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Folate levels in mucosal tissue but not methylenetetrahydrofolate reductase polymorphisms are associated with gastric carcinogenesis

引用

World Journal of Gastroenterology 2006年第47期12卷 7591-7597页

作者： yu-rong weng dan-feng sun jing-yuan fang wei-qi gu hong-yin zhu Shanghai Institute of Digestive Disease ShanghaiJiao-Tong University School of Medicine Renji HospitalShanghai 200001 China

AIM: To evaluate whether folate levels in mucosal tissue and some common methylenetetrahydrofolate reductase (MTHFR) variants are associated with the risk of gastric cancer through DNA methylation. METHODS: Real-time PCR was used to study the expression of tumor related genes in 76 mucosal tissue samples from 38 patients with gastric cancer. Samples from the gastroscopic biopsy tissues of 34 patients with chronic superficial gastritis (CSG) were used as controls. Folate concentrations in these tissues were detected by the FOL ACS: 180 automated chemiluminescence system. MTHFR polymorphisms were analyzed by PCR-RFLP, and the promoter methylation of tumor-related genes was determined by methylation-specific PCR (MSP). RESULTS: Folate concentrations were significantly higher in CSG than in cancerous tissues. Decreased expression and methylation of c-myc accompanied higher folate concentrations. Promoter hypermethylation and loss of p16INK4A in samples with MTHFR 677CC were more frequent than in samples with the 677TT or 677CT genotype. And the promoter hypermethylation and loss of p21WAF1 in samples with MTHFR 677CT were more frequent than when 677CC or 677TT was present. The 677CT genotype showed a non-significant higher risk for gastric cancer as compared with the 677CC genotype. CONCLUSION: Lower folate levels in gastric mucosal tissue may confer a higher risk of gastric carcinogenesisthrough hypomethylation and overexpression of c-myc.

关键词： Folate Methylenetetrahydrofolate reductase Polymorphism DNA methylation Gastric cancer

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引用

世界华人消化杂志 2006年第22期14卷 2192-2198页

作者：翁玉蓉房静远孙丹凤陈朝飞陆嵘顾伟齐朱红音上海交通大学医学院附属仁济医院上海市消化疾病研究所上海市200001

目的:探讨人胃癌组织中癌基c-myc,抑癌基因p16INK4A,p21WAF1,错配修复基NhMLH1和hM2SH2的甲基化状态及其表达与叶酸、MTHFR基因多态性的关系．方法:胃癌38例手术切除标本的癌区、癌旁和外周正常黏膜组织,运用FOL ACS:180自动化学发光系... 详细信息

目的:探讨人胃癌组织中癌基c-myc,抑癌基因p16INK4A,p21WAF1,错配修复基NhMLH1和hM2SH2的甲基化状态及其表达与叶酸、MTHFR基因多态性的关系．方法:胃癌38例手术切除标本的癌区、癌旁和外周正常黏膜组织,运用FOL ACS:180自动化学发光系统测定叶酸含量,PCR-RFLP技术检测MTHFR基因677(C→T)和1298(A→C)两个常见多态,并分别以Real—time RT-PCR和甲基化特异性PCR (MSP)技术检测肿瘤相关基因的表达和甲基化状态．结果:c-myc表达升高,p16INK4A,hMLH1和hMSH2表达降低的胃癌黏膜组织其基因启动子区异常甲基化．p21WAF1,hMSH2表达降低, p16INK4A高甲基化者叶酸水平明显降低,c-myc低甲基化和表达升高者中均存在低叶酸水平．MTHFR 677CC基因型的胃癌黏膜组织p16INK4A甲基化升高且表达降低,而其余肿瘤相关基因的甲基化及其表达与MTHFR两个常见多态均无明显相关性．结论:DNA甲基化在胃癌的发生、发展中具有重要作用,叶酸水平和MTHFR基因多态性通过影响部分肿瘤相关基因的甲基化状态而调控其表达．

关键词：胃癌肿瘤相关基因 DNA甲基化叶酸 MTHFR 基因多态性

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Timing analysis of the black hole candidate EXO 1846–031 with Insight-HXMT monitoring

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Research in Astronomy and Astrophysics 2021年第3期21卷 193-204页

作者： He-Xin Liu yue Huang guang-Cheng Xiao qing-Cui Bu Jin-Lu Qu Shu Zhang Shuang-Nan Zhang Shu-Mei Jia fang-Jun Lu Xiang Ma Lian Tao wei Zhang Li Chen Li-Ming Song Ti-Pei Li yu-Peng Xu Xue-Lei Cao Yong Chen Cong-Zhan Liu Ce Cai Zhi Chang Gang Chen Tian-Xiang Chen Yi-Bao Chen yu-Peng Chen wei Cui wei-wei Cui jing-Kang Deng Yong-wei Dong yuan-yuan Du Min-Xue Fu guan-Hua Gao He Gao Min Gao Ming-yu Ge yu-Dong gu Ju guan Cheng-Cheng guo Da-wei Han Jia Huo Lu-Hua Jiang wei-Chun Jiang jing Jin Yong-Jie Jin Ling-Da Kong Bing Li Cheng-Kui Li Gang Li Mao-Shun Li wei Li Xian Li Xiao-Bo Li Xu-fang Li Yang-guo Li Zheng-wei Li Xiao-Hua Liang jing-yuan Liao Bai-Sheng Liu guo-qing Liu hong-wei Liu Xiao-jing Liu Yi-Nong Liu Bo Lu Xue-feng Lu qi Luo Tao Luo Bin Meng Yi Nang Jian-yin Nie Ge Ou Na Sai Ren-Cheng Shang Xin-ying Song Liang sun ying Tan You-Li Tuo Chen Wang guo-feng Wang Juan Wang Ling-Jun Wang weng-Shuai Wang yu-Sa Wang Xiang-Yang Wen Bai-Yang Wu Bo-Bing Wu Mei Wu Shuo Xiao Shao-Lin Xiong He Xu Jia-wei Yang Sheng Yang Yan-Ji Yang Yi-Jung Yang qi-Bin Yi qian-qing yin yuan You Ai-Mei Zhang Cheng-Mo Zhang Fan Zhang hong-Mei Zhang Juan Zhang Tong Zhang Wan-Chang Zhang Wen-Zhao Zhang Yi Zhang Yi-Fei Zhang Yong-Jie Zhang yuan-Hang Zhang yue Zhang Zhao Zhang Zhi Zhang Zi-Liang Zhang Hai-Sheng Zhao Xiao-Fan Zhao Shi-Jie Zheng Yao-guang Zheng Deng-Ke Zhou Jian-feng Zhou yu-Xuan zhu Ren-Lin zhuang yue zhu Key Laboratory of Particle Astrophysics Institute of High Energy PhysicsChinese Academy of SciencesBeijing 100049China University of Chinese Academy of Sciences Chinese Academy of SciencesBeijing 100049China Institut fur Astronomie und Astrophysik Kepler Center for Astro and Particle PhysicsEberhard Karls Universität72076 TubingenGermany Department of Astronomy Beijing Normal UniversityBeijing 100088China Department of Astronomy Tsinghua UniversityBeijing 100084China Department of Physics Tsinghua UniversityBeijing 100084China Department of Engineering Physics Tsinghua UniversityBeijing 100084China Key Laboratory of Space Astronomy and Technology National Astronomical ObservatoriesChinese Academy of SciencesBeijing 100101China College of Physics Sciences&Technology Hebei UniversityBaoding 071002Hebei ProvinceChina

We present the observational results from a detailed timing analysis of the black hole candidate EXO 1846-031 during its outburst in 2019 with the observations of Insight-HXMT,NICER and *** outburst can be classified roughly into four different ***-C quasi-periodic oscillations(QPOs)observed by NICER(about 0.1-6 Hz)and Insight-HXMT(about 0.7-8 Hz)are also reported in this ***,we study various physical quantities related to QPO *** QPO rms-frequency relationship in the energy band 1-10 keV indicates that there is a turning pointing in frequency around2 Hz,which is similar to that of GRS 1915+105.A possible hypothesis for the relationship above may be related to the inclination of the source,which may require a high inclination to explain *** relationships between QPO frequency and QPO rms,hardness,total fractional rms and count rate have also been found in other transient sources,which can indicate that the origin of type-C QPOs is non-thermal.

关键词： X-ray binaries black hole candidate QPO EXO 1846–031

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mTOR信号通路与组蛋白乙酰化在胃癌细胞中的相互作用

引用

中华医学遗传学杂志 2007年第4期24卷 387-391页

作者：孙丹凤房静远张燕捷田筱青朱红音李恩灵顾伟奇沈冠凤上海交通大学医学院附属仁济医院上海市消化疾病研究所 200001

目的研究哺乳动物雷帕霉素靶蛋白（mammalian target of mpamycin，mTOR）信号通路与组蛋白乙酰化在人胃癌细胞生存活力、细胞周期、相关基因及蛋白表达方面的相互作用。方法分别单独或联合脱乙酰化酶抑制剂曲古抑菌素（trichostatinA，... 详细信息

目的研究哺乳动物雷帕霉素靶蛋白（mammalian target of mpamycin，mTOR）信号通路与组蛋白乙酰化在人胃癌细胞生存活力、细胞周期、相关基因及蛋白表达方面的相互作用。方法分别单独或联合脱乙酰化酶抑制剂曲古抑菌素（trichostatinA，TSA）、mTOR抑制剂雷帕霉素和P13K抑制剂LY294002干预人胃癌MKN45和SGC7901细胞，以噻唑蓝法检测细胞生存活力，流式细胞术检测细胞周期，实时定量PCR检测p21咖基因表达情况，蛋白免疫印迹检测Akt、pTOS6K及4E-BPI蛋白表达情况。结果联合用药比单独用药对胃癌细胞生长抑制作用更明显（P〈0．01）；不管单独或联合用药，均可使MKN45细胞阻滞在G2期（P〈0．05），使SGC7901细胞阻滞在Gl或G2期（P〈0．05）；联合用药比单独用药更能提高抑癌基因p21^WAF1的表达；联合用药使Akt、pTOS6K及4E-BPI磷酸化表达较单独用药显著下降（P〈0．01），联合用药后组蛋白H4／H3乙酰化也较单独用药表达升高（P〈0．01）。结论mTOR信号通路与组蛋白乙酰化在胃癌的发生发展中存在相互关系，mTOR抑制剂与脱乙酰化酶抑制剂联合应用具有协同作用，可望成为肿瘤基因防治的新靶点。

关键词：雷帕霉素靶蛋白雷帕霉素组蛋白乙酰化胃癌

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