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MiR-204-3p overexpression inhibits gastric carcinoma cell proliferation by inhibiting the MAPK pathway and RIP1/MLK1 necroptosis pathway to promote apoptosis

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World Journal of Gastroenterology 2023年第29期29卷 4542-4556页

作者： Xia Li Joanna J Tibenda Yi Nan Shi-Cong Huang Na Ning Guo-qing Chen Yu-Hua Du Ya-Ting Yang Fan-Di Meng Ling yuan College of Pharmacy Ningxia Medical UniversityYinchuan 750004Ningxia Hui Autonomous RegionChina Ningxia Chinese Medicine Reserch Center Yinchuan 750004Ningxia Hui Autonomous RegionChina Key Laboratory of Hui Ethnic Medicine Modernization of Ministry of Education Ningxia Medical UniversityYinchuan 750004Ningxia Hui Autonomous RegionChina Traditional Chinese Medicine College Ningxia Medical UniversityYinchuan 750004Ningxia Hui Autonomous RegionChina

BACKGROUND Gastric carcinoma(GC)is the third most frequent cause of cancer-related death,highlighting the pressing need for novel clinical treatment *** this regard,microRNAs(miRNAs)have emerged as a promising therapeutic *** have shown that miRNAs can regulate related signaling pathways,acting as tumor suppressors or tumor *** To explore the effect of miR-204-3p on GC *** We measured the expression levels of miR-204-3p in GC cells using quantitative real-time polymerase chain reaction,followed by the delivery of miR-204-3p overexpression and miR-204-3p knockdown vectors into GC ***-8 was used to detect the effect of miR-204-3p on the proliferation of GC cells,and the colony formation ability of GC cells was detected by the clonal formation *** effects of miR-204-3p on GC cell cycle and apoptosis were detected by flow *** BABL/c nude mouse subcutaneous tumor model using MKN-45 cells was constructed to verify the effect of miR-204-3p on the tumorigenicity of GC ***,the study investigated the effects of miR-204-3p on various proteins related to the MAPK signaling pathway,necroptosis signaling pathway and apoptosis signaling pathway on GC cells using Western blot *** Firstly,we found that the expression of miR-204-3p in GC was *** treated with the lentivirus overexpression vector,miR-204-3p expression significantly increased,but the lentivirus knockout vector had no significant effect on *** vitro experiments confirmed that miR-204-3p overexpression inhibited GC cell viability,promoted cell apoptosis,blocked the cell cycle,and inhibited colony formation *** vivo animal experiments confirmed that miR-204-3p overexpression inhibited subcutaneous tumorigenesis ability in BABL/c nude ***,our results verified that miR-204-3p overexpression can inhibit GC cell proliferation by inhibiting protein expression levels of KRAS and p-ERK1/2 in the MAPK pa

关键词： miR-204-3p Gastric carcinoma MAPK signaling pathway Apoptosis Necroptosis

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On the development of modular polyurethane-based bioelastomers for rapid hemostasis and wound healing

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Regenerative Biomaterials 2023年第1期10卷 640-654页

作者： Wanxin Guo Binan Zhao Muhammad Shafiq Xiao Yu Yihong shen Jie Cui Yujie Chen Pengfei Cai Zhengchao yuan Mohamed EL-Newehy Hany EL-Hamshary Yosry Morsi Binbin Sun Jianfeng Pan Xiumei Mo State Key Laboratory for Modification of Chemical Fibers and Polymer Materials Shanghai Engineering Research Center of Nano-Biomaterials and Regenerative MedicineCollege of Biological Science and Medical EngineeringDonghua UniversitySongjiangShanghai 201620P.R.China Department of Orthopedics Shanghai Tongji HospitalSchool of MedicineTongji UniversityPutuoShanghai 200065China Department of Chemical Engineering Faculty of EngineeringGraduate SchoolKyushu UniversityFukuoka 819-0395Japan Department of Chemistry College of ScienceKing Saud UniversityRiyadh 11451Saudi Arabia Faculty of Engineering and Industrial Sciences Swinburne University of TechnologyBoroondaraVIC 3122Australia Department of Orthopedics Shanghai Tenth People’s HospitalSchool of MedicineTongji UniversityJinganShanghai 200072China

Massive hemorrhage may be detrimental to the patients,which necessitates the advent of new materials with high hemostatic efficiency and good *** objective of this research was to screen for the effect of the different types of bio-elastomers as hemostatic dressings.3D loose nanofiber sponges were prepared;PU-TA/Gel showed promising ***(PU)was synthesized and electrospun to afford porous sponges,which were crosslinked with glutaraldehyde(GA).FTIR and 1H-NMR evidenced the successful synthesis of *** prepared PU-TA/Gel sponge had the highest porosity and water absorption ***,PU-TA/Gel sponges exhibited cytocompatibility,negligible hemolysis and the shortest clotting ***-TA/Gel sponge rapidly induced stable blood clots with shorter hemostasis time and less bleeding volume in a liver injury model in ***,PU-TA/Gel sponges also induced good skin regeneration in a full-thickness excisional defect model as revealed by the histological *** results showed that the PU-TA/Gel-based sponges may offer an alternative platform for hemostasis and wound healing.

关键词： polyurethane electrospinning hemostasis material wound healing tissue regeneration regenerative medicine

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'新郁'葡萄microRNA的鉴定及其高温胁迫响应分析

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农业生物技术学报 2024年第9期32卷 2009-2020页

作者：牛丽丽武云龙刘丽媛宋洋波曹红燕杜婷婷王胜杰郗琳杨清孟冬周慧何坤祖中国医学科学院北京协和医学院药用植物研究所吐鲁番市葡萄产业发展促进中心青海大学农牧学院北京林业大学林学院霍恩海姆大学吐鲁番丝路明珠农业生物科技有限公司

高温胁迫是制约果树生产的主要逆境胁迫之一。葡萄(Vitis vinifera)是世界范围内具有较高经济价值的栽培水果作物，其生长发育经常受到高温胁迫的影响。研究葡萄高温胁迫响应机制对了解葡萄对高温胁迫的驯化具有重要意义。本研究采集'新... 详细信息

高温胁迫是制约果树生产的主要逆境胁迫之一。葡萄(Vitis vinifera)是世界范围内具有较高经济价值的栽培水果作物，其生长发育经常受到高温胁迫的影响。研究葡萄高温胁迫响应机制对了解葡萄对高温胁迫的驯化具有重要意义。本研究采集'新郁'葡萄叶片进行高通量测序，分别构建了对照组和高温胁迫处理组的小RNA (small RNA, sRNA)文库，鉴定了一系列高温应激反应miRNA，并分析了其在高温耐受反应中的功能。共鉴定得到171个已知miRNA和251个新miRNA，其中6个已知miRNA和21个新鉴定miRNA在高温胁迫下差异表达，预测获得这些差异表达的miRNA靶基因共计297个。通过qRTPCR对筛选出的10个miRNA及其靶基因表达进行了验证，结果表明这些靶基因对高温胁迫有明显响应。基因功能和通路分析表明，这些基因可能在耐高温胁迫中起重要作用。本研究结果为进一步了解果树驯化过程中的高温胁迫反应以及选育耐高温胁迫葡萄品种提供了理论基础。

关键词：葡萄 miRNA 高温胁迫高通量测序

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Combined Antitumor Effect of Ursolic Acid And 5-Fluorouracil on Human Esophageal Carcinoma Cell Eca-109 In Vitro

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Chinese Journal of Cancer Research 2010年第1期22卷 62-67页

作者： Guo-qing Chen Zhen-wei Yao Wei-ping Zheng Li Chen Hong Duan Yi shen Research Center of Tissue Engineering and Stem cell Department of Pathophysiology Chongqing Medical University Chongqing 400016 China Department of Obsttrics and Gynecology the First Affiliated Hospital Chongqing Medical University Chongqing 400016 China

Objective： To study the combined antitumor effect and possible mechanisms of ursolic acid with 5-fluorouracil （5-FU） on human esophageal carcinoma cell Eca-109 in vitro. Methods： Eca-109 cells were treated with ursolic acid （10-50 μmol/L） and/or 5-fluorouracil （48.0-768.8 μmol/L） for 48 h in vitro. And then cell proliferation was determined by MTT assay. Cell cycle and apoptosis rate were analyzed by flow cytometry （FCM）. The morphological changes of apoptosis were observed by fluorescent microscopy. At last the expression of P27kipl, bcl-2 and bax were detected by western blot. Results： Results： In comparison with single agent treatment, the combination of ursolic acid and 5-fluorouracil produced greater efficacy in growth inhibition, cell cycle arrest at G0/G1 phase, and apoptosis induction （P〈0.05）. Western blot analysis showed that the combination use of ursolic acid and 5-fluorouracil suppressed the expression of bcl-2 and increased the expressions of bax and P27kip1. Conclusion： Ursolic acid combined with 5-fluorouracil showed adjuvant antiproliferative effects on human esophageal carcinoma cell Eca-109 in vitro, which mainly due to the induction of cell cycle arrest as well as apoptosis.

关键词： Ursolic acid 5-fluorouracil Eca-109 cells Apoptosis Cell cycle P27kip1

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骶四针穴针刺联合生物反馈治疗压力性尿失禁疗效观察

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实用中医药杂志 2024年第9期40卷 1848-1850页

作者：袁庆郑芳赵恒飞卢先彬高龙田春燕张献辉吴毅重庆市垫江县人民医院重庆垫江408300

目的:观察骶四针穴针刺联合生物反馈治疗压力性尿失禁(SUI)的效果。方法:60例随机分为对照组和研究组各30例。两组均给予生物反馈治疗,研究组加用骶四针穴针刺。结果:治疗后两组盆底表面肌电值均升高,且研究组高于对照组(P<0.05)。两组1... 详细信息

目的:观察骶四针穴针刺联合生物反馈治疗压力性尿失禁(SUI)的效果。方法:60例随机分为对照组和研究组各30例。两组均给予生物反馈治疗,研究组加用骶四针穴针刺。结果:治疗后两组盆底表面肌电值均升高,且研究组高于对照组(P<0.05)。两组1h尿垫试验尿失禁量均礒少,且研究组少于对照组(P<0.05)。两组ICI-Q-SF量表评分均降低,且研究组低于对照组(P<0.05)。总有效率研究组高于对照组(P<0.05)。结论:骶四针穴针刺联合生物反馈治疗SUI疗效较好。

关键词：压力性尿失禁骶四针穴针刺生物反馈对照治疗观察

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Inhibition of pancreatic carcinoma cell growth in vitro by DPC4 gene transfection

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World Journal of Gastroenterology 2008年第40期14卷 6254-6260页

作者： Wei shen Guo-qing Tao Bing Cai De-Chun Li Xing-Guo Zhu Xia Bai Department ofGeneral Surgery Wuxi People's Hospital Wuxi 214023Jiangsu Province China General surgery department of thefirst affiliated hospital to Suzhou University Suzhou 215007Jiangsu Province China Jiangsu Institute of Hematology first affiliated hospitalto Suzhou University Suzhou 215007 Jiangsu Province China

AIM: To detect the expression of DPC4 in malignant and non-malignant specimens of human pancreas,and observe the inhibition of retroviral pLXSN containing DPC4 on pancreatic carcinoma cells in ***: The expression of DPC4 was determined in 40 pancreatic adenocarcinoma and 36 non-malignant pancreatic specimens by reverse-transcriptase polymerase chain reaction (RT-PCR) and ***,we constructed retroviral vectors containing DPC4,which then infected the pancreatic carcinoma cell line *** growth in vitro after being infected was observed,and the vascular endothelial growth factor (VEGF) mRNA level in the daughter cells was determined by semi-quantitative PCR ***: The RT-PCR assay showed a positive rate of DPC4 mRNA in 100% (36/36) of normal specimens,compared to 40% (16/40) in adenocarcinoma *** regional and intense positive cases of DPC4 expression in adenocarcinoma detected by immunohistochemistry were 10 and four,whereas it was all positive expression in normal *** was a significant difference of DPC4 expression between *** stable expression of DPC4 in the pancreatic carcinoma cells BxPC-3 could be resumed by retroviral vector pLXSN transfection,and could inhibit cell growth in ***,DPC4 could decrease VEGF mRNA transcription ***: The deletion of DPC4 expression in pancreatic carcinoma suggests that loss of DPC4 may be involved in the development of pancreatic *** retroviral vector pLXSN containing DPC4 can inhibit the proliferation of pancreatic carcinoma cells,and down-regulate the level of VEGF.

关键词： Gene therapy Pancreatic carcinoma Retroviral vector

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塔式喷淋与活性焦组合工艺在污泥好氧发酵废气领域的应用研究

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四川环境 2024年第2期43卷 180-184页

作者：彭辉辉张倩倩高爱华黄克毅常兴涛王庆元郑州市污水净化有限公司郑州450000

污泥好氧发酵产生的废气具有成分复杂、臭气浓度高等特点,难以被传统的生物除臭法所去除,除臭问题成为制约污泥好氧发酵的瓶颈问题。郑州某污泥处理厂采用化学吸收、火山岩除湿和活性焦吸附等组合新技术解决这一难题。结果表明:化学吸... 详细信息

污泥好氧发酵产生的废气具有成分复杂、臭气浓度高等特点,难以被传统的生物除臭法所去除,除臭问题成为制约污泥好氧发酵的瓶颈问题。郑州某污泥处理厂采用化学吸收、火山岩除湿和活性焦吸附等组合新技术解决这一难题。结果表明:化学吸收法可以将空间和堆体氨气浓度降低至15mg/m^(3)和20mg/m^(3)以下,去除率高达85%,喷淋方式可以将堆体气体温度从62℃降低至40℃,臭气浓度在此阶段也得到了大幅度减少。经过火山岩除湿系统,可以将气体相对湿度从80%降低至50%左右,可延长活性焦的使用寿命。而活性焦吸附则主要去除好氧发酵气体废气中的非极性气体,将臭气浓度控制在1000以下,使气体稳定达标排放。

关键词：污泥好氧发酵挥发性气体活性焦非极性气体

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黄连配方颗粒与标准汤剂化学成分一致性研究

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中成药 2024年第9期46卷 2851-2858页

作者：张代亮董新娜史磊董晓弟林永强张荣飞张京华姚元成张风超曹桂云孟兆青山东中医药大学山东济南250355 山东宏济堂制药集团股份有限公司山东济南250103 山东省食品药品检验研究院山东济南250101

目的考察黄连配方颗粒与标准汤剂化学成分的一致性。方法制备18批标准汤剂,测定出膏率及木兰花碱、药根碱、非洲防己碱、表小檗碱、黄连碱、巴马汀、小檗碱含量和转移率,建立HPLC特征图谱,进行聚类分析。结果18批标准汤剂和24批配方颗... 详细信息

目的考察黄连配方颗粒与标准汤剂化学成分的一致性。方法制备18批标准汤剂,测定出膏率及木兰花碱、药根碱、非洲防己碱、表小檗碱、黄连碱、巴马汀、小檗碱含量和转移率,建立HPLC特征图谱,进行聚类分析。结果18批标准汤剂和24批配方颗粒特征图谱中有11个共有峰,相似度0.861~1.000,聚为2类。配方颗粒出膏率及指标成分含量和转移率均与标准汤剂接近。结论黄连配方颗粒与标准汤剂化学成分的一致性良好,可为前者质量控制、工艺研究及临床应用提供参考。

关键词：黄连配方颗粒标准汤剂 HPLC特征图谱聚类分析

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甘油对丝素蛋白膜水溶性和结构的影响

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高等学校化学学报 2011年第10期32卷 2460-2464页

作者：罗敏清卢神州现代丝绸国家工程实验室苏州大学纺织与服装工程学院苏州215123

探讨了甘油的加入对丝素蛋白溶解过程的结晶结构及结晶度的影响.以甘油为添加剂用流延法于室温制备一系列丝素共混膜,测试了其含水率、溶失率及结构和机械性能.结果表明,随着甘油加入量的增加,丝素蛋白的溶失率逐渐降低;当甘油/丝素质... 详细信息

探讨了甘油的加入对丝素蛋白溶解过程的结晶结构及结晶度的影响.以甘油为添加剂用流延法于室温制备一系列丝素共混膜,测试了其含水率、溶失率及结构和机械性能.结果表明,随着甘油加入量的增加,丝素蛋白的溶失率逐渐降低;当甘油/丝素质量分数超过10%时,共混膜呈现水不溶性.当甘油加入量较少时,丝素蛋白呈现少量的SilkⅡ结晶,而SilkⅠ结构不明显.随着甘油含量的不断增加,SilkⅠ结晶逐渐增加而SilkⅡ结晶逐渐减少.当甘油加入量达到不溶点(10%)时,丝素蛋白主要转变为SilkⅠ结晶,而几乎没有SilkⅡ结晶.甘油的加入可使共混膜的柔韧性显著提高,并促使丝素蛋白结晶度提高以及促使丝素蛋白向SilkⅠ结晶转变,从而降低丝素蛋白膜的水溶性.

关键词：甘油丝素蛋白膜结晶结构结晶度机械性能

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Characterics of upper digestive tract diseasesin fishermen of the Bohai Bay

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World Journal of Gastroenterology 1997年第3期3卷 43-45页

作者： WANG yuan Ben 1, WANG yuan Ping 1, ZOU Jing 1, BAI Bao Jie 2, REN Guo Chun 2 and CAI Ben qing 2

AIM To study the characteristics of upper digestive tract diseases (UDTDs) in the fishermen of the Bohai Bay. METHODS An investigation was carried out in 1488 fishermen with symptoms of UDTDs except liver, biliary and pancreatic diseases during the period from December 1991 to February 1995, which included medical history inquiry, physical, gastroscopic and pathological examinations, detection of Helicobacter pylori ( H. pylori) infection, and nitrate content in their drinking water. RESULTS Among the 1488 subjects investigated, 1467 suffered from one or more diseases among 14 kinds of UDTDs, most of which were chronic atrophic gastritis (CAG, 1103 cases), peptic ulcer (268 cases), and cancer of the upper digestive tract (25 cases). CONCLUSION Incidence rate of UDTDs tends to be high among the fishermen, especially CAG, peptic ulcer and upper digestive tract cancer, and complicated state of illness due to their special life habits, and high nitrate content in their drinking water, etc, and UDTDs in fishermen is significantly different from that in inland residents in clinical manifestations.

关键词： digestive tract disease gastroscopy nitrate Helicobacter pylori gastritis, atrophic peptic ulcer digestive system neoplasms

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