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Design and Simulation of Single Hull Side Structure Based on Filling Sandwich

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Journal of Harbin Institute of Technology(New Series) 2019年第2期26卷 80-89页

作者： Langxiong Gan Hui Li Lei Zhang Chunhui Zhou yuanzhou Zheng Xiaobo Zhao School of Navigation Wuhan University of Technology Hubei Key Laboratory of Inland Shipping Technology The Development and Research Center of Yangtze River Shipping Nanjing Maritime Safety Administration of the People's Republic of China

Ship collision accidents are catastrophic accidents, and as the main energy-absorbing structure, the anti-collision capacity of the side structure has an important influence on the crashworthiness of the ship. It is necessary to improve the crashworthiness of the ship by increasing energy absorption of the side structure, in such ways as adding a filling sandwich in a single hull. The aim of the present study is to discuss the crashworthiness of the side structure stressed under lateral dynamic loads through simulation with the finite element code MSC/Dytran. The collision force, the damage deformation, the critical collision speed as well as the energy absorption of a side structure improved with a filling sandwich are compared to the standard structure through numerical simulations. The results indicate that a sandwich panel structure is an advanced ship protection structure that can remarkably improve the ability of the side structure to withstand collisions; the effect of the tube-style sandwich panel structure has the best results. The outcome of this study provides a reference for the crashworthiness assessment of side structures and the crashworthiness optimization design of new side structures.

关键词： side structure crashworthiness filling sandwich ship collision

Development of an ELISA kit using monoclonal antibody to Clostridium difficile toxin A

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World Journal of Gastroenterology 2004年第18期10卷 2747-2749页

作者： Si-WuFu Ya-LiZhang Dian-yuanzhou PLAInstituteforDigestiveMedicine NanfangHospitalFirstMilitaryMedicalUniversityGuangzhou510515GuangdongprovinceChina

AIM: To establish an ELISA kit using monoclonal antibodies against Clostridium diffidle ( C. difficile) toxin ***: An indirect sandwich ELISA was described using the purified rabbit monospecific antiserum as capturing antibody. After the polystyrene microtitre plates with 96 flat-bottomed wells were coated with rabbit antiserum,the wells were blocked with 100 g/L BSA in PBS-T. C. difficile toxin A or culture filtrates were added to each well and then monoclonal antibodies IgG-horseradish peroxidase conjugate was added as detecting antibody, tetramethylbenzidine was used as substrate and A450 of the stopped reacting product was recorded in an automated plate ***: The tested specimens included culture filtrates of 2 strains of toxigenic C. difficile, 2 strains of non-toxigenic C. difficile, 26 strains of E. coli 2 strains of S. dysenteriae,1 strain of Bif. infantis, 5 strains of V. cholera, 2 strains of S. typhi, 7 strains of C. botulinum, 1 strain of toxigenic C. sordllii, and 1 strain of C. butyricum. A total of 47 strains of culture filtrates were all negative except for 2 strains of toxigenic C. difficile. The detective limitation of toxin A was 0.1 ng/***: An ELISA kit with high specificity and excellent sensitivity for the rapid detection of C. difficile toxin A was established. It will be a useful tool for diagnostic test of C. difficile toxin A.

关键词： ELISA 单克隆抗体梭菌毒素A 血清

Liver transplantation for patients with hepatocellular carcinoma at the Liver Cancer Institute of Fudan University, China

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Chinese Medical Journal 2005年第8期118卷 654-659页

作者： ZHOUJian FANJia WUZhi-quan QIUShuang-jian HUANGXiao-wu YUYao WANGZheng SUNJian XIAOYong-sheng HEYi-feng YANGGuo-huan SONGKang yuanzhou WANGYu-qi TANGZhao-you LiverCancerInstitute ZhongshanHospitalFudanUniversityShanghai200032China

Background Selection of patients with hepatocellular carcinoma (HCC) for orthotopic liver transplantation (OLT) remains controversial. Since there is a trend to expand the transplant criteria for HCC patients, we reviewed the data of patients with HCC who had received OLT at our institute to determine their survival and prognostic *** A total of 67 patients with HCC who had undergone OLT from April 2001 through December 2003 were reviewed retrospectively. Selection OLT candidates with HCC was dependent on the anatomical characteristics and/or the severity of underlying liver cirrhosis. The 67 patients were followed up for more than 6 months after transplantation. Their survival rate was calculated by the Kaplan-Meier method. Univariate and multivariate analyses using the Cox proportional hazards regression model were performed to reveal the factors affecting the survival *** No perioperative death occurred in this series. The 1- and 2-year cumulative survival rates were 90.0% and 65.6%, and the disease-free survival (DFS) rates were 77.5% and 62.5% respectively. Univariate analysis revealed the tumor size, portal vein tumor thrombus (PVTT), serum alpha-fetoprotein level, bilobular distribution of tumors, pTNM stage and histological differentiation were statistically significant factors affecting the DFS ( P <0.05). Multivariate analysis showed tumor size and PVTT were independent and statistically significant factors affecting the DFS ( P =0.005 and 0.010, respectively). In this series, all but 2 received systemic chemotherapy, among them 13 had tumor recurrence within 8 months after OLT. Conclusions OLT is indicated for patients with HCC, even for some patients with end-stage liver disease who may survive longer without tumor recurrence. Adjuvant chemotherapy may decrease the recurrence of HCC after OLT.

关键词： liver transplantation · carcinoma hepatocellular· prognosis

Simplified purification method for Clostridium difficile toxin A

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World Journal of Gastroenterology 2004年第18期10卷 2756-2758页

作者： Si-WuFu JingXue Ya-LiZhang Dian-yuanzhou PLAInstituteforDigestiveMedicine NanfangHospitalFirstMilitaryMedicalUniversityGuangzhou510515GuangdongProvinceChina

AIM: To establish the purification method for Clostridium difficile ( C. difficile) toxin ***: C. difficile VPI 10463 filtrate was cultured anaerobically by the dialysis bag methods. And then the toxin A was purified by precipitation with 500 g/L (NH4)2SO4 and acid precipitation at pH 5.5, followed by ion-exchange chromatography on ***: Purified toxin A exhibited only one band on native polyacrylamide gel electrophoresis (native-PAGE) and Ouchterlony double immunodiffusion. The molecular weight of toxin A was estimated to be 550 000. The purified toxin A had a protein concentration of 0.881 mg/mL. The minimum lethal dose was 1×10^6 MLD/mL (***). The cytotoxic titer was 10^7 CU/mg. The haemagglutinate activity was at a concentration of 1.72 μg/mL. The ratio of fluid volume (mL) accumulated to the length (cm) of the loop was ***: The modified method for purification of toxin A of C difficile was simple and convenient. It may be even more suitable for purification of toxin A on large scales.

关键词：简单净化法梭菌毒素A 聚丙烯酰胺免疫扩散

Construction of attenuated Salmonella typhimurium Strain expressing Helicobacter pyloriconservative region of adhesin antigen and its immunogenicity

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World Journal of Gastroenterology 2004年第17期10卷 2498-2502页

作者： YangBai Ya-LiZhang Ji-DeWang Zhao-ShanZhang Dian-yuanzhou PLAInstituteforDigestiveMedicine NanfangHospitaltheFirstMilitaryMedicalUniversityGuangzhou510515GuangdongProvinceChina InstituteofBiotechnology AcademyofMilitaryMedicalSciencesBeijing100071China

AIM: To construct a non-resistant and attenuated Salmonella typhimurium ( S. typhimurium) strain which expresses conservative region of adhesion AB of Helicobacter pylori(Hpylon) and evaluate its ***: The AB gene amplified by PCR was inserted into the expression vector pYA248 containing asd gene and through two transformations introduced into the delta Cya1 delta Crp1 delta Asd attenuated Salmonella typhimurium strain, constructing balanced lethal attenuated Salmonella typhimurium strains X4072 (pYA248-AB). Bridged ELISA method was used to measure the expression of AB antigen in sonicate and culture supematant. According to the method described by Meacock, stability of the recombinant was evaluated. Semi-lethal capacity test was used to evaluate the safety of recombinant. The immunogenicity of recombinant was evaluated with animal ***: The attenuated S. typhimurium X4072 (pYA248-AB) which expresses AB was successfully ***, bridged ELISA assay showed that the content of AB in recombinant X4072 (pYA248- AB) culture supematant was higher than that was in thallus lytic liquor. And after recombinant X4072 (pYA248- AB) was cultured for 100 generations without selection pressure, bhe entire recombinant bacteria selected randomly could grow, and the AB antigen was defected positive by ELISA. The growth curve of the recombinant bacteria showed that the growth states of X4072 (pYA248) and X4072 (pYA248-AB) were basically consistent. The survival rate of C57BL/6 was still 100%, at 30 d after mice taking X4072 (pYA248-AB) 1.0×l0^10 cfu orally. Oral immunization of mice with X4072 (pYA248-AB) induced a specific immune ***: In vitro recombinant plasmid appears to be stable and experiments on animals showed that the recombinant strains were safe and immunogenic in vitro,which providing a new live oral vaccine candidate for protection and care of H pylori infection.

关键词：沙门氏菌属盲肠疾病疲劳反应哈比特属幽门菌附着因子保守治疗抗原免疫原性消化系统

Research on Establish an Efficient Log Analysis System with Kafka and Elastic Search

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Journal of Software Engineering and Applications 2017年第11期10卷 843-853页

作者： yuanzhou Wei Manman Li Binsen Xu Beihang University Beijing China Henan University of Economics and Law Zhengzhou China

Search logs in a timely and efficient manner are an important part of SRE (Site Reliability Engineer). Logs help us solve the problems during our development work. In this paper, we will introduce you a way how to build an efficient logs analysis system based on kafka and Elastic Search. We hope you can learn something through the iteration of the Version and get some inspiration with your own log analysis system.

关键词： Log Analysis System Kafka Elastic Search Software Applications Site Reliability Engineer

Pathogenicty and immune prophylaxis of cag pathogenicity island gene knockout homogenic mutants

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World Journal of Gastroenterology 2004年第22期10卷 3289-3291页

作者： Huan-JianLin JingXue YangBai Ji-DeWang Ya-LiZhang Dian-yuanzhou PLAInstituteofDigestiveMedicine NanFangHospitaltheFirstMilitaryMedicalUniversityGuangzhou510515GuangdongProvinceChina

AIM: To clarify the role of cag pathogenicity island (cagPAI) of Helicobacter pylori (H pylori) in the pathogenicity and immune prophylaxis of H pylori infection. METHODS: Three pairs of H pylori including 3 strains of cagPAI positive wildtype bacteria and their cagPAI knockout homogenic mutants were utilized. H pylori binding to the gastric epithelial cells was analyzed by flow cytometry assays. Apoptosis of gastric epithelial cells induced by H pylori was determined by ELISA assay. Prophylaxis effect of the wildtype and mutant strains was compared by immunization with the sonicate of the bacteria into mice model. RESULTS: No difference was found in the apoptasis between cagPAI positive and knockout H pylori strains in respective of the ability in the binding to gastric epithelial cells as well as the induction of apoptosis. Both types of the bacteria were able to protect the mice from the infection of H pylori after immunization, with no difference between them regarding to the protection rate as well as the stimulation of the proliferation of splenocytes of the mice. CONCLUSION: The role of cagPAI in the pathogenicity and prophylaxis of Hpyloriinfection remains to be cleared.

关键词：致病性免疫学预防作用病原性脑岛基因同基因突变异种 H幽门菌

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抗日战争时期陕甘宁边区的新文字冬学运动

抗日战争研究 2009年第3期 43-51页

作者：王元周北京大学历史学系

近代以来,在西势东渐的压力下,中国人渐对民族文化失去信心,转而追求彻底的变革。文字改革,即以拼音文字取代汉字的主张也是这种反传统倾向的一种表现。随着时代的变化,这种主张越来越政治化,最终一部分共产党人成为这种主张的坚决拥护... 详细信息

近代以来,在西势东渐的压力下,中国人渐对民族文化失去信心,转而追求彻底的变革。文字改革,即以拼音文字取代汉字的主张也是这种反传统倾向的一种表现。随着时代的变化,这种主张越来越政治化,最终一部分共产党人成为这种主张的坚决拥护者和积极实践者,从土地革命时期开始进行新文字教育实践,在抗日战争时期更试图通过冬学等群众性识字运动推行新文字。陕甘宁边区于1940年开始在冬学运动中进行新文字教育试点,1941年冬学运动期间普及到全边区,然而很快于1942年冬学运动期间再次将新文字冬学实施范围缩小到延安县,随后停止了新文字推行工作。这次推行新文字工作的经验教训在当时并没有得到很好的总结,从而影响到中华人民共和国成立后的文字改革。

关键词：抗日战争陕甘宁边区文字改革新文字冬学运动

Cloning and expression and immunogenicity of Helicobacter pylori BabA_2 gene

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World Journal of Gastroenterology 2004年第17期10卷 2560-2562页

作者： YangBai Ya-LiZhang YeChen Jian-FengJin Zhao-ShanZhang Dian-yuanzhou PLAInstituteforDigestiveMedicine NanfangHospitaltheFirstMilitaryMedicalUniversityGuangzhou510515GuangdongProvinceChina ChemistryuniversityofBeijing Beijing100071China InstituteofBiotechnology AcademyofMilitaryMedicalSciencesBeijing100071China

AIM: To construct a recombinant strain which expresses BabA of Helicobacter pylori(H pylori) and to study the immunogenicity of ***: BabA2 DNA was amplified by PCR and inserted into the prokaryotie expression vector pET-22b (+) and expressed in the BL21 (DE3) *** strain. Furthermore,BabA immunogenicity was studied by animal ***: DNA sequence analysis showed the sequence of BabA2 DNA was the same as the one published by *** BabA recombinant protein accounted for 34.8% of the total bacterial protein. The serum from H pylori infected patients and Balb/c miced immunized with BabA itself could recognize ***: BabA recombinant protein may be an potential vaccine for control and treatment of Hpyloriinfection.

关键词：无性繁殖基因表达免疫原性哈比特属幽门菌 BabA2基因重组细胞蛋白