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Signal Transduction and Targeted Therapy 2022年第2期7卷 335-336页

作者： Yier Lu Chenyang Ye ying yuan Department of Medical Oncology the Second Affiliated Hospital of Zhejiang University School of MedicineHangzhouZhejiang 310009China Cancer Institute(Key Laboratory of Cancer Prevention and Intervention China National Ministry of Education)the Second Affiliated HospitalSchool of MedicineZhejiang UniversityHangzhouZhejiang 310009China

The diversity of CD8^(+)tumor-infiltrating lymphocyte(TIL)phenotypes has been described in several cancer types,but the specific relevance remains *** a recent article published in Nature,Oliveira et al.1 unveiled the relationship between phenotypic properties and specificities of TCRs and they highlighted that the exhausted states are enriched in cells with antitumor reactivity.

关键词： melanoma infiltrating

Effect of Chinese herbal compound on liver fibrosis in rabbits with schistosomiasis by B-ultrasound

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Asian Pacific Journal of Tropical Medicine 2013年第8期6卷 658-662页

作者： Xiao-Lu Liang Jia-ying yuan Department of Ultrasonography Military General Hospital of Beijing PLA Department of Ultrasonography Directly Affiliated Hospital of Henan Military Area

Objective: To explore the value of B-ultrasound on the evaluation of the effects of traditional Chinese medicine compound of Radix astragali , Salvia miltiorrhiza and Angelica sinensis , and TCM + praziquantel on liver fibrosis in rabbits with schistosomiasis. Methods: The hepatic fibrosis model in rabbits with schistosomiasis was established. The experimental animals (24 rabbits) were randomly divided into four groups (group A, B, C and D, n =6). Group A (control group) was only treated by praziquantel; Group B was treated by mixture of Radix astragali and Salvia miltiorrhiza + praziquantel; Group C was treated by mixture of Radix astragali and Angelica sinensis + praziquantel; Group D was treated by mixture of Radix astragali, Salvia miltiorrhiza and Angelica sinensis + praziquantel. Then B-ultrasonogram was used to evaluate the effects. Results: Each group showed certain curative effect on liver fibrosis in rabbits with schistosomiasis. The efficacy of group B, C and D was better than group A, and that of group D was the best. The differences in long diameter, thickness diameter, transverse diameter and portal vein inner diameter of liver before and after treatment were statistically significant ( P <0.05). The liver function indexes and liver fibrosis indexes were significantly improved after treatment ( P <0.05). Conclusions: The mixture of Radix astragali, Salvia miltiorrhiza and Angelica sinensis combined with Western medicine treatment can obviously improve the efficacy on liver fibrosis of schistosomiasis.

关键词： Schistosomiasis Liver fibrosis Chinese herbal compound Praziquantel B-ultrasound

Insignin A, A Novel C_21-Steroidal Aglycone from Biondia insignis

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Chinese Chemical Letters 2000年第12期11卷 1065-1068页

作者： yuan Hu ZHANG Yue Mao SHEN yuan ying WEN Ting Yun KUANG Laboratory of Phytochemistry and Department of Plant Biotechnology Kunming Institute of BotanyChinese Academy of SciencesKunming 650204 Laboratory of Phytochemistry Institute of BotanyChinese Academy of SciencesBeijing 100093

Insignin A, a new C21-steroidal aglycone having the rare 15,16-seco pregnane skeleton was isolated from the acidic hydrolysis part of the 95% EtOH extract of Biondia insignis. It's structure was identified to be 15R,... 详细信息

关键词： Asclepiadaceae Biondia insignis C21-steroidal aglycone insignin A<

基于网络药理学和动物实验研究麝香通心滴丸防治糖尿病心肌病的作用机制

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中国中药杂志 2024年第7期49卷 1905-1914页

作者：白亚利崔鑫钰袁悦莹张雨婷王伟刘天华吴晏北京中医药大学中医学院北京100029 北京中医药大学证候与方剂基础研究教育部重点实验室北京100029 证候与方剂基础研究北京市重点实验室北京100029 北京中医药大学中医药研究院北京100029

通过网络药理学、分子对接及动物实验探究麝香通心滴丸(Shexiang Tongxin Dropping Pills,STDP)防治糖尿病心肌病(diabetic cardiomyopathy,DCM)的作用机制。借助BATMAN、TCMSP、GeneCards等数据库筛选STDP防治DCM的活性成分及作用靶点,利用STRING数据库及Cytoscape软件构建STDP防治DCM作用靶点的蛋白-蛋白相互作用(protein-protein inte-raction,PPI)网络及“药物-活性成分-靶点”网络,通过DAVID数据库对作用靶点进行GO和KEGG富集分析,采用AutoDock Vina软件对关键信号通路核心受体蛋白与其对应活性成分进行分子对接验证。采用高脂喂养联合腹腔注射链脲佐菌素的方法建立DCM大鼠模型,分为对照组、模型组、STDP低剂量组(20 mg·kg^(-1))、STDP高剂量组(40 mg·kg^(-1))、二甲双胍组(200 mg·kg^(-1));连续给药8周后超声心动图检测各组大鼠心功能,苏木精-伊红(hematoxylin-eosin,HE)染色观察各组大鼠心肌病理改变,天狼猩红染色检测各组大鼠心肌胶原纤维沉积,WGA染色检测心肌肥大程度,蛋白免疫印迹法检测各组大鼠心肌组织中p38丝裂原活化蛋白激酶(p38 mitogen-activated protein kinase,p38)、磷酸化p38(phosphorylation-p38,p-p38)、c-Jun氨基末端激酶(c-Jun N-terminal kinase,JNK)、磷酸化JNK(phosphorylation-JNK,p-JNK)、caspase-3及C-caspase-3蛋白的表达。网络药理学筛选得到STDP活性成分199种,对应靶点1655个,与463个DCM疾病靶点取交集,得到134个STDP防治DCM的潜在作用靶点,并筛选出糖尿病并发症中的AGE-RAGE信号通路;分子对接结果显示miltirone、dehydromiltirone和tryptanthrin与RAGE均有较好的结合力。动物实验结果证实,STDP可有效保护DCM大鼠心功能,与模型组比较,STDP低、高剂量组p-p38、p-JNK、C-caspase-3蛋白表达水平均显著降低。综上,SDTP可能通过影响AGE-RAGE信号通路发挥对DCM大鼠心功能的保护作用。

关键词：麝香通心滴丸糖尿病心肌病网络药理学分子对接动物实验 AGE-RAGE信号通路心功能

Influence of Nucleotide-biased Fluorescence Emissions of SYBR Green Ⅱ on the Result Consistence of Rolling Circle Amplification

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Chemical Research in Chinese Universities 2019年第6期35卷 1119-1123页

作者： ZHANG Bin JIANG Jiquan yuan ying GUAN Yifu Department of Biochemistry and Molecular Biology China Medical UniversityShenyang 110122P.R.China Department of Plastic Surgery School of StomatologyChina Medical UniversityShenyang 110009P.R.China Department of Biochemistry and Molecular Biology College of Medical Laboratory and TechnologyHarbin Medical University(Daqing)Daqing 163000P.R.China

The fluorescence dye SYBR Green Ⅱ(SG Ⅱ)has been frequently used in rolling circle amplification(RCA)based analyses of nucleic ***,a good amount of inconsistencies have been reported in regards the quality and reproducibility of RCA *** properly examine this experimental issue,here we utilized a series of synthetic oligonucleotides and circular templates to investigate the impact of SG Ⅱ in RCA *** results indicate that SG Ⅱ enables a strong fluorescence signal only when complexing with guanosine(G)*** RCA reactions,long single-stranded RCA products,enriched with G residues,result in higher fluorescence emission when compared with the addition of other nucleotide *** results suggest that the nucleotide composition of the reaction can affect the amplification results and,eventually,can lead to inconsistent fluorescence of the RCA *** work indicates that particular attention should be given when circular templates are designed for the quantitative analysis of nucleic acids,to further allow the signal reproducibility of RCA-based experiments.

关键词： Rolling circle amplification SYBR Green Ⅱ Nucleotide-biased Fluorescence emission

乳腺超声造影预测HER-2阳性乳腺癌曲妥珠单抗化疗敏感性的价值

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海南医学 2024年第18期35卷 2676-2679页

作者：袁莹莹蔡艳周晓霞郑国连湛江中心人民医院超声医学科广东湛江524000

目的研究乳腺超声造影预测人类表皮生长因子受体2(HER-2)阳性乳腺癌患者对曲妥珠单抗化疗敏感性的价值。方法回顾性分析2023月1月至2024年1月湛江中心人民医院诊疗的187例HER-2阳性乳腺癌患者的临床和影像资料,所有患者均接受曲妥珠单... 详细信息

目的研究乳腺超声造影预测人类表皮生长因子受体2(HER-2)阳性乳腺癌患者对曲妥珠单抗化疗敏感性的价值。方法回顾性分析2023月1月至2024年1月湛江中心人民医院诊疗的187例HER-2阳性乳腺癌患者的临床和影像资料,所有患者均接受曲妥珠单抗治疗,按照治疗第1个周期(3周)的疗效分为不敏感组122例和敏感组65例,所有患者在治疗第1个周期结束后均接受乳腺超声造影检查,比较两组患者治疗第1个周期结束后的乳腺超声造影表现。结果治疗敏感组患者病灶的最大径为(2.91±0.33)cm,明显小于不敏感组患者的(4.67±0.75)cm,差异有统计学意义(P<0.05);治疗不敏感组患者的高增强率、快进增强模式、增强后肿物不清晰、增强后肿块形态不规则、周围有穿行血管的占比分别为100.00%、100.00%、79.51%、86.07%、100.00%,明显高于治疗敏感组患者的83.08%、60.00%、63.08%、69.23%、80.00%,差异均有统计学意义(P<0.05)。结论乳腺超声造影在预测HER-2阳性乳腺癌曲妥珠单抗治疗敏感性中有一定价值,可指导临床治疗。

关键词：乳腺癌人类表皮生长因子受体2阳性曲妥珠单抗乳腺超声造影化疗敏感性

胎儿脊髓圆锥末端尾侧椎体骨化中心自动计数模型研究

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中华超声影像学杂志 2024年第8期33卷 677-682页

作者：郭志伟文华轩罗丹丹梁博诚谭光华张宏杰谭莹袁鹰李胜利南方医科大学第一临床医学院广州510515 深圳市妇幼保健院超声科深圳518028 珠海市人民医院(暨南大学珠海临床医学院)超声影像科珠海519000 湖南大学信息科学与工程学院长沙410082

目的研发胎儿脊髓圆锥末端尾侧椎体骨化中心智能计数模型并评估其应用效果。方法回顾性选取2021年1月至2022年10月深圳市妇幼保健院及珠海市人民医院采集的3000帧胎儿脊柱骶尾段正中矢状面声像图,其中2800张图像进行椎体骨化中心与脊髓... 详细信息

目的研发胎儿脊髓圆锥末端尾侧椎体骨化中心智能计数模型并评估其应用效果。方法回顾性选取2021年1月至2022年10月深圳市妇幼保健院及珠海市人民医院采集的3000帧胎儿脊柱骶尾段正中矢状面声像图,其中2800张图像进行椎体骨化中心与脊髓圆锥的人工精细标注与训练,采用Yolov8算法搭建实例分割模型进行分割训练并通过后处理的方式进行椎体骨化中心拟合与自动计数。另外200张图像分别由人工智能(AI)模型、中级医师(D1)以及初级医师(D2)对胎儿脊髓圆锥末端尾侧椎体骨化中心个数进行计数并计时,由专家对AI模型和D1、D2的计数是否准确进行评估。比较AI模型、D1和D2的准确率以及耗时差异。结果经过专家评估,AI圆锥分割拟合与计数的准确率达95.00%(190/200),与D1的准确率(94.50%,189/200)差异无统计学意义(P=0.823),但高于D288.50%(177/200),差异有统计学意义(P=0.012)。D1、D2与AI计数耗时分别为5.00(4.25,6.00)s、7.00(7.00,8.00)s、0.09(0.08,0.10)s,AI明显少于D1、D2,差异有统计学意义(均P<0.001)。结论经过训练的AI模型可以高效、准确地完成圆锥末端的椎体骨化中心计数,相当于中级医师的水平,该模型有望进一步应用于胎儿脊柱裂筛查,提高产前超声筛查的自动化与智能化水平。

关键词：超声筛查人工智能胎儿脊髓圆锥脊柱裂

PCSK9抑制药的药理作用机制研究现状及临床应用探讨

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中国临床药理学杂志 2024年第16期40卷 2438-2441页

作者：莫文慧徐思蕾何霞白妞妞袁梦莹李志敏张娇王菲郑湲坤四川省医学科学院·四川省人民医院药学部四川成都610072 电子科技大学医学院个体化药物治疗四川省重点实验室四川成都610054 山西医科大学药学院山西太原030001 重庆市红十字会医院(江北区人民医院)药学部重庆400020

由脂质代谢紊乱引起的动脉粥样硬化是动脉粥样硬化性心血管疾病的主要病理基础。他汀类药物是这类疾病调脂治疗的基石,但在实际应用中部分患者仍存在血脂管理不达标的状况。前蛋白转化酶枯草溶菌素9(PCSK9)抑制药作为一类新型调脂药物... 详细信息

由脂质代谢紊乱引起的动脉粥样硬化是动脉粥样硬化性心血管疾病的主要病理基础。他汀类药物是这类疾病调脂治疗的基石,但在实际应用中部分患者仍存在血脂管理不达标的状况。前蛋白转化酶枯草溶菌素9(PCSK9)抑制药作为一类新型调脂药物被逐渐应用于该类患者治疗,近年来的研究表明PCSK9抑制药除了能调节脂质代谢,还具有潜在的抗炎、抗血小板活化等作用。本文梳理了PCSK9抑制药多个药理作用机制及临床研究现状,探讨可能影响PCSK9抑制药疗效的因素,以期为PCSK9抑制药的安全合理用药提供参考。

关键词：前蛋白转化酶枯草溶菌素9抑制药动脉粥样硬化性心血管疾病作用机制应用现状

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基于CT影像组学预测肺癌椎体转移的研究

中国CT和MRI杂志 2024年第7期22卷 166-168页

作者：李倩张堃王曼袁佳莹湖南省脑科医院放射科湖南中医药大学第一附属医院放射科湖南长沙410007

目的探讨基于CT平扫图像的影像组学方法在预测肺癌椎体早期转移中的应用价值。方法回顾性分析2016年1月至2019年7月间于我院就诊的肺癌椎体转移病例。按照2:1将样本随机分为训练集(n=80)和测试集(n=40)。采用3D-SLICER软件进行图像分割... 详细信息

目的探讨基于CT平扫图像的影像组学方法在预测肺癌椎体早期转移中的应用价值。方法回顾性分析2016年1月至2019年7月间于我院就诊的肺癌椎体转移病例。按照2:1将样本随机分为训练集(n=80)和测试集(n=40)。采用3D-SLICER软件进行图像分割与特征提取,使用绝对最小收缩和选择算子(LASSO)回归对影像组学特征进行降维,采用多因素回归分析潜在的独立临床预测因子,分别建立影像组学回归模型及临床-影像组学联合模型,并构建临床-影像组学列线图。结果影像组学预测模型、临床-影像组学联合模在训练集的AUC值分别为0.786(0.685-0.887)、0.819(0.728-0.911),在测试集的AUC值分别为0.750(0.589-0.911)、0.831(0.669-0.992)。结论基于CT平扫图像的影像组学方法可有效预测肺癌椎体早期转移的风险,构建临床-影像组学列线图可作为辅助诊断肺癌椎体早期转移的可视化工具,为肺癌骨转移的临床决策提供支持。

关键词：影像组学计算机断层扫描肺癌脊柱转移瘤

Removal of refractory organics and heavy metals in landfill leachate concentrate by peroxi-coagulation process

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Journal of Environmental Sciences 2022年第6期34卷 43-51页

作者： Dun Liu ying yuan Yuquan Wei Hao Zhang Yanxiao Si Fang Zhang School of Environment and State Key Joint Laboratory of Environment Simulation and Pollution Control Tsinghua UniversityBeijing 100084China Beijing Advanced Innovation Center for Big Data-based Precision Medicine Beihang UniversityBeijing 100191China

Landfill leachate is a complex effluent and it is difficult to deal with. Electrochemical methods have been considered as a promising alternative technology for treatment of landfill leachate with refractory organic contaminants and heavy metals. Peroxi-coagulation(PC)process with iron anode and modified graphite felt cathode was developed for efficient landfill leachate concentrate treatment. Compared to electro-Fenton(EF) and electrocoagulation(EC) processes, the PC process was more cost-effective due to the combined action of·OH oxidation and iron hydroxides coagulation. A maximal TOC removal of 77.2% ± 1.4% was obtained after 360 min at initial p H = 5.0 and current density of 10 m A/cm^(2). After the PC process, concentrations of all seven heavy metals in the final effluents were below the allowable emission limits given by the present regulatory standards. The method preference for heavy metal removal was PC > EC > EF. Based on the three-dimensional fluorescence spectroscopy coupled with regional integration analysis during the PC treatment, the florescence peaks of both humic acids and fulvic acids disappeared after treatment for 360min. Decreasing trends were observed in the fluorescent regional standard volumes for aromatic protein Ⅰ(31.4%), aromatic protein Ⅱ(63.7%), fulvic acid-like(69.5%), soluble microbial by-product-like(75%) and humic acid-like regions(76.3%). The results indicate that comparing to the EF and EC process, the PC process provide a promising and more cost-effective alternative for the treatment of landfill leachate concentrate.

关键词： Landfill leachate Peroxi-coagulation Electro-Fenton Electrocoagulation Modified cathode