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Effects of HMGB1 Expression Suppressed by siRNA on Cell Cycle and Proliferation of Human Cervical Cancer Cell Line HeLa

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Clinical oncology and cancer researeh 2010年第2期7卷 128-133页

作者： yuan-yuan qiu hui-yu wang quan hao Key Laboratory of Cancer Prevention and Therapy Tianjin Department of Gynecology Cancer Institute and Hospital of Tianjin Medical University Tianjin 300060 China.

OBJECTIVE In this study, RNA interference was used to evaluate the effects of HMGB1 expression on cell cycle and proliferation of the human cervical cancer cell line *** We had previously constructed and screened effective eukaryotic expression vectors carrying PGCsi3.0-1/ HMGB1 siRNA and PGCsi3.0-3/HMGB1 siRNA, then the vectors were transfected into HeLa cells. The expression of HMGB1 before and after transfection in HeLa cells were detected by RT-PCR and Western blot. The cell viability and proliferating activity was tested by Trypan blue dye test and MTT, and the cell cycle was determined bv flow *** The introduction of PGCsi3.0-1/HMGB1 siRNA and PGCsi3.0-3/HMGB1 siRNA inhibited the expression of HMGB1 mRNA and protein efficiently and specifically, there was a significant difference between the siRNA groups and the control groups （P 〈 0.05）. The proliferation speed of PGCsi3.0-1 group and PGC si3.0-3 group were obviously slower than those of PGCsi3.0- Neg group and non-transfected group. Flow cytometry showed that the content of DNA in G2 phase in PGCsi3.0-1 group and PGCsi3.0-3 group were obviously more than those in PGCsi3.0- Neg group and non-transfected group, but the content in S phase was less （P 〈 0.01）. The progression of cell cycle was arrested from G2 to S *** PGCsi3.0-1/HMGB1 siRNA and PGCsi3.0-3 /HMGB1 siRNA could specially suppress the expression of HMGB1 gene, inhibit the proliferation speed of HeLa cells effectively, and arrest the progression of cell cycle from G2 to S phase. RNAi provides a new approach to the bio-therapy of cervical cancer.

关键词： high mobility group box-l HeLa cells RNAinterference cell proliferation cell cycle.

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冲击质量对方形锂离子电池在冲击载荷下动态力学响应和失效模式的影响

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爆炸与冲击 2025年第2期45卷 47-62页

作者：王涛孟康培刘跃专袁泉黄浩陈晓平宁波工程学院机械与汽车工程学院浙江宁波315016 长安大学能源与电气工程学院陕西西安710064

电动汽车在运行过程中容易发生碰撞事故,动力锂离子电池不可避免会受到冲击作用,导致电池不同程度的损伤,而损伤程度的判断对电池的安全使用至关重要。基于上述背景,开展了不同冲击质量对方形锂离子电池动态冲击响应和失效行为影响的研... 详细信息

电动汽车在运行过程中容易发生碰撞事故,动力锂离子电池不可避免会受到冲击作用,导致电池不同程度的损伤,而损伤程度的判断对电池的安全使用至关重要。基于上述背景,开展了不同冲击质量对方形锂离子电池动态冲击响应和失效行为影响的研究。首先,开展了准静态压缩试验,采用6种不同的进给速度对锂离子电池进行了挤压测试。试验结果显示,随着进给速度的递增,锂离子电池达到硬短路失效所需的峰值载荷持续减小。这表明,准静态条件下,锂离子电池的短路失效载荷主要由挤压速度决定。然后,开展了落锤冲击试验,通过调控冲头的质量和冲击速度,系统模拟了锂离子电池可能遭遇的多种冲击工况。研究结果表明,冲击质量和冲击速度是决定锂离子电池动态失效行为的关键因素。在冲击能量恒定的条件下,相较于高速、小质量的冲击条件,低速、大质量的冲头冲击对电芯内部造成的损伤更明显;当冲头质量不变时,提高冲击速度则会提前触发锂离子电池的内短路。

关键词：方形锂离子电池冲击质量冲击速度冲击能量动态失效

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Aerobic granular sludge formation based on substrate availability:Effects of flow pattern and fermentation pretreatment

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Frontiers of Environmental Science & Engineering 2020年第3期14卷 155-164页

作者： quan yuan hui Gong hao Xi Kaijun wang State Key Joint Laboratory of Environment Simulation and Pollution Control School of EnvironmentTsinghua UniversityBeijing 100084China

The influences of flow patterns(mixed-flow and plug-flow)and fermentation pretreatment on aerobic granular sludge(AGS)formation with various substrate availability levels were investigated by running four identical laboratory-scale sequencing batch reactors(R1-R4),comparing two anaerobic feeding strategies and three kinds of substrates.R1 achieved faster granulation with a fast influent fill step followed by a modified anaerobic mixed-flow phase,but the AGS showed poorer stability with a cracked structure and a high suspended solids concentration in the *** anaerobic plug-flow feeding mode(with influent fed slowly from the bottom)in R2 provided deeper penetration depth for the substance to reach the core of A G S and accordingly strengthen A G S *** acidogenic upflow sludge bed reactor was introduced as a pretreatment to improve the AGS performance by enhancing glucose pre-fermentation(R4).AGS fed with mixed volatile fatty acids(VFA)after glucose fermentation showed similar performance compared with the reactor fed with acetate in the aspects of stability,structure,size distribution and nitrogen removal efficiency,and 74%similarity in the microbial *** actual wastewater with low VFA concentrations,fermentation treatment was suggested as a promising pretreatment for stable AGS granulation and operation.

关键词： Anaerobic plug-flow feeding mode Anaerobic mixed-flow mode Fermentation pretreatment Substrate gradient Penetration depth

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速率自适应的海面物联网跨层机会传输协议

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控制与决策 2025年第1期40卷 180-186页

作者：赵瑞琴张东健全豪刘创源王海燕西北工业大学航海学院西安710072 海洋声学信息感知工业和信息化部重点实验室(西北工业大学) 西安710072 西北工业大学国家卓越工程师学院西安710072 陕西科技大学电子信息与人工智能学院西安710071

海面物联网在海洋观测、海域监测等应用中发挥着重要作用.复杂动态海洋环境造成海洋网络链路状态、链路质量的时变特征,且其给海面物联网带来了严峻挑战,为此,将物理层自适应数据速率、媒体访问控制(MAC)层载波侦听多址接入与网络层机... 详细信息

海面物联网在海洋观测、海域监测等应用中发挥着重要作用.复杂动态海洋环境造成海洋网络链路状态、链路质量的时变特征,且其给海面物联网带来了严峻挑战,为此,将物理层自适应数据速率、媒体访问控制(MAC)层载波侦听多址接入与网络层机会路由联合进行跨层设计,提出速率自适应海面物联网跨层传输协议,通过3次握手机制机会性选择最佳转发结点并基于动态信噪比实现传输速率实时自适应调整,旨在提高动态复杂海洋环境下海面物联网的稳健性.陆地和湖上试验结果表明,所提协议在数据包交付率、能耗与时延方面性能优异,可为复杂动态海洋环境中海面物联网数据的高效传输提供重要的技术途径.

关键词：海洋物联网机会路由网络速率自适应 LoRa 海洋链路

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An aqueous rechargeable zinc-ion battery on basis of an organic pigment

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Rare Metals 2022年第7期41卷 2230-2236页

作者： Feng yu Yi wang yu Liu hao-yuan hui Fa-Xing wang Jing-Fa Li quan wang School of Chemistry and Materials Science Nanjing University of Information Science and TechnologyNanjing 210044China School of Chemical and Environmental Engineering Yancheng Teachers UniversityYancheng 224000China Department of Chemistry and Food Chemistry and Center for Advancing Electronics Dresden(Cfaed) Technische Universita¨t DresdenDresden 01062Germany School of Materials Engineering Changshu Institute of TechnologyChangshu 215500China

Neutral aqueous rechargeable zinc-ion batteries are receiving continuous attention because of their advantages of low cost,high safety,environmental friendliness,and high performance,which are difficult to attain with... 详细信息

关键词： battery electrolyte recharge

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Strategies to improve aerobic granular sludge stability and nitrogen removal based on feeding mode and substrate

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Journal of Environmental Sciences 2019年第10期31卷 144-154页

作者： quan yuan hui Gong hao Xi Heng Xu Zhengyu Jin Nasir Ali Kaijun wang State Key Joint Laboratory of Environment Simulation and Pollution Control School of EnuironmentTsinghua Uniuersity

A systemic strategy was proposed to improve aerobic granular sludge(AGS)stability and nitrogen(N)removal efficiency by optimizing feeding mode and substrate aiming at complicated wastewater *** functional groups at the genus level identified by high-throughput sequencing were evaluated as *** results showed that anaerobic feeding mode and acetate promoted the compact AGS formation with excellent total nitrogen(TN)removal efficiency(averaging 91.7%±4.1%)at various dissolved oxygen *** the aerobic feeding mode led to a loose AGS structure with a vulnerable anaerobic core and poor TN removal efficiency(averaging58.8%±7.4%).Simultaneous nitrification and denitrification process played the dominant role in N removal in compact AGS over the alternating nitrification and denitrification ***-concentration glucose undermined feast-famine condition with filamentous bacteria growth out of granule and decreased TN removal efficiency to 67.3%±15.2%.Lower food to microorganism ratio may result in a lower N removal rate attributed to the sharply increased biomass concentration fed by ***-oxidizing bacteria,nitrite-oxidizing bacteria,denitrifying bacteria,and denitrifying phosphorus accumulation organisms enriched during AGS granulation also contributed to the efficient N *** proposed strategy provided insights into the relationship between various factors and stable AGS formation,and systemic operation methods for various complicated wastewater treatment.

关键词： Aerobic granular sludge Feeding mode Substrate N removal Stability

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Numerical studies on issues of Re-independence for indoor airflow and pollutant dispersion within an isolated building

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Building Simulation 2022年第7期15卷 1259-1276页

作者： Peng-Yi Cui Wei-qiu Chen Jia-Qi wang Jin-hao Zhang yuan-Dong Huang Wen-quan Tao College of Environment and Architecture University of Shanghai for Science and TechnologyShanghaiChina Key Laboratory of Thermo-Fluid Science and Engineering of MOE School of Energy and Power EngineeringXi'an Jiaotong UniversityXi’anChina

This study conducted the numerical models validated by wind-tunnel experiments to investigate the issues of Re-independence of indoor airflow and pollutant dispersion within an isolated *** window Reynolds number(Re_(w))was specified to characterize the indoor flow and *** indicators of RRC(ratio of relative change)or DR(K_DR)(difference ratio of dimensionless concentration)quantitatively determine the critical Rew for indoor flow and turbulent *** results show that the critical Re(Re_(crit)) value is position-dependent,and Re_(crit) at the most unfavorable position should be suggested as the optimal value within the whole areas of *** Re(H,orit)=27,000 is recommended for the outdoor flows;while Re_(w,crit)=15,000 is determined for the indoor flows due to the lower part below the window showing the most *** suggested Re_(w,crit)(=15,000)for indoor airflow and cross ventilation is independence of the window ***,taking K_DR≤5% as the indicator,the suggested Re_(w,crit) for ensuring indoor pollutant diffusion enter the independence regime should also be 15,000,indicating that indoor passive diffusion is completely determined by the flow *** contours of dimensionless velocity(U/U_(0))and concentration(K)against the increasing Re(w) further confirmed this critical *** study further reveals the Re-independence issues for indoor flow and dispersion to ensure the reliability of the data obtained by reduced-scale numerical or wind-tunnel models.

关键词： CFD simulation Re-independence similarity criterion indoor ventilation pollutant dispersion

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非洲猪瘟病毒I7L蛋白参与病毒免疫逃逸的新机制

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中国兽医科学 2025年第1期55卷 139-140页

作者：陈佳宁中国农业科学院兰州兽医研究所

非洲猪瘟（ASF）是猪的一种急性，热性，高度接触性传染病，致死率可达100%。非洲猪瘟病毒（ASFV）是引起该病的病原。ASFV主要感染原代猪单核巨噬细胞系统，仅低效感染少数细胞系，这极大地制约了相应疫苗的研发及对ASFV病原生物学及... 详细信息

非洲猪瘟（ASF）是猪的一种急性，热性，高度接触性传染病，致死率可达100%。非洲猪瘟病毒（ASFV）是引起该病的病原。ASFV主要感染原代猪单核巨噬细胞系统，仅低效感染少数细胞系，这极大地制约了相应疫苗的研发及对ASFV病原生物学及致病机制的探索。近期，PLoS Pathogens杂志在线发表了题为“The I7L protein of African swine fever virus is involved in viral pathogenicity by antagonizing the IFN-γ-triggered JAK-STAT signaling pathway through inhibiting the phosphorylation of STAT1”的研究论文，中国农业科学院哈尔滨兽医研究所孙元研究员、李素研究员、仇华吉研究员和李连峰研究员为该论文的共同通讯作者。研究证实，ASFV基因组编码的I7L蛋白可结合STAT1，从而抑制STAT1的磷酸化并拮抗IFN-γ介导的JAK-STAT信号通路，进而调控病毒致病力。该研究为开发ASF弱毒活疫苗提供了新靶点。

关键词：非洲猪瘟病毒(ASFV) I7L蛋白免疫逃逸

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The effect of salt anion in ether‐based electrolyte for electrochemical performance of sodium‐ion batteries:A case study of hard carbon

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Carbon Energy 2024年第8期6卷 285-297页

作者： Jiabao Li Jingjing hao quan yuan Ruoxing wang Frederick Marlton Tianyi wang Chengyin wang Xin Guo Guoxiu wang College of Chemistry and Chemical Engineering Yangzhou UniversityYangzhouJiangsuChina School of Mathematical and Physical Sciences Centre for Clean Energy TechnologyFaculty of ScienceUniversity of Technology SydneySydneyAustralia

Compared with the extensively used ester‐based electrolyte,the hard carbon(HC)electrode is more compatible with the ether‐based counterpart in sodium‐ion batteries,which can lead to improved cycling stability and robust rate ***,the impact of salt anion on the electrochemical performance of HC electrodes has yet to be fully *** this study,the anionic chemistry in regulating the stability of electrolytes and the performance of sodium‐ion batteries have been systematically *** work shows discrepancies in the reductive stability of the anionic group,redox kinetics,and component/structure of solid electrolyte interface(SEI)with different salts(NaBF_(4),NaPF_(6),and NaSO_(3)CF_(3))in the typical ether solvent(diglyme).Particularly,the density functional theory calculation manifests the preferred decomposition of PF_(6)−due to the reduced reductive stability of anions in the solvation structure,thus leading to the formation of NaF‐rich *** investigation on redox kinetics reveals that the NaPF_(6)/diglyme can induce the fast ionic diffusion dynamic and low charge transfer barrier for HC electrode,thus resulting in superior sodium storage performance in terms of rate capability and cycling life,which outperforms those of NaBF_(4)/diglyme and NaSO_(3)CF_(3)/***,this work offers valuable insights for optimizing the electrochemical behaviors of electrode materials by regulating the anionic group in the electrolyte.

关键词： ether‐based electrolyte reaction kinetics salt anion SEI components sodium storage

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Discharge image reconstruction and frequency domain analysis based on event data

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High Voltage 2024年第6期9卷 1195-1201页

作者： quan yuan Lei Deng hao Guo Qishen Lyu Xin Zhang Jibin Wu yu Deng Hongyang Zhou Xilin wang Zhidong Jia Engineering Laboratory of Power Equipment Reliability in Complicated Coastal Environments Tsinghua Shenzhen International Graduate SchoolTsinghua UniversityShenzhenGuangdongChina Department of Precision Instrument Center for Brain Inspired Computing ResearchTsinghua UniversityBeijingChina College of Information and Computer Taiyuan University of TechnologyTaiyuanChina Shenzhen Power Supply Corporation China Southern Power Grid CorporationShenzhenChina Department of Computing The Hong Kong Polytechnic UniversityHong KongChina China Electric Power Research Institute BeijingChina School of Electrical Engineering and Automation Xiamen University of TechnologyXiamenChina

Optical image method has been the earliest and most used direct method for observing gas ***,research on gas discharge monitoring based on visible light mainly relies on high-speed cameras,but the large size,significant data storage requirements,and susceptibility to interference from complex backgrounds and lighting conditions limit their further *** vision sensing(DVS)technology is a neuromorphic sensing technology that asynchronously measures the luminance changes at each *** offers advantages such as a large dynamic range(>120 dB),high temporal resolution(up to 1µs),and small data volume(MB level).In this study,dynamic vision sensing technology was employed to monitor both 30 mm short-gaps and 1080 mm long-gaps discharge processes *** study developed the CountImage encoding method for event data and conducted image reconstruction,time-domain analysis,and frequency-domain charac-teristic analysis based on the event *** results show that the event-reconstructed images are highly consistent with the high-speed camera images,and the arc develop-ment process and its path can also be clearly ***,this study discovered a correlation between the electrical characteristics and event information during the discharge *** the time domain,the duration of the maximum DVS event count closely matches the duration during which the voltage drops to zero during *** the frequency domain,the Pearson correlation coefficient between the event stream spectrum and the voltage signal spectrum is greater than *** the maximum number of brightening events(ONmax)and the maximum number of darkening events(OFFmax)are positively correlated with the voltage applied between the *** study demonstrates that,compared to the GB/s data rate of high-speed cameras,this approach can record the discharge process and accurately reconstruct the discharge process,arc morphology,and discharge path at M

关键词： image analysis holds

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