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Experiment of drag characteristics of a novel variable geometry flame-holder

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航空动力学报 2011年第11期26卷 2485-2494页

作者： LIU yang JIN Jie WANG Hui-ru LIU yu-ying yang Mao-lin School of Jet Propulsion Beijing University of Aeronautics and AstronauticsBeijing 100191China

A novel variable geometry flame-holder(VGF) based on V-gutter was *** structural characteristics of the flame holder were *** study on drag characteristics of the VGF on un-burning and burning states was conducted in a rectangular combustion test *** influence of the change of gutter trailing edge width w on pressure recovery in different operating conditions was analyzed ***,drag characteristics in different trailing edge width variation modes were *** show that:(1) Narrowing w can observably raise the total pressure recovery σ but the highest σ is not obtained at minimum w.(2) The relationship of drag coefficient ψ and w in the un-burning conditions can be well expressed in a form of exponential equation(w/d>0.4).(3) Drag characteristics are not sensitive to the variation modes and speeds of w.(4) In the burning condition,σ decreases with the increasing of w basically and the course can be divided into three *** results and experimental data presented in this paper would enhance the phenomenological understanding of this type of VGF,and would contribute to the next experimental study and numerical calculus of this novel VGF.

关键词： flame-holder variable geometry afterburner augmentor drag characteristics

Biodegradable Polypeptide-based Vesicles with Intrinsic Blue Fluorescence for Antibacterial Visualization

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Chinese Journal of Polymer Science 2021年第11期39卷 1412-1420页

作者： yu-ying yang Ling-Shan Chen Min Sun Cong-yu Wang Zhen Fan Jian-Zhong Du Department of Orthopedics Shanghai Tenth People's HospitalTongji UniversityShanghai 200072China Department of Polymeric Materials School of Materials Science and EngineeringTongji UniversityShanghai 201804China Institute for Advanced Study Tongji UniversityShanghai 200092China

Fluorescence imaging has been an indispensable tool to provide dynamic information about the localization and quantity of ***,due to the intrinsic hollow structure and modularized biofunctionalities,polymer vesicles have been widely applied in biomedical ***,most polymer vesicles are embedded with organic fluorophores for fluorescence imaging,which have certain drawbacks such as leakage and possible ***,we present a biodegradable polypeptide-based vesicle with intrinsic blue fluorescence without introducing any fluorophore for real-time visualization of antibacterial *** modular design to integrate multiple functional fragments,poly(ε-caprolactone)-block-poly(tryptophan)-block-poly(lysine-stat-phenylalanine)[PCL25-b-PTrp2-b-P(Lys13-statPhe4)]was synthesized,where PCL chains form the hydrophobic membrane,P(Lys-stat-Phe)and PTrp provide intrinsic fluorescence and broadspectrum antibacterial *** is noteworthy that the fluorescence emission was shifted from invisible ultraviolet range of amino acids to visible range(emission maximum at 436 nm),which makes it possible to visualize the antibacterial *** addition,through utilizing the intrinsic fluorescence of vesicles,confocal fluorescent imaging of vesicles with bacteria validated the specific adhesion of vesicle towards bacteria,and the bacterial death through membrane ***,we provided a novel approach to developing biodegradable fluorescent polypeptide-based vesicles for real-time visualization of antibacterial process.

关键词： Polypeptide-based vesicles Intrinsic fluorescence Antibacterial visualization

The apple 14-3-3 protein MdGRF11 interacts with the BTB protein MdBT2 to regulate nitrate deficiency-induced anthocyanin accumulation

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Horticulture Research 2021年第1期8卷 265-278页

作者： Yi-Ran Ren Qiang Zhao yu-ying yang Tian-En Zhang Xiao-Fei Wang Chun-Xiang You yu-Jin Hao State Key Laboratory of Crop Biology Shandong Collaborative Innovation Center for Fruit and Vegetable Production with High Quality and EfficiencyCollege of Horticulture Science and EngineeringShandong Agricultural UniversityTai-AnShandong 271018China Qingdao Agricultural University QingdaoShandong 266109China

Nitrogen is an important factor that affects plant anthocyanin *** apple,the nitrate-responsive BTB/TAZ protein MdBT2 negatively regulates anthocyanin *** this study,we found that MdBT2 undergoes posttranslational modifications in response to nitrate *** two-hybrid,protein pull-down,and bimolecular fluorescence complementation(BiFC)assays showed that MdBT2 interacts with MdGRF11,a 14-3-3 protein;14-3-3 proteins compose a family of highly conserved phosphopeptide-binding proteins involved in multiple physiological and biological *** interaction of MdGRF11 negatively regulated the stability of the MdBT2 protein via a 26S proteasome-dependent pathway,which increased the abundance of MdMYB1 proteins to activate the expression of anthocyanin biosynthesis-related *** together,the results demonstrate the critical role of 14-3-3 proteins in the regulation of nitrate deficiency-induced anthocyanin *** results provide a novel avenue to elucidate the mechanism underlying the induction of anthocyanin biosynthesis in response to nitrate deficiency.

关键词： nitrate GRF BTB

Clinical significance of EPHX2 deregulation in prostate cancer

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Asian Journal of Andrology 2021年第1期23卷 109-115页

作者： Ming-Sheng Liu Hui Zhao Chen-Xiang Xu Ping-Bo Xie Wei Wang ying-yu yang Wen-Hui Lee yang Jin Hong-Qing Zhou The Second Ward of Urology Qujing Affiliated Hospital of Kunming Medical UniversityQujing 655000China Department of Urology The First Affiliated Hospital of Kunming Medical UniversityKunming 650332China Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences Kunming Institute of ZoologyChinese Academy of SciencesKunming 650223China Institute for Cancer Genetics and Informatics Oslo University HospitalOslo 0379Norway

The arachidonic acid(AA)metabolic pathway participates in various physiological processes as well as in the development of *** analyzed genomic alterations in AA metabolic enzymes in the Cancer Genome Atlas(TCGA)prostate cancer(PCa)dataset and found that the gene encoding soluble epoxide hydrolase(EPHX2)is frequently deleted in ***2 mRNA and protein expression in PCa was examined in multiple datasets by differential gene expression analysis and in a tissue microarray by *** expression data were analyzed in conjunction with clinicopathological *** the mRNA and protein expression levels of EPHX2 were significantly decreased in tumors compared with normal prostate tissues and were inversely correlated with the Gleason grade and disease-free survival ***,EPHX2 mRNA expression was significantly decreased in metastatic and recurrent PCa compared with localized and primary PCa,*** addition,EPHX2 protein expression correlated negatively with Ki67 *** conclusion,EPHX2 deregulation is significantly correlated with the clinical characteristics of PCa progression and may serve as a prognostic marker for PCa.

关键词： arachidonic acid metabolism metastasis prognosis prostate cancer soluble epoxide hydrolase

Expression of Endogenous Retrovirus ev/J gp85 Gene and Analysis of Its Immunoreactivity in Comparison with Exogenous Viral Protein

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Virologica Sinica 2008年第5期23卷 369-377页

作者： yu-ying yang Ai-jian QIN Xiong-yan LIANG Shu-mei TONG Colloge of Veterinary Medicine Yangzhou University Yangzhou 225009 China College of Animal Science Yangtzeu University Jingzhou 434023 China

The envelope gene gp85 of ev/J, a new family of endogenous avian retroviral sequences identified recently, has the most extensive nucleotide sequence identity ever described with ALV-J avian leukosis virus. This report described expression of ev/J envelope gene gp85 derived from commercial meat-type chicken using the Invitrogen Bac-to-Bac baculovirus expression system. The antigenicity and immunoreactivity of the recombinant endogenous gp85 gene product (SU) were analyzed by indirect immunofluorescence, Western blot, indirect and blocking Enzyme-Linked ImmunoSorbent Assay (ELISA) using JE9 monoclonal antibody (MAb) against the envelope protein of ALV-J (ADOL-4817), positive mouse antiserum against the ev/J gp85 SU and sera from chicken naturally infected with ALV-J. The results showed that the ev/J gp85 SU can bind specifically to JE9 MAb and antiserum from chicken naturally infected with ALV-J, and the binding reactivity between exogenous ALV-J gp85 SU and natural positive chicken serum against exogenous ALV-J can be blocked by positive mouse serum against the ev/J gp85 SU. It is concluded that recombinant endogenous gp85 gene product (SU) has close immunological relatedness to the envelope protein of exogenous ALV-J (ADOL-4817 and IMC10200 strain).

关键词： Avian endogenous retrovirus ev/J Envelope gene gp85 Expression Immunoreactivity

Antidiabetic effect of a novel non-thiazolidinedione PPAR γ/α agonist on ob/ob mice

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Acta Pharmacologica Sinica 2006年第10期27卷 1346-1352页

作者： Xi HU ying FENG yu SHEN Xiao-feng ZHAO Juan-hong yu yu-she yang ying LENG~2 Shanghai Institute of Materia Medica,Shanghai Institute for Biological Sciences,Chinese Academy of Sciences,Shanghai 201203,China Shanghai Institute of Materia Medica Shanghai Institute for Biological Sciences Chinese Academy of Sciences Shanghai 201203 China

Aim:To study whether T33,a new synthesized non-thiazolidinedione(TZD)per-oxisome proliferator-activated receptor(PPAR)γ/α dual agonist has an antidia-betic effect on ob/ob ***:Ob/ob mice were treated with 4 mg/kg or 8mg/kg T33 by gavage for 20 *** glucose levels were measured *** glucose tolerance test(OGTT)and an insulin tolerance test(ITT)were pre-formed on d 8 and d 12,*** levels of insulin,triglyceride and freefatty acid(FFA)in the serum were measured at the end of *** and liver triglyceride content was also ***:T33reduced the hyperglycemia,hyperinsulinemia and hyperlipidemia of the ob/*** OGTT and ITT showed that the insulin resistance state of the ob/obmice was obviously ameliorated after T33 *** 20 d treatment with 8mg/kg T33,the triglyceride content in the gastrocnemius muscle decreasedsignificantly.T33 did not have any effect on triglyceride content in the liver,whereas rosiglitazone significantly increased the hepatocyte lipid ***:The PPARγ/α dual agonist T33 has antidiabetic and insulin-sensitiz-ing effects in ob/ob *** has the potential to be a new therapeutic candidate forthe treatment of type 2 diabetes.

关键词： diabetes insulin-sensitizing peroxisome proliferator-activated receptors thiazolidinediones

新藤黄酸对宫颈癌HeLa细胞增殖、凋亡、迁移的影响及机制研究

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中药材 2016年第6期39卷 1393-1398页

作者：石玉荣杨滢蚌埠医学院安徽蚌埠233000

目的:研究新藤黄酸对宫颈癌HeLa细胞增殖、凋亡、迁移的影响及其机制。方法:不同浓度新藤黄酸(0.00、0.63、1.25、2.50、5.00、10.00 mg/L)作用于HeLa细胞72 h,MTT法检测细胞增殖;流式细胞术检测细胞周期;荧光显微镜和DNA ladder检测细... 详细信息

目的:研究新藤黄酸对宫颈癌HeLa细胞增殖、凋亡、迁移的影响及其机制。方法:不同浓度新藤黄酸(0.00、0.63、1.25、2.50、5.00、10.00 mg/L)作用于HeLa细胞72 h,MTT法检测细胞增殖;流式细胞术检测细胞周期;荧光显微镜和DNA ladder检测细胞凋亡;Transwell实验检测细胞迁移;RT-PCR、Western blot检测BCL-2、BAX、NF-κB及E-cadherin的mRNA和蛋白表达。结果:不同浓度新藤黄酸作用HeLa细胞72 h后,细胞抑制率随药物浓度升高而升高;穿过Transwell小室的细胞数随药物浓度升高而减少;BCL-2、NF-κB mRNA及蛋白表达随新藤黄酸浓度升高而降低;BAX、E-cadherin mRNA及蛋白表达随新藤黄酸浓度的升高而升高。结论:新藤黄酸在一定范围内能以剂浓度依赖性抑制HeLa细胞增殖,促进其凋亡,并抑制其迁移。

关键词：宫颈癌 HeLa细胞新藤黄酸增殖凋亡迁移

Adiponectin Receptor 1 Single Nucleotide Polymorphism Is Highly Associated with Hypertriglyceridemia in Asian Male—A Novel Genetic Screening to Reduce Risk of Cerebrovascular Disease

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World Journal of Neuroscience 2015年第5期5卷 323-327页

作者： William C. W. Huang Rong-Fu Chen yu-ying yang Department of Family Medicine Show-Chwan Memorial Hospital Taiwan Department of Medical Research and Development Show-Chwan Memorial Hospital Taiwan Department of Clinical Laboratory Show-Chwan Memorial Hospital Taiwan

Background: Adiponectin is involved in regulating both glucose and fatty acid. Associations of the known adiponectin receptors 1 (ADIPOR1) single nucleotide polymorphism (SNP) with diabetes have been demonstrated while hypertriglyceridemia is frequently associated with cerebrovascular disease (CVD) among diabetes. Triglyceride metabolism was also reported to be different between genders and estrogen was observed to interfere with adiponectin effects via ADIPOR1. It seems important to investigate whether the ADIPOR1 SNP variants may be significant determinants in triglyceride metabolism and hence be a risk of CVD in specific gender. Methods: A survey was performed on random self-reported healthy subjects aged 35 and above with their biochemical data collected. Genotyping for ADIPOR1 SNP (rs1342387) was carried out using?TaqMan Genotyping Assays. Interviews were also conducted regarding stress, adverse diet behavior and exercise. Multivariable logistic regression analyses were performed to identify the strongest contributing variables. Findings: The ADIPOR1 minor allele carrier (T/T and T/C) had significantly (p?= 0.02) higher TG mean compared to homozygous (C/C) major alleles. TG difference was significantly higher in male (p?= 0.02) with a larger difference in mean, whereas the difference disappeared among female (p?= 0.32). Multivariate logistic regression analyses were performed by defining abnormal TG based on NCEP criteria of metabolic syndrome, and when all life style variables were entered in the model with ADIPOR1, only the ADIPOR1 inmale showed significant (p?= 0.03) and very high association with abnormal TG (Exp(β) 16.31). Discussion: The findings provide sample evidence of a relation between ADIPOR1 SNP minor allele carrier and high TG concentrations in male. The association of abnormal TG and ADIPOR1 is much stronger than that compared to life style. The implications of this survey may be further extend to identifying the genetic risk of abnormal TG at y

关键词： ADIPOR1 SNP Hypertriglyceridemia Male

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RNA修饰类型及调控蛋白

生命科学 2018年第4期30卷 391-406页

作者：陈宇晟杨莹中国科学院北京基因组研究所精准基因组医学重点实验室北京100101

RNA修饰是指发生在RNA上的各种修饰形式。自然界中的RNA修饰广泛存在于A、U、C、G四类核苷酸上,此外,极少的RNA修饰发生在次黄嘌呤核苷(I)上。目前已经在古细菌、细菌、病毒和真核生物中发现超过140种的RNA转录后修饰形式。在各种类型的... 详细信息

RNA修饰是指发生在RNA上的各种修饰形式。自然界中的RNA修饰广泛存在于A、U、C、G四类核苷酸上,此外,极少的RNA修饰发生在次黄嘌呤核苷(I)上。目前已经在古细菌、细菌、病毒和真核生物中发现超过140种的RNA转录后修饰形式。在各种类型的RNA修饰中,甲基化修饰占到了三分之二,这些修饰广泛存在于各种RNA类型中,包括信使RNA(mRNA)、转运RNA(tRNA)、核糖体RNA(rRNA)、核内小RNA(snRNA)、核仁小RNA(sno RNA)、微小RNA(miRNA)、小干扰RNA(siRNA)、piwi蛋白相互作用的RNA(piRNA)和长非编码RNA(lncRNA)等。现介绍主要的RNA修饰类型,并对其调控蛋白进行归纳总结。

关键词： RNA修饰 RNA甲基化 N^6-甲基腺嘌呤 5-甲基胞嘧啶甲基转移酶去甲基化酶结合蛋白