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Antihepatocarcinoma Effect of Solanine and Its Mechanisms

Chinese Herbal Medicines 2012年第2期4卷 126-135页

作者： yu-bin ji[a][b] Shi-yong GAO[a][b] Center for Life Sciences and Environmental Sciences Harbin University of Commerce Harbin 150076 China MOE Engineering Research Center of Natural Anticancer Drugs Harbin University of Commerce Harbin 150076 China Center for Life Sciences and Environmental Sciences Harbin University of Commerce Harbin 150076 China MOE Engineering Research Center of Natural Anticancer Drugs Harbin University of Commerce Harbin 150076 China

Objective To explore the antitumor effect of solanine and its *** The in vivo antitumor effect of solanine was observed using models developed through in vivo transplantation of tumor cells;In vitro lines of sensitive antitumor cells were selected from the digestive system using MTT assay;The effect of solanine on cell morphology was observed using transmission electronic microscopy;The morphology of apoptotic cells was observed using Annexin V/PI double staining and laser confocal scanning microscopy(LCSM);The rate of cell apoptosis was measured using Annexin V/PI double staining and flow cytometry;The concentration of intracellular Ca 2+ ([Ca 2+ ]i)was determined using Fluo-3/AM staining and LCSM;The membrane potential of cellular mitochondria was determined using TMRE staining and LCSM;The protein expression of Bcl-2 and Bax was measured using immunological marking and LCSM;And the activity of caspase-3 was measured using the colorimetric *** Solanine could inhibit the growth of tumor weight in S180 tumor-bearing mice and prolong the survival time of H22 tumor-bearing *** assay revealed that HepG2 cells were quite sensitive to solanine because solanine could induce morphological changes in HepG2 cells,with the rate of early apoptosis being 4%,8.5%,and 20.1%,for HepG2 cells treated for 24 h with solanine at concentration of 0.4,2,and 10μg/mL, *** could raise the[Ca 2+ ]i and lower the membrane *** could reduce the protein expression of Bcl-2 while increase that of Bax,thus increasing the activity of *** The obvious antitumor activity of solanine in human hepatocarcinoma is *** inhibitory effect is achieved through solanine decreasing the Bcl-2/Bax ratio,thus increasing[Ca 2+ ]i,which could enhance the enzymatic activity of the caspase family,thus inducing the apoptosis of HepG2 cells.

关键词： apoptosis caspase hepatocarcinoma S180 solanine

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Effect of solanine on the membrane potential of mitochondria in HepG_2 cells and [Ca^(2+)]i in the cells

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World Journal of Gastroenterology 2006年第21期12卷 3359-3367页

作者： Shi-Yong Gao Qiu-Juan Wang yu-bin ji Physiology Section Department of Pharmacy China Pharmaceutical University Nanjing 210009 Jiangsu Province China Postdoctoral Research Station The Institute of Materia Medica Harbin University of Commerce Harbin 150076 Heilongjiang Province China

AIM： To observe the effect of solanine on the membrane potential of mitochondria in HepG2 cells and [Ca^2＋]i in the cells, and to uncover the mechanism by which solanine induces ***： HepG2 cells were double stained with AO/EB, and morphological changes of the cells were observed using laser confocal scanning microscopy （LCSM）. HepG2 cells were stained with TMRE, and change in the membrane potential of mitochondria in the cells were observed using LCSM. HepG2 cells were double stained with Fluo-3/AM, and change of [Ca^2＋]i in the cells were observed using LCSM. HepG2 cells were double stained with TMRE and Fluo-3/AM, and both the change in membrane potential of mitochondria and that of [Ca^2＋]i in the cells were observed using ***： Cells in treated groups showed typical signs of apoptosis. Staining with TMRE showed that solanine could lower membrane potential; staining with Fluo-3/AM showed that solanine could increase the concentration of Ca^2＋ in tumor cells; and those of double staining with TMRE and Fluo-3/AM showed that solanine could increase the concentration of Ca^2＋ in the cells at the same time as it lowered the membrane potential of ***： Solanine opens up the PT channels in the membrane by lowering the membrane potential, leading to Ca^2＋ being transported down its concentration gradient, which in turn leads to the rise of the concentration of Ca^2＋ in the cell, turning on the mechanism for apoptosis.

关键词： Solanine Hepatocarcinomatic cell Ca^2+ in the cell Membrane potential Laser confocal scanning microscopy

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Arylamine N-acetyltransferases: a new inhibitor of apoptosis in HepG2 cells

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浙江中西医结合杂志 2008年第9期18卷 701-706页

作者： yu-bin ji Shi-yong GAO Postdoctoral Research Station of the Institute of Materia Medica Center for Life Sciences and Environmental Sciences Harbin University of Commerce Harbin 150076 China MOE Engineering Research Center of Natural Anticancer Drugs Harbin University of Commerce Harbin 150076 China

Objective: To explore how arylamine N-acetyltransferases (NATs) is related to cell apoptosis. Methods: NAT activity in apoptotic HepG2 cells was measured using high performance liquid chromatography (HPLC); the apoptosis rate of HepG2 cells acted upon by an NAT inhibitor was measured using flow cytometry. Results: NAT activity was lowered in apoptotic HepG2 cells; apoptosis rate induced by camptothecin (CAM) increased after inhibition of NAT activity in HepG2 cells. Conclusion: NAT can inhibit apoptosis in HepG2 cells.

关键词： HepG2细胞细胞凋亡抑制剂生物活性

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Study on HepG-2 apoptosis induced by saponins isolated from Asparagus and the effects on the activities of caspase-3,8,9

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沈阳药科大学学报 2008年第S1期25卷 66-67页

作者： ji yu-bin,XU He,ji Chen-feng(Center of Research on Life Sciences and Environmental Sciences,Harbin University of Commerce,Harbin 150076,China Engineering Research Center of Natural Anticancer Drugs,Ministry of Education,Harbin 150076,China)

Objective To study the effect of saponins of asparagus on apoptosis and the variations of caspase8,caspase-9 and caspase-3 activity in the process of asparagus induced apoptosis in HepG-2,to investigate the apoptosis mechanism *** Asparagus on apoptosis effects on tumor cells cultured-HepG-2 with different concentrations at different time,IC50 value was measured by MTT assay,the apoptosis rate was determined by FCM with AnnexinV/PI staining,their apoptotic morphology were observed by electron microscopy and Colorimetric method was used to measure caspase-8,9 and caspase-3 *** Experiments of antitumour in vivo showed that saponins of asparagus can inhibit the growth of tumor cell of HepG-2 in evidence,IC50 was 101.15 mg·*** for 72 h,the apoptosis rate had positive increased with *** morphology was observed by electron *** activities of caspase-8,caspase-9 and caspase-3 had positive increased with *** have significant difference compared with negative control group(P<0.01).The activities of caspase-8 were high at 24 h,but the activities of caspase-9 and caspase-3 is high at 48 *** Aaponins of asparagus can inhibit the growth of tumor cell of HepG2,and the underlying mechanism might be related to up regulation of caspase-8,9 activity which subsequently transforms caspase-3 into its active form.

关键词： saponins asparagus HepG-2 apoptosis mechanism

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Arylamine N-acetyltransferases: a new inhibitor of apoptosis in HepG2 cells

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2008年第9期9卷 701-706页

作者： yu-bin ji1,2, Shi-yong GAO1,2 (1Postdoctoral Research Station of the Institute of Materia Medica, Center for Life Sciences and Environmental Sciences, Harbin University of Commerce, Harbin 150076, China) (2MOE Engineering Research Center of Natural Anticancer Drugs, Harbin University of Commerce, Harbin 150076, China) Postdoctoral Research Station of the Institute of Materia Medica Center for Life Sciences and Environmental Sciences Harbin University of Commerce Harbin 150076 China MOE Engineering Research Center of Natural Anticancer Drugs Harbin University

关键词： Arylamine N-acetyltransferases (NATs) Apoptosis HepG2 Camptothecin (CAM)

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含氟树脂对细旦涤纶染色的增深作用

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印染 2007年第6期33卷 5-7页

作者：俞季斌谢孔良东华大学现代纺织研究院上海200051

以丙烯酸丁酯和甲基丙烯酸六氟丁酯为单体,采用乳液聚合法合成含氟树脂,用于提高涤纶染色织物的表观深度。改变各单体的配比制备一系列乳液,对其增深效果进行比较,并研究了含氟聚合物结构、树脂用量等条件对增深效果的影响。结果表明,... 详细信息

以丙烯酸丁酯和甲基丙烯酸六氟丁酯为单体,采用乳液聚合法合成含氟树脂,用于提高涤纶染色织物的表观深度。改变各单体的配比制备一系列乳液,对其增深效果进行比较,并研究了含氟聚合物结构、树脂用量等条件对增深效果的影响。结果表明,使用含氟单体对丙烯酸树脂进行改性,具有一定的增深效果,可应用于细旦涤纶织物染色后的增深。

关键词：染色增深效果氟聚合物丙烯酸酯织物聚对苯二甲酸乙二酯纤维

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分层阻抗理论在永磁电机转子参数分析中应用

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微电机 1999年第5期32卷 50-52页

作者：谷爱昱邹继斌哈尔滨工业大学黑龙江哈尔滨150001

分层阻抗理论是研究实心转子感应电机的一种有效方法，表面磁钢永磁同步电机转子结构与复合转子电机具有相似性。

关键词：永磁电机转子参数分析分层阻抗理论

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The effect of Sachalin rhodiola rhizome extract on liver starch and liver cell morphous of sports fatigue rat

The effect of Sachalin rhodiola rhizome extract on liver sta...

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两岸三地药理学与临床药理学术会议

作者： ji yu-bin LI Rui ji Chen-feng (Center of Research on Life Sciences and Environmental Sciences, Harbin University of Commerce, Harbin 150076, China Engineering Research Center of Natural Anticancer Drugs, Ministry of Education, Harbin 150076, China)

Objective To inspect the effect of Sachalin rhodiola rhizome extract to the swimming time and content of liver starch and liver cells morphous of sports fatigue *** Using weight loading swim- ming to determine swimming time,using kits to determine liver starch,using transmission electron micro- scope to observe the diversify of rat liver cells morphous and *** To compare with negative control group,the Sachalin rhodiola rhizome extract can obviously extend survival time of swimming rat,in- crease the content of liver *** Sachalin rhodiola rhizome extract can raise the staying power of sports fatigue rat,strengthen sport ability and play a part in antifatigue by increasing the content of liver starch and protecting liver cells of sports fatigue rat.

关键词： sachalin rhodiola rhizome extract sports fatigue transmission electron microscope

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Study on HepG-2 apoptosis induced by saponins isolated from Asparagus and the effects on the activities of caspase-3,8,9

Study on HepG-2 apoptosis induced by saponins isolated from ...

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两岸三地药理学与临床药理学术会议

作者： ji yu-bin XU He ji Chen-feng (Center of Research on Life Sciences and Environmental Sciences, Harbin University of Commerce, Harbin 150076, China Engineering Research Center of Natural Anticancer Drugs, Ministry of Education, Harbin 150076, China)

Objective To study the effect of saponins of asparagus on apoptosis and the variations of caspase8, caspase-9 and caspase-3 activity in the process of asparagus induced apoptosis in HepG-2,to investigate the apoptosis mechanism *** Asparagus on apoptosis effects on tumor cells cultured-HepG-2 with different concentrations at different time,ICvalue was measured by MTT assay,the apoptosis rate was de- termined by FCM with AnnexinV/PI staining,their apoptotic morphology were observed by electron mi- croscopy and Colorimetric method was used to measure caspase-8,9 and caspase-3 *** Experi- ments of antitumour in vivo showed that saponins of asparagus can inhibit the growth of tumor cell of HepG-2 in evidence,ICwas 101.15 mg·*** for 72 h,the apoptosis rate had positive increased with *** morphology was observed by electron *** activities of caspase- 8,caspase-9 and caspase-3 had positive increased with *** have significant difference com- pared with negative control group(P<0.01).The activities of caspase-8 were high at 24 h,but the activi- ties of caspase-9 and caspase-3 is high at 48 *** Aaponins of asparagus can inhibit the growth of tumor cell of HepG2,and the underlying mechanism might be related to up regulation of caspase-8,9 activi- ty which subsequently transforms caspase-3 into its active form.

关键词： saponins asparagus HepG-2 apoptosis mechanism

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漆酶在饺子皮面团制作中的应用

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食品工业科技 2020年第8期41卷 62-66,73页

作者：闵照永路源汪雅馨于吉斌王雯斐师玉忠鹤壁职业技术学院河南鹤壁458030 河南科技学院新科学院河南新乡453003 漯河食品职业学院河南漯河462000 新乡富元食品有限公司河南新乡453000 河南科技学院食品学院粮食资源深度利用河南省工程实验室河南新乡453003

为了探究漆酶对饺子皮品质的影响,分别向面粉中添加0、0.05%、0.1%、0.15%的漆酶(均以面粉质量计)观测漆酶对饺子皮品质的改善状况,并利用质构仪和色差计对饺子皮面团以及饺子皮相关特性进行测定。结果表明:随着漆酶添加量的增加,饺子... 详细信息

为了探究漆酶对饺子皮品质的影响,分别向面粉中添加0、0.05%、0.1%、0.15%的漆酶(均以面粉质量计)观测漆酶对饺子皮品质的改善状况,并利用质构仪和色差计对饺子皮面团以及饺子皮相关特性进行测定。结果表明:随着漆酶添加量的增加,饺子皮的总色度值L、黄度值a、综合感官评分呈现增加趋势,且添加漆酶量为0.15%时饺子皮感官评分较高。品质雷达图结果表明漆酶的添加量对饺子皮筋性、可塑性、汤特征等指标有显著的改善作用。饺子皮面团质构测定结果表明饺子皮面团的硬度、弹性、咀嚼性与漆酶的添加量成正相关,相关系数分别为0.986、0.996、0.826,对饺子皮面团的回复性影响不显著;相应地,随着漆酶量的增加,面粉中游离巯基含量呈显著减小趋势,面粉持水率呈现先增后减的趋势,且在添加量为0.1%时,面粉的持水率最高,饺子皮的烹煮损失呈递减趋势,且在漆酶添加量为0.15%烹煮损失最低,此情况下制成的饺子皮的口感和品质较好。

关键词：漆酶质构色差持水力饺子皮面团

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