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丙戊酸钠诱导Siha细胞周期阻滞的实验观察

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中华肿瘤防治杂志 2009年第11期16卷 834-836页

作者：李娜于世英武汉大学人民医院肿瘤科湖北武汉430060 华中科技大学附属同济医院肿瘤中心湖北武汉430030

目的:研究丙戊酸钠对人宫颈癌Siha细胞增殖和细胞周期的影响。方法:MTT检测生长抑制,流式细胞仪检测细胞周期,蛋白质印迹法检测p21WAF1/CIP1、p-CDK2和cyclin E蛋白。结果:丙戊酸钠以剂量依赖性方式抑制Siha细胞生长IC20和IC50分别为2.0... 详细信息

目的:研究丙戊酸钠对人宫颈癌Siha细胞增殖和细胞周期的影响。方法:MTT检测生长抑制,流式细胞仪检测细胞周期,蛋白质印迹法检测p21WAF1/CIP1、p-CDK2和cyclin E蛋白。结果:丙戊酸钠以剂量依赖性方式抑制Siha细胞生长IC20和IC50分别为2.0 mmol/L和8.0 mmol/L;丙戊酸钠上调G0/G1期比例由(74.92±0.60)%升高至(84.475±1.11)%,P=0.001,下调S期(13.19±0.70)%降为(7.72±1.40)%,P=0.004和G2/M(11.89±1.30)%降为(7.81±0.31)%期比例,P=0.006。丙戊酸钠上调p21WAF1/CIP1蛋白,下调cyclin E和p-CDK2蛋白。结论:丙戊酸钠上调p21WAF1/CIP1表达和抑制cyclin E表达,使细胞阻滞于G0/G1期,抑制Siha细胞生长。

关键词：丙戊酸钠宫颈肿瘤细胞周期 p21WAF1/CIP1 cyclin E

Highly efficient labelling of extracellular vesicles for enhanced detection on a microfluidic platform

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Chinese Chemical Letters 2024年第2期35卷 441-445页

作者： shi Hu Rui Hao Zitong yu Huitao Zhang Hui Yang Bionic Sensing and Intelligence Center Institute of Biomedical and Health EngineeringShenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhen 518055China

Developing precise extracellular vesicles(EVs)labelling techniques with minimal disturbance is of great importance to the follow-up EVs detection and ***,currently available methods such as using probes to conjugate phospholipids or membrane proteins have certain limitations due to EV steric hindrance,dye aggregation,***,we present a microfluidic platform to enhance EVs’labelling efficiency and improve their *** platform provides excellent sample throughput and high-efficiency EV labelling at lower label concentrations with an optimized flowing *** cytometry analysis(FCM)and cellular uptake results show that EV labelling by utilizing this platform possesses the merits of a higher labelling efficiency with 64.1%relative improvement than conventional co-incubation method and a lower background ***,this technique maintains EVs’size,morphology and biological *** the recipient cells uptake the EVs treated by the microfluidic platform,the spatial and temporal distribution of EVs in the cells are clearly *** results demonstrate that our method holds great potential in efficient labelling of EVs,which is essential to subsequent EV quantification and analysis.

关键词： Microfluidic platform Extracellular vesicles Labelling PKH dye Flow cytometry

Numerical design of an efficient Ho^(3+)-doped InF3 fiber laser at~3.2μm

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Journal of Electronic Science and Technology 2024年第3期22卷 35-46页

作者： shi-yuan Zhou Hong-yu Luo Ya-Zhou Wang Yong Liu State Key Laboratory of Electronic Thin Films and Integrated Devices School of Optoelectronic Science and EngineeringUniversity of Electronic Science and Technology of ChinaChengdu611731China DTU Electro Technical University of DenmarkKongens Lyngby2800Denmark

In this work,we theoretically unlock the potential of Ho^(3+)-doped InF3 fiber for efficient~3.2μm laser generation(from the 5F4,5S2→5F5 transition),by employing a novel dual-wavelength pumping scheme at 1150 nm and 980 nm,for the first *** clad-coupled 1150 nm pumping of 5 W,~3.2μm power of 3.6 W has been predicted with the optical-to-optical efficiency of 14.4%.Further efficient power scaling,however,is blocked by the output saturation with 980 nm *** alleviate this behavior,the cascaded 5I5→5I6 transition,targeting~3.9μm,has been activated simultaneously,therefore accelerating the population circulation between the laser upper level 5F4,5S2 and long-lived 5I6 level under 980 nm *** a result,enhanced~3.2μm power of 4.68 W has been obtained with optical-to-optical efficiency of 15.6%.Meanwhile the~3.9μm laser,yielding power of 2.76 W with optical-to-optical efficiency of 9.2%,is theoretically achievable as well with a moderate heat load,of which the performance is even better than the prior experimentally and theoretically reported Ho^(3+)-doped InF3 fiber lasers emitting at~3.9μm *** work demonstrates a versatile platform for laser generation at~3.2μm and~3.9μm,thus providing the new opportunities for many potential applications,e.g.,polymer processing,infrared countermeasures,and free-space communications.

关键词： Cascaded Fiber laser Holmium Mid-infrared

A real-time intelligent lithology identification method based on a dynamic felling strategy weighted random forest algorithm

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Petroleum Science 2024年第2期21卷 1135-1148页

作者： Tie Yan Rui Xu shi-Hui Sun Zhao-Kai Hou Jin-yu Feng School of Petroleum Engineering Northeast Petroleum UniversityDaqing163318HeilongjiangChina Sanya Offshore Oil&Gas Research Institute Northeast Petroleum UniversitySanya572025HainanChina

Real-time intelligent lithology identification while drilling is vital to realizing downhole closed-loop drilling. The complex and changeable geological environment in the drilling makes lithology identification face many challenges. This paper studies the problems of difficult feature information extraction,low precision of thin-layer identification and limited applicability of the model in intelligent lithologic identification. The author tries to improve the comprehensive performance of the lithology identification model from three aspects: data feature extraction, class balance, and model design. A new real-time intelligent lithology identification model of dynamic felling strategy weighted random forest algorithm(DFW-RF) is proposed. According to the feature selection results, gamma ray and 2 MHz phase resistivity are the logging while drilling(LWD) parameters that significantly influence lithology identification. The comprehensive performance of the DFW-RF lithology identification model has been verified in the application of 3 wells in different areas. By comparing the prediction results of five typical lithology identification algorithms, the DFW-RF model has a higher lithology identification accuracy rate and F1 score. This model improves the identification accuracy of thin-layer lithology and is effective and feasible in different geological environments. The DFW-RF model plays a truly efficient role in the realtime intelligent identification of lithologic information in closed-loop drilling and has greater applicability, which is worthy of being widely used in logging interpretation.

关键词： Intelligent drilling Closed-loop drilling Lithology identification Random forest algorithm Feature extraction

A direct electrochemical biosensor for rapid glucose detection based on nitrogen-doped carbon nanocages

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Rare Metals 2024年第5期43卷 2184-2192页

作者： Lan-Lan Li yu Zhao Li-Jia Pan Jian-Bin Xu Yi shi College of Mechanical and Electrical Engineering Henan Agricultural UniversityZhengzhou 450002China School of Electronic Science and Engineering Collaborative Innovation Center of Advanced MicrostructuresNanjing UniversityNanjing210093China Department of Electronic Engineering The Chinese University of HongKongHongkong 999077China

Given the increasing number of diabetic patients,rapid and accurate detection of glucose in body fluids is *** study developed a direct electrochemical biosensor for glucose based on nitrogen-doped carbon nanocages(NCNCs).NCNCs possess a large specific surface area of 1395 m^(2)·g^(-1),a high N atomic content of 9.37%and good biocompatibility,which is favorable for enzyme loading and electron *** surface average concentration of electroactive glucose oxidase on NCNCs was 2.82×10^(-10)mol·cm^(-2).The NCNC-based direct electrochemical biosensor exhibited a high sensitivity of 13.7μA·(mmol·L^(-1))^(-1)·cm^(-2),rapid response time of 5 s and an impressive electron-transferrate constant(ks)of 1.87 s^(-1).Furthermore,we investigated an NCNC-based direct electron transfer(DET)biosensor for sweat glucose detection,which demonstrated tremendous promise for non-invasive wearable diabetes diagnosis.

关键词： Direct electron transfer(DET) Nitrogen-doped carbon nanocages(NCNCs) Glucose oxidase Biosensor

LncFASA promotes cancer ferroptosis via modulating PRDX1 phase separation

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Science China(Life Sciences) 2024年第3期67卷 488-503页

作者： Xiao Fan Fangzhou Liu Xiang Wang ying Wang yu Chen Chengyu shi Xinwan Su Manman Tan Qingfeng Yan Jinrong Peng Jianzhong Shao Yan Xiong Aifu Lin MOE Laboratory of Biosystem Homeostasis and Protection College of Life SciencesZhejiang UniversityHangzhou 310058China Key Laboratoryof Cancer Prevention and Intervention China National Ministry of EducationHangzhou 310009China Cancer Center Zhejiang UniversityHangzhou 310058China Key Laboratory for Cell and Gene Engineering of Zhejiang Province Hangzhou 310058China Department of Central Laboratory the First People's Hospital of HuzhouHuzhou 313000China Key Laboratory of Integrated Oncology and Intelligent Medicine of Zhejiang Province Affiliated Hangzhou First People's HospitalZhejiang University School of MedicineHangzhou 310006China MOE Key Laboratory for Molecular Animal Nutrition College of Animal SciencesZhejiang UniversityHangzhou 310058China Department of Orthopedic Surgery the Second Affiliated HospitalSchool of MedicineZhejiang UniversityHangzhou 310000China Breast Center of the First Affiliated Hospital School of MedicineZhejiang UniversityHangzhou 310003China International School of Medicine International Institutes of MedicineThe 4th Affiliated Hospital of Zhejiang University School of MedicineYiwu 322000China

Ferroptosis, a unique type of non-apoptotic cell death resulting from iron-dependent lipid peroxidation, has a potential physiological function in tumor suppression, but its underlying mechanisms have not been fully elucidated. Here, we report that the long non-coding RNA(lncRNA) LncFASA increases the susceptibility of triple-negative breast cancer(TNBC) to ferroptosis. As a tumor suppressor, LncFASA drives the formation of droplets containing peroxiredoxin1(PRDX1), a member of the peroxidase family, resulting in the accumulation of lipid peroxidation via the SLC7A11-GPX4 axis. Mechanistically, LncFASA directly binds to the Ahpc-TSA domain of PRDX1, inhibiting its peroxidase activity by driving liquid-liquid phase separation, which disrupts intracellular ROS homeostasis. Notably, high LncFASA expression indicates favorable overall survival in individuals with breast cancer, and LncFASA impairs the growth of breast xenograft tumors by modulating ferroptosis. Together, our findings illustrate the crucial role of this lncRNA in ferroptosis-mediated cancer development and provide new insights into therapeutic strategies for breast cancer.

关键词： long non-coding RNA peroxiredoxin liquid-liquid phase separation ferroptosis breast cancer

Robust tantalum tuned perovskite oxygen electrode for reversible protonic ceramic electrochemical cells

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Rare Metals 2024年第7期43卷 3055-3065页

作者： yu-shi Feng yu Shen Fang Wang Chuan Tian Qin-Yi Hu Qian Duan Jing-Wei Li Di Bao School of Materials Science and Engineering Changchun University of Science and TechnologyChangchun 130022China Zhongshan Institute of Changchun University of Science and Technology Zhongshan528437China State Key Laboratory of Rare Earth Resource Utilization Changchun Institute of Applied Chemistry Chinese Academy of SciencesChangchun 130022China Key Laboratory of Preparation and Applications of Environmental Friendly Materials Ministry of EducationJilin Normal UniversityChangchun 130103China

Perovskite oxides with diverse composition and structure have exhibited grand advances in boosting the oxygen reduction and evolution reaction(ORR/OER),which are essential for the reversible protonic ceramic electrochemical cell(R-PCEC)toward the sustainable hydrogen production and ***,enhancement of their activity and stability remains ***,we develop the Ta-regulated BaCo_(0.7)Fe_(0.3)O_(3-δ)perovskite oxygen electrode(Ba(Co_(0.7)Fe_(0.3))_(1-x)Ta_xO_(3-δ))with abundant oxygen defects and achieve the simultaneous enhancement in the electrocatalytic activity and stability toward ORR and ***-fabricated R-PCEC with(Ba(Co_(0.7)Fe_(0.3))_(0.9)Ta_(0.1)O_(3-δ))(BCFT10)oxygen electrode performs high power density of 1.47 W·cm^(-2)at 650℃in fuel cell mode,and the current density is up to-2.11 A·cm^(-2)at 1.4 V at 650℃in electrolysis mode,as well as the good stability in both the fuel cell and electrolysis ***,the cell also demonstrates a stable cycling operation between fuel cell and electrolysis mode,suggesting a great potential of BCFT10 as oxygen electrode material for R-PCECs.

关键词： Reversible protonic ceramic electrochemical cells Perovskite oxide Oxygen electrode Ta element doping Oxygen defects

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移动社交支付采纳行为影响因素实证研究

统计与信息论坛 2015年第8期30卷 92-99页

作者：余世英邢亚楠武汉大学信息管理学院湖北武汉430072

以技术接受模型为基础,以移动社交支付应用为研究对象,根据研究情景进行模型拓展,加入网络外部性、感知娱乐性、社会影响三个变量,根据变量间的关系构建研究模型并使用SmartPLS软件检验。研究表明,产品感知(感知有用性、易用性、娱乐性... 详细信息

以技术接受模型为基础,以移动社交支付应用为研究对象,根据研究情景进行模型拓展,加入网络外部性、感知娱乐性、社会影响三个变量,根据变量间的关系构建研究模型并使用SmartPLS软件检验。研究表明,产品感知(感知有用性、易用性、娱乐性)、社会影响和网络外部性是影响用户行为的重要因素;网络外部性与用户感知(感知有用性、易用性、娱乐性)和行为意向正相关;社会影响对感知有用性、感知易用性、网络外部性和行为意向影响显著;感知易用性与感知娱乐性正相关。

关键词：移动社交支付技术接受模型网络外部性感知娱乐性社会影响结构方程模型

《国际功能、残疾和健康分类》乳腺癌核心功能组合评定乳腺癌患者生活质量的可行性研究

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中华物理医学与康复杂志 2010年第1期32卷 44-46页

作者：钟易于世英武汉市中心医院肿瘤科武汉430042 华中科技大学同济医学院附属同济医院肿瘤科

目的评价《国际功能、残疾和健康分类》（ICF）乳腺癌核心功能组合在中国乳腺癌患者中使用的可行性。方法采用ICF乳腺癌核心功能组合及简明健康测量量表（SF-36）评估110例乳腺癌患者的生活质量，比较两种量表的可信性、有效性及可行性... 详细信息

目的评价《国际功能、残疾和健康分类》（ICF）乳腺癌核心功能组合在中国乳腺癌患者中使用的可行性。方法采用ICF乳腺癌核心功能组合及简明健康测量量表（SF-36）评估110例乳腺癌患者的生活质量，比较两种量表的可信性、有效性及可行性。其中被测者信度、克朗巴赫系数、内部一致性信度、分半信度反映量表可信性，准则关联效度、结构效度反映量表有效性，量表完成率及完成时间反映量表可行性。结果ICF乳腺癌核心功能组合具有较好的可信性、较高的有效性和符合量表要求的可行性。结论ICF乳腺癌核心功能组合适用于中国乳腺癌患者。

关键词：乳腺癌生活质量量表可信性有效性可行性