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Divergence of trafficking and polarization mechanisms for PIN auxin transporters during land plant evolution

Plant Communications 2024年第1期5卷 116-129页

作者： Han Tang Kuan-Ju Lu yuzhou zhang You-Liang Cheng Shih-Long Tu Jiri Friml Institute of Science and Technology Austria(ISTA) Am Campus 13400 KlosterneuburgAustria Graduate Institute of Biotechnology National Chung Hsing UniversityNo.145Xingda Rd.South Dist.Taichung 40227TaiwanR.O.C College of Life Sciences Northwest A&F UniversityShaanxiYanglingChina Institute of Plant and Microbial Biology Academia Sinica128 Sec.2Academia RdNankangTaipei 11529TaiwanR.O.C Present address:Graduate Institute of Biochemistry National Chung Hsing UniversityNo.145Xingda Rd.South Dist.Taichung 40227TaiwanR.O.C

The phytohormone auxin,and its directional transport through tissues,plays a fundamental role in the development of higher *** polar auxin transport predominantly relies on PIN-FORMED(PIN)auxin ***,PIN polarization is crucial for development,but its evolution during the rise of morpho-logical complexity in land plants remains ***,we performed a cross-species investigation by observing the trafficking and localization of endogenous and exogenous PINs in two bryophytes,Physco-mitrium patens and Marchantia polymorpha,and in theflowering plant Arabidopsis *** confirmed that the GFP fusion did not compromise the auxin export function of all examined PINs by using a radioac-tive auxin export assay and by observing the phenotypic changes in transgenic *** PINs polarize tofilamentous apices,while exogenous Arabidopsis PINs distribute symmetrically on the membrane in both *** the Arabidopsis root epidermis,bryophytic PINs have no defined *** interference revealed a strong cytoskeletal dependence of bryophytic but not Arabidopsis PIN *** divergence of PIN polarization and trafficking is also observed within the bryophyte clade and between tissues of individual *** results collectively reveal the divergence of PIN traf-ficking and polarity mechanisms throughout land plant evolution and the co-evolution of PIN sequence-based and cell-based polarity mechanisms.

关键词： Arabidopsis evolution Marchantia Physcomitrium PINs polarization protein trafficking

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Beneficial and Biocontrol Effects of Trichoderma atroviride,a Dominant Species in White Birch Rhizosphere Soil

Beneficial and Biocontrol Effects of Trichoderma atroviride,...

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中国植物病理学会2024年学术年会

作者： LIU Kuo zhang yuzhou DU Huaying WANG Zhiying GU Peiwen LIU Zhihua YU Zeyang School of Agriculture Ningxia University Ningxia Forest Disease and Pest Control and Quarantine Station School of Forestry Northeast Forestry University College of Forestry Shenyang Agricultural University

White birch(Betula platyphylla Suk.) is a typical pioneer tree species that is important in forest restoration in northern China,Japan,and *** the present study,37 isolates were obtained from *** rhizosphere soils in Heilongjiang Provincethey were identified as ***(3 isolates),***(2 isolates),***(8 isolates),***(21 isolates,dominant species) and ***(3 isolates).Stress tolerance tests(salt,alkali,and nutritional stress that simulated saline alkali or barren soil) and confrontation assays(with four pathogens) were performed to determine which isolates had good biocontrol ability in barren soilthe results show that *** was ***,in order to determine the effect of *** on plants and soil,Gynura cusimbua seeds were sown and treated with a *** spore suspension,as was unsown *** seedlings treated using *** had significantly greater height,stem diameter,soluble protein content,soluble sugar content,and malonaldehyde(MDA) content and their catalase(CAT) activity was also significantly *** addition,when the plants were inoculated with Alternaria alternata,the plants treated using *** had stronger CAT activity,significantly higher soluble protein content and soluble sugar content,and significantly lower MDA content,which indicates stronger resistance and less injury caused by the *** addition,*** not only increased the content of available nitrogen and available phosphorus in the soil,but also promoted *** seedlings' absorption of available nitrogen and available ***,the characteristics of *** may make it the main factor that helps *** colonise cut-over ***,a promising biocontrol candidate,can be used in agriculture and forestry.

关键词： Trichoderma identification resistance induction pioneer tree species growth promotion soil inprovement

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小麦根系构型及抗旱性研究进展

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中国农业科学 2024年第9期57卷 1633-1645页

作者：张余周王一钊高茹茜刘逸凡西北农林科技大学生命科学学院

小麦是全球最重要的粮食作物之一，干旱是影响其生长发育最重要的非生物胁迫因子。根系作为作物获取水分和养分的重要器官，直接决定了作物对土壤水分的利用效率。近年来，越来越多的研究表明，根系构型在植物干旱胁迫响应中发挥了重要... 详细信息

小麦是全球最重要的粮食作物之一，干旱是影响其生长发育最重要的非生物胁迫因子。根系作为作物获取水分和养分的重要器官，直接决定了作物对土壤水分的利用效率。近年来，越来越多的研究表明，根系构型在植物干旱胁迫响应中发挥了重要功能。本文综述了目前根系构型在调控小麦抗旱性方面的研究进展。首先概述了根向性生长，特别是根向重力性生长对植物根系结构的塑造作用，重点总结了目前挖掘到参与根系向重力性生长的相关基因及其分子调控机制，并阐述了根向性生长调控的根系构型是如何介导小麦对干旱胁迫的适应。除了根向性生长，根系的发育过程也参与了对植物根系构型的调控，并决定植物对干旱胁迫的适应能力，因此，本文进一步综述了在干旱胁迫条件下小麦如何通过调控根系发育来改变根系形态，包括增加根长、调控侧根数量和根毛密度等，来增强小麦对土壤水分的吸收和对干旱环境的适应；同时，系统总结了干旱胁迫条件下参与调控作物（尤其是小麦）根系发育的相关基因。此外，根系作为植物地下部分，其构型的解析一直是本领域研究难点，阻碍了对根系结构与植物耐旱性关系的进一步解析，因此，本文也归纳了目前可用于小麦根系二维结构和三维结构表型分析的技术。这些技术可测量和分析小麦根系的长度、密度、生长方向和形态等参数，为深入理解根系构型与小麦抗旱性关系提供技术支撑。最后，展望了改良根系结构在小麦抗旱育种中的应用前景，并对如何挖掘更多潜在的小麦根系构型调控基因，及解析相关基因的调控机理进行了讨论。综上所述，小麦根系结构与小麦抗旱性关系密切，随着测序和分析技术的不断发展，以及小麦根系结构调控机制研究的不断深入，为未来培育抗旱小麦新品种提供了新的手段和策略。

关键词：小麦根系结构根系向性生长根系发育抗旱性根系可视化

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蓝萼乙素通过Akt/BAD通路对宫颈癌裸鼠移植瘤生长的影响

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现代药物与临床 2024年第6期39卷 1384-1389页

作者：张玉洲李晓敏孙少霖开封市妇产医院妇产科河南开封475000 开封市妇产医院病理科河南开封475000

目的探究蓝萼乙素通过蛋白激酶B(Akt)/B细胞淋巴瘤-2(Bcl-2)相关死亡启动因子(BAD)通路对宫颈癌裸鼠移植瘤生长的影响和机制。方法将48只SPF级雌性Balb/c-nu裸鼠随机分为模型、蓝萼乙素(56、112 mg/kg)和蓝萼乙素+SC79(112 mg/kg+10 m... 详细信息

目的探究蓝萼乙素通过蛋白激酶B(Akt)/B细胞淋巴瘤-2(Bcl-2)相关死亡启动因子(BAD)通路对宫颈癌裸鼠移植瘤生长的影响和机制。方法将48只SPF级雌性Balb/c-nu裸鼠随机分为模型、蓝萼乙素(56、112 mg/kg)和蓝萼乙素+SC79(112 mg/kg+10 mg/kg)组,每组12只。将HeLa细胞种植在裸鼠右大腿根部背侧皮下,建立皮下移植瘤模型,观察肿瘤体积、质量和一般情况的变化。苏木精–伊红(HE)染色观察肿瘤组织的组织学变化。TUNEL实验检测肿瘤组织细胞凋亡情况。Western blotting法检测移植瘤组织中p-Akt、Akt、p-BAD、BAD、磷酸酯酶与张力蛋白同源物(PTEN)蛋白表达水平。结果与模型组相比,蓝萼乙素组第22、25、28天裸鼠肿瘤体积、肿瘤质量、肿瘤组织Akt、BAD磷酸化水平显著降低(P<0.05),肿瘤细胞凋亡率、肿瘤组织PTEN蛋白水平显著升高(P<0.05);SC79可减弱蓝萼乙素对宫颈癌裸鼠移植瘤生长的抑制作用。结论蓝萼乙素对宫颈癌裸鼠移植瘤生长起到抑制作用,其作用机制与抑制Akt/BAD信号通路有关。

关键词：蓝萼乙素宫颈癌移植瘤蛋白激酶B B细胞淋巴瘤-2 磷酸酯酶与张力蛋白同源物

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The disease resistance potential of Trichoderma asperellum T-Pa2 isolated from Phellodendron amurense rhizosphere soil

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Journal of Forestry Research 2022年第1期33卷 321-331页

作者： Zeyang Yu Zhihua Liu yuzhou zhang Zhiying Wang School of Forestry Northeast Forestry University26 Hexing RoadHarbin 150040People’s Republic of China General Station of Forest and Grassland Pest Management National Forestry and Grassland AdministrationShenyang 110034People’s Republic of China College of Forestry Shenyang Agricultural UniversityShenyang 110866People’s Republic of China

To promote the application of Trichoderma,many countries have collected Trichoderma *** the present study,nine isolates were isolated from a rhizosphere soil of Phellodendron amurense and were identified as three species:Trichoderma brevicompactum(one isolate),***(two isolates),***(six isolates).Dual culture experiments showed that *** T-Pa2 grew fast and produced the best inhibition rates against six tested pathogens(80.25–91.65%)via competition and *** davidiana×*** *** Louche(PdPap poplar)was treated with T-Pa2,which increased the catalase activity,nitrate reductase activity,and content of osmosis molecules significantly(P<0.05).Meanwhile,induction by T-Pa2 increased the resistance of PdPap poplar against Alternaria alternata via modulating the expression of salicylic acid,jasmonic acid,and auxin transduction pathway *** results will form the basis for the collection and application of biocontrol agents in forestry.

关键词： Biological control Trichoderma identification Antifungal property Enzyme activity

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BIM技术在建筑绿色节能设计中的应用

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建设科技 2024年第4期 23-25页

作者：刘永利张豫洲青岛新锐家禾置业有限公司青岛市266000

BIM技术在建筑绿色节能设计中的应用不仅可以实现数字化目标,而且还可以减少工程的施工费用投入。基于此,本文结合BIM技术在建筑绿色节能设计中的应用特点,提出BIM技术在绿色建筑采光、室内外通风、日照分析等方面的应用措施,希望为提高... 详细信息

BIM技术在建筑绿色节能设计中的应用不仅可以实现数字化目标,而且还可以减少工程的施工费用投入。基于此,本文结合BIM技术在建筑绿色节能设计中的应用特点,提出BIM技术在绿色建筑采光、室内外通风、日照分析等方面的应用措施,希望为提高BIM技术在建筑绿色节能设计中的应用水平提供参考。

关键词： BIM技术绿色节能应用措施

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Recent advances in the research for the homolog of breast cancer associated gene AtROW1 in higher plants

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Science China(Life Sciences) 2016年第8期59卷 825-831页

作者： Yue Jiao yuzhou zhang Yu-Xian Zhu State Key Laboratory of Protein and Plant Gene Research College of Life Sciences Peking University Center for Science and Technology Development Ministry of Agriculture

BARD1(BRCA1 associated RING domain protein 1), as an important animal tumor suppressor gene associated with many kinds of cancers, has been intensively studied for decades. Surprisingly, homolog of BARD1 was found in plants and it was renamed At ROW1(repressor of Wuschel-1) according to its extremely important function with regard to plant stem cell homeostasis. Although great advances have been made in human BARD1, the function of this animal tumor-suppressor like gene in plant is not well studied and need to be further elucidated. Here, we review and summarize past and present work regarding this protein. Apart from its previously proposed role in DNA repair, recently it is found essential for shoot and root stem cell development and differentiation in plants. The study of At ROW1 in plant may provide an ideal model for further elucidating the functional mechanism of BARD1 in mammals.

关键词： repressor of Wuschel-1 DNA repair stem cell niche

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自噬抑制剂促进紫杉醇耐药卵巢癌细胞对化疗敏感性的研究

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实用癌症杂志 2024年第2期39卷 181-185页

作者：杨璇张玉洲郭靖河南省开封市妇产医院 475000

目的探讨自噬激活在紫杉醇(paclitaxel,PTX)耐药卵巢癌(ovarian cancer)细胞中的作用及自噬抑制对耐药细胞化疗的影响。方法以卵巢癌细胞系SKOV3和紫杉醇耐药卵巢癌细胞(SKOV3/PTX)为研究对象;利用MTT实验检测细胞活力变化;Annexin V/P... 详细信息

目的探讨自噬激活在紫杉醇(paclitaxel,PTX)耐药卵巢癌(ovarian cancer)细胞中的作用及自噬抑制对耐药细胞化疗的影响。方法以卵巢癌细胞系SKOV3和紫杉醇耐药卵巢癌细胞(SKOV3/PTX)为研究对象;利用MTT实验检测细胞活力变化;Annexin V/PI双染法检测细胞凋亡;免疫印迹(Western blot,WB)法检测蛋白表达变化;利用脂质体瞬时转染法使细胞表达LC3-GFP-RFP荧光蛋白;利用激光共聚焦显微镜检测细胞荧光蛋白定位及表达。结果相比SKOV3细胞,相同剂量的PTX对SKOV3/PTX细胞的活力抑制能力明显降低,紫杉醇对SKOV3细胞和SKOV3/PTX细胞的半数抑制浓度((inhibitory concentration 50%,IC50)分别为(41.91±2.92)μM和(114.6±17.6)μM。此外,在凋亡水平上,PTX对SKOV3/PTX细胞的诱导凋亡作用显著降低。通过LC3-GFP-RFP过表达和LC3、p62蛋白检测,证明SKOV3/PTX细胞的自噬激活程度增高。采用自噬抑制剂氯喹(chloroquine,CQ)和3-MA可以阻断细胞自噬,并提高PTX对SKOV3/PTX细胞的活力抑制能力和诱导凋亡作用。结论自噬激活在紫杉醇耐药的卵巢癌细胞中发挥了保护作用,抑制自噬可以增强紫杉醇对耐药细胞的杀伤作用。

关键词：卵巢癌紫杉醇耐药细胞紫杉醇自噬 SKOV3

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Origin of a Subgenome and Genome Evolution of Allotetraploid Cotton Species

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Molecular Plant 2020年第9期13卷 1238-1240页

作者： Peng He yuzhou zhang Guanghui Xiao College of Life Sciences Shaanxi Normal UniversityXi'an 710119China Institute of Science and Technology(IST)Austria 3400 KlosterneuburgAustria

Cotton(Gossypium spp.)is one of the most important economic crops in the world and also a major source of natural fiber,oil,and *** morphology of cotton species varies from trailing herbaceous perennials to trees<10 *** other important crops,modern cotton cultivars are polyploids and have gone through polyploidization,evolution,and *** cotton genus comprises approximately 45 diploid(2n=2x=26)and seven tetraploid species(2n=4x=52)(Guan et al.,2014).

关键词： crops trail cotton

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Multi-Agent System for Electric Vehicle Charging Scheduling in Parking Lots

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Complex System Modeling and Simulation 2023年第2期3卷 129-142页

作者： Mao Tan Zhonglin zhang Yuling Ren Irampaye Richard yuzhou zhang School of Automation and Electronic Information Xiangtan UniversityXiangtan 411105China School of Computer Science&School of Cyberspace Science Xiangtan UniversityXiangtan 411105China Hunan National Center for Applied Mathematics Xiangtan UniversityXiangtan 411105China Hunan Hi-Tech New Energy Co.Ltd. Changsha 410006China.

As the number of electric vehicles(EVs)increases,massive numbers of EVs have started to gather in commercial parking lots to charge and discharge,which may significantly impact the operation of the *** may also be a deviation in the departure time of charged and discharged EVs in commercial parking *** deviation can lead to insufficient battery energy when the EVs leave the parking *** study uses the simulation software AnyLogic to build a commercial parking lot multi-agent simulation model,and the agent-based model can fully reflect the autonomy of individual *** on this simulation model,we propose an EV scheduling *** algorithm contains two main *** first is the power distribution center agent(PDCA),which is used to coordinate the energy output of photovoltaic(PV),energy storage system(ESS),and distribution station(DS)to solve the problem of grid *** second is the scheduling center agent(SCA),which is used to solve the insufficient battery energy problem due to EVs’random *** SCA includes two *** the first stage,a priority scheduling algorithm is proposed to emphasize the fairness of EV *** the second stage,a genetic algorithm is used to accurately determine the time interval between charging and discharging to ensure the maximum benefit of EV ***,simulation experiments are conducted in AnyLogic,and the results demonstrate the superiority of the algorithm over the classical algorithm.

关键词： electric vehicle charging scheduling multi-agent system simultation optimization

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