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Natural Variation of Pto and Fen Genes and Marker-Assisted Selection for Resistance to Bacterial Speck in Tomato

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Agricultural Sciences in China 2011年第6期10卷 827-837页

作者： SUN Wan-yu ZHAO Wan-ying WANG yuan-yuan PEI cheng-cheng YANG Wen-cai Department of Vegetable Science College of Agronomy and Biotechnology China Agricultural University Beijing 100193 P.R.China

The resistance in tomato plants to bacterial speck caused by Pseudomonas syringae pv. tomato is triggered by the interactions between the plant resistance protein Pto and the pathogen avirulence proteins AvrPto or AvrPtoB. Fen is a gene encoding closely related functional protein kinases as the Pto gene. To investigate the status of resistance to the pathogen and natural variation of Pto and Fen genes in tomato, 67 lines including 29 growing in China were subject to disease resistance evaluation and fenthion-sensitivity test. Alleles of Pto and Fen were amplified from genomic DNA of 25 tomato lines using polymerase chain reaction （PCR） and sequences were determined by sequencing the PCR products. The results indicated that none of the 29 cultivars/hybrids growing in China were resistant to bacterial speck race 0 strain DC3000. Seven of eight tomato lines resistant to DC3000 were also fenthion-sensitive. Analysis of deduced amino acid sequences identified three novel residue substitutions between Pto and pto, and one new substitution identified between Fen and fen. A PCR-based marker was developed and successfully used to select plants with resistance to DC3000.

关键词： tomato bacterial speck Pto Fen natural variation marker-assisted selection

Proteomics of serum exosomes identified fibulin-1 as a novel biomarker for mild cognitive impairment

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Neural Regeneration Research 2023年第3期18卷 587-593页

作者： Bo Chen Li Song Juan Yang Wei-Ying Zhou yuan-yuan cheng Yu-Jie Lai College of Pharmacy Chongqing Medical UniversityChongqingChina Department of Neurology the First Affiliated Hospital of Chongqing Medical UniversityChongqing Key Laboratory of Major Neurological and Mental DisordersChongqing Key Laboratory of NeurologyChongqingChina Department of Neurology The Third Affiliated Hospital of Chongqing Medical UniversityChongqingChina

Mild cognitive impairment(MCI)is a prodrome of Alzheimer’s disease *** impairment patients often have a delayed diagnosis because there are no early symptoms or conventional diagnostic *** play a vital role in cell-to-cell communications and can act as promising biomarkers in diagnosing *** study was designed to identify serum exosomal candidate proteins that may play roles in diagnosing *** spectrometry coupled with tandem mass tag approach-based non-targeted proteomics was used to show the differentially expressed proteins in exosomes between MCI patients and healthy controls,and these differential proteins were validated using immunoblot and enzyme-linked immunosorbent *** of cognitive performance with the serum exosomal protein level was *** tracking analysis suggested that there was a higher serum exosome concentration and smaller exosome diameter in individuals with MCI compared with healthy *** identified 69 exosomal proteins that were differentially expressed between MCI patients and healthy controls using mass spectrometry ***-nine exosomal proteins were upregulated in MCI patients compared with those in control *** fibulin-1,with an area under the curve value of 0.81,may be a biomarker for an MCI *** exosomal protein signature from MCI patients reflected the cell adhesion molecule *** particular,higher exosomal fibulin-1 levels correlated with lower cognitive ***,this study revealed that exosomal fibulin-1 is a promising biomarker for diagnosing MCI.

关键词： Alzheimer’s disease biomarker diagnosis exosomes fibulin mass spectrometry mild cognitive impairment tandem mass tag cell adhesion molecule nanoparticle tracking analysis

Electrolyte composition and galvanic corrosion for ruthenium/copper electrochemical mechanical polishing

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Rare Metals 2020年第11期39卷 1300-1306页

作者： Yan-Fei Bian Wen-Jie Zhai yuan-yuan cheng Bao-Quan Zhu School of Mechatronics Engineering Harbin Institute of TechnologyHarbin150001China School of Civil Engineering Harbin Institute of TechnologyHarbin150001China

Electrochemical mechanical polishing(ECMP)is a new and highly promising technology.A specific challenge for integrating Ru as barrier in Cu interconnect structures is the galvanic corrosion of Cu that occurs during *** mitigate the problem,the benzotriazole(BTA)and ascorbic acid(AA)were chosen as selective anodic and cathodic inhibitors for Cu and Ru,*** optimization of electrolytes at different pHs including BTA,hydroxyethylidenediphosphoric acid(HEDP),and AA were investigated using electrochemical *** Ru/Cu removal rate and the planarization efficiency during Ru/Cu ECMP can be approximated using electrochemical measurements of the removal rate,with and without surface *** systems that exhibit a 1:1 selectivity between the barrier layer and copper would be ideal for the barrier removal step of *** slurry consists of 20.0 wt%HEDP,0.5 wt%BTA,and 0.3 wt%AA at pH *** the optimized slurry,the selectivity of Ru to Cu is near *** measurements of open circuit potentials,potentiodynamic polarization,and impedance spectroscopy were performed to investigate the galvanic corrosion between ruthenium and copper.

关键词： Electrochemical mechanical polishing Electrolyte composition Galvanic corrosion Benzotriazole Ascorbic acid

β-Carboline alkaloids from Trigonostemon filipes and Trigonostemon lii

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Natural Products and Bioprospecting 2012年第3期2卷 126-129页

作者： Shi-Fei LI yuan-yuan cheng Yu ZHANG Shun-Lin LI Hong-Ping HE Xiao-Jiang HAO State Key Laboratory of Phytochemistry and Plant Resources in West China Kunming Institute of BotanyChinese Academy of SciencesKunming 650201China Graduate University of Chinese Academy of Sciences Beijing 100049China

Sixβ-carboline alkaloids including three new ones,trifilines A-C(1-3)were isolated from Trigonostemon filipes,along with a newβ-carboline alkaloid,trigonoine C(7)isolated from Trigonostemon *** structures were elucidated by extensive spectroscopic *** A(1)and B(2)showed weak anti-HIV-1 activity with EC50 values of 54.61μg/mL and 9.75μg/mL,along with TI(Therapeutic index)values of 1.52 and 1.42,*** is the first time to report the chemical constituents of Trigonostemon filipes.

关键词： Trigonostemon filipes Trigonostemon lii β-carboline alkaloids trifilines A-C trigonoine C

Abundant Production of Reactive Water Radical Cations under Ambient Conditions

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CCS Chemistry 2022年第4期4卷 1224-1231页

作者： Meng Wang Xiao-Fei Gao Rui Su Peng He yuan-yuan cheng Ke Li Dongbo Mi Xiaoping Zhang Xinglei Zhang Huanwen Chen R.Graham Cooks Jiangxi Key Laboratory for Mass Spectrometry and Instrumentation East China University of TechnologyNanchang 330013 State Key Laboratory of Inorganic Synthesis and Preparative Chemistry College of ChemistryJilin UniversityChangchun 130012 Department of Chemistry and Center for Analytical Instrumentation Development Purdue UniversityWest LafayetteIN 47907

Water radical cations,the crucial intermediates in many aqueous reactions and biochemical processes,are difficult to investigate experimentally due to their short lifetime ***,ahomemade device based on energy-tunable discharge was employed to deposit suitable amounts of energy to atmospheric pressure pure water vapor for abundant production of water radical cations,stabilized as(H_(2)O)n^(+·)(n=2-5)with a maximal abundance of≥8.3×10^(6) cps for(H_(2)O)_(2)^(+·),characterized by mass spectrometry(MS).The abundance of water radical cations was optimized by adjusting the experimental parameters such as the discharge voltage(2.5 kV),temperature of the MS inlet(140℃),carrier gas flow(20mL/min),and the distance between the discharge tip and MS inlet(12 mm).The ambient formation of water radical cationswas further confirmedby thehigh reactivity of as-prepared water radical cations,which reacted with benzene,ethyl acetate,and dimethyl disulfide instantly,showing rich chemistry with ionic and radical ***,the computations usingCCSD(T)//MP2 method and density functional theory confirmed that the O-O single-electron bound dimer(B),as well as the hydronium hydroxyl radical complex(A),accounted for the unusual chemistry of the water radical cations,providing a facile approach to access the high reactivity ofwater radical cations under the ambient conditions.

关键词： water radical cation mass spectrometry O-O single-electron bound dimer of water radical cation hydronium hydroxyl dimer of water radical cation

Tuning the color of firefly bioluminescence: a theoretical study on luciferin and luciferase modifications

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Tuning the color of firefly bioluminescence: a theoretical s...

Color-Tuning of the firefly oxyluciferin analogs investigated by Hybrid QM/MM simulations

第十三届全国量子化学会议

作者： yuan-yuan cheng Ya-Jun Liu Key Laboratory of Theoretical and Computational Photochemistry Ministry of Education College of ChemistryBeijing Normal University

Firefly bioluminescence has various applications, such as biological analysis. To enhance the detection sensitivity and extend the application scope, a wide range of bioluminescence color is required. Both luciferin and luciferase modifications can achieve this goal. Experimentally,several luciferin analogues have been synthesized[1,2], but a modified regulation has not been proposed. Theoretically, we investigate the spectral properties of series of oxyluciferin analogues in the gas phase by time-dependent density functional theory（TD DFT）. A systematic modified regulation is summarized and one oxyluciferin analogue emitting near-infrared light is suggested. Massive mutants of luciferase have been reported at experiment[3,4], but theoretical study on mutant is scarce. We study the spectral properties of oxyluciferin（keto-1 form） and suggested oxyluciferin analogue in four mutants（S284T, R218 A, R337 K and PpyLit[5]） by hybrid quantum mechanics/molecular mechanics（QM/MM） combined with molecular dynamics（MD） method. A high accuracy method, multistate complete active space second-order perturbation（MS-CASPT2）, is employed. The steric, electrostatic, and H-bond effects are discussing.

关键词： modification luciferin luciferase bioluminescence

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Color-Tuning of the firefly oxyluciferin analogs investigate...

Expression and clinical significance of serum PCT and MIF in patients with sepsis

中国化学会第29届学术年会

作者： yuan-yuan cheng Ya-Jun Liu College of Chemistry Beijing Normal University

To improve the visualization of living cells,extend the firefly bioluminescence is of practical *** modification of luciferase and luciferin are the two main aspects of tuning the color of firefly *** present,we focus on the study of luciferin *** theory study of oxyluciferin analogs’emission spectra in gas and in solutions has been reported.1 In this work,we study the firefly bioluminescence spectra of nine synthesized oxyluciferin analogs,some of which have been reported2,in protein by hybrid quantum mechanics/molecular mechanics（QM/MM）*** results are compared with that of the phenolate-keto-oxyluciferin,which is the only light emitter of *** on the calculated results,the H bond effects,rotation effects,andπ-πstacking effects are discussing.

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Expression and clinical significance of serum PCT and MIF in...

Chinese herbal medicine Xinfeng Capsule in treatment of rheumatoid arthritis:study protocol of a multicenter randomized controlled trial

中华医学会呼吸病学年会——2013（第十四次全国呼吸病学学术会议）

作者： yuan yuan cheng Jie Zhang Yu Biao Guo Pulmonary and Critical Care Medicine the First Affiliated Hospital of Sun Yat-Sen UniversityGuangzhou Intensive Care Unit the First Peoples HospitalGangZhou Intensive Care Unit Guangzhou institute of respiratory diseases of Guangzhou Medical University

Objective To investigate the expression and clinical siginificance of procalcitonin(PCT) and macrophage migration inhibitory factor(MIF) for diagnostic efficacy, severity stratification and prognostic outcome of sepsis. Methods ninety sepsis patients admitted to ICU were prospectively divided into the sepsis group(n=18),the severe sepsis group(n=40) and the septic shock group (n=32).The serum PCT was measured by ECLIA,and the serum level of MIF was measured by ***

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Journal of Integrative Medicine 2013年第6期11卷 428-434页

作者： Jian Liu Chuan-bing Huang yuan Wang Gui-qin Xu yuan-yuan cheng Yun-xia Feng Lei Liu Ya-jun Qi Department of Rheumatology the First Affiliated HospitalAnhui University of Chinese Medicine

BACKGROUND： Rheumatoid arthritis （RA）, as a common systemic inflammatory autoimmune disease, affects approximately 1 in 100 individuals. Effective treatment for RA is not yet available because current research does not have a clear understanding of the etiology and pathogenesis of RA. Xinfeng Capsule, a patent Chinese herbal medicine, has been used in the treatment of RA in recent years. Despite its reported clinical efficacy, there are no large-sample, multicenter, randomized trials that support the use of Xinfeng Capsule for RA. Therefore, we designed a randomized, double-blind, multicenter, placebo-controlled trial to assess the efficacy and safety of Xinfeng Capsule in the treatment of RA. METHODS AND DESIGN： This is a 12-week, randomized, placebo-controlled, double-blind, multicenter trial on the treatment of RA. The participants will be randomly assigned to the experimental group and the control group at a ratio of 1：1. Participants in the experimental group will receive Xinfeng Capsule and a pharmaceutical placebo （imitation leflunomide）. The control group will receive leflunomide and an herbal placebo （imitation Xinfeng Capsule）. The American College of Rheumatology （ACR） Criteria for RA will be used to measure the efficacy of the Xinfeng Capsule. The primary outcome measure will be the percentage of study participants who achieve an ACR 20% response rate （ACR20）, which will be measured every 4 weeks after randomization. Secondary outcomes will include the ACR50 and ACR70 responses, the side effects of the medications, the Disease Activity Score 28, RA biomarkers, quality of life, and X-rays of the hands and wrists. The first four of the secondary outcomes will be measured every 4 weeks and the others will be measured at baseline and after 12 weeks of treatment. DISCUSSION： The result of this trial will help to evaluate whether Xinfeng Capsule is effective and safe in the treatment of RA. TRIAL REGISTRATION： This trial has been registered in ClinicalTrials.g

关键词： Xinfeng Capsule rheumatoid arthritis double-blind method placebos ACRcriteria quality of life randomized controlled trials clinical protocols