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Low Light Stress Down-Regulated Rubisco Gene Expression and Photosynthetic Capacity During Cucumber (Cucumis sativus L.) Leaf Development

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Journal of Integrative Agriculture 2014年第5期13卷 997-1007页

作者： SUN Jian-lei SUI xiao-lei HUANG Hong-yu WANG Shao-hui wei yu-xia ZHANG Zhen-xian Beijing Key Laboratory of Growth and Development Regulation for Protected Vegetable Crops/College of Agriculture and Biotechnology China Agricultural University Plant Science Department Beijing University of Agriculture

Low light stress is one of the most important factors affecting photosynthesis and growth in winter production of cucumber （Cucumis sativus L.） in solar greenhouses in northern China. Here, two genotypes of cucumber （Deltastar and Jinyan 2） are used to determine the effect of low light stress on Rubisco expression and photosynthesis of leaves from emergence to senescence. During leaf development, the net photosynthetic rate （PN）, stomatal conductance （gs）, Rubisco initial activity and activation state, transcript levels of rbcL and rbcS, and the abundance of rbcL and rbcS DNA in these two genotypes increase rapidly to reach maximum in 10-20 d, and then decrease gradually. Meanwhile, the actual photosystem II efficiency （OpSll） of cucumber leaves slowly increased in the early leaf developing stages, but it declined quickly in leaf senescent stages, accompanied by an increased non-photochemical quenching （NPQ）. Moreover, PN, gs, initial Rubisco activity, and abundance of protein, mRNA and DNA of Rubisco subunits of leaves grown under 100 μmol m^-2 s^-1 are lower, and require more time to reach their maxima than those grown under 600 μmol m^-2 s^-1 during leaf development. All these results suggest that lower photosynthetic capacity of cucumber leaves from emergence to senescence under low light stress is probably due to down-regulated Rubisco gene expression in transcript and protein levels, and decreased initial and total activity as well as activation state of Rubisco. Deltastar performs better than Jinyan 2 under low light stress.

关键词： cucumber photosynthetic capacity Rubisco rbcL rbcS low light

Splenic subcapsular hematoma following endoscopic retrograde cholangiopancreatography:A case report and review of literature

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World Journal of Clinical Cases 2024年第24期12卷 5613-5621页

作者： Chen-yu Guo yu-xia wei Department of Gastroenterology Inner Mongolia Autonomous Region Hospital of Traditional Chinese MedicineHohhot 010000Inner Mongolia Autonomous RegionChina Graduate School Inner Mongolia Medical UniversityHohhot 010000Inner Mongolia Autonomous RegionChina

BACKGROUND Splenic injury following endoscopic retrograde cholangiopancreatography(ERCP)is a rare *** literature contains around 30 articles reporting various degrees of splenic injuries resulting from ERCP since the first report of splenic rupture after ERCP in *** SUMMARY This report describes a case of splenic hematoma and stent displacement in a 69-year-old male patient who developed these conditions 7 days after undergoing ERCP and *** patient had bile duct stenosis caused by a malignant tumor that was obstructing the bile *** diagnosis was confirmed by epigastric computed tomography and magnetic resonance *** patient was successfully treated with percutaneous transhepatic cholangial drainage,endoscopic pyloric stent placement,and conservative *** causes of splenic injury following ERCP are *** ERCP has the potential to cause splenic *** a patient experiences symptoms such as abdominal pain,decreased blood pressure,and altered hematology after the procedure,it's important to be thoroughly investigated for postoperative bleeding and splenic injury.

关键词： Endoscopic retrograde cholangiopancreatography Gastroenterology Splenic injury Hematoma Case report

Roles of Fas signaling pathway in vitamin E succinateinduced apoptosis in human gastric cancer SGC-7901 cells

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World Journal of Gastroenterology 2002年第6期8卷 982-986页

作者： Kun Wu Yao Li Yan Zhao yu-Juan Shah wei xia Lan Zhao,Department of Nutrition and Food Hygiene,Public Health School,Harbin Medical University,Harbin 150001,Heilongjiang Province,China wei-Ping yu,Genetics Institute,Texas University of USA,Austin,USA Department of Nutrition and Food Hygiene Public Health School Harbin Medical University Heilongjiang Province China. wukun@***

AIM: To investigate the roles of Fas signaling pathway in vitamin E succinate-induced apoptosis in human gastric cancer SGC-7901 ***: Human gastric cancer SGC-7901 cells were treated with VES at 5, 10, 20 mg@L-1, succinic acid and vitamin E as vehicle control and condition media only as untreated (UT) control. Apoptotic morphology was observed by DAPI staining. Western blot analysis was applied to measure the expression of Fas, FADD and caspase-8 proteins. After the cells were transiently transfected with Fas and FADD antisense oligonucleotides, respectively, caspase-8 activity was determined by flurometric ***: The morphologically apoptotic changes were observed after VES treatment by DAPI staining. 23.7 % and 89.6 % apoptosis occurred after 24 h and 48 h of 20 mg@L-1 VES treatment, respectively. The protein levels of Fas, FADD and caspase-8 were evidently increased in a dose-dependent manner after 24 h of VES treatment. The blockage of Fas by transfection with Fas antisense oligonucleotides obviously inhibited the expression of FADD protein. After SGC-7901 cells were transfected with Fas and FADD antisense oligonucleotides, caspase-8 activity was obviously decreased (P＜0.01), whereas Fas blocked more than ***: VES-induced apoptosis in human gastric cancer SGC-7901 cells involves Fas signaling pathway including the interaction of Fas, FADD and caspase-8.

关键词：人胃癌细胞 SGC-7091细胞系 Ve琥珀酸酯维生素E 诱发凋亡 Fas信号通路作用机制

Radiomics and nomogram of magnetic resonance imaging for preoperative prediction of microvascular invasion in small hepatocellular carcinoma

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World Journal of Gastroenterology 2022年第31期28卷 4399-4416页

作者： Yi-Di Chen Ling Zhang Zhi-Peng Zhou Bin Lin Zi-Jian Jiang Cheng Tang Yi-Wu Dang yu-wei xia Bin Song Li-Ling Long Department of Radiology The First Affiliated Hospital of Guangxi Medical UniversityNanning 530021Guangxi Zhuang Autonomous RegionChina Department of Radiology Affiliated Hospital of Guilin Medical UniversityGuilin 541001Guangxi Zhuang Autonomous RegionChina Department of Pathology The First Affiliated Hospital of Guangxi Medical UniversityNanning 5350021Guangxi Zhuang Autonomous RegionChina Department of Technology Huiying Medical Technology(Beijing)Beijing 100192China Department of Radiology West China HospitalSichuan UniversityChengdu 610041Sichuan ProvinceChina Key Laboratory of Early Prevention and Treatment for Regional High Frequency Tumor Ministry of EducationGuangxi Medical UniversityNanning 530021Guangxi Zhuang Autonomous RegionChina Guangxi Key Laboratory of Immunology and Metabolism for Liver Diseases First Affiliated Hospital of Guangxi Medical UniversityNanning 530021Guangxi Zhuang Autonomous RegionChina

BACKGROUND Microvascular invasion(MVI)of small hepatocellular carcinoma(sHCC)(≤3.0 cm)is an independent prognostic factor for poor progression-free and overall *** can help extract imaging information associated with tumor *** To develop and validate radiomics scores and a nomogram of gadolinium ethoxybenzyl-diethylenetriamine pentaacetic acid(Gd-EOB-DTPA)-enhanced magnetic resonance imaging(MRI)for preoperative prediction of MVI in *** In total,415 patients were diagnosed with sHCC by postoperative pathology.A total of 221 patients were retrospectively included from our *** addition,we recruited 94 and 100 participants as independent external validation sets from two other *** models of Gd-EOB-DTPA-enhanced MRI and diffusion-weighted imaging(DWI)were constructed and validated using machine *** presented in the radiomics nomogram,a prediction model was developed using multivariable logistic regression analysis,which included radiomics scores,radiologic features,and clinical features,such as the alpha-fetoprotein(AFP)*** calibration,decision-making curve,and clinical usefulness of the radiomics nomogram were *** radiomic nomogram was validated using independent external cohort *** areas under the receiver operating curve(AUC)were used to assess the predictive *** Pathological examination confirmed MVI in 64(28.9%),22(23.4%),and 16(16.0%)of the 221,94,and 100 patients,***,tumor size,non-smooth tumor margin,incomplete capsule,and peritumoral hypointensity in hepatobiliary phase(HBP)images had poor diagnostic value for MVI of *** radiomic features(1409)of MRI scans)were *** classifier of logistic regression(LR)was the best machine learning method,and the radiomics scores of HBP and DWI had great diagnostic efficiency for the prediction of MVI in both the testing set(hospital A)and validation set(hospital B,C).The AUC of HBP was

关键词： Magnetic resonance imaging Hepatocellular carcinoma Radiomics Nomogram

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四溴荧光素与DNA作用的研究

光谱学与光谱分析 2006年第1期26卷 125-129页

作者：卞伟魏玉霞卫艳丽董川山西医科大学基础医学院山西太原030001 山西大学化学化工学院山西太原030006

用荧光光谱和磷光光谱法研究了四溴荧光素(TBF)与DNA的相互作用。探讨了盐效应和乙醇在DNA存在和不存在条件下对TBF体系荧光强度的影响,以及不同pH在DNA存在和不存在条件下对体系磷光强度的影响。由荧光猝灭实验,得到无DNA存在下的荧光... 详细信息

用荧光光谱和磷光光谱法研究了四溴荧光素(TBF)与DNA的相互作用。探讨了盐效应和乙醇在DNA存在和不存在条件下对TBF体系荧光强度的影响,以及不同pH在DNA存在和不存在条件下对体系磷光强度的影响。由荧光猝灭实验,得到无DNA存在下的荧光猝灭常数为719.74L·mol-1,有DNA存在下的荧光猝灭常数为880.22L·mol-1。并通过荧光猝灭实验和磷光偏振实验得出了TBF和DNA的可能作用方式。

关键词：四溴荧光素脱氧核糖核酸荧光光谱法磷光光谱法

Effects of buried penis on the structure and function of corpus cavernosum in a rat model

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Chinese Medical Journal 2010年第13期123卷 1736-1740页

作者： CHENG Fan yu wei-min xia yue ZHANG xiao-bin YANG Si-xing GE Ming-huan Department of Urology Renmin Hospital of Wuhan University Wuhan Hubei 430060 China

Background While the abnormal appearance of the concealed penis has been well recognized, the effect of buried penis on the structure and function of corpus cavernosum has not been well studied. To explore this issue, we established a rat model and evaluated the effect of buried penis on cavernosum weight, contents and ultrastructure of tissue, and nitric oxide synthase （NOS） activity. Methods Two hundred and ten rats were randomly divided into 3 equal cohorts for 2, 4 and 6 months study （groups A, B and C）. Each group was randomly divided into buried group （n=40）, control group （n=15）, and normal group （n=15）, respectively. Intra-purse-string suture of the root of the penis was used to establish the model. Macroscopic development was judged by measuring the weight of the corpus cavernosum. Masson＇s trichrome staining was performed for observing microstructure while a transmission electron microscope was used for observing ultrastructure. The NOS activity was detected by a NOS activity assay kit. Results Buried penis had no significant influence on the appearance and weight of the corpus cavernosum. Buried penis resulted in decreased smooth muscle content （P 〉0.05 in group A, and P 〈0.05 in groups B and C） and increased fibrous connective tissue content （P 〉0.05 in groups A and B, and P 〈0.05 in group C） compared with the normal and control groups. Ultrastructural abnormalities of corpus cavernosum were observed in the 6-month buried group. Moreover, there was decrease of NOS activity in groups B and C （P 〈0.05 in group B and P 〈0.01 in group C） when compared with the normal and control groups. Conclusion Buried penis affects the structure and function of corpus cavernosum in rats and the effect is positively correlated with the buried time, but there is no significant effect on the macroscopic development.

关键词： buried penis corpus cavernosum structure function

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某难选菱铁矿石直接压球及还原焙烧

过程工程学报 2011年第5期11卷 794-799页

作者：魏玉霞孙体昌寇珏余文北京科技大学金属矿山高效开采与安全教育部重点实验室北京100083

将某难选菱铁矿石破碎到粒度小于2mm后,加入一定量粘结剂和内配煤与矿石压制成型后再还原焙烧,通过对成球条件、直接还原焙烧过程影响因素和磨矿磁选条件进行实验,确定了最佳条件,最终可得到铁品位92.76%、回收率为85.97%的直接还原铁产... 详细信息

将某难选菱铁矿石破碎到粒度小于2mm后,加入一定量粘结剂和内配煤与矿石压制成型后再还原焙烧,通过对成球条件、直接还原焙烧过程影响因素和磨矿磁选条件进行实验,确定了最佳条件,最终可得到铁品位92.76%、回收率为85.97%的直接还原铁产品.结果表明,粒度小于2mm的菱铁矿石压球后直接还原可得到高品位、高回收率直接还原铁,还原过程中球未发生粘结、碎裂、膨胀等现象,还原状态良好.

关键词：菱铁矿石压球还原焙烧直接还原铁

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二苯并噻吩脱硫微生物菌种的筛选与活性

中国环境科学 2006年第3期26卷 311-314页

作者：魏玉霞肖宝清闫海简明宇北京科技大学应用科学学院北京100083 北京科技大学土木与环境工程学院北京100083

从被石油污染的土壤中筛选出1株能对二苯并噻吩(DBT)进行高效脱硫的微生物菌种,初步鉴定为红串红球菌USTB-03(RhodococcuserythropolisUSTB-03).该菌种可以按特异性脱硫途径(简称4S途径)将DBT转化为2-羟基联苯(2HBP)和亚硫酸作为最终脱... 详细信息

从被石油污染的土壤中筛选出1株能对二苯并噻吩(DBT)进行高效脱硫的微生物菌种,初步鉴定为红串红球菌USTB-03(RhodococcuserythropolisUSTB-03).该菌种可以按特异性脱硫途径(简称4S途径)将DBT转化为2-羟基联苯(2HBP)和亚硫酸作为最终脱硫产物.在葡萄糖、甘油和乙醇分别作为微生物生长的唯一碳源时,葡萄糖是支持该菌生长和提高其脱硫比活性的较好碳源,使培养出的微生物对DBT的脱硫比活性达到了68.63mmol2HBP/(kg·h).该菌株还可以对4,6-二甲基二苯并噻吩(4,6-DMDBT)进行脱硫.

关键词：二苯并噻吩(DBT) 红串红球菌USTB-03 生物脱硫

Identifying medication regularity of traditional Chinese medicine and potential pharmacological mechanism of Jiedu Sangen decoction in colorectal cancer treatment by data mining and network pharmacology

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Gastroenterology & Hepatology Research 2023年第3期5卷 12-28页

作者： Xin-Ru Jia xiang-Chang Ying yu-wei xia Li-Hui Qian Lei-Tao Sun Pei-Pei Wang The First School of Clinical Medicine Zhejiang Chinese Medical UniversityHangzhou 310053China Department of Medical Oncology The First Affiliated Hospital of Zhejiang Chinese Medical University(Zhejiang Provincial Hospital of Chinese Medicine)Hangzhou 310006China Institute of Zhejiang Chinese Medical Culture Zhejiang Chinese Medical UniversityHangzhou 310053China Academy of Chinese Medical Science Zhejiang Chinese Medical UniversityHangzhou 310053China Zhejiang Cancer Hospital Hangzhou Institute of Medicine(HIM)Chinese Academy of SciencesHangzhou 310022China

Objective:Colorectal cancer(CRC)is one of the most common malignancies in the world,and traditional Chinese medicine(TCM)is widely used in its treatment in ***,the medication rules of TCM for CRC treatment remain ***,data mining combined with network pharmacology was utilized to establish treatment principles and ***:The CRC cases treated at Zhejiang Provincial Hospital of Chinese Medicine from January 1,2016 to October 31,2021 were analyzed using data mining ***-Q/TOF-MS analysis was performed to identify the chemical composition of Jiedu Sangen decoction(JSD).Network pharmacology was used to reveal the therapeutic mechanism of ***:A total of 312 cases 2,998 prescriptions that met the inclusion criteria used 343 kinds of traditional Chinese *** nature of the herbs used in treatment was mainly warm and *** taste was mainly sweet,bitter,and *** meridian tropisms were mainly the spleen meridian,followed by lung and stomach *** and replenishing herbs were the most frequently used in ***-frequency herbs were classified into 11 categories by cluster analysis,and 42 association rules were obtained by association rule *** with complex network analysis,3 core prescriptions for clinical CRC treatment were *** Sangen decoction contains 64 chemical ingredients,out of which 31 active ingredients were identified,including polydatin,caffeic acid,and glutamic acid,along with 130 potential targets such as AKT1,SRC,and *** Sangen decoction may play a role in regulating inflammation,immunity,metabolism,and hormones in the development of CRC via pathways such as the relaxin signaling pathway,IL-17 signaling pathway,prolactin signaling pathway,and T cell receptor signaling ***:This study summarizes the treatment and medication principles for clinical CRC treatment,promoting the inheritance and development of the traditional Chinese medica

关键词： data mining network pharmacology traditional Chinese medicine colorectal cancer Jiedu Sangen decoction