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Changes in proteins related to early nerve repair in a rat model of sciatic nerve injury

Neural Regeneration Research 2021年第8期16卷 1622-1627页

作者： yu-song yuan Fei yu Ya-Jun Zhang Su-Ping Niu Hai-Lin Xu yu-Hui Kou Department of Trauma and Orthopedics Peking University People’s HospitalBeijingChina Key Laboratory of Trauma and Neural Regeneration(Peking University) Ministry of EducationBeijingChina National&Local Joint Engineering Research Center of Orthopedic Biomaterials Department of Bone&Joint SurgeryPeking University Shenzhen HospitalShenzhenGuangdong ProvinceChina National Center for Trauma Medicine BeijingChina Office of Academic Research Peking University People’s HospitalBeijingChina Diabetic Foot Treatment Center Peking University People’s HospitalBeijingChina

Peripheral nerves have a limited capacity for self-repair and those that are severely damaged or have significant defects are challenging to repair. Investigating the pathophysiology of peripheral nerve repair is important for the clinical treatment of peripheral nerve repair and regeneration. In this study, rat models of right sciatic nerve injury were established by a clamping method. Protein chip assay was performed to quantify the levels of neurotrophic, inflammation-related, chemotaxis-related and cell generation-related factors in the sciatic nerve within 7 days after injury. The results revealed that the expression levels of neurotrophic factors(ciliary neurotrophic factor) and inflammationrelated factors(intercellular cell adhesion molecule-1, interferon γ, interleukin-1α, interleukin-2, interleukin-4, interleukin-6, monocyte chemoattractant protein-1, prolactin R, receptor of advanced glycation end products and tumor necrosis factor-α), chemotaxis-related factors(cytokine-induced neutrophil chemoattractant-1, L-selectin and platelet-derived growth factor-AA) and cell generation-related factors(granulocyte-macrophage colony-stimulating factor) followed different trajectories. These findings will help clarify the pathophysiology of sciatic nerve injury repair and develop clinical treatments of peripheral nerve injury. This study was approved by the Ethics Committee of Peking University People's Hospital of China(approval No. 2015-50) on December 9, 2015.

关键词： animal model cell generation chemotaxis clamp injury inflammation injury neurotrophic factor peripheral nerve protein array repair sciatic nerve Wallerian degeneration

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Brain functional remodeling caused by sciatic nerve transposition repair in rats identified by multiplemodel resting-state blood oxygenation level-dependent functional magnetic resonance imaging analysis

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Neural Regeneration Research 2022年第2期17卷 418-426页

作者： yu-song yuan Hai-Lin Xu Zhong-Di Liu yu-Hui Kou Bo Jin Pei-Xun Zhang Department of Trauma and Orthopedics Peking University People’s HospitalPeking UniversityBeijingChina Key Laboratory of Trauma and Neural Regeneration(Peking University) Ministry of EducationBeijingChina National Center for Trauma Medicine BeijingChina

Lower extremity nerve transposition repair has become an important treatment strategy for peripheral nerve injury;however, brain changes caused by this surgical procedure remain unclear. In this study, the distal stump of the right sciatic nerve in a rat model of sciatic nerve injury was connected to the proximal end of the left sciatic nerve using a chitin conduit. Neuroelectrophysiological test showed that the right lower limb displayed nerve conduction, and the structure of myelinated nerve fibers recovered greatly. Muscle wet weight of the anterior tibialis and gastrocnemius recovered as well. Multiple-model resting-state blood oxygenation level-dependent functional magnetic resonance imaging analysis revealed functional remodeling in multiple brain regions and the re-establishment of motor and sensory functions through a new reflex arc. These findings suggest that sciatic nerve transposition repair induces brain functional remodeling. The study was approved by the Ethics Committee of Peking University People's Hospital on December 9, 2015(approval No. 2015-50).

关键词： brain connectivity brain network fALFF MRI nerve transposition repair peripheral nerve injury ReHo remodeling

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Combining CUBIC Optical Clearing and Thy1-YFP-16 Mice to Observe Morphological Axon Changes During Wallerian Degeneration

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Current Medical Science 2021年第5期41卷 944-952页

作者： yu-song yuan Fei yu Su-ping NIU Hao LU yu-hui KOU Hai-lin XU Department of Trauma and Orthopaedics Peking University People's HospitalPeking University'Beijing 100044China National and Local Joint Engineering Research Center of Orthopaedic Biomaterials Department of Bone&Joint SurgeryPeking University Shenzhen HospitalShenzhen 518036China Key Laboratory of Trauma and Neural Regeneration(Peking University) Ministry of EducationBeijing 100044China Office of Academic Research Peking University People's HospitalPeking UniversityBeijing 100044China Diabetic Foot Treatment Center Peking University People's HospitalPeking University'Beijing 100044China

Objective:Wallerian degeneration is a pathological process closely related to peripheral nerve regeneration following injury,and includes the disintegration and phagocytosis of peripheral nervous system ***,morphological changes are observed by performing immunofluorescence staining after sectioning,which results in the loss of some histological *** purpose of this study was to explore a new,nondestmetive,and systematic method for observing axonal histological changes during Wallerian ***:Thirty male Thy1-YFP-16 mice(SPF grade,6 weeks old,20±5 g)were randomly selected and divided into clear,unobstructed brain imaging cocktails and computational analysis(CUBIC)optical clearing(n=15)and traditional method groups(n=15).Five mice in each group were sacrificed at 1st,3rd,and 5th day following a crush *** histological axon changes were observed by CUBIC light optical clearing treatment,direct tissue section imaging,and HE ***:The results revealed that,compared with traditional imaging methods,there was no physical damage to the samples,which allowed for three-dimensional and deep-seated tissue imaging through *** image information could be nicely obtained by direct fluorescence imaging and HE staining,but it was difficult to obtain image information of the entire *** the same time,the image information obtained by fluorescence imaging and HE staining was partially ***:The combining of CUBIC and Thy1-YFP transgenic mice allowed for a clear and comprehensive observation of histological changes of axons in Wallerian degeneration.

关键词： Wallerian degeneration axon optical clearing clear,unobstructed brain imaging cocktails and computational analysis Thy1-YFP transgenic mice

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Synovial sarcoma in the plantar region: A case report and literature review

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World Journal of Clinical Cases 2019年第17期7卷 2549-2555页

作者： Jie Gao yu-song yuan Ting Liu Hao-Run Lv Hai-Lin Xu Pingxiang Health Vocational College Pingxiang Medical SchoolPingxiang 337000Jiangxi ProvinceChina Department of Trauma and Orthopedics Peking University People’s HospitalBeijing 100044China

BACKGROUND Synovial sarcoma (SS),a rare malignant soft tissue tumor whose histological origin is still unknown,often occurs in limbs in young people and is easily *** SUMMARY We report a 24-year-old man who sought treatment for plantar pain thought to be caused by a foot injury that occurred 4 years ***,he had been seen at another hospital for a 1-wk history of unexplained pain in the left plantar region and was treated with acupuncture,a kind of therapy of Chinese medicine,which partly relieved the *** of this,the final diagnosis of biphasic SS was made after two subsequent treatments by pathological evaluation after the last *** is rarely seen in the plantar area,and his history of a left plantar injury confused the original *** This study shows that pathological and imaging examinations may play a vital role in the early diagnosis and treatment of SS.

关键词： Imaging examination Pathological examination Plantar Synovial sarcoma Case report

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Intraoperative single administration of neutrophil peptide 1 accelerates the early functional recovery of peripheral nerves after crush injury

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Neural Regeneration Research 2020年第11期15卷 2108-2115页

作者： yu-song yuan Su-Ping Niu Fei yu Ya-Jun Zhang Na Han Hao Lu Xiao-Feng Yin Hai-Lin Xu yu-Hui Kou Department of Trauma and Orthopedics Peking University People’s HospitalPeking UniversityBeijingChina Key Laboratory of Trauma and Neural Regeneration(Peking University) Ministry of EducationBeijingChina Office of Academic Research Peking University People’s HospitalBeijingChina National and Local Joint Engineering Research Center of Orthopaedic Biomaterials Department of Bone&Joint SurgeryPeking University Shenzhen HospitalShenzhenGuangdong ProvinceChina National Center for Trauma Medicine BeijingChina Diabetic Foot Treatment Center Peking University People’s HospitalPeking UniversityBeijingChina

Neutrophil peptide 1 belongs to a family of peptides involved in innate immunity. Continuous intramuscular injection of neutrophil peptide 1 can promote the regeneration of peripheral nerves, but clinical application in this manner is not convenient. To this end, the effects of a single intraoperative administration of neutrophil peptide 1 on peripheral nerve regeneration were experimentally observed. A rat model of sciatic nerve crush injury was established using the clamp method. After model establishment, a normal saline group and a neutrophil peptide 1 group were injected with a single dose of normal saline or 10 μg/mL neutrophil peptide 1, respectively. A sham group, without sciatic nerve crush was also prepared as a control. Sciatic nerve function tests, neuroelectrophysiological tests, and hematoxylin-eosin staining showed that the nerve conduction velocity, sciatic functional index, and tibialis anterior muscle fiber cross-sectional area were better in the neutrophil peptide 1 group than in the normal saline group at 4 weeks after surgery. At 4 and 8 weeks after surgery, there were no differences in the wet weight of the tibialis anterior muscle between the neutrophil peptide 1 and saline groups. Histological staining of the sciatic nerve showed no significant differences in the number of myelinated nerve fibers or the axon cross-sectional area between the neutrophil peptide 1 and normal saline groups. The above data confirmed that a single dose of neutrophil peptide 1 during surgery can promote the recovery of neurological function 4 weeks after sciatic nerve injury. All the experiments were approved by the Medical Ethics Committee of Peking University People's Hospital, China(approval No. 2015-50) on December 9, 2015.

关键词： crush injury defensin 1 gait analysis intraoperative administration nerve conduction velocity nervous system neutrophil peptide 1 peripheral nerve injury peripheral nerve regeneration sciatic nerve tibialis anterior muscle trauma

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Burial depth interval of the shale brittle–ductile transition zone and its implications in shale gas exploration and production

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Petroleum Science 2017年第4期14卷 637-647页

作者： yu-song yuan Zhi-Jun Jin Yan Zhou Jun-Xin Liu Shuang-Jian Li Quan-You Liu Sinopec Petroleum Exploration and Production Research Institute 31 Xueyuan RoadBeijing 100083China Institute of Civil Engineering and Architecture Southwest University of Science and TechnologyMianyang 621002SichuanChina

Brittleness and ductility of shale are closely related to shale gas exploration and production. How to predict brittleness and ductility of shale is one of the key issues in the study of shale gas preservation and hydraulic fracturing treatments. The magnitude of shale brittleness was often determined by brittle mineral content(for example, quartz and feldspars) in shale gas ***, the shale brittleness is also controlled by burial depth. Shale brittle/ductile properties such as brittle, semibrittle and ductile can mutually transform with burial depth variation. We established a work flow of determining the burial depth interval of brittle–ductile transition zone for a given shale. Two boundaries were employed to divide the burial depth interval of shale brittle/ductile properties. One is the bottom boundary of the brittle zone(BZ), and the other is the top boundary of the ductile zone(DZ). The brittle–ductile transition zone(BDTZ) is between *** bottom boundary of BZ was determined by the overconsolidation ratio(OCR) threshold value combined with pre-consolidation stress which the shale experienced over geological time. The top boundary of DZ was determined based on the critical confining pressure of brittle–ductile transition. The OCR threshold value and the critical confining pressure were obtained from uniaxial strain andtriaxial compression tests. The BZ, DZ and BDTZ of the Lower Silurian Longmaxi shale in some representative shale gas exploration wells in eastern Sichuan and western Hubei areas were determined according to the above work flow. The results show that the BZ varies with the maximum burial depth and the DZ varies with the density of the overlying rocks except for the critical confining ***, the BDTZ determined by the above work flow is probably the best burial depth interval for marine shale gas exploration and production in Southern China. Shale located in the BDTZ is semi-brittle and is not prone to be severely n

关键词： Shale Brittleness Fracture Over-consolidation ratio(OCR) Confining pressure

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Repair of peripheral nerve defects by nerve transposition using small gap bio-sleeve suture with different inner diameters at both ends

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Neural Regeneration Research 2019年第4期14卷 706-712页

作者： yu-Hui Kou You-Lai yu Ya-Jun Zhang Na Han Xiao-Feng Yin yu-song yuan Fei yu Dian-Ying Zhang Pei-Xun Zhang Bao-Guo Jiang Peking University People's Hospital The Affiliated Hospital of Xuzhou Medical University

During peripheral nerve transposition repair, if the diameter difference between transposed nerves is large or multiple distal nerves must be repaired at the same time, traditional epineurial neurorrhaphy has the problem of high tension at the suture site, which may even lead to the failure of nerve suture. We investigated whether a small gap bio-sleeve suture with different inner diameters at both ends can be used to repair a 2-mm tibial nerve defect by proximal transposition of the common peroneal nerve in rats and compared the results with the repair seen after epineurial neurorrhaphy. Three months after surgery, neurological function, nerve regeneration, and recovery of nerve innervation muscle were assessed using the tibial nerve function index, neuroelectrophysiological testing, muscle biomechanics and wet weight measurement, osmic acid staining, and hematoxylin-eosin staining. There was no obvious inflammatory reaction and neuroma formation in the tibial nerve after repair by the small gap bio-sleeve suture with different inner diameters at both ends. The conduction velocity, muscle strength, wet muscle weight, cross-sectional area of muscle fibers, and the number of new myelinated nerve fibers in the biosleeve suture group were similar to those in the epineurial neurorrhaphy group. Our findings indicate that small gap bio-sleeve suture with different inner diameters at both ends can achieve surgical suture between nerves of different diameters and promote regeneration and functional recovery of injured peripheral nerves.

关键词： nerve regeneration bio-sleeve small gap sleeve suture nerve transposition nerve defect nerve conduit nerve reinnervation peripheral nerve neural regeneration

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Enhancement of monacolin K production via intergeneric protoplast fusion between Aspergillus terreus and Monascus anka

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Progress in Natural Science:Materials International 2007年第6期17卷 703-707页

作者： Chen Zhi, Lin Wen, yu Ping and song yuan(Department of Microbiology, College of Biological Sciences, China Agricultural University, Beijing 100094, China) Department of Microbiology College of Biological Sciences China Agricultural University Beijing 100094 China

Intergenric protoplast fusion between Aspergillus terreus CA99 and Monascus anka M-3, the high and low producers of monacolin K respectively,was performed for enhancement of monacolin K production. The 24-hour-old mycelia of A. terreus CA99 and M. anka M-3 were treated with 0.5% lywallzyme, 0.3% snailase and 0.3% cellulase at 34℃ for 5 h and at 30℃ for 3.5 h, and their protoplasts formation reached 1.76×107/mL and 1.68×107/mL respectively. Parental protoplasts were irradiated with a 30 W UV light away from 30 cm for 3 min and then mixed. The mixture was incubated with 30% PEG 6000 for 15 min. The reviving fusants were isolated on the regeneration plates. Of the 363 fusants isolated, over 100 showed enhanced monacolin K production compared with the parental strain M. anka M-3. Ten of them produced monacolin K about 1.6-fold of that *** M-3 does and the monacolin K titer of two fusants (F49 and F104) increased by about 1-fold. The monacolin K yields of F49 and F104 were 460 μg/mL and 457 μg/mL respectively. In optimized fermentation medium, the monacolin K titer of F49 reached 1216 μg/mL.

关键词： monacolin K Monascus anka Aspergillus terreus protoplast fusion fermentation.

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Reinnervation of spinal cord anterior horn cells after median nerve repair using transposition with other nerves

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Neural Regeneration Research 2019年第4期14卷 699-705页

作者： yu-song yuan Su-Ping Niu You-Lai yu Pei-Xun Zhang Xiao-Feng Yin Na Han Ya-Jun Zhang Dian-Ying Zhang Hai-Lin Xu yu-Hui Kou Bao-Guo Jiang Peking University People's Hospital The Affiliated Hospital of Xuzhou Medical University

Our previous studies have confirmed that during nerve transposition repair to injured peripheral nerves, the regenerated nerve fibers of motor neurons in the anterior horn of the spinal cord can effectively repair distal nerve and target muscle tissue and restore muscle motor function. To observe the effect of nerve regeneration and motor function recovery after several types of nerve transposition for median nerve defect(2 mm), 30 Sprague-Dawley rats were randomly divided into sham operation group, epineurial neurorrhaphy group, musculocutaneous nerve transposition group, medial pectoral nerve transposition group, and radial nerve muscular branch transposition group. Three months after nerve repair, the wrist flexion test was used to evaluate the recovery of wrist flexion after regeneration of median nerve in the affected limbs of rats. The number of myelinated nerve fibers, the thickness of myelin sheath, the diameter of axons and the cross-sectional area of axons in the proximal and distal segments of the repaired nerves were measured by osmic acid staining. The ratio of newly produced distal myelinated nerve fibers to the number of proximal myelinated nerve fibers was calculated. Wet weights of the flexor digitorum superficialis muscles were measured. Muscle fiber morphology was detected using hematoxylin-eosin staining. The cross-sectional area of muscle fibers was calculated to assess the recovery of muscles. Results showed that wrist flexion function was restored, and the nerve grew into the distal effector in all three nerve transposition groups and the epineurial neurorrhaphy group. There were differences in the number of myelinated nerve fibers in each group. The magnification of proximal to distal nerves was 1.80, 3.00, 2.50, and 3.12 in epineurial neurorrhaphy group, musculocutaneous nerve transposition group, medial pectoral nerve transposition group, and radial nerve muscular branch transposition group, respectively. Nevertheless, axon diameters of new

关键词： nerve regeneration transposition repair median nerve functional remodeling muscle atrophy surgical intervention peripheral nerve injury neural regeneration

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Evaluation and analysis of influencing factors on the quality of carbon disclosure:An empirical study of CDP in China

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Ecological Economy 2014年第2期7卷 144-158页

作者： song yu-yuan ZHANG Qiao-liang School of Economics and Management Lanzhou University of Technology

The purpose of this paper is to evaluate the quality of corporate carbon disclosure and to analyze its influencing factors. With the hand-collected carbon information from annual reports, social responsibility reports, sustainability reports and opened CDP questionnaires of the sample of 100 Chinese companies investigated by CDP from 2008-2011, the quality of carbon disclosure is defined by evaluating indicators obtained from factor analysis, and its influencing factors are studied from three aspects including company characteristics, financial features and corporate governance by means of correlation, differences comparison and regression analyses. Findings show that the quality of Chinese companies' carbon disclosure is mainly affected by company characteristics(such as the emission characteristics of one's industry and dual listed) and corporate governance(such as ownership concentration), but in perspective of financial features, there seems to be little relation between the disclosure quality and financing motivations. The practical implication of the paper is to help Chinese companies find out effective ways to improve carbon disclosure quality. The paper contributes to carbon disclosure literature by explaining the connotation of carbon information, providing a set of evaluation indicators of carbon disclosure and exploring the influencing factors from various perspectives.

关键词： quality of carbon disclosure evaluation index company characteristics financial features corporate governance

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