI3K/Akt信号通路;分子对接验证了黄芪主要活性化合物与核心蛋白STAT3和Akt1存在较强的结合作用;细胞实验结果发现,黄芪中的重要活性化合物槲皮素能促进JAK2/STAT3信号通路关键分子JAK2及STAT3的磷酸化水平,改变卵巢颗粒细胞凋亡相关蛋白表达水平。结论:网络药理学和分子对接分析表明中药黄芪能通过调节对雌二醇的反应、抑制凋亡过程以及调控JAK-STAT和PI3K-Akt信号通路治疗早发性卵巢功能不全,其机制可能与激活JAK2/STAT3信号通路,抑制卵巢颗粒细胞凋亡相关。

关键词：网络药理学分子对接黄芪早发性卵巢功能不全信号通路细胞凋亡

Application of next-generation sequencing in thalassemia screening:A systematic review and meta-analysis

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Asian Pacific Journal of Tropical Medicine 2023年第2期16卷 51-57,I0001,I0002页

作者： Xingyi Fang Yi Gong Yanlin Ma yuanhua huang Hainan Provincial Key Laboratory for Human Reproductive Medicine and Genetic Research Reproductive Medical CenterInternational Technology Cooperation Base"China-Myanmar Joint Research Center for Prevention and Treatment of Regional Major Disease"by the Ministry of Science and Technology of Chinathe First Affiliated Hospital of Hainan Medical UniversityHainan Medical UniversityHaikou 570102China Key Laboratory of Tropical Translational Medicine of Ministry of Education Hainan Medical UniversityHaikou 570102China Hainan Provincial Clinical Research Center for Thalassemia the First Affiliated Hospital of Hainan Medical UniversityHainan Medical UniversityHaikou 570102China Department of Obstetrics and Gynecology Southern Medical University Nanfang HospitalGuangzhou 510515China

Objective:To evaluate the value of next-generation sequencing(NGS)in the prevention and management of ***:A systematic search was performed in eight databases including China Biomedical Literature Database,Chinese National Knowledge Infrastructure,Chinese Scientific Journals Database,Wanfang database,PubMed,EMBASE,Web of Science,and Cochrane Library from the inception to 1 June *** 17.0 and Review Manager 5.4 were used for the ***:Nine studies containing 14794 participants were included in the *** with the routine genetic testing(including Gap-PCR and reverse dot blot),NGS had higher detection rates in screening thalassemia(RR 1.22,95%CI 1.13-1.31,P0.05).Conclusions:Compared with routine genetic testing,NGS had a higher detection rate in general,particularly in the detection ofα-thalassemia.

关键词： Thalassaemia Next-generation sequencing Meta-analysis

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合金钛粉制备铝合金钛剂的研究及应用

云南冶金 2024年第3期53卷 258-262页

作者：邹学通陈碧武刘金志钟继武黄元桦阳建云南云铝涌鑫铝业有限公司云南建水654300

针对铝合金钛添加剂实收率波动的问题,研究了合金钛粉的粒度、种类、钛剂压制工艺及钛剂熔化工艺对钛添加剂的熔解速度和实收率的影响,选取了以合金钛粉为主,氢化脱氢钛粉+海绵钛粉+助熔剂为辅的配方,通过试验在合金钛粉粒度0.42~0.59 ... 详细信息

针对铝合金钛添加剂实收率波动的问题,研究了合金钛粉的粒度、种类、钛剂压制工艺及钛剂熔化工艺对钛添加剂的熔解速度和实收率的影响,选取了以合金钛粉为主,氢化脱氢钛粉+海绵钛粉+助熔剂为辅的配方,通过试验在合金钛粉粒度0.42~0.59 mm,液压机压强18~24 MPa,钛剂压块密度2.9~3.1 g/cm^(3)的条件下,钛元素的实收率为95%。

关键词：合金钛粉铝合金用钛剂钛剂制备技术熔钛工艺钛实收率

Organoid-based single-cell spatiotemporal gene expression landscape of human embryonic development and hematopoiesis

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Signal Transduction and Targeted Therapy 2023年第7期8卷 3218-3220页

作者： Yiming Chao Yang Xiang Jiashun Xiao Weizhong Zheng Mo R.Ebrahimkhani Can Yang Angela Ruohao Wu Pentao Liu yuanhua huang Ryohichi Sugimura The University of Hong Kong Hong KongChina Centre for Translational Stem Cell Biology Hong KongChina The Hong Kong University of Science and Technology Hong KongChina University of Pittsburgh PittsburghPAUSA

Dear Editor,The cell-cell interactions are fundamental mechanisms of differentiating embryonic tissues.1 We aimed to model human embryonic development in stem-cell-derived *** exploited the time series single-cell and spatial transcriptomics to reveal the gene expression landscape in the development of embryonic tissues and their interactions.

关键词： landscape cell development

Class Ⅰ integron with a novel cassette array in an ESBL-producing multidrug-resistant Klebsiella pneumoniae isolate

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Journal of Nanjing Medical University 2006年第1期20卷 12-16页

作者： Bing Gu Mingqing Tong Wangsheng Zhao Shiyang Pan yuanhua Wei Peijun huang Department of Clinical Laboratories the First Affiliated Hospital of Nanjing Medical University Nanjing 210029 China

Objective： To analyze the molecular mechanism of integron mediated mulfi-resistanc, e in an ESBL-producing K. pneumoniae NJ 12 isolate. Methods： Susceptibility test was carried out by Kirby-Bauer method. Class Ⅰ, Ⅱ and Ⅲ integrons were detected by integrase gene PCR with primers that annealed to conserved regions of integron-encoded integrase genes intll, intl2 and intl3. The variable region of integron was amplified by integron PCR with primers that targeted the conserved flanking regions, and the product was sequenced. Six aminoglycoside modifying-enzyme genes, including ant（2＂）-Ⅰ, ant（3＂）- Ⅰ, aac（3）- Ⅰ, aac（3）-Ⅱ, aac （6＇）-Ⅰ, and aac（6＇）-Ⅱ , were detected. Results： K. pneumoniae NJ 12 was resistant to nine antibiotics, including piperacillin, ampicillin, cefuroxime, ceftazidime, cefotaxime, aztreonam, streptomycin, gentamicin and amikacin. This isolate was shown that there was positive with class Ⅰ integron, ant（2＂）- Ⅰ , ant（3＂）- Ⅰ , aac（3）-Ⅱ and aac（6＇）- Ⅰ modifying-enzyme genes. Neither class Ⅱ nor Ⅲ integron was detected; DNA sequencing of the fragment amplified by integron PCR revealed a novel cassette array aadR-cat-blaoxa-10/ aadA1. Conclusion： Class I integron with a novel cassette array in an ESBL-producing multidrug-resistant K. pneumon/ae NJ 12 isolate was reported from Nanjing area of China, with the GenBank accession number DQ141319.

关键词： integron gene cassette multi-resistance K.pneumoniae ESBL

Non-flammable long chain phosphate ester based electrolyte via competitive solventized structures for high-performance lithium metal batteries

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Journal of Energy Chemistry 2024年第10期97卷 156-165,I0004页

作者： Li Liao Zhiqiang Han Xuanjie Feng Pan Luo Jialin Song Yin Shen Xiaoshuang Luo Xinpeng Li Xuanzhong Wen Bo Yu Junchen Chen Bingshu Guo Mingshan Wang Yun huang Hongmei Zhang Mengmeng Yin Jiangtao Liu yuanhua Lin Xing Li School of New Energy and Materials Southwest Petroleum UniversityChengdu 610500SichuanChina State Key Laboratory of Advanced Chemical Power Sources Guizhou Meiling Power Sources Co.LtdZunyi 563003GuizhouChina Beijing Advanced Innovation Center for Materials Genome Engineering Center for Green InnovationSchool of Mathematics and PhysicsUniversity of Science and Technology BeijingBeijing 100083China

Safety remains a persistent challenge for high-energy-density lithium metal batteries(LMBs).The development of safe and non-flammable electrolytes is especially important in harsh conditions such as high ***,a flame-retardant,low-cost and thermally stable long chain phosphate ester based(tributyl phosphate,TBP)electrolyte is reported,which can effectively enhance the cycling stability of highly loaded high-nickel LMBs with high safety through co-solvation *** interfacial compatibility between TBP and electrode is effectively improved using a short-chain ether(glycol dimethyl ether,DME),and a specially competitive solvation structure is further constructed using lithium borate difluorooxalate(LiDFOB)to form the stable and inorganic-rich electrode *** from the presence of the cathode electrolyte interphase(CEI)and solid electrolyte interphase(SEI)enriched with LiF and Li_(x)PO_(y)F_(z),the electrolyte demonstrates excellent cycling stability assembled using a 50μm lithium foil anode in combination with a high loading NMC811(15.4 mg cm^(-2))cathode,with 88%capacity retention after 120 ***,the electrolyte exhibits excellent high-temperature characteristics when used in a 1-Ah pouch cell(N/P=0.26),and higher thermal runaway temperature(238℃)in the ARC(accelerating rate calorimeter)demonstrating high *** novel electrolyte adopts long-chain phosphate as the main solvent for the first time,and would provide a new idea for the development of extremely high safety and high-temperature electrolytes.

关键词： Non-flammable electrolyte Long chain phosphate ester Solvation structure Lithium metal batteries Battery safety

SLAMF8 as a potential biomarker for rheumatoid arthritis identified by comparing peripheral blood mononuclear cells,fibroblast-like synoviocytes,and synovial tissue using bioinformatics analysis

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Rheumatology & Autoimmunity 2024年第2期4卷 99-108页

作者： Cheng Zhang Huina huang Jie Zhang Yifan huang yuanhua Qin Xia Li Guan Wang Department of Immunology College of Basic Medical ScienceDalian Medical UniversityDalianLiaoningChina Department of Orthopedics Zhengzhou University People's HospitalHenan Provincial People's HospitalZhengzhouHenanChina Department of Parasite College of Basic Medical SciencesDalian Medical UniversityDalianLiaoningChina

Background::Rheumatoid arthritis(RA)is a chronic autoimmune inflammatory *** pathogenesis is not fully understood,and early diagnosis is challenging owing to the lack of effective *** study aimed to analyze different samples to identify potential biomarkers and therapeutic ***::Microarray datasets of RA,osteoarthritis(OA),and healthy control(HC)were downloaded from the Gene Expression Omnibus database.R software was used to identify differentially expressed genes(DEGs),which were visualized using volcano and heat *** diagrams,principal component analysis,gene set enrichment analysis,gene ontology,and Kyoto Encyclopedia of genes and genomes were used to analyze the data.A protein–protein interaction network was constructed,and synovial tissues from patients with RA and OA were collected for verification using the collagen-induced arthritis mouse ***::More DEGs were found in synovial tissues than in peripheral blood mononuclear cells or fibroblast-like *** component analysis revealed significant differences between the RA and OA samples,highlighting the unique advantages of synovial *** analysis revealed that metabolic and cytokine signaling pathways play crucial roles in the development of *** analysis of the four synovial datasets identified 54 DEGs,of which signaling lymphocytic activation molecule family(SLAMF)8 was identified as the key ***8 levels were increased in the synovial tissue of patients with RA compared to those of patients with OA(0.38±0.19 vs.12.40±1.66),and SLAMF8 levels were similarly elevated in collagen-induced arthritis model mice compared with those in the healthy mice(1.13±0.47 vs.9.05±2.52).Conclusions::This study established the unique advantages of synovial tissue for RA research and identified metabolic and cytokine signaling pathways as important for RA ***,SLAMF8 may be a potential therapeutic target for RA.

关键词： bioinformatics analysis rheumatoid arthritis SLAMF8 synovial tissue

基于改进哈里斯鹰和B-spline曲线的无人机路径规划研究

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系统仿真学报 2024年第7期36卷 1509-1524页

作者：黄志锋刘媛华上海理工大学管理学院上海200082

针对无人机在动态环境中的全局路径规划问题,提出了一种改进哈里斯鹰优化算法。针对算法后期搜索性能不足等问题,提出自适应混沌和核心种群动态划分策略,提高算法后期的搜索能力;修改哈里斯鹰更新公式,引入黄金正弦策略,提高算法搜索效... 详细信息

针对无人机在动态环境中的全局路径规划问题,提出了一种改进哈里斯鹰优化算法。针对算法后期搜索性能不足等问题,提出自适应混沌和核心种群动态划分策略,提高算法后期的搜索能力;修改哈里斯鹰更新公式,引入黄金正弦策略,提高算法搜索效率;融合自适应动态云最优解扰动策略,提高算法跳出局部极值的能力;针对三维栅格路径规划问题,设置了一种估值函数,通过计算栅格到达终点的代价,帮助算法进行节点筛选,使算法能搜索到更短路径,并针对路径转角不平滑的问题,使用3次B-spline曲线对路径转角进行处理,使路径更适合无人机飞行。通过国际标准测试函数和在不同大小、不同复杂程度的静态、动态栅格地图进行仿真实验。实验结果显示,本文算法相较于对比算法,规划出的路径平均缩短了14.94%、转角数量平均减少了53.31%。

关键词：哈里斯鹰优化算法三维路径规划无人机动态环境自适应