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Chinese Chemical Letters 2014年第5期25卷 745-748页

作者： Qing-Xiang Zhou yuan-yuan gao College of Geosciences China University of Petroleum Beijing College of Environmental Science and Engineering Nankai University

A temperature-controlled ionic liquid dispersive liquid-phase microextraction in combination with high performance liquid chromatography was developed for the enrichment and determination of triazine herbicides such as cyanazine,simazine,and atrazine in water samples.1-Octyl-3-methylimidazolium hexafluorophosphate（[C8MIM][PF6]） was selected as the extraction *** experimental parameters were *** the optimal conditions,the linear range for cyanazine was in the concentration range of 0.5–80 mg/L and the linear range for simazine and atrazine was in the range of1.0–100 mg/*** limit of detection（LOD,S/N = 3） was in the ranges of 0.05–0.06 mg/L,and the intra day and inter day precision（RSDs,n = 6） was in the ranges of 3.2%–6.6% and 4.8%–8.9%,*** real water samples were analyzed with the developed method,and the experimental results showed that the spiked recoveries were *** these exhibited that the developed method was a valuable tool for monitoring such pollutants.

关键词： Temperature-controlled ionic liquid dispersive liquid-phase microextraction Ionic liquid Triazine herbicides High performance liquid chromatography

Velocity Calculation by Automatic Camera Calibration Based on Homogenous Fog Weather Condition

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International Journal of Automation and computing 2013年第2期10卷 143-156页

作者： Hong-Jun Song Yang-Zhou Chen yuan-yuan gao Center for Autonomous Technology and Intelligent Control Beijing University of Technology College of Information and Engineering Zhejiang Agriculture and Forestry University

A novel algorithm for vehicle average velocity detection through automatic and dynamic camera calibration based on dark channel in homogenous fog weather condition is presented in this paper. Camera fixed in the middle of the road should be calibrated in homogenous fog weather condition, and can be used in any weather condition. Unlike other researches in velocity calculation area, our traffic model only includes road plane and vehicles in motion. Painted lines in scene image are neglected because sometimes there are no traffic lanes, especially in un-structured traffic scene. Once calibrated, scene distance will be got and can be used to calculate vehicles average velocity. Three major steps are included in our algorithm. Firstly, current video frame is recognized to discriminate current weather condition based on area search method （ASM）. If it is homogenous fog, average pixel value from top to bottom in the selected area will change in the form of edge spread function （ESF）. Secondly, traffic road surface plane will be found by generating activity map created by calculating the expected value of the absolute intensity difference between two adjacent frames. Finally, scene transmission image is got by dark channel prior theory, camera s intrinsic and extrinsic parameters are calculated based on the parameter calibration formula deduced from monocular model and scene transmission image. In this step, several key points with particular transmission value for generating necessary calculation equations on road surface are selected to calibrate the camera. Vehicles pixel coordinates are transformed to camera coordinates. Distance between vehicles and the camera will be calculated, and then average velocity for each vehicle is got. At the end of this paper, calibration results and vehicles velocity data for nine vehicles in different weather conditions are given. Comparison with other algorithms verifies the effectiveness of our algorithm.

关键词： Vehicle velocity calculation homogenous fog weather condition dark channel prior monocular camera calibration

Microbial Transformation of Pregnane-3β,16β,20-triol by Cunninghamella echinulata

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Chinese Journal of Chemistry 2013年第1期31卷 127-131页

作者： Chenru Yang Huafang Fan yuan yuan Jinming gao Shaanxi Engineering Center of Bioresource Chemistry & Sustainable Utilization College of Science Northwest Adow University Yangling Shaanxi 712100 China

Microbial transformation of pregnane-3β,16β,20-triol （1） by Cunninghamella echinulata afforded four previ- ously unreported polyhydroxylated steroids, namely pregnane-3β,14a,16β,20-tetrol （2）, pregnane-3-oxo-14a, 16β,20-triol （3）, pregnane-3-oxo-7β,16β,20-triol （4）, and pregnane-3fl,7β,16β,20-tetrol （5）. The structures of these metabolites were established by means of spectroscopic analysis （1D and 2D NMR as well as HRESIMS analysis）.

关键词： pregnane-3β 16β,20-triol Cunninghamella echinulata biotransformation hydroxylation biocatalysis

Effect of arsenic trioxide on human hepatoma cell line BEL-7402 cultured in vitro

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World Journal of Gastroenterology 2000年第5期6卷 681-687页

作者： Hong Yu Xu You Lin Yang yuan yuan gao Qiao Li Wu Guang Qiang gao Department of Digestive Disease the First Hospital of Harbin Medical UniversityHarbin 150001Heilongjiang ProvinceChina Neurologic Cytology Unit The First Hospital of Harbin Medical UniversityHarbin 150001Heilongjiang ProvinceChina Department of Medical Laboratory Science The First Hospital of Harbin Medical UniversityHarbin 150001Heilongjiang ProvinceChina

AIM To study the effect of a varyingconcentrations of arsenic trioxide on humanhepatoma cell line BEL-?402 cultured in vitro andits mechanism of *** The BEL-7402 cells were treatedwith arsenic trioxide（at the concentrations of0.5,1,2 μmol/L,respectively）for 4 *** cell growth and proliferation wereobserved by cell counting and cell-growth *** changes were studied *** cytometry was usedto assay celI-DNA distribution and the proteinexpression of Bcl-2 and Bax detected byimmunocytochemical *** The cell growth was significantlyinhibited by varying concentrations of arsenictrioxide as revealed by cell counting and cell-growth curve,which was dose- and *** trioxide treatment at 0.5,1and 2 μmol/L resulted in a sub-G1 cell peak,theapoptosis rate of the control group was 9.31%and that of 0.5 μmol/L arsenic trioxide 15.53%,no significant difference was seen between *** apoptosis rates of 1,2 μmol/L arsenictrioxide were 19.10% and 21.87% respectively,which were much higher（both P0/G1 phase cells and increase of Sphase cells were observed by flow cytometry,suggesting the inhibition effect of 0.5,1,2 μmol/L arsenic trioxide on BEL-7402 cell lay in the G0/G1 *** changes such asintact cell membrane,nucleic condensation,apoptotic body formation were seen undertransmission electronmicrescopy,whereas the0.5 mol/L arsenic trioxide-treated BEL-7402cells showed decrease of nucleocytoplasmicratio,round nucleus,well-differentiatedorganelles in the *** processes andmicrovilli on the cell surface of the experimentalgroups under scanning electron microscopy weresignificantly *** expressions ofBcl-2 and Bax were detected in 1 and 2 μmol/Larsenic trioxide-treated cells,these were 46%,87.33% and 83.08%,95.83% respectively,among which that of Bax was more *** trioxide treatment at

关键词： arsenic trioxide hepatoma flow cytometry immunohistochemistry microscopy electron apoptosis gene expression

Effects of Lycium barbarum polysaccharide on the photoinduced autophagy of retinal pigment epithelium cells

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International Journal of Ophthalmology(English edition) 2022年第1期15卷 23-30页

作者： yuan-yuan gao Juan Li Jie Huang Wu-Jun Li Yang Yu Ningxia Medical University Yinchuan 750004Ningxia Hui Autonomous RegionChina Yulin Hospital of Traditional Chinese Medicine Yulin 719000Shaanxi ProvinceChina

AIM:To investigate the relationship between autophagy and apoptosis in photoinduced injuries in retinal pigment epithelium(RPE)cells and how Lycium barbarum polysaccharide(LBP)contributes to the increased of RPE cells to photoinduced ***:In vitro cultures of human RPE strains(ARPE-19)were prepared and randomly divided into the blank control,model,low-dose LBP,middle-dose LBP,high-dose LBP,and 3-methyladenine(3MA)*** viability of the RPE cells and apoptosis levels in each group were tested through cell counting kit-8(CCK8)method with a flow cytometer(Annexin V/PI double staining technique).The expression levels of LC3II,LC3I,and P62 proteins were detected with the immunofluorescence *** expression levels of beclin1,LC3,P62,PI3K,P-mTOR,mTOR,P-Akt,and Akt proteins were tested through Western ***:LBP considerably strengthens cell viability and inhibits the apoptosis of RPE cells after *** PI3K/Akt/mTOR signal pathway is activated because of the upregulation of the phosphorylation levels of Akt and mTOR proteins,and thus autophagy is ***:LBP can inhibit the excessive autophagy in RPE cells by activating the PI3K/Akt/mTOR signaling pathways and thereby protect RPE cells from photoinduced injuries.

关键词： autophagy Lycium barbarum polysaccharide retinal pigment epithelial cell photoinduced apoptosis

A thermosensitive chitosan-based hydrogel for controlled release of insulin

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Frontiers of Materials Science 2014年第2期8卷 142-149页

作者： Ting-Ting gao Ming KONG Xiao-Jie CHENG Gui-Xue XIA yuan-yuan gao Xi-Guang CHEN Dong Su CHA HyunJin PARK ColLege of Marine Life Science Ocean University of China Qingdao 266003 China College of Life Sciences and Biotechnology Korea University Seoul 136-701 Korea

Present study aims at synthesizing a thermosensitive hydrogel for controlled release of insulin. According to a modified method, hydroxybutyl chitosan （HBC） hydrogel possessed thermal sensitivity is prepared which can form hydrogel at over 25℃. The HBC hydrogel is non-cytotoxic to mice fibroblasts cells （L929）. Insulin is 100% entrapped in the hydrogel, 38% of which is released in vitro from the concentration of 5% hydrogel after 48 h, whereas by enzymolysis with lysozyme, 80% of the total insulin is released after 48h. This study suggests that HBC hydrogel could be utilized for controlled release of insulin in a non-invasive manner.

关键词： hydroxybutyl chitosan hydrogel thermosensitive insulin controlledrelease

Effectiveness of groundwater extraction in Beijing since the ingauration of the first phase of the South-to-North Water Diversion Project, China

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Journal of Groundwater Science and Engineering 2023年第3期11卷 294-307页

作者： yuan-yuan gao Qing-yu Sun Ai-xin Wen Yan-pei Cheng Bureau of South to North Water Transfer of Planning Designing and ManagementMinistry of Water ResourcesBeijing 100038China Institute of Hydrogeology and Environmental Geology Chinese Academy of Geological SciencesShijiazhuang 050061China

This study assess the effectiveness of groundwater pressure extraction in Beijing since the opening of the first phase of the South-to-North Water Diversion Project,using survey and evaluation ***,an analysis of water consumption structure and the usage of diverted river water in Beijing in recent years was ***,the volume of groundwater pressure extraction in Beijing after the 33 project's inauguration was examined,revealing a decrease from 1.96 billion m^(3) in 2014 to 1.35 billion m^(3) in *** proportion of water supply reduced from 52.3%in 2014 to 33.3%in 2020,leading to an optimized water supply *** the end of 2020,groundwater pressure extraction in Beijing is estimated at 446 million m3,with a substantial reduction in over-exploitation of *** resources have been effectively replenished,and the strategic reserve capacity has been ***,this study evaluates the change in groundwater levels as an indicator of the effectiveness of pressure *** declining trend of groundwater levels in Beijing has been effectively mitigated,and there has been a consistent rebound in groundwater levels over the past five years.

关键词： Beijing City South-to-North Water Diversion Groundwater Over-exploitation Groundwater Pressure Extraction

The role of pineal microRNA-325 in regulating circadian rhythms after neonatal hypoxic-ischemic brain damage

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Neural Regeneration Research 2021年第10期16卷 2071-2077页

作者： Ning Sha Hua-Wei Wang Bin Sun Min Gong Po Miao Xiao-Lu Jiang Xiao-Feng Yang Mei Li Li-Xiao Xu Chen-Xi Feng yuan-yuan Yang Jie Zhang Wen-Jing Zhu yuan-yuan gao Xing Feng Xin Ding Soochow Key Laboratory of Prevention and Treatment of Child Brain injury Children’s Hospital of Soochow UniversitySuzhouJiangsu ProvinceChina Department of Pediatrics Affiliated Huai’an Hospital of Xuzhou Medical UniversityHuai’anJiangsu ProvinceChina Pediatrics Research Institute Children’s Hospital of Soochow UniversitySuzhouJiangsu ProvinceChina Department of Pediatrics The First Affiliated Hospital of Soochow University(Dushuhu Branch)SuzhouJiangsu ProvinceChina Cambridge-SU Genomic Resource Center Soochow UniversitySuzhouJiangsu ProvinceChina Clinical Laboratory Children’s Hospital of Soochow UniversitySuzhouJiangsu ProvinceChina

Circadian rhythm disorder is a common,but often neglected,consequence of neonatal hypoxic-ischemic brain damage(HIBD).However,the underlying molecular mechanisms remain largely *** previously showed that,in a rat model of HIBD,up-regulation of microRNA-325(miR-325)in the pineal gland is responsible for the suppression of Aanat,a key enzyme involved in melatonin synthesis and circadian rhythm *** better understand the mechanism by which miR-325 affects circadian rhythms in neonates with HIBD,we compared clinical samples from neonates with HIBD and samples from healthy neonates recruited from the First Affiliated Hospital of Soochow University(Dushuhu Branch)in *** found that circulating miR-325 levels correlated positively with the severity of sleep and circadian rhythm disorders in neonates with ***,a luciferase reporter gene assay revealed that LIM homeobox 3(LHX3)is a novel downstream target of *** addition,in miR-325 knock-down mice,the transcription factor LHX3 exhibited an miR-325-dependent circadian pattern of expression in the pineal *** established a neonatal mouse model of HIBD by performing doublelayer ligation of the left common carotid artery and exposing the pups to a low-oxygen environment for 2 ***3 mRNA expression was significantly down-regulated in these mice and partially rescued in miR-325 knockout mice subjected to the same ***,we showed that improvement in circadian rhythm-related behaviors in animals with HIBD was dependent on both miR-325 and *** together,our findings suggest that the miR-325-LHX3 axis is responsible for regulating circadian rhythms and provide novel insights into the identification of potential therapeutic targets for circadian rhythm disorders in patients with neonatal *** clinical trial was approved by Institutional Review Board of Children’s Hospital of Soochow University(approval No.2015028)on July 20,*** experiments were approved by An

关键词： brain injury circadian rhythm hypoxic-ischemic brain damage miRNA neonate pineal gland sleep transcription factor

Post-synthetic modifications of MOFs by different bolt ligands for controllable release of cargoes

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Chinese Chemical Letters 2024年第10期35卷 404-407页

作者： Hao Wang Meng-Qi Pan Ya-Fei Wang Chao Chen Jian Xu yuan-yuan gao Chuan-Song Qi Wei Li Xian-He Bu College of New Materials and Chemical Engineering Beijing Key Lab of Special Elastomeric Composite MaterialsBeijing Institute of Petrochemical TechnologyBeijing 102617China School of Materials Science and Engineering National Institute for Advanced MaterialsTKL of Metal and Molecule-Based Material ChemistryNankai UniversityTianjin300350China College of Chemical Engineering Inner Mongolia University of TechnologyHohhot 010051China

Post-synthetic modifications(PSM)have drawn great attention as a vigoroso tool to tune or enhance the performance of metal-organic frameworks(MOFs).However,the current PSM method usually have to sacrifice the porosity of MOFs to enrich their functionality,such as pore space partition(PSP)and postsynthetic elimination and insertion(PSE&I),causing a trade-off in this *** address this issue,we herein propose a new PSM strategy of using the size-matching ligands as the bolts to lock MOFs'pores,which could be anchored onto open metal sites(OMSs)after guest loading through a stepwise *** a result,the loaded cargoes undergo a controlled releasing process with respect to different bolt *** proposed strategy provides a promising way to balance the functionality and porosity of MOFs.

关键词： Post-synthetic modifications Metal-organic frameworks Bolting ligand Control release Cargo

Apoptosis,proliferation and p53 gene expression of *** associated gastric epithelial lesions

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World Journal of Gastroenterology 2001年第6期7卷 779-782页

作者： Zhong Zhang~1 yuan yuan Hua gao Ming Dong Lan Wang Yue-Hua Gong 1 Department of Pathology,Shenyang Medical College,Shenyang 110031 Liaoning Province,China Department of Pathology Shenyang Medical College Liaoning Province China.

AIM: To study the relationship between Helicobacter pylori (H. pylori) and gastric carcinoma and its possible pathogenesis by H. pylori. METHODS: DNEL technique and immunohistochemical technique were used to study the state of apoptosis, proliferation and p53 gene expression. A total of 100 gastric mucosal biopsy specimens, including 20 normal mucosa, 30 H. pylori-negative and 30 H. pylori-positive gastric precancerous lesions along with 20 gastric carcinomas were studied. RESULTS: There were several apoptotic cells in the superficial epithelium and a few proliferative cells within the neck of gastric glands, and no p53 protein expression in normal mucosa. In gastric carcinoma, there were few apoptotic cells, while there were a large number of proliferative cells, and expression of p53 protein significantly was increased. In the phase of metaplasia, the apoptotic index (AI, 4.36%+/-1.95%), proliferative index (PI, 19.11%+/-6.79%) and positivity of p53 expression (46.7%) in H. pylori-positive group were higher than those in normal mucosa (P<0.01). AI in H. pylori-positive group was higher than that in H. pylori-negative group (3.81%+/-1.76%), PI in H. pylori-positive group was higher than that in H. pylori-negative group (12.25%+/-5.63%, P<0.01). In the phase of dysplasia, AI (2.31%+/-1.10%) in H. pylori-positive group was lower (3.05%+/-1.29%) than that in H. pylori-negative group, but PI (33.89%+/-11.65%) was significantly higher (22.09+/-8018%, P<0.01). In phases of metaplasia, dysplasia and gastric cancer in the H. pylori-positive group, AIs had an evidently graduall decreasing trend (P<0.01), while PIs had an evidently gradual increasing trend (P<0.05 or P<0.01), and there was also a trend of gradual increase in the expression of p53 gene. CONCLUSION: In the course of the formation of gastric carcinoma, proliferation of gastric mucosa can be greatly increased by H. pylori, and H. pylori can induce apoptosis in the phase of metaplasia, but in th

关键词： Apoptosis Gene Expression Helicobacter pylori Cell Division Gastric Mucosa Genes, p53 Helicobacter Infections Humans Research Support, Non-U.S. Gov't Stomach Diseases