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Transcriptome responses to elevated CO2 level and Wolbachia-lniecWon stress in Hylyphantes graminicola (Araneae: Linyphiidae)

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Insect Science 2020年第5期27卷 908-920页

作者： Qi-Chen Su Xia Wang chan Deng yue-Li yun Yao Zhao yu Peng State Key Laboratory of Biocatalysis and Enzyme Engineering of china School of Life SciencesHubei UniversityWuhanChina

Hybyphantes graminicola is a resident spider species found in maize and cotton fields and is an important biological control agent of various *** studies have demonstrated that stress from elevated CO2 and Wolbachia infection can strongly affect spider ***,based on CO2 levels(400 ppm,current atmospheric CO2 concentration and 800 ppm,high CO2 concentration)and Wolbachia status(Wolbachia-infected,W^+and Wolbachia-uninfected,W^-),we divided *** individuals into four treatment groups:W^-400 ppm,W^-800 ppm,W^+400 ppm,and W^+800 *** investigate the effects of elevated CO2 levels(W^-400 vs W^-800),Wolbachia infection(W^-400 vs W^+400),and the interactions between these two factors(W^-400 Vs W^+800),high-throughput transcriptome sequencing was employed to characterize the de novo transcriptome of the spiders and identify stress-related differentially expressed genes(DEGs).De novo assembly of complementary DNA sequences generated 86688 unigenes,23938 of which were annotated in public databases.A total of 84,21,and 157 DEGs were found among W^-400vs W^-800,W^-400 vs W^+400,and W^-400vs W^+800,*** enrichment analysis revealed that metabolic processes,signaling,and catalytic activity were significantly affected by elevated CO2 levels and Wolbachia *** findings suggest that the impact of elevated CO2 levels and Wolbachia infection on the *** transcriptome was,to a large extent,on genes involved in metabolic *** study is the first description of transcriptome changes in response to elevated CO2 levels and WoIbachia infection in spiders.

关键词： CO2 level high-throughput Hybyphantes graminicola RT-qPCR transcrip-tome Wolbachia infection

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Local charge neutrality condition,Fermi level and majority carrier density of a semiconductor with multiple localized multi-level intrinsic/impurity defects

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Journal of Semiconductors 2011年第11期32卷 1-8页

作者： Ken K.chin Department of Physics and Apollo CdTe Solar Energy Research Center New Jersey Institute of Technology Department of Photonic and Information Engineering School of Electronic and Information EngineeringBeihang University

For semiconductors with localized intrinsic/impurity defects, intentionally doped or unintentionally incorporated, that have multiple transition energy levels among charge states, the general formulation of the local charge neutrality condition is given for the determination of the Fermi level and the majority carrier density. A graphical method is used to illustrate the solution of the problem. Relations among the transition energy levels of the multi-level defect are derived using the graphical method. Numerical examples are given for p-doping of the CdTe thin film used in solar panels and semi-insulating Si to illustrate the relevance and importance of the issues discussed in this work.

关键词： multi-level defects defect complex intrinsic impurity semiconductor Fermi level majority carrier density

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Engineering synthetic optogenetic networks for biomedical applications

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Frontiers of Electrical and Electronic Engineering in china 2017年第2期5卷 111-123页

作者： Meiyan Wang yuanhuan yu Jiawei Shao Boon chin Heng Haifeng Ye Shanghai Key Laboratory of Regulatory Biology Institute of Biomedical Sciences and School of Life Sciences East China Normal University Shanghai 200241 China Department of Endodontology Faculty of Dentistry The University of Hong Kong Hong Kong SAR 999077 China

Background： Recently, optogenetics based on genetically encoded photosensitive proteins has emerged as an innovative technology platform to revolutionize manipulation of cellular behavior through fight stimulation. It has enabled user defined control of various cellular behaviors with spatiotemporal precision and minimal invasiveness, creating unprecedented opportunities for biomedical applications. Results： This article reviews current advances in optogenetic networks designed for the treatment of human diseases. We highlight the advantages of these optogenetic networks, as well as emerging questions and future perspectives. Conclusions： Various optogenetic systems have been engineered to control biological processes at all levels using light and applied for numerous diseases, such as metabolic disorders, cancer, and immune diseases. Continued development of optogenetic modules will be necessary to precisely control of gene expression magnitude towards clinical medical practice in the context of real-world problems.

关键词： synthetic biology mammalian designer cells optogenetics synthetic gene circuits gene- and cell-basedtherapy

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Serum lipid profiles are associated with semen quality

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Asian Journal of Andrology 2017年第6期19卷 633-638页

作者： chin-yu Liu yu-ching Chou Shyh-Hsiang Lin Sheng-Tang Wu Tai-Lung Cha Hong-I Chen Chih-Wei Tsao Department of Nutritional Science Fu Jen Catholic University New Taipei City Taiwan China School of Public Health National Defense Medical Center Taipei Taiwan China School of Nutrition and Health Sciences College of Nutrition Taipei Medical University Taipei Taiwan China Division of Urology Department of Surgery Tri-Service General Hospital National Defense Medical Center Taipei Taiwan China Graduate Institute of Medical Sciences Chang Jung Christian University Tainan Taiwan China

We aimed to explore the associations between different lipid profiles and semen quality in a large-scale general male population. Sperm concentration, total sperm motility, progressive motility, and normal sperm morphology of total 7601 participants were recorded. The association of these semen parameters with the triglyceride, total cholesterol, high-density lipoprotein, low-density lipoprotein, and very low-density lipoprotein of serum lipid profiles was analyzed. Sperm concentration was statistically positively correlated with triglyceride and very low-density lipoprotein （adjusted P= 0.001 and P= 0.005, respectively）. Total sperm motility and progressive motility were statistically increased with increasing low-density lipoprotein and cholesterol levels （both adjusted P = 0.008 and P 〈 0.001, respectively）. The similar J-shaped associations （high-low-low-high） were noted between individual lipid profile and normal sperm morphology, especially low-density lipoprotein and cholesterol with statistical significance （adjusted P = 0.017 and P= 0.021, respectively）. The prevalence of abnormal total sperm motility and progressive motility was decreased in participants with high levels of cholesterol （P = 0.008 and P = 0.019, respectively）, and the reverse J-shaped associations （low-high-high-low） were noted between high-density lipoprotein, triglyceride, very low-density lipoprotein, and the prevalence of abnormal normal sperm morphology （P = 0.010, P = 0.037, and P = 0.025, respectively）. A high cholesterol level was associated with better sperm motility. Similar J-shaped associations were noted between all lipid profiles and normal sperm morphology; meanwhile, the reverse J-shaped trends were identified between them and abnormal normal sperm morphology prevalence.

关键词： cholesterol general population lipoprotein semen quality triglyceride

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Simultaneous determination of manufacturer’s process mean and production run length,and retailer’s order quantity

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Journal of Management Analytics 2016年第1期3卷 59-79页

作者： Chung-Ho Chen Chao-yu Chou Chao-chin Kan Department of Management and Information Technology Southern Taiwan University of Science and TechnologyTainan Department of Finance National Taichung University of Science and TechnologyTaichung

In Chen and Liu’s optimum profit model with a traditional production system,they did not consider the effect of product quality on the customer’s demand order quantity,and also ignored the used cost of customers for *** fact,the customer’s demand quantity is always seriously related to product ***,in the present paper,we modify Chen and Liu’s model to address the determination of the optimal process parameters by employing the idea of quality loss and single sampling rectifying inspection *** that the quality characteristic of the product is normally distributed,Taguchi’s symmetric quadratic quality loss function is applied in evaluating the product *** decision variables,i.e.,the mean of the process characteristic,the production run length of the product and the retailer’s order quantity,are jointly determined in our modified model to maximize the expected total profit of society,which includes both the manufacturer and the retailer.A heuristic solution procedure is developed for this optimization problem,and a numerical example is provided for *** the numerical results,it can be seen that both the sale price per unit and the intercept of the mean demand of the customer are two major(or significant)parameters in the model and should be more accurately estimated in ***,the quality investment policy is provided to compare its effect on the optimum profit model with quality improvement.

关键词： economic order quantity process mean production run length Taguchi’s quadratic quality loss function quality investment

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习近平文化思想科学体系阐释论纲

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新疆师范大学学报（哲学社会科学版） 2025年第1期46卷 16-33,F0002页

作者：范玉刚山东大学文艺美学研究中心山东济南250100 南方科技大学全球城市文明典范研究院广东深圳518055

习近平文化思想是逻辑自恰、相互贯通、结构完整并集时代性、系统性、原创性于一体的开放性科学体系。深刻把握和领会习近平文化思想的核心要义与主导逻辑,需要建构具有有效性的理论阐释框架。文本通过提炼五个重大学术命题,即坚持党对... 详细信息

习近平文化思想是逻辑自恰、相互贯通、结构完整并集时代性、系统性、原创性于一体的开放性科学体系。深刻把握和领会习近平文化思想的核心要义与主导逻辑,需要建构具有有效性的理论阐释框架。文本通过提炼五个重大学术命题,即坚持党对文化工作的全面领导和巩固党的文化领导权,坚定文化自信、建设社会主义文化强国,在不断健全文化体系中增强国家文化软实力,以“两个结合”特别是“第二个结合”引领中华文明发展,在文明互鉴中增强中华文明的传播力影响力,以此为四梁八柱构建阐释习近平文化思想科学体系的理论框架。

关键词：习近平文化思想科学体系文化基本理论重大学术命题理论框架

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Inhibition of the NLRP3 inflammasome attenuates spiral ganglion neuron degeneration in aminoglycoside-induced hearing loss

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Neural Regeneration Research 2025年第10期20卷 3025-3039页

作者： Jia Fang Zhuangzhuang Li Pengjun Wang Xiaoxu Zhang Song Mao Yini Li Dongzhen yu Xiaoyan Li Yazhi Xing Haibo Shi Shankai Yin Department of Otolaryngology Head and Neck Surgery Shanghai Sixth People’s Hospital Affiliated to Shanghai Jiao Tong University School of MedicineOtolaryngology Institute of Shanghai Jiao Tong UniversityShanghai Key Laboratory of Sleep Disordered BreathingShanghaiChina Department of Otorhinolaryngology-Head and Neck Surgery Shanghai Children’s HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghaiChina

Aminoglycosides are a widely used class of antibacterials renowned for their effectiveness and broad antimicrobial ***,their use leads to irreversible hearing damage by causing apoptosis of hair cells as their direct *** addition,the hearing damage caused by aminoglycosides involves damage of spiral ganglion neurons upon *** investigate the mechanisms underlying spiral ganglion neuron degeneration induced by aminoglycosides,we used a C57BL/6J mouse model treated with *** found that the mice exhibited auditory deficits following the acute loss of outer hair *** ganglion neurons displayed hallmarks of pyroptosis and exhibited progressive degeneration over *** profiling of these neurons showed significant upregulation of genes associated with inflammation and immune response,particularly those related to the NLRP3 *** of the canonical pyroptotic pathway in spiral ganglion neurons was observed,accompanied by infiltration of macrophages and the release of proinflammatory *** intervention targeting NLRP3 using Mcc950 and genetic intervention using NLRP3 knockout ameliorated spiral ganglion neuron degeneration in the injury *** findings suggest that NLRP3 inflammasome-mediated pyroptosis plays a role in aminoglycoside-induced spiral ganglion neuron *** of this pathway may offer a potential therapeutic strategy for treating sensorineural hearing loss by reducing spiral ganglion neuron degeneration.

关键词： degeneration hearing loss macrophages Mcc950 neuroinflammation NLRP3 inflammasome ototoxicity pyroptosis sensorineural hearing loss spiral ganglion neuron

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Drosophila models used to simulate human ATP1A1 gene mutations that cause Charcot-Marie-Tooth type 2 disease and refractory seizures

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Neural Regeneration Research 2025年第1期20卷 265-276页

作者： Yao yuan Lingqi yu Xudong Zhuang Dongjing Wen Jin He Jingmei Hong Jiayu Xie Shengan Ling Xiaoyue Du Wenfeng Chen Xinrui Wang Institute of Life Sciences College of Biological Science and EngineeringFuzhou UniversityFuzhouFujian ProvinceChina NHC Key Laboratory of Technical Evaluation of Fertility Regulation for Non-human Primate(Fujian Maternity and Child Health Hospital) FuzhouFujian ProvinceChina Medical Research Center Fujian Maternity and Child Health HospitalCollege of Clinical Medicine for Obstetrics&Gynecology and PediatricsFujian Medical UniversityFuzhouFujian ProvinceChina Department of Neurology and Institute of Neurology of The First Affiliated Hospital Institute of Neuroscienceand Fujian Key Laboratory of Molecular NeurologyFujian Medical UniversityFuzhouFujian ProvinceChina

Certain amino acids changes in the human Na^(+)/K^(+)-ATPase pump,ATPase Na^(+)/K^(+)transporting subunit alpha 1(ATP1A1),cause Charcot-Marie-Tooth disease type 2(CMT2)disease and refractory *** develop in vivo models to study the role of Na^(+)/K^(+)-ATPase in these diseases,we modified the Drosophila gene homolog,Atpα,to mimic the human ATP1A1 gene mutations that cause *** located within the helical linker region of human ATP1A1(I592T,A597T,P600T,and D601F)were simultaneously introduced into endogenous Drosophila Atpαby CRISPR/Cas9-mediated genome editing,generating the Atpα^(TTTF)*** addition,the same strategy was used to generate the corresponding single point mutations in flies(Atpα^(I571T),Atpα^(A576T),Atpα^(P579T),and Atpα^(D580F)).Moreover,a deletion mutation(Atpα^(mut))that causes premature termination of translation was generated as a positive *** these alleles,we found two that could be maintained as homozygotes(Atpα^(I571T)and Atpα^(P579T)).Three alleles(Atpα^(A576T),Atpα^(P579)and Atpα^(D580F))can form heterozygotes with the Atpαmut *** found that the Atpαallele carrying these CMT2-associated mutations showed differential phenotypes in *** heterozygous for Atpα^(TTTF)mutations have motor performance defects,a reduced lifespan,seizures,and an abnormal neuronal *** Drosophila models will provide a new platform for studying the function and regulation of the sodium-potassium pump.

关键词： ATP1A1 Atpα bang-sensitive paralysis Charcot-Marie-Tooth disease type 2 CRISPR/Cas9 homology-directed repair Na^(+)/K^(+)-ATPase point mutation seizures sodium pump

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Understanding the beam self-cleaning behavior of ultrashort laser pulse filamentation

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Science china(Technological Sciences) 2007年第4期50卷 413-421页

作者： See Leang chin Department of Physics Engineering Physics and Optics & Center for Optics photonics and Laser (COPL) Laval University Quebec Canada G1K 7P4

In this paper we report a recent study on the beam self-cleaning behavior occurred during the ultrashort laser pulse filamentation process. The propagation of a Gaussian beam with distorted beam profile is numerically simulated based on the nonlinear wave equation. Our results demonstrate that when the power is not too high so that multiple filaments are not yet induced, the intensity perturbation con-tained in the initial beam profile could be treated as high order spatial modes su-perpositioning on a fundamental mode. Then the self-focusing of the laser beam acts as a spatial filter. It focuses the fundamental mode toward the propagation axis, and produces a fundamental mode profile at the self-focus. While the strong diffraction of higher order modes could not be counteracted by the self-focusing. Therefore their propagation is mainly governed by the divergence without de-stroying the high profile quality at the self-focal region. These lead to the observa-tion of beam profile self-cleaning behavior.

关键词： filamentation ultrashort pulse self-cleaning

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Hypoxia-preconditioned bone marrow-derived mesenchymal stem cells protect neurons from cardiac arrest-induced pyroptosis

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Neural Regeneration Research 2025年第4期20卷 1103-1123页

作者： Xiahong Tang Nan Zheng Qingming Lin Yan You Zheng Gong Yangping Zhuang Jiali Wu yu Wang Hanlin Huang Jun Ke Feng Chen Shengli Clinical Medical College of Fujian Medical University Fujian Medical UniversityFuzhouFujian ProvinceChina Department of Emergency Fujian Provincial HospitalFuzhouFujian ProvinceChina Fujian Provincial Key Laboratory of Emergency Medicine FuzhouFujian ProvinceChina The Second Department of Intensive Care Unit Fujian Provincial Hospital South BranchFuzhouFujian ProvinceChina

Cardiac arrest can lead to severe neurological impairment as a result of inflammation,mitochondrial dysfunction,and post-cardiopulmonary resuscitation neurological *** preconditioning has been shown to improve migration and survival of bone marrow–derived mesenchymal stem cells and reduce pyroptosis after cardiac arrest,but the specific mechanisms by which hypoxia-preconditioned bone marrow–derived mesenchymal stem cells protect against brain injury after cardiac arrest are *** this end,we established an in vitro co-culture model of bone marrow–derived mesenchymal stem cells and oxygen–glucose deprived primary neurons and found that hypoxic preconditioning enhanced the protective effect of bone marrow stromal stem cells against neuronal pyroptosis,possibly through inhibition of the MAPK and nuclear factor κB ***,we transplanted hypoxia-preconditioned bone marrow–derived mesenchymal stem cells into the lateral ventricle after the return of spontaneous circulation in an 8-minute cardiac arrest rat model induced by *** results showed that hypoxia-preconditioned bone marrow–derived mesenchymal stem cells significantly reduced cardiac arrest–induced neuronal pyroptosis,oxidative stress,and mitochondrial damage,whereas knockdown of the liver isoform of phosphofructokinase in bone marrow–derived mesenchymal stem cells inhibited these *** conclude,hypoxia-preconditioned bone marrow–derived mesenchymal stem cells offer a promising therapeutic approach for neuronal injury following cardiac arrest,and their beneficial effects are potentially associated with increased expression of the liver isoform of phosphofructokinase following hypoxic preconditioning.

关键词： bone marrow–derived mesenchymal stem cells cardiac arrest cardiac resuscitation hypoxic preconditioning liver isoform of phosphofructokinase mitochondria neuroinflammation oxidative stress pyroptosis reactive oxygen species

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