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In Vitro Assessment of Irritation Potential of an Anti-Melanogenic Agent,PF3758309,in a Reconstructed Human Epidermis Model

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北京日化 2018年第4期 33-40页

作者： Ayeong lee yu rim lee Ji Yea Kim Jin Hyuk Jung Young Kum Park yu-Jung Kim Sung-Nae lee Sungkwan An Seunghee Bae Research Institute for Molecular-Targeted Drugs Department of Cosmetics EngineeringKonkuk UniversitySeoulKorea Korea Institute of Dermatological Sciences SeoulKorea Gragem Corporation Cheongju-siChuncheongbuk-doKorea Department of Beauty Yakson Care Yeoju Institute of TechnologyYeoju-siGyeongi-doKorea Department of Cosmetology Kyung-In Women's UniversityIncheonKorea

Purpose:This study was designed to determine whether a novel anti-melanogenic agent,PF3758309,has the potential to cause acute cutaneous irritation using a human skin equivalent model (HSEM).Methods:Human skin equivalent was constructed through incubation of normal human derived epidermal keratinocytes (HEKs)on collagen matrix insert with proliferation *** constructed human skin equivalent,the irritation potential of PF3758309 were investigated whether the viability of this agent-treated HESM is under 50%(irritant)or not (non-irritant)using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT)assay after applying the agent to the epidermal surface of the ***,the PF3758309-mediated anti-pigmentation effects were analyzed using Fontana-Masson staining in the ***:The integrity of the constructed HSEM was confirmed using immunohistochemical staining with differentiation markers of epidermis,and observed that Keratin 5,Keratin I and Involucrin were stained in basal,supra-basal and granular layers,*** vitro irritation assay showed that the mean viabilities of the PF3758309 was 83.6%,78.8%and 77.8%at the treatment doses of 0.2,0.5 and I mg,respectively;however,the mean viability of the positive control (5%sodium dodecyl sulfate)-treated HESM was 2.8%.Also,in vitro corrosion assay showed that the mean viabilities of the PF3758309 was 95.3%,95.0%and 94.2% at the treatment doses of 0.2,0.5 and 1 mg,***,using a Fontana-Masson staining assay,the melanin levels in the PF3758309-treated HSEM was significantly decreased compared with the levels in control ***: The anti-melanogenic agent,PF3758309,has no skin irritation potential under the conditions used in this study.

关键词： Human skin equivalent PF3758309 Irritation,Skin Anti-melanogenesis

Positive regulation of cell proliferation by the miR-1290-EHHADH axis in hepatocellular carcinoma

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Cancer Communications 2024年第6期44卷 705-709页

作者： Jinkwon lee Gyeonghwa Kim Tae-Su Han Eunsun Jung Taesang Son Kwangho Kim Kiyoon Kwon yuna Roh Tae Young Ryu In Hwan Tae yunsang Kang Byungheon lee yu rim lee Soo Young Park Won Young Tak Dae-Soo Kim Mi-Young Son Keun Hur Hyun-Soo Cho Stem Cell Convergence Research Center Korea Research Institute of Bioscience and BiotechnologyDaejeonRepublic of Korea Department of Bioscience Korea University of Science and TechnologyDaejeonRepublic of Korea Department of Biochemistry and Cell Biology School of MedicineKyungpook National UniversityDaeguRepublic of Korea Department of Biological Science Sungkyunkwan UniversitySuwonRepublic of Korea Department of Internal Medicine School of MedicineKyungpook National University HospitalDaeguRepublic of Korea Department of Biomedical Convergence Science and Technology School of ConvergenceKyungpook National UniversityDaeguRepublic of Korea

Hepatocellular carcinoma(HCC)is the second most common cancer and the third leading cause of cancer-related death worldwide[1].Recently,HCC incidence and mortality rates have further increased due to changes in the global social environment and dietary habits[2].HCC is treated by surgical resection or combination chemotherapy,but the overall survival rate of HCC patients has not improved,and the recurrence rate is still high due to strong invasiveness and resistance to chemotherapy[3].

关键词： chemotherapy mortality cancer

In Vitro Assessment of Irritation Potential of an Anti-Melanogenic Agent, PF3758309, in a Reconstructed Human Epidermis Model

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In Vitro Assessment of Irritation Potential of an Anti-Melan...

Pan-TRK positive uterine sarcoma in immunohistochemistry without neurotrophic tyrosine receptor kinase gene fusions:A case report

Purpose: This study was designed to determine whether a novel anti-melanogenic agent, PF3758309, has the potential to cause acute cutaneous irritation using a human skin equivalent model(HSEM). Methods: Human skin equivalent was constructed through incubation of normal human derived epidermal keratinocytes(HEKs) on collagen matrix insert with proliferation media. Using constructed human skin equivalent, the irritation potential of PF3758309 were investigated whether the viability of this agent-treated HESM is under 50%(irritant) or not(non-irritant) using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT) assay after applying the agent to the epidermal surface of the HSEM. Also, the PF3758309-mediated anti-pigmentation effects were analyzed using Fontana-Masson staining in the HSEM. Results: The integrity of the constructed HSEM was confirmed using immunohistochemical staining with differentiation markers of epidermis, and observed that Keratin 5, Keratin 1 and Involucrin were stained in basal, supra-basal and granular layers, respectively. In vitro irritation assay showed that the mean viabilities of the PF3758309 was 83.6%, 78.8% and 77.8% at the treatment doses of 0.2, 0.5 and 1 mg, respectively;however, the mean viability of the positive control(5% sodium dodecyl sulfate)-treated HESM was 2.8%. Also, in vitro corrosion assay showed that the mean viabilities of the PF3758309 was 95.3%, 95.0% and 94.2% at the treatment doses of 0.2, 0.5 and 1 mg, respectively. Furthermore, using a Fontana-Masson staining assay, the melanin levels in the PF3758309-treated HSEM was significantly decreased compared with the levels in control HSEM. Conclusion: The anti-melanogenic agent, PF3758309, has no skin irritation potential under the conditions used in this study.

关键词： Human skin equivalent PF3758309 Irritation Skin Anti-melanogenesis

来源： cnki会议评论

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World Journal of Clinical Cases 2025年第2期13卷 39-49页

作者： Seungmee lee yu-Ra Jeon Changmin Shin Sun-Young Kwon Sojin Shin Department of Obstetrics and Gynecology Keimyung University School of MedicineDaegu 42601South Korea Department of Pathology and Institute for Cancer Research Keimyung University School of MedicineDaegu 42601South Korea

BACKGROUND The classification of uterine sarcomas is based on distinctive morphological and immunophenotypic characteristics,increasingly supported by molecular genetic *** on neurotrophic tyrosine receptor kinase(NTRK)gene fusionpositive uterine sarcoma,potentially aggressive and morphologically similar to fibrosarcoma,are limited due to its recent ***-TRK immunohistochemistry(IHC)analysis serves as an effective screening tool with high sensitivity and specificity for NTRK-fusion *** SUMMARY We report a case of a malignant mesenchymal tumor originating from the uterine cervix,which was pan-TRK IHC-positive but lacked NTRK gene fusions,accompanied by a brief literature review.A 55-year-old woman presented to the emergency department with abdominal pain and distension,exhibiting significant ascites and multiple solid pelvic *** examination revealed a tumor encompassing the uterine cervix,extending to the vagina and uterine corpus.A punch biopsy of the cervix indicated NTRK sarcoma with positive immunochemical pan-TRK ***,subsequent next generation sequencing revealed no NTRK gene fusion,leading to a diagnosis of poorly differentiated,advanced-stage *** The clinical significance of NTRK gene fusion lies in potential treatment with TRK inhibitors for positive *** such rare tumors is crucial due to the potential applicability of tropomyosin receptor kinase inhibitor treatment.

关键词： Uterine sarcoma Cervical sarcoma Neurotrophic tyrosine receptor kinase gene fusion Next generation sequencing Case report

Discovering High-Quality Threaded Discussions in Online Forums

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Journal of Computer Science & Technology 2014年第3期29卷 519-531页

作者： Jung-Tae lee Min-Chul Yang Hae-Chang rim Department of Computer and Radio Communications Engineering Korea UniversitySeoul 136-713Korea

Archives of threaded discussions generated by users in online forums and discussion boards contain valuable knowledge on various topics. However, not all threads are useful because of deliberate abuses, such as trolling and flaming, that are commonly observed in online conversations. The existence of various users with different levels of expertise also makes it difficult to assume that every discussion thread stored online contains high-quality contents. Although finding high-quality threads automatically can help both users and search engines sift through a huge amount of thread archives and make use of these potentially useful resources effectively, no previous work to our knowledge has performed a study on such task. In this paper, we propose an automatic method for distinguishing high-quality threads from low-quality ones in online discussion sites. We first suggest four different artificial measures for inducing overall quality of a thread based on ratings of its posts. We then propose two tasks involving prediction of thread quality without using post rating information. We adopt a popular machine learning framework to solve the two prediction tasks. Experimental results on a real world forum archive demonstrate that our method can significantly improve the prediction performance across all four measures of thread quality on both tasks. We also compare how different types of features derived from various aspects of threads contribute to the overall performance and investigate key features that play a crucial role in discovering high-quality threads in online discussion sites.

关键词： online forum discussion board thread quality

PGLYRP1 protein as a novel mediator of cellular dialogue in neuroinflammation

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Neural Regeneration Research 2025年第7期20卷 1993-1994页

作者： Anup Bhusal Won-Ha lee Kyoungho Suk Department of Pharmacology School of MedicineKyungpook National UniversityDaeguRepublic of Korea BK21 Plus KNU Biomedical Convergence Program Department of Biomedical ScienceSchool of MedicineKyungpook National UniversityDaeguRepublic of Korea School of Life Sciences BK21 plus KNU Creative BioResearch GroupKyungpook National UniversityDaeguRepublic of Korea Brain Science and Engineering Institute Kyungpook National UniversityDaeguRepublic of Korea

Neuroinflammation has been identified as a crucial element in several neurological disorders. Glial cells play a critical role in directing neuroinflammation, both in deleterious and beneficial ways.

关键词： inflammation considerably

Single-cell and spatial omics:exploring hypothalamic heterogeneity

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Neural Regeneration Research 2025年第6期20卷 1525-1540页

作者： Muhammad Junaid Eun Jeong lee Su Bin Lim Department of Biochemistry&Molecular Biology Ajou University School of MedicineSuwonSouth Korea Department of Biomedical Sciences Graduate School of Ajou UniversitySuwonSouth Korea Department of Brain Science Ajou University School of MedicineSuwonSouth Korea

Elucidating the complex dynamic cellular organization in the hypothalamus is critical for understanding its role in coordinating fundamental body functions. Over the past decade, single-cell and spatial omics technologies have significantly evolved, overcoming initial technical challenges in capturing and analyzing individual cells. These high-throughput omics technologies now offer a remarkable opportunity to comprehend the complex spatiotemporal patterns of transcriptional diversity and cell-type characteristics across the entire hypothalamus. Current single-cell and single-nucleus RNA sequencing methods comprehensively quantify gene expression by exploring distinct phenotypes across various subregions of the hypothalamus. However, single-cell/single-nucleus RNA sequencing requires isolating the cell/nuclei from the tissue, potentially resulting in the loss of spatial information concerning neuronal networks. Spatial transcriptomics methods, by bypassing the cell dissociation, can elucidate the intricate spatial organization of neural networks through their imaging and sequencing technologies. In this review, we highlight the applicative value of single-cell and spatial transcriptomics in exploring the complex molecular-genetic diversity of hypothalamic cell types, driven by recent high-throughput achievements.

关键词： cellular diversity hypothalamus multi-omics single-cell transcriptomics spatial transcriptomics

Generation of brain vascular heterogeneity:recent advances from the perspective of angiogenesis

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Neural Regeneration Research 2025年第7期20卷 2013-2014页

作者： Nathanael J.lee Ryota L.Matsuoka Department of Neurosciences Lerner Research InstituteCleveland ClinicClevelandOHUSA Department of Molecular Medicine Cleveland Clinic Lerner College of MedicineCase Western Reserve UniversityClevelandOHUSA

Heterogeneous proper t i es of vascular endothelial cells in the brain:The brain displays large energy dynamics and consumption,and this high level of metabolic demands is fulfilled by a continuous supply of glucose and oxygen through its vascular *** vasculature consists of highly divergent blood vessel branches,giving rise to a dense network of capillaries that supply blood to all cells across the *** elaborated vascular network is thought to develop via angiogenesis,a process in which new blood vessels grow from pre-existing *** capillaries exhibit organotypic features distinct from other tissues and are formed primarily by two major endothelial cell(EC)types:those that form the semi-permeable blood-brain barrier(BBB)and those that develop highly permeable pores known as fenestrae(Matsuoka et al.,2022).The structural and functional differences between BBB and fenestrated vascular ECs represent a fundamental feature of brain vasculature and form the foundation for both brain function and homeostasis.

关键词： angiogenesis homeostasis network

Mechanisms linking gut microbial metabolites to insulin resistance

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World Journal of Diabetes 2021年第6期12卷 730-744页

作者： Hye rim Jang Hui-Young lee Laboratory of Mitochondrial and Metabolic Diseases Department of Health Sciences and TechnologyGAIHSTGachon UniversityIncheon 21999South Korea Korea Mouse Metabolic Phenotyping Center Lee Gil Ya Cancer and Diabetes InstituteGachon UniversityIncheon 21999South Korea Division of Molecular Medicine Department of MedicineGachon University College of MedicineIncheon 21936South Korea

Insulin resistance is the rate-limiting step in the development of metabolic diseases,including type 2 *** gut microbiota has been implicated in host energy metabolism and metabolic diseases and is recognized as a quantitatively important organelle in host metabolism,as the human gut harbors 10 trillion bacterial *** microbiota break down various nutrients and produce metabolites that play fundamental roles in host metabolism and aid in the identification of possible therapeutic targets for metabolic ***,understanding the various effects of bacterial metabolites in the development of insulin resistance is ***,we review the mechanisms linking gut microbial metabolites to insulin resistance in various insulin-responsive tissues.

关键词： Insulin resistance Skeletal muscle Liver Adipose tissue Intestine Gut bacterial metabolites