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Detection of type Ⅳ collagenase activity in malignant ascites

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World Journal of Gastroenterology 2003年第11期9卷 2592-2595页

作者： Xiao-Min Sun Wei-Guo Dong Bao-ping yu He-sheng Luo Jie-ping yu, Department of Gastroenterology, Renmin Hospital, Wuhan University, Wuhan 430060, Hubei Province, China Department of Gastroenterology Renmin Hospital Wuhan University Wuhan 430060 Hubei Province China.

AIM: Type IV collagenase participates in invasion and metastasis of cancer cells. Malignant asdtes is a manifestation of advanced malignant disease that is associated with invasion and metastasis of the peritoneal cavity. Thus, it is reasonable to hypothesize that type IV collagenase is linked to malignant ascites. The purpose of our study was to detect type IV collagenase activity in malignant ascites so as to provide the scientific basis for clinic diagnosis and treatment of malignant ascites. METHODS: Cirrhotic ascites (n=36), tuberculous ascites (n=8) and malignant ascites (n=-23) from patients with gastric cancer (n=6), colon cancer (n=5), ovarian cancer (n=8) and other cancers (n=4), including 2 hepatocellular cancers, 1 pancreatic cancer, 1 primary peritoneal carcinoma were collected by paracentesis. The ascites were made cellfree by centrifugation and stored frozen at -70℃ before determination. Type IV collagenase activity was determined by gelatin zymography. RESULTS: The activity of matrix metalloproteinases-2 and -9 could not be detected in ascites of hepatic cirrhosis and tuberculous peritonitis but could be detected in 20 and 18 out of 23 malignant ascites respectively. The positive rate of type IV collagenase (MMP-2, 87.0% and MMP-9, 78.3%) was higher than that by routine ascites tests (P<0.01) in malignant ascites. Furthermore, the activity of MMP-2 was higher than that of MMP-9 (P=0.022<0.05). CONCLUSION: Type IV collagenase is positive in malignant ascites. Detection of type IV collagenase activity is useful in qualitative diagnosis of ascites. Type IV collagenase may play an important role in malignant ascites formation.

关键词：恶性腹水 IV型胶原酶肿瘤转移恶性肿瘤发病机制

Synthesis and antitumor activity of a group ofdiosgenyl glycosides

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Chinese Chemical Letters 2007年第7期18卷 769-772页

作者： Shu Jie Hou Chuan Chun Zou Liang Zhou Peng Xu De Quan yu ping sheng Lei Key Laboratory of Bioactivity Substance and Resources Utilization of Chinese Herbal Medicine Ministry of EducationInstitute of Materia Medica Peking Union Medical College ＆ Chinese Academy of Medical ScienceBeijing 100050 China

Using orthogonal protection-deprotection glycosylation strategy, a series of diosgenyl glycosides with same of monosaccharides component but different in sequence were synthesized. The antitumor activity in vitro of synthesized diosgenyl glycosides was evaluated by standard MTT assay. It was observed that diosgenyl glycosides with a rhamnopyranosyl at C2′ of glucopyranosyl residue show potent antitumor activity in vitro.

关键词： Diosgenyl glycosides Glycosylation Cytotoxicity

Synthesis and Antitumor Activities of 26-O-tert-Butyldimethylsiliyl Protodisogenyl β-D-Glucopyranoside

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Chinese Chemical Letters 2006年第5期17卷 571-574页

作者： Liang ZHOU Shu Jie HOU Peng XU De Quan yu ping sheng LEI Chuan Chun ZOU Key Laboratory of Bioactive Substance and Resources Utilization of Chinese Herbal Medicine（Peking Union Medical College） Ministry of Education Institute of Materia Medica Peking Union Medical College ＆ Chinese Academy of Medical Sciences Beijing 100050

New saponin 26-O-tert-butyldimethylsiliyl protodiosgenyl β-D-glucopyranoside 1 was synthesized and its cytotoxicity activity in vitro was evaluated by MTI＇. It showed potent antitumor activity to human cancer cells. E ring and C22-OH play important roles in the antitumor activity of 1. A method to selective removal of acetyl group in the presence of benzoyl group was reported.

关键词： Saponin glycosylation cytotoxicity activity structure-activity relationship.

Independent risk factors for depression in older adult patients receiving peritoneal dialysis for chronic kidney disease

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World Journal of Psychiatry 2023年第11期13卷 884-892页

作者： yu-ping sheng Xiao-Ying Ma Ye Liu Xing-Meng Yang Fu-yun Sun Department of Nephrology Cangzhou Central Hospital Cangzhou 061000Hebei ProvinceChina

BACKGROUND According to the trend of global population aging,the proportion of elderly patients with chronic kidney disease(CKD)is expected to ***,there are more than 20 million people in China with decompensated kidney function,of which 19.25%are elderly ***,special attention should be paid to the education years,sleep quality,anxiety status,comorbidities with diabetes,cardiovascular disease(CVD),and anemia as independent risk factors for depression in elderly CKD *** study explores the clinical management of elderly CKD patients that should address these risk factors to prevent depression and improve their *** To investigate depression risk factors in older patients receiving peritoneal dialysis,aiding future prevention of depression in these *** This retrospective study included a primary study population of 170 patients with CKD who received peritoneal dialysis from January 2020 to December *** assessed the patients’mental status using the Beck Depression Inventory Score-II(BDI-II),Self-Rating Anxiety Scale(SAS),Anxiety Inventory Score,and the Pittsburgh Sleep Quality Index(PSQI).Logistic regression was employed to identify depression independent risk factors among these *** The non-depressed group had a significantly longer education period than the depressed group(Pping *** with depression had significantly lower hemoglobin and albumin levels than patients without depression(P<0.05).Spearman correlation analysis of BDI-II scale scores,measuring depression,indicated positive correlations with BDI-II and SAS scores as risk factors for depression in patients with *** contrast,years of education,hemoglobin levels,and peritoneal Kt/V were negatively correlated,serving as protective

关键词： Depression Chronic kidney disease Peritoneal dialysis Older adults Risk factors for depression Beck Depression Inventory Score-II

Effects of paricalcitol combined with hemodiafiltration on bonemetabolism-related indexes in patients with diabetic nephropathy and chronic renal failure

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世界糖尿病杂志:英文版(电子版) 2023年第9期14卷 1385-1392页

作者： Xiao-Ying Ma yu-ping sheng Xing-Meng Yang Hao-Ran Zhang Fu-yun Sun Department of Nephrology Cangzhou Central HospitalCangzhou 061000Hebei ProvinceChina

BACKGROUND Diabetic nephropathy(DN)is frequently seen in the development of diabetes mellitus,and its pathogenic factors are *** current treatment is controversial,and there is a lack of a relevant efficacy prediction *** To determine the effects of paricalcitol combined with hemodiafiltration on bonemetabolism-related indexes in patients with DN and chronic renal failure(CRF),and to construct an efficacy prediction *** We retrospectively analyzed 422 patients with DN and CRF treated in Cangzhou Central Hospital between May 2020 and May *** selected 94 patients who met the inclusion and exclusion *** were assigned to a dialysis group(n=45)and a joint group(n=49)in relation to therapeutic *** clinical efficacy of the two groups was compared after *** changes in laboratory indexes after treatment were evaluated,and the two groups were compared for the incidence of adverse *** predictive value of laboratory indexes on the clinical efficacy on patients was *** The dialysis group showed a notably worse improvement in clinical efficacy than the joint group(P=0.017).After treatment,the joint group showed notably lower serum levels of serum creatinine,uric acid(UA)and blood urea nitrogen(BUN)than the dialysis group(P0.05).According to least absolute shrinkage and selection operator regression analysis,UA,BUN,phosphorus and PINP were related to treatment *** to further comparison,the non-improvement group had higher risk scores than the improvement group(P<0.0001),and the area under the curve of the risk score in efficacy prediction was *** For treatment of CRF and DN,combined paricalcitol a

关键词： Paricalcitol Hemodiafiltration Diabetic nephropathy Chronic renal failure Serum calcium Serum phosphorus Intact Paricalcitol hormone

Volume of fluid simulation of single argon bubble dynamics in liquid steel under RH vacuum conditions

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Journal of Iron and Steel Research International 2024年第4期31卷 828-837页

作者： Gu-jun Chen sheng-ping He College of Materials Science and Engineering Yangtze Normal UniversityChongqing 400044China College of Materials Science and Engineering Chongqing UniversityChongqing 400044China

Single argon bubble dynamics in liquid steel under Ruhrstahl-Heraeus(RH)vacuum conditions were simulated using the volume of fluid method,and the ideal gas law was used to consider bubble growth due to heat transfer and pressure *** simulation with a constant bubble density was also performed to validate the numerical method,and the predicted terminal bubble shape and velocity were found to agree with those presented in the Grace diagram and calculated by drag correlation,*** simulation results under RH conditions indicate that the terminal bubble shape and velocity cannot be *** primary bubble growth occurs within a rising distance of 0.3 m owing to heating by the high-temperature liquid steel;subsequently,the bubble continues to grow under equilibrium with the hydrostatic *** the initial diameter is 8-32 mm,the bubble diameter and rising velocity near the liquid surface are 80-200 mm and 0.5-0.8 m/s,*** bubble rises rectilinearly with an axisymmetric shape,and the shape evolution history includes an initial sphere,(dimpled)ellipsoid,and spherical cap with satellite bubbles.

关键词： Bubble dynamics Liquid steel Volume of fluid Bubble growth Bubble shape Rising velocity

Prediction of liquid circulation flow rate in RH degasser:improvement of decarburization at low atmospheric pressure

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Journal of Iron and Steel Research International 2024年第7期31卷 1661-1667页

The two-fluid model coupled with population balance model was used for simulating the gas-liquid flow in the Ruhrstahl-Heraeus(RH)*** predicted circulation flow rate was compared with that measured from a water model experiment to validate the mathematical ***,influence of snorkel immersion depth on liquid circulation flow rate was numerically investigated under an atmospheric pressure of 101 and 84 kPa,*** result indicates that the circulation flow rate of the RH degasser in the high-altitude area was severely reduced because of the decrease in atmospheric ***,increasing the snorkel immersion depth from 0.5 to 0.7 m can compensate for the decrease in atmospheric *** test result indicates that decarburization rate is significantly enhanced by increasing the snorkel immersion *** optimization,the percentage of heats with a final carbon content less than 0.002 wt.%is significantly increased from 22.0%to 96.4%.

关键词： RH degasser Two-fluid model Population balance model-Circulation flow rate Decarburization Snorkel immersion depth

Long Non-coding RNA PCED1B Antisense RNA 1 Promotes Cell Proliferation and Invasion in Hepatocellular Carcinoma by Regulating the MicroRNA-34a/CD44 Axis

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Current Medical Science 2024年第3期44卷 503-511页

作者： Jian-gang BI Qi LI yu-sheng GUO Li-ping LIU Shi-yun BAO ping XU Department of Hepatobiliary and Pancreatic Surgery Shenzhen People's HospitalThe Second Clinical Medical CollegeJinan UniversityThe First Affiliated HospitalSouthern University of Science and TechnologyShenzhen 518020China Department of Endocrinology Shenzhen People's HospitalThe Second Clinical Medical CollegeJinan UniversityThe First Affiliated HospitalSouthern University of Science and TechnologyShenzhen 518020China

Objective This study aimed to examine the role of long non-coding RNA PCED1B antisense RNA 1(PCED1B-AS1)in the development of hepatocellular carcinoma(HCC).Methods A total of 62 pairs of HCC tissues and adjacent non-tumor tissues were obtained from 62 HCC *** interactions of PCED1B-AS1 and microRNA-34a(miR-34a)were detected by dual luciferase activity assay and RNA pull-down *** RNA expression levels of PCED1B-AS1,miR-34a and CD44 were detected by RT-qPCR,and the protein expression level of CD44 was determined by Western *** cell proliferation was detected by cell proliferation assay,and the cell invasion and migration by transwell invasion *** HCC tumor growth after PCED1B-AS1 was downregulated was determined by in vivo animal *** PCED1B-AS1 was highly expressed in HCC tissues,which was associated with poor survival of HCC ***,PCED1B-AS1 interacted with miR-34a in HCC cells,but they did not regulate the expression of each ***,PCED1B-AS1 increased the expression level of CD44,which was targeted by *** cell proliferation and invasion assay revealed that miR-34a inhibited the proliferation and invasion of HCC in vitro,while CD44 exhibited the opposite ***,PCED1B-AS1 suppressed the role of ***,the knockdown of PCED1B-AS1 repressed the HCC tumor growth in nude mice in *** PCED1B-AS1 may play an oncogenic role by regulating the miR-34a/CD44 axis in HCC.

关键词： long non-coding RNA PCED1B antisense RNA 1(PCED1B-AS1) hepatocellular carcinoma microRNA-34a(miR-34a) CD44 proliferation invasion

Control of gallbladder contractions by cholecystokinin through cholecystokinin-A receptors on gallbladder interstitial cells of cajal

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World Journal of Gastroenterology 2008年第18期14卷 2882-2887页

作者： Dan Xu Bao-ping yu He-sheng Luo Ling-Dan Chen Department of Gastroenterology Renmin Hospital of Wuhan University Wuhan 430060 Hubei Province China

AIM: To identify the cholecystokinin (CCK)-A receptors (CCK-AR) on the guniea pig gallbladder interstitial cells of cajal (ICC) and to study CCK-8 induced gallbladder muscle strip contractions through the CCK-AR. METHODS: The existence of CCK-AR was examined by immunohistofluorescence on sectioned tissue and cultured cells. In vitro contractile response of guinea pig gallbladder muscle strips and the strips with ICC removed were also studied with CCK-8 receptors added. RESULTS: In tissue sections, intensely CCKAR- immunoreactive interstitial cells were found mainly in the muscular layers. In cultured cell sections, distinctive double staining of C-kit and CCK-AR ICCs were found. When we removed the ICC of the gallbladder, CCK-8 induced muscle strip contraction dose response curve significantly shifted to the right. CONCLUSION: We proved that both the existence of CCK-AR on the guinea pig gallbladder ICC and CCK evoked contraction are mediated through direct action on CCK-AR on the gallbladder ICC.

关键词： Gallbladder Interstitial cells of cajal Cholecystokinin-A receptor C-kit Immunohistofluorescence