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NIR-Ⅱ Excitation and NIR-I Emission Based Two-photon Fluorescence Lifetime Microscopic Imaging Using Aggregation-induced Emission Dots

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Chemical Research in Chinese Universities 2021年第1期37卷 171-176页

作者： LIU Wen ZHANG yuhuang QI Jj QIAN Jun TANG Ben Zhong State Key Laboratory of Modern Optical Instrumentations Centre for Optical and Electromagnetic ResearchCollege of Optical Science and EngineeringInternational Research Center for Advanced PhotonicsZhejiang UniversityHangzhou310058P.R.China Guangdong Provincial Key Laboratory of Luminescence from Molecular Aggregates South China University of TechnologyGuangzhou510640P.R.China Department of Chemistry The Hong Kong Branch of Chinese National Engineering Research Center for Tissue Restoration and ReconstructionInstitute for Advanced Study and Department of Chemical and Biological Engineering and Institute of Molecular Functional MaterialsThe Hong Kong University of Science and TechnologyClear Water BayKowloonHong KongP.R.China

Near-infrared(NIR)lights are powerful tools to conduct deep-tissue imaging since NIR-Ⅰ wavelengths hold less photon absorption and NIR-Ⅱ wavelengths serve low photon scattering in the biological tissues compared with visible ***-photon fluorescence lifetime microscopy(2PFLM)can utilize NIR-Ⅱ excitation and NIR-Ⅰ emission at the same time with the assistance of a well-designed fluorescent *** induced emission(AIE)dyes are famous for unique optical properties and could serve a large two-photon absorption(2PA)cross-section as aggregated ***,we report two-photon fluorescence lifetime microscopic imaging with NIR-Ⅱ excitation and NIR-Ⅰ emission using a novel deep-red AIE *** AIE-gens held a 2PA cross-section as large as 1.61×10^(4)GM at 1040 *** AIE dots had a two-photon fluorescence peak at 790 nm and a stable lifetime of 2.2 ns under the excitation of 1040 nm femtosecond *** brain vessels of a living mouse were vividly reconstructed with the two-photon fluorescence lifetime information obtained by our home-made 2PFLM *** vessels as small as 3.17µm were still observed with a nice signal-background ratio at the depth of 750µ*** work will inspire more insight into the improvement of the working wavelength of fluorescent agents and traditional 2PFLM.

关键词： Near-infrared Brain imaging Aggregation-induced emission Two-photon fluorescence lifetime microscopic imaging

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Effects of Fat-soluble Extracts From Vegetable Powder and β-carotene on Proliferation and Apoptosis of Lung Cancer Cell YTMLC-90

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Biomedical and Environmental Sciences 2003年第3期16卷 237-245页

作者： QUAN-JUNLU CHENG-yuhuang SHU-XIANGYAO RUI-SHUWANG XIAO-NAWU DepartmentofNutritionandFoodHygiene CollegeofPublicHealthZhengzhouUniversityZhengzhou450052HenanChina DepartmentofNutritionandFoodHygiene WestChinaSchoolofPublicHealthSichuanUniversityChengdu610041SichuanChina YunnanTinMinerCorporation GejiuCity661400YunnanChina DepartmentofClinicalNutrition People‘sHospitalofSichuanProvinceChengdu610031SichuanChina

Objective The aim of this investigation was to study the effects of fat-soluble extracts from vegetable powder (FEFVP) and P-carotene on the proliferation and apoptosis of cultured YTMLC-90 lung cancer cells. Methods The lung cancer cells were continuously exposed to a broad range of concentration of FEFVP and P-carotene. The proliferation was evaluated in MTT test. The induction of apoptosis was evaluated by morphological change, DNA fragmentation analysis, and DNA content analysis combined with flow cytometric analysis. Results Both FEFVP and P-carotene were found to inhibit cell proliferation and to induce morphologic changes consistent with apoptosis in YTMLC-90 cancer cells, including cellular shrinkage, chromatin condensation and nuclear fragmentation. DNA agarose gel electrophoresis showed DNA fragmentation 'ladder'. Flow cytometric analysis revealed decreased DNA content and the presence of a sub-G1 apoptotic peak. Conclusion These findings are consistent with the induction of apoptosis. Moreover, the effects of FEFVP are stronger than those of P-carotene. FEFVP inhibits the growth of YTMLC-90 probably via the induction of apoptosis cancer cells.

关键词： Cell culture FEFVP Apoptosis

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ROBUST STABILITY ANALYSIS BY TRANSFORMATION OF YOULA PARAMERIZATION

ROBUST STABILITY ANALYSIS BY TRANSFORMATION OF YOULA PARAMER...

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计算机，通信，控制与电力工程国际会议

作者： Qiu yuhuang Ji Hongbin Institute of Automation Academia Sinica

ISBN: (纸本)780003285X

This paper addre（?） an alternative approack to analyze nominal and robunt stability via Youla *** parameter,Q,which is used to form all nornimally stabili zing controllers,can then be interpreted as a feedback compensator in the transformed framework of this ***, we can purposefully design the parameter Q to achieve Hoptimal *** addition,the constructive procedure is provided to form the standard plant in the presence of *** condition of robuat stability is also obtained for the uncertain systems.

关键词： ROBUST STABILITY ANALYSIS BY TRANSFORMATION OF YOULA PARAMERIZATION

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Characteristics and quality of clinical practice guidelines for diabetic kidney disease:a systematic review

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Journal of Traditional Chinese Medicine 2024年第3期44卷 609-619页

作者： WANG yuhuang ZHANG Le ZHANG Zhengshan YAO Zhi LI Xiyao SUN Luying LIAO Xing Department of Nephropathy and Endocrine Dongzhimen HospitalBeijing University of Chinese MedicineBeijing 100010China Centre for Evidence-Based Chinese Medicine Institute of Basic Research in Clinical MedicineChina Academy of Chinese Medical SciencesBeijing 100010China Department of Endocrine One Zone Fangshan HospitalBeijing University of Chinese MedicineBeijing 102400China

OBJECTIVE:To assess the quality of Clinical practice guidelines(CPGs)in the context of diabetic kidney disease(DKD)and determine whether any factors affect the ***:We searched eight databases along with five international and national organizations to develop or archive guidelines from their inception to July 2023,with an additional search of *** the authoritative organizations related to *** and consensus statements created using direct differential diagnosis or therapy for DKD were included without language *** quality was evaluated by four reviewers using the Appraisal of Guidelines for Research and EvaluationⅡ(AGREEⅡ)*** with the item and domain scores,the guideline was also allocated an overall quality score,which ranged from 1(lowest possible quality)to 7(highest possible quality).Moreover,an overall recommendation for use was also assigned(“recommended”,“recommended with modifications”or“not recommended”).RESULTS:A total of 16 CPGs were included,of which 14 were from Asia and the remaining two from *** two CPGs were updated in the third *** CPGs were recommended for use because their primary domains scored in the medium or high ***,five CPGs were recommended with modifications as the stakeholder involvement,applicability,and editorial independence domains were evaluated as low *** all domains,the lowest average score was for rigour of development(33%),followed by application(36%),and stakeholder involvement(51%).The highest average score was for scope and purpose(79%),followed by clarity of presentation(75%).None of the CPGs considered the patient's viewpoint,and six of 16 CPGs did not use any grading system to translate the evidence into ***,only three of 16 CPGs shared search strategy,and eight of 16 CPGs did not declare a funding ***:According to the AGREE II evaluation,more than one in four CPGs for DKD had poo

关键词： diabetic nephropathies practice guideline appraisal of guidelines for research and evaluation II quality assessment systematic review

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EFFICIENT ANALYSIS OF ELECTROMAGNETIC RADIATION FROM MICROSTRIP ANTENNA USING VOLUME-SURFACE CURRENT CONTUNUITY METHOD

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Journal of Electronics(China) 2012年第6期29卷 567-571页

作者： Yuan Jiade Su Kaixiong Ye yuhuang College of Physics and Information Engineering Fuzhou University

The Volume-Surface Current Continuity Method (VSCCM) is presented to analyze electromagnetic radiation from microstrip antenna. The microstrip antenna is discretized into small triangular patches on conducting surface and tetrahedral volume cells in dielectric region. The Method of Moments (MoM) is applied to solve the integral equation. An equation contains the restriction relation between the volume and surface current coefficient is derived from the current continuity equation at those parts where the conducting surface is in contact with the dielectric material. A simple equivalent strip model is introduced in the treatment of the feeding probe in VSCCM. The VSCCM can reduce the unknowns required to be solved in MoM, as well as the condition number of the matrix equation. Numerical results are given to validate the accuracy and efficiency of this method.

关键词： Method of Moments (MoM) Volume-Surface Integral Equation (VSIE) Microstrip antenna Volume-Surface Current Continuity Method (VSCCM)

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Studies on Purification of Methamidophos Monoclonal Antibodies and Comparative Immunoactivity of Purified Antibodies

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Biomedical and Environmental Sciences 2003年第2期16卷 119-125页

作者： SU-QINGZHAO YUAN-MINGSUN CHUN-YANZHANG XIAO-yuhuang HOU-RuIZHANG ZHEN-YUZHU GuangxiInstituteofBotany GuangxiAutonomousRegionandAcademiaSinicaGuilin541006China SunYat-SenMedicalCollege SunYat-SenUniversityGuangzhou510642China ZhongshanMedicalSchool SunYat-Sen（Zhongshan）UniversityGuangzhou510089China

To purify Methamidophos (Met) monoclonal antibodies with two methods and compare immune activity of purified antibodies. Method Caprylic acid ammonium sulphate precipition (CAASP) method and Sepharose protein-A (SPA) affinity chromatography method were used to purify Met monoclonal antibodies, UV spectrum scanning was used to determine protein content and recovery of purified antibodies, sodium dodecylsulphate polyacrylamide gel electrophoresis (SDS-PAGE) was used to analyze the purity of purified antibodies, and enzyme-linked immunosorbent assay (ELISA) was used to determine immune activity of purified antibodies. Results Antibody protein content and recovery rate with CAASP method were 7.62 mg/mL and 8.05% respectively, antibody protein content and recovery rate with SPA method were 6.45 mg/mL and 5.52% respectively. Purity of antibodies purified by SPA method was higher than that by CAASP method. The half-maximal inhibition concentration (IC50) of antibodies purified by SPA to Met was 181.26 靏/mL, and the linear working range and the limit of quantification (LOD) were 2.43-3896.01 靏/mL and 1.03 靏/mL, respectively. The IC50 of antibodies purified by CAASP to Met was 352.82 靏/mL, and the linear working range and LOD were 10.91-11412.29 靏/mL and 3.42 靏/mL, respectively. Conclusion Antibodies purified by SPA method are better than those by CAASP method, and Met monoclonal antibodies purified by SPA method can be used to prepare gold-labelled testing paper for analyzing Met residue in vegetable and drink water.

关键词： Methamidophos Monoclonal antibody Purification Immunoactivity

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Vascular Actions of Tamoxifen and Raloxifene

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中国药理通讯 2005年第1期22卷 41-42页

作者： yuhuang DepartmentofPhysiology ChineseUniversityofHongKongChina

Cardiovascular disease is among the leadingcauses of mortality worldwide. A sexrelated difference exists in the incidence of cardiovascular diseases with increased risks of cardiovascular disease such as stroke after menopause. Previous observational studies show significant reductions in the risk of coronary heart disease among users of hormone replacement therapy (HRT) for the primary and secondary prevention of cardiovascular disease.

关键词：脉管作用三苯氧胺心血管疾病预防作用

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Designing Textile Architectures for High Energy-Efficiency Human Body Sweat-and Cooling-Management

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Advanced Fiber Materials 2019年第1期1卷 61-70页

作者： Kun Fu Zhi Yang Yong Pei Yongxin Wang Beibei Xu yuhuang Wang Bao Yang Liangbing Hu Department of Materials Science and Engineering University of MarylandCollege ParkMD 20742USA Department of Mechanical Engineering University of DelawareNewarkDE 19716USA Department of Mechanical Engineering University of MarylandCollege ParkMD 20742USA Department of Chemistry University of MarylandCollege ParkMD 20742USA

Thermal management of textiles requires local microclimate control over heat and wet dissipation to create a comfortable thermal-wet environment at the interface of the human body and ***,we design a fabric capable of both sweat-and cooling-management using a knitted fabric featuring a bilayer structure consisting of hydrophobic polyethylene terephthalate and hydrophilic cellulose fibers to simultaneously achieve high infrared(IR)transmittance and good thermal-wet *** IR transmission of this cooling textile increased by~twofold in the dry state and~eightfold in the wet state compared to conventional cotton *** the porosity changes from 10 to 47%with the comparison of conventional cotton fabric and our cooling textile,the heat flux is increased from 74.4 to 152.3 W/cm^(2).The cooling effect of the cooling fabric is 105%greater than that of commercial cotton fabric,which displays a better thermal management capacity for personal *** bilayer design controls fast moisture transfer from inside out and provides thermal management,demonstrating high impact not only for garments,but also for other systems requiring heat regulation,such as buildings,which could mitigate energy demand and ultimately contribute to the relief of global energy and climate issues.

关键词： Thermal responsive textile IR transmission Cooling textiles Moisture transfer and management

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Xia Boyu:The Chinese Hero Who Climbed Mount Qomolangma Without Legs

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China Book International 2024年第5期 126-128页

作者： Zheng yuhuang Zhang Mingming Tsinghua University World Marketing Hall of Fame in China Beijing Institute of Technology Macao University of Science and Technology

This book not only covers the essence of the author’s more than 20 years of self-control research but also includes the main research results in the field of global self-control in the past few decades,introduces the scientific principles behind self-control in detail,and provides scientific and effective self-control improvement strategies and a large number of vivid and interesting self-control improvement *** book is an easy-to-understand book for people who want to learn about self-control,and improve self-control.

关键词： Mount Who principles

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A COLLABORATIVE SPECTRUM SENSING SCHEME USING FUZZY COMPREHENSIVE EVALUATION IN COGNITIVE RADIO

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Journal of Electronics(China) 2009年第3期26卷 326-331页

作者： Yang Wendong Ye yuhuang Cai Yueming Xu Youyun Institute of Communications Engineering PLA University of Science and Technology Nanjing 210007 China Institute of Microwave Communication Fuzhou University Fuzhou 350002 China

Cognitive Radio(CR) is a promising technology to solve the challenging spectrum scarcity ***, to implement CR, spectrum sensing is the groundwork and the *** this paper, a collaborative spectrum sensing scheme using fuzzy comprehensive evaluation is *** final sensing decision of the proposed scheme is based on the combination of distributed sensing results of different Secondary Users(SUs).To improve the reliability of the sensing decision, the combination procedure takes into account the credibility of each SU, which is evaluated using fuzzy comprehensive *** effect of the presence of malicious SUs and malfunctioning SUs on the performance of the proposed scheme is also *** efficiency of the scheme is validated through analysis and simulation.

关键词： Cognitive Radio (CR) Collaborative spectrum sensing Fuzzy comprehensive evaluation

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