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Protein-protein Interaction Between Domains of PDZ and BAR from PICK1

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Chemical Research in Chinese Universities 2007年第2期23卷 191-195页

作者： XIAO Hong SHI Ya-wei WANG Li-li yuan jing-ming Institute of Biotechnology Key Laboratory of Chemical Biology and Molecular Engineering of National Ministry of Education Shanxi University Taiyuan 030006 P. R. China

Two DNA fragments encoding PDZ domain （21-110 residues） and BAR domain （ 150-360 residues） from PICK1 （1-416 residues） were amplified by PCR and then introduced into vectors, pET-32M and pMAL-e2X respectively to generate recombinant plasmids, pE-pdz and pM-bar. Having been separately transferred into the hosts E. coli BL21 and E. coli JM109, these two strains can express fusion proteins： His-tagged PDZ（PDZ domain） and maltose binding protein-BAR（ MBP-BAR domain） respectively, as confirmed by both SDS-PAGE and Wostem blotting. The interaction between these two domains is dose-dependence, as identified by a pull-down test. Moreover, it has been shown from the ELISA analysis that the actual amount of PDZ bound to MBP-BAR-amylose beads reaches （ 16 ± 0. 5）%, as calculated by the molar ratio of PDZ to MBP-BAR. In addition, the interaction between BAR（bait） and PDZ（prey） in vivo was also examined with a yeast two-hybrid system.

关键词： BAR domain PDZ domain PICK1 Protein-protein interaction Pull-down test Yeast two-hybrid

Prognostic significance of nuclear hepatoma-derived growth factor expression in gallbladder cancer

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World Journal of Gastroenterology 2014年第28期20卷 9564-9569页

作者： Feng Tao Min-Feng Ye Ai-jing Sun Jie-Qing Lv Guan-Gen Xu yuan-ming jing Wei Wang Department of Gastrointestinal Surgery Shaoxing People’s Hospital Shaoxing Hospital of Zhejiang University Department of Pathology Shaoxing People’s Hospital

AIM: To assess the expression of nuclear hepatoma-derived growth factor (HDGF) in benign and malignant gallbladder lesions and to determine its clinicopathological significance.

关键词： Hepatoma-derived growth factor Kaplan-Meier analysis Gallbladder cancer Immunohistochemistry

Dynamic Regulation of Hydrogen Bonding Networks and Solvation Structures for Synergistic Solar‑Thermal Desalination of Seawater and Catalytic Degradation of Organic Pollutants

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Nano-Micro Letters 2025年第2期17卷 548-565页

作者： ming‑yuan Yu jing Wu Guang Yin Fan‑Zhen Jiao Zhong‑Zhen Yu Jin Qu Beijing Key Laboratory of Advanced Functional Polymer Composites Beijing University of Chemical TechnologyBeijing 100029People’s Republic of China State Key Laboratory of Organic‑Inorganic Composites College of Materials Science and EngineeringBeijing University of Chemical TechnologyBeijing 100029People’s Republic of China

Although solar steam generation strategy is efficient in desalinating seawater,it is still challenging to achieve continuous solar-thermal desalination of seawater and catalytic degradation of organic ***,dynamic regulations of hydrogen bonding networks and solvation structures are realized by designing an asymmetric bilayer membrane consisting of a bacterial cellulose/carbon nanotube/Co_(2)(OH)_(2)CO_(3)nanorod top layer and a bacterial cellulose/Co_(2)(OH)_(2)CO_(3)nanorod(BCH)bottom ***,the hydrogen bonding networks inside the membrane can be tuned by the rich surface–OH groups of the bacterial cellulose and Co_(2)(OH)_(2)CO_(3)as well as the ions and radicals in situ generated during the catalysis ***,both SO_(4)^(2−)and HSO_(5)−can regulate the solvation structure of Na^(+)and be adsorbed more preferentially on the evaporation surface than Cl^(−),thus hindering the de-solvation of the solvated Na^(+)and subsequent nucleation/growth of ***,the heat generated by the solar-thermal energy conversion can accelerate the reaction kinetics and enhance the catalytic degradation *** work provides a flow-bed water purification system with an asymmetric solar-thermal and catalytic membrane for synergistic solar thermal desalination of seawater/brine and catalytic degradation of organic pollutants.

关键词： Solar steam generation Seawater desalination Catalytic degradation Bacterial cellulose Cobalt hydroxycarbonate nanorods

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我国中小企业集群的国际化

学习与探索 2006年第5期 187-190页

作者：任熹真袁晶明哈尔滨工业大学人文学院哈尔滨150001

我国中小企业集群发展和成长的出路在于国际化经营,国际化经营成功与否取决于其核心能力的增强。核心能力具有知识性、辐射型、不可仿制性的特征,核心能力的形成影响中小企业集群的行为和未来的选择。中小企业集群在国际化成长中要开拓... 详细信息

我国中小企业集群发展和成长的出路在于国际化经营,国际化经营成功与否取决于其核心能力的增强。核心能力具有知识性、辐射型、不可仿制性的特征,核心能力的形成影响中小企业集群的行为和未来的选择。中小企业集群在国际化成长中要开拓地域环境和创造适应中小企业集群国际化经营的行业环境,并在此基础上寻求适应我国中小企业集群国际化成长的途径。

关键词：中小企业集群国际化核心能力跨国并购

Catalytic Performance of Carbon Smoke over Ag-LSCF Composite Catalysts

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Journal of Wuhan University of Technology(Materials Science) 2025年第1期40卷 30-34页

作者： GUO Guanlun HAN ming LU Shaomin YU jing JU Hongling 无 Hubei Key Laboratory of Advanced Technology for Automotive Components&Hubei Collaborative Innovation Center for Automotive Components Technology Wuhan University of TechnologyWuhan 430070China College of Mechanical and Transportation Southwest Forestry UniversityKunming 650000China

To improve the catalytic performance of La_(0.6)Sr_(0.4)Co_(0.2)Fe_(0.8)O_(3)(LSCF)towards carbon soot,we utilized the impregnation method to incorporate Ag into the prepared LSCF *** conducted a series of characterization tests and evaluated the soot catalytic activity of the composite catalyst by comparing it with the LaCoO_(3) group,LaFeO_(3) group,and catalyst-free *** results indicate that the Ag-LSCF composite catalyst exhibits the highest soot catalytic activity,with the characteristic temperature values of 376.3,431.1,and 473.9℃at 10%,50%,and 90%carbon soot conversion,*** values are 24.8,20.2,and 23.1℃lower than those of the LSCF *** also shows that LSCF can improve the catalytic activity of soot after compounding with Ag,and reflects the necessity of using catalysts in soot combustion *** characterization and BET test show that Ag-LSCF has more abundant surface-adsorbed oxygen species,larger specific surface area and pore volume than LSCF,which also proves that Ag-LSCF has higher soot catalytic activity.

关键词： metallic composites carbon smoke oxidation perovskite catalyst soot

改进的小波神经网络算法对变流器的故障诊断方法

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计算机应用 2011年第8期31卷 2143-2145页

作者：段其昌张亮袁景明重庆大学自动化学院重庆400030

变流器是双馈风力发电系统中的枢纽设备,其运行可靠性直接关系到发电系统的安全与稳定。针对基于递推最小二乘(RLS)算法的离散小波神经网络(DWNN)存在收敛速度慢、收敛精度不高、搜索局部极小等不足,以变流器的电流为分析对象,提出一种... 详细信息

变流器是双馈风力发电系统中的枢纽设备,其运行可靠性直接关系到发电系统的安全与稳定。针对基于递推最小二乘(RLS)算法的离散小波神经网络(DWNN)存在收敛速度慢、收敛精度不高、搜索局部极小等不足,以变流器的电流为分析对象,提出一种采用变加权和变学习率改进算法的小波神经网络的变流器故障诊断方法。选择变流器电流作为离散小波神经网络训练及故障识别样本,对训练过程和仿真结果进行对比分析。实验结果表明:较之RLS算法,改进的小波神经网络故障诊断方法在故障识别准确率和收敛时间方面表现更优。

关键词：变流器故障诊断离散小波神经网络递推最小二乘法变加权变学习率

Progress of research in the application of ultrasound technology for the treatment of Alzheimer’s disease

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Neural Regeneration Research 2025年第10期20卷 2823-2837页

作者： Qiuquan Cai Lianghui Meng Meina Quan Ling Wang jing Ren Chenguang Zheng Jiajia Yang Dong ming Academy of Medical Engineering and Translational Medicine Tianjin UniversityTianjinChina Innovation Center for Neurological Disorders Xuanwu HospitalCapital Medical UniversityBeijingChina Brain-Computer Interaction and Human-Machine Fusion Haihe Laboratory Tianjin UniversityTianjinChina Tianjin Key Laboratory of Brain Science and Neural Engineering Tianjin UniversityTianjinChina

Alzheimer’s disease is a common neurodegenerative disorder defined by decreased reasoning abilities,memory loss,and cognitive *** presence of the blood-brain barrier presents a major obstacle to the development of effective drug therapies for Alzheimer’s *** use of ultrasound as a novel physical modulation approach has garnered widespread attention in recent *** a safe and feasible therapeutic and drug-delivery method,ultrasound has shown promise in improving cognitive *** article provides a summary of the application of ultrasound technology for treating Alzheimer’s disease over the past 5 years,including standalone ultrasound treatment,ultrasound combined with microbubbles or drug therapy,and magnetic resonance imaging-guided focused ultrasound *** is placed on the benefits of introducing these treatment methods and their potential *** found that several ultrasound methods can open the blood-brain barrier and effectively alleviate amyloid-βplaque *** believe that ultrasound is an effective therapy for Alzheimer’s disease,and this review provides a theoretical basis for future ultrasound treatment methods.

关键词： Alzheimer’s disease blood-brain barrier drugs magnetic resonance imaging-guided focused ultrasound microbubbles scanning ultrasound ultrasound ultrasound stimulation

FUBP3 mediates the amyloid-β-induced neuronal NLRP3 expression

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Neural Regeneration Research 2025年第7期20卷 2068-2083页

作者： jing Yao yuan Li Xi Liu Wenping Liang Yu Li Liyong Wu Zhe Wang Weihong Song The National Clinical Research Center for Geriatric Disease Xuanwu HospitalCapital Medical UniversityBeijingChina Department of Neurology The First Affiliated Hospital of Zhengzhou UniversityZhengzhouHenan ProvinceChina Department of Neurosurgery Xuanwu HospitalCapital Medical UniversityBeijingChina Department of Pediatrics The Second Affiliated Hospital and Yuying Children’s HospitalKey Laboratory of Alzheimer’s Disease of Zhejiang ProvinceInstitute of AgingWenzhou Medical UniversityWenzhouZhejiang ProvinceChina Oujiang Laboratory(Zhejiang Laboratory for Regenerative Medicine Visionand Brain Health)WenzhouZhejiang ProvinceChina

Alzheimer's disease is characterized by deposition of amyloid-β,which forms extracellular neuritic plaques,and accumulation of hyperphosphorylated tau,which aggregates to form intraneuronal neurofibrillary tangles,in the *** NLRP3 inflammasome may play a role in the transition from amyloid-βdeposition to tau phosphorylation and *** NLRP3 is primarily found in brain microglia,and tau is predominantly located in neurons,it has been suggested that NLRP3 expressed by microglia indirectly triggers tau phosphorylation by upregulating the expression of pro-inflammatory ***,we found that neurons also express NLRP3 in vitro and in vivo,and that neuronal NLRP3 regulates tau *** biochemical methods,we mapped the minimal NLRP3 promoter and identified FUBP3 as a transcription factor regulating NLRP3 expression in *** primary neurons and the neuroblastoma cell line Neuro2A,FUBP3 is required for endogenous NLRP3 expression and tau phosphorylation only when amyloid-βis *** the brains of aged wild-type mice and a mouse model of Alzheimer's disease,FUBP3 expression was markedly increased in cortical *** analysis suggested that FUBP3 plays a role in neuron-mediated immune *** also found that FUBP3 trimmed the 5′end of DNA fragments that it bound,implying that FUBP3 functions in stress-induced *** findings suggest that neuronal NLRP3 may be more directly involved in the amyloid-β-to–phospho-tau transition than microglial NLRP3,and that amyloid-βfundamentally alters the regulatory mechanism of NLRP3 expression in *** that FUBP3 was only expressed at low levels in young wild-type mice and was strongly upregulated in the brains of aged mice and Alzheimer's disease mice,FUBP3 could be a safe therapeutic target for preventing Alzheimer's disease progression.

关键词： 5′end trimming Alzheimer's disease amyloid-beta amyloid-β-dependent transcription FUBP3 inflammasome inflammation neuron NLRP3 tau transcription factor

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邓玉珠:商务王国的女纵横家

中外企业家 2008年第10期 8-15页

作者：袁晶明平明

邓玉珠女士是贯中咨询有限公司执行董事。贯中咨询有限公司是一家管理和投资咨询公司,总部位于芝加哥,在华盛顿特区设有联络处,在北京、上海和香港有合作同事。贯中咨询侧重于中美商务活动,擅长制定并执行战略、建立并管理网络以及进行... 详细信息

邓玉珠女士是贯中咨询有限公司执行董事。贯中咨询有限公司是一家管理和投资咨询公司,总部位于芝加哥,在华盛顿特区设有联络处,在北京、上海和香港有合作同事。贯中咨询侧重于中美商务活动,擅长制定并执行战略、建立并管理网络以及进行业务谈判。

关键词：玉珠纵横家银行金融机构罗耀拉大学交易员商务国家形式王国