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Integrated network analysis of transcriptomic and protein-protein interaction data in taurine-treated hepatic stellate cells

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World Journal of Gastroenterology 2019年第9期25卷 1067-1079页

作者： xing-Qiu Liang Jian Liang Xiao-Fang Zhao xin-yuan wang xin Deng Department of Science and Technology Ruikang Hospital Affiliated to Guangxi University of Chinese Medicine College of Medical Guangxi University School of Basic Sciences Guangxi University of Chinese Medicine

BACKGROUND Studies show that the antifibrotic mechanism of taurine may involve its inhibition of the activation and proliferation of hepatic stellate cells(HSCs). Since the molecular mechanism of taurine-mediated antifibrotic activity has not been fully unveiled and is little studied, it is imperative to use "omics" methods to systematically investigate the molecular mechanism by which taurine inhibits liver *** To establish a network including transcriptomic and protein-protein interaction data to elucidate the molecular mechanism of taurine-induced HSC *** We used microarrays, bioinformatics, protein-protein interaction(PPI) network,and sub-modules to investigate taurine-induced changes in gene expression in human HSCs(LX-2). Subsequently, all of the differentially expressed genes(DEGs) were subjected to gene ontology function and Kyoto encyclopedia of genes and genomes pathway enrichment analysis. Furthermore, the interactions of DEGs were explored in a human PPI network, and sub-modules of the DEGs interaction network were analyzed using Cytoscape *** A total of 635 DEGs were identified in taurine-treated HSCs when compared with the controls. Of these, 304 genes were statistically significantly up-regulated, and 331 down-regulated. Most of these DEGs were mainly located on the membrane and extracellular region, and are involved in the biological processes of signal transduction, cell proliferation, positive regulation of extracellular regulated protein kinases 1(ERK1) and ERK2 cascade, extrinsic apoptotic signaling pathway and so on. Fifteen significantly enriched pathways with DEGs were identified, including mitogen-activated protein kinase(MAPK) signaling pathway, peroxisome proliferators-activated receptor signaling pathway,estrogen signaling pathway, Th1 and Th2 cell differentiation, cyclic adenosine monophosphate signaling pathway and so on. By integrating the transcriptomics and human PPI data, nine critical genes, inclu

关键词： Taurine Hepatic stellate cells Differentially expressed genes Liver fibrogenesis Transcriptomic Protein-protein interaction network

Heat sensitivity of eggs attributes to the reduction in Agasicles hygrophila population

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Insect Science 2020年第1期27卷 159-169页

作者： Dong Jia Xiao-Fang yuan Yan-Hong Liu Chao-Qian Xu yuan-xin wang Ling-Ling Gao Rui-Yan Ma College of Agriculture Shanxi Agricultural UniversityTaiguShanxiChina College o f Life Science Shanxi Agricultural UniversityTaiguShanxiChina CSIRO Agriculture and Food Centre for Environment and Life SciencesWembleyWestern AustraliaAustralia

Agasicles hygrophila has been introduced worldwide as a control agent for the invasive weed Alternanthera ***,global warming has potential impact on its controlling *** aim of this research was to explore the primary factors responsible for the greatly reduced *** population in hot *** imitate the temperature conditions in summers,different developmental stages of hygrophila were treated with high temperatures from 32.5℃ to 45℃ for 1-5 *** on the survival rate,the heat tolerance of each developmental stage was ranked from lowest to highest as follows:egg,1st,2nd,3rd instar larva,adult and *** showed the lowest heat tolerance with 37.5℃ as the critical temperature affecting larval *** treatment of the *** eggs at 37.5℃ for 1 h decreased the hatch rate to 24%.Our results indicated that when compared with the control at 25℃,1 h treatment at 37.5℃ prolonged the duration of the egg stage,shortened the duration of oviposition and total longevity,and changed the reproductive pattern of *** net reproductive rate,intrinsic rate and finite rate were all significantly *** results suggest that low heat tolerance of the eggs was the major factor responsible for the reduction of *** populations,and the key temperature was 37.5°***,appropriate measures should be taken to protect eggs in order to maintain the efficacy of *** in the biological control of *** in hot summers.

关键词： age-stage two-sex life table alligator weed flea beetle heat tolerance population dynamics

Synthesis and Tumor Cytotoxicity of Novel N-Substituted Glucosamine-bearing Oleanolic Acid Derivatives

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Chemical Research in Chinese Universities 2014年第4期30卷 639-643页

作者： REN Li LIU Yang YU Guihua GAO yuan LIU xin wang Bo DENG Xiaonan CHENG Maosheng Key Laboratory of Structure-based Drug Design and Discovery Ministry of Education Shenyang Pharmaceutical University Shenyang 110016 P. R. China

Eleven novel triterpenoid saponins,N-substituted-β-D-glucosaminide derivatives of oleanolic acid,were designed and synthesized via a stepwise glycosylation *** compounds were evaluated for in vitro cytotoxic activity against six different tumor cell *** of the compounds inhibited the growth of,at least,one tumor cell line effectively at micromolar *** structure-activity relationships（SARs） indicate that acylation of the nitrogen of the glucosamine-bearing triterpenoid saponins affords the compounds that are highly cytotoxic towards specific tumor cell lines.

关键词： N-Substituted glucosamine-bearing triterpenoid saponin Tumor cytotoxicity Oleanolic acid

5’tiRNA-Pro-TGG,a novel tRNA halve,promotes oncogenesis in sessile serrated lesions and serrated pathway of colorectal cancer

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World Journal of Gastrointestinal Oncology 2023年第6期15卷 1005-1018页

作者： xin-yuan wang Yu-Jie Zhou Hai-Ying Chen Jin-Nan Chen Shan-Shan Chen Hui-Min Chen Xiao-Bo Li Division of Gastroenterology and Hepatology Renji HospitalShanghai Jiaotong University School of MedicineShanghai 200000China Department of Spleen and Stomach and Rheumatology Affiliated Traditional Chinese Medicine Hospital of Southwest Medical UniversityLuzhou 646000Sichuan ProvinceChina

BACKGROUND Transfer RNA(tRNA)-derived small RNAs(tsRNAs)are small fragments that form when tRNAs *** halves(tiRNAs),a subcategory of tsRNA,are involved in the oncogenic processes of many ***,their specific role in sessile serrated lesions(SSLs),a precancerous lesion often observed in the colon,has not yet been *** To identify SSL-related tiRNAs and their potential role in the development of SSLs and serrated pathway of colorectal cancer(CRC).METHODS Small-RNA sequencing was conducted in paired SSLs and their adjacent normal control(NC)*** expression levels of five SSL-related tiRNAs were validated by q-polymerase chain *** counting kit-8 and wound healing assays were performed to detect cell proliferation and *** target genes and sites of tiRNA-1:33-Pro-TGG-1(5′tiRNA-Pro-TGG)were predicted by TargetScan and miRanda ***-associated and immune-related pathways were analyzed by single-sample gene set enrichment *** analyses were performed to establish the roles of 5′tiRNA-Pro-TGG based on the target *** In total,we found 52 upregulated tsRNAs and 28 downregulated tsRNAs in SSLs compared to *** expression levels of tiRNA-1:33-Gly-CCC-2,tiRNA-1:33-Pro-TGG-1,and tiRNA-1:34-Thr-TGT-4-M25′tiRNAs were higher in SSLs than those in NC,while that of 5′tiRNA-Pro-TGG was associated with the size of *** was demonstrated that 5′tiRNAPro-TGG promoted cell proliferation and migration of RKO cell in ***,heparanase 2(HPSE2)was identified as a potential target gene of 5′*** lower expression was associated with a worse prognosis in ***,lower expression of HPSE2 was observed in SSLs compared to normal controls or conventional adenomas and in BRAF-mutant CRC compared to BRAF-wild *** analyses revealed that its low expression was associated with a low interferonγresponse and also with many metabolic pathways such as riboflavin,retinol,and cy

关键词： Noncoding RNA tRNA halves Sessile serrated lesions Colon cancer Serrated pathway

EXPERIMENTAL STUDY ON ENHANCED HEAT TRANSFER WITH NI-BASED IMPLANTED SPIRAL FINNED TUBES

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Journal of Hydrodynamics 2003年第5期15卷 65-69页

作者： Sun Feng zhong, Huang xin yuan, wang Nai hua, Shi Yue tao, Ping Ya ming School of Energy and Power Engineering, Shandong University, Jinan 250061, China Sch. of Energy and Power Eng. Shandong Univ. Ji'nan 250061 China

The new type of heat exchanger elements-Ni-based Implanted Spiral FinnedTurbes (NISFT) was studied and tested, which can contribute to increase the efficiency andreliability. The relation of Nu, Eu and Re with different fin pitch, fin height, transverse pitchand longitudinal pitch were gained, which constituted the basic foundation for the engineeringapplication of NISFT.

关键词： enhanced heat transfer heat exchanger finned tube

LC-ESI-MS/MS,a modified method for simultaneous quantification of isoflavonoids,flavonoids,alkaloids and saponins in a Chinese herbal preparation Gegen-Qinlian decoction

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Journal of Pharmaceutical Analysis 2010年第3期22卷 141-148页

作者： Ying wang,Jin yuan,Rui An,xin-Hong wang,Yue-Ming Ma Department of Chemistry,Shanghai University of Traditional Chinese Medicine School of Pharmacy,Shanghai 201203,China Department of Chemistry Shanghai University of Traditional Chinese Medicine School of Pharmacy Shanghai 201203 China

A sensitive and reliable liquid chromatography-electrospray ionisation-tandem mass spectrometry(LC-ESI-MS/MS)method was established to simultaneously quantitate four categories of compounds(isoflavonoids,flavonoids,alkaloids and saponins)in Gegen-Qinlian decoction(GQD).These compounds were separated by a Shiseido CAPCELL PAK C18 column with a linear gradient consisting of 0.1%(v/v)formic acid in water(A)and 0.1%(v/v)formic acid in acetonitrile(B),and delivered at a flow rate of 0.3 mL/*** the analytes were determined by electrospray positive ionization tandem mass spectrometry in a multiple reaction monitoring(MRM)***,accuracy,precision,recovery and stability of the method were evaluated with the validation over the range of 4.0-538 5 ng/*** proposed method was applied to the analysis of a Chinese herbal preparation GQD successfully.

关键词： Gegen-Qinlian decoction(GQD) liquid chromatography-electrospray ionization-tandem mass spectrometry(LC-ESI-MS/MS) quantification

Repair of left ventricular aneurysm： ten-year experience in Chinese patients

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Chinese Medical Journal 2009年第17期122卷 1963-1968页

作者： FAN Hong-guang ZHENG Zhe FENG Wei yuan xin wang Wei HU Sheng-shou Department of Cardiovascular Surgery Center for CardiovascularRegenerative Medicine Fu Wai Hospital Peking Union MedicalCollege and Chinese Academic of Medical Sciences Beijing100037 China

Background A large transmural myocardial infarction often results in a dyskinetic or akinetic left ventricular aneurysm （LVA）. This study aimed to explore the early and long-term clinical outcomes and to identify predictors for survivals and hospital re-admission after the repair of left ventricular aneurysm. Methods We followed up 497 patients who had undergone LVA repair from a single center in China between 1995 and 2005. The perioperative parameters were recorded. Risk factors for early mortality and long-term results were analyzed by multivariate Logistic regression. Cox＇s proportional hazard model was used to calculate risk factors for major adverse cardiac and cerebrovascular events, cause of death and re-admission. Kaplan-Meier curve was employed to analyze long-term survival. Results The operative mortality was 2.0%. The long-term mortality was 11.1% and cardiac causes contributed to 61.8% of the overall long-term mortality. Four hundred and thirty-two patients survived during the follow-up period and 37.5% of them had been re-admitted at least one time. One hundred and five patients experienced major adverse cardiac and cerebrovascular events. Survival analysis exhibited that the probability of survival at 1 and 5 years after operation was 96% and 86% respectively. Previous atrial fibrillation was the independent risk factor for early mortality. Independent risk factors for long-term mortality were poor left ventricular ejection fraction and stroke, and risk factors for cardiac mortality were intraventricular block, stroke and poor left ventricular ejection fraction. Stroke, intraventricular block and advanced age were independent risk factors for major adverse cardiac and cerebrovascular events, and New York Heart Association （NYHA） class Ill-IV was the only risk factor for hospital re-admission. Conclusions Postinfarction LVA can be repaired and satisfying early and long-term clinical outcome can be obtained. Endoventricular circular plasty technique is

关键词： heart aneurysm surgical procedures, operative follow-up studies risk factors outcome assessment

Breeding of Brassica napus Cultivar Zhongshuang9 with High-Resistance to Sclerotinia sclerotiorum and Dynamics of Its Important Defense Enzyme Activity

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Agricultural Sciences in China 2003年第11期2卷 1192-1197页

作者： wang Han-zhong, LIU Gui-hua, ZHENG yuan-ben, wang xin-fa and YANG Qing(Institute of Oil Crops Research , Chinese Academy of Agricultural Sciences , Wuhan 430062 , P. R. China) Institute of Oil Crops Research Chinese Academy of Agricultural Sciences Wuhan 430062 P. R. China

Zhongshuang9, a new semi-winter Brassica napus variety with high resistance to Sclerotinia sclerotiorum and lodging, high-yield, double-low quality and extensive adaptability, was bred by multiple crossing and microspore culture technique. It was registered and released in China in 2002. In regional trial of Hubei Province in China, Zhongshuang9 yielded 2 482. 2 kg ha-1 averagely in 2000 - 2002, 15. 33% higher than the control variety Zhongyou821. Erucic acid, glucosinolates and oil contents of Zhongshuang9 were 0.23%, 22.69 μmol g-1(in meal)and 42%, respectively. In field assessment of resistance to S. Sclerotiorum , the disease incidence and disease index of Zhongshuang9 averaged 13.31 % and 6.47, respectively, which were lower than those of Zhongyou821 by 28% and 36%, respectively. After inoculation of detached leaves with mycelia, the lesion size of Zhongshuang9 was 4. 709 cm2, which was significantly smaller than that of the mid-resistant variety Zhongyou821(5. 933 cm2). The stem lesion length of Zhongshuang9 after match-stick inoculation was 1.275 cm, which was significantly lower than that of Zhongyou821(1.943 cm). The possible mechanism of resistance to S. sclerotiorum was studied through comparing the activities of phenylalanine ammonia lyase(PAL), exo-chitinase, β-1, 3-glucanase, peroxidase(POD)and polyphenoloxidase(PPO)in Zhongshuang9 with those in other resistant, mid-resistant and susceptible cultivars.

关键词： Brassica napus Zhongshuang9 Resistance to Sclerotinia sclerotiorum Double-low Defense enzyme Dynamics

Motor Driving Leg Design for Bionic Crab-like Robot

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哈尔滨工程大学学报（英文版） 2002年第2期1卷 72-77页

作者： wang Li-quan, Qu Xiao-rong, Meng Qing-xin, yuan Peng, wang Mo-nanCollege of Mechanical and Electrical Engineering, Harbin Engineering University, Harbin 150001, China College of Mechanical and Electrical Engineering Harbin Engineering University Harbin China

The paper presents the design of walking leg for bionics crab-like robot, which is driven with micro servo motor. The kinematic characteristics of the bionics machine are analysed for optimized structure parameters, which has been used in the robot design. A three closed loop motor control system structure for joint driver is also given, as well as the multi-joint driving system for walking robot leg.

关键词： bionics crab-liked robot joint driver servo motor three-closed-loop