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[Construction of a 17 β-estradiol sensor based on a magnetic graphene oxide/aptamer separating material]

Se pu = Chinese journal of chromatography 2025年第1期43卷 87-95页

作者： Xin-yu Jin le-yuan Chen ya-Nna Liu Wen-Jing Xie Han-Yong Peng State Key Laboratory of Environmental Chemistry and Ecotoxicology Research Center for Eco-Environmental Sciences Chinese Academy of Sciences Beijing 100085 China. University of Chinese Academy of Sciences Beijing 100049 China. School of Environment Hangzhou Institute for Advanced Study University of Chinese Academy of Sciences Hangzhou 310024 China.

17-Estradiol (E2) is a natural steroidal estrogen essential for a variety of physiological functions in organisms. However, external E2, which is renowned for its potent biological effects, is also considered to be an endocrine-disrupting compound (EDC) capable of disturbing the normal operation of the endocrine system, even at nanogram-per-liter (ng/L) concentrations. Studies have revealed that medical and livestock wastewater can be contaminated with E2, which poses potential risks to human health. Currently, the primary method for detecting E2 relies on liquid chromatography-mass spectrometry, which is limited with regard to on-site or large-scale sample testing due to instrumental constraints. Herein, we developed a magnetic graphene oxide (MgO)/aptamer separating material. The MgO was synthesized by creating a water-in-oil microemulsion at 90 ℃, an agarose hydrogel to load the FeO nanoparticles, and layered graphene oxide (gO). In contrast to conventional methods, such as chemical co-precipitation and solvothermal approaches, this method is more time-efficient and does not require high temperature or pressure. Moreover, the use of a physical encapsulation technique for enwrapping the FeO nanoparticles and layered gO eliminates the need for chemical modification. This approach reduces the use of harmful chemicals, ensures complete loading, and results in highly efficient encapsulation. The MgO was characterized using Fourier-transform infrared (FT-IR) spectroscopy and transmission electron microscopy (TEM), as well as dynamic light scattering (DLS) and Zeta potential analyses, which revealed that the FeO nanoparticles had been successfully loaded onto the gO to produce MgO particles mainly around 5 μm in size. Additionally, this study demonstrated that the aqueous MgO dispersion is highly stable. This substance was used to develop a fluorescent biosensor that uses a "turn-on" mechanism to rapidly and highly sensitively detect E2. MgO is capable of adsorbing a fl

关键词： 17-estradiol (E2) fluorescence biosensor magnetic graphene oxide (MgO) magnetic solid phase separation

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Influence of silicon on the microstructures, mechanical properties and stretch-flangeability of dual phase steels

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International Journal of Minerals,Metallurgy and Materials 2014年第8期21卷 755-765页

作者： le-yu Zhou Dan Zhang ya-zheng Liu School of Materials Science and Engineering University of Science and Technology Beijing

Uniaxial tension tests and hole-expansion tests were carried out to determine the influence of silicon on the microstructures, mechanical properties, and stretch-flangeability of conventional dual-phase steels. Compared to 0.03wt% silicon, the addition of 1.08wt% silicon induced the formation of finer ferrite grains （6.8μm ） and a higher carbon content of martensite （Cm≈ 0.32wt%）. AS the silicon level increased, the initial strain-hardening rate （n value） and the uniform elongation increased, whereas the yield strength, yield ratio, and stretch-flangeability decreased. The microstructures were observed after hole-expansion tests. The results showed that low carbon content martensite （Cm ≈ 0.19wt%） can easily deform in coordination with ferrite. The relationship between the mechanical properties and stretch-flangeability indicated that the steel with large post-uniform elongation has good stretch-flangeability due to a closer plastic incom- patibility of the ferrite and martensite phases, which can effectively delay the production and decohesion of microvoids.

关键词： dual-phase steel silicon content mechanical properties microstructure strain hardening

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Comparative proteomic analysis of plasma exosomes reveals the functional contribution of N-acetyl-alpha-glucosaminidase to Parkinson’s disease

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Neural Regeneration Research 2025年第10期20卷 2998-3012页

作者： yuan Zhao Yidan Zhang Xin Liu Jian Zhang ya.gao Shuyue Li Cui Chang Xiang Liu guofeng ya.g Department of geriatrics Second Hospital of Hebei Medical UniversityShijiazhuangHebei ProvinceChina Department of Neurosurgery The Second Hospital of Hebei Medical UniversityShijiazhuangHebei ProvinceChina

Parkinson’s disease is the second most common progressive neurodegenerative disorder,and few reliable biomarkers are available to track disease *** proteins,DNA,mRNA,and lipids carried by exosomes reflect intracellular changes,and thus can serve as biomarkers for a variety of *** this study,we investigated alterations in the protein content of plasma exosomes derived from patients with Parkinson’s disease and the potential therapeutic roles of these proteins in Parkinson’s *** a tandem mass tag-based quantitative proteomics approach,we characterized the proteomes of plasma exosomes derived from individual patients,identified exosomal protein signatures specific to patients with Parkinson’s disease,and identified N-acetyl-alpha-glucosaminidase as a differentially expressed protein.N-acetyl-alpha-glucosaminidase expression levels in exosomes from the plasma of patients and healthy controls were validated by enzyme-linked immunosorbent assay and western *** results demonstrated that the exosomal N-acetyl-alpha-glucosaminidase concentration was not only lower in Parkinson’s disease,but also decreased with increasing Hoehn-yahr stage,suggesting that N-acetyl-alpha-glucosaminidase could be used to rapidly evaluate Parkinson’s disease ***,western blot and immunohistochemistry analysis showed that N-acetyl-alpha-glucosaminidase levels were markedly reduced both in cells treated with 1-methyl-4-phenylpyridinium and cells overexpressingα-synuclein compared with control ***,N-acetyl-alpha-glucosaminidase overexpression significantly increased cell viability and inhibitedα-synuclein expression in 1-methyl-4-phenylpyridinium-treated *** together,our findings demonstrate for the first time that exosomal N-acetyl-alpha-glucosaminidase may serve as a biomarker for Parkinson’s disease diagnosis,and that N-acetyl-alpha-glucosaminidase may reduceα-synuclein expression and 1-methyl-4-phenylpyridinium-induced n

关键词： biomarker diagnosis exosomes N-acetyl-alpha-glucosaminidase Parkinson’s disease proteomic α-synuclein

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Double [5]carbohelicene: facile synthesis, chiroptical properties,isomerization study, and lasing application

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Science China Chemistry 2025年第1期 233-240页

作者： Peipei Liu yantong Li Meng-Xiang Wu Hao Kang Xiao-Li Zhao Lin Xu Linlin Liu Xiaodong Li Junfeng Fang Zhiwei Fang ya Cheng Hai-Bo ya.g Huakang yu Xueliang Shi State Key Laboratory of Petroleum Molecular & Process Engineering Shanghai Key Laboratory of Green Chemistry and Chemical ProcessesSchool of Chemistry and Molecular Engineering East China Normal University School of Physics and Optoelectronics South China University of Technology Institute of Polymer Optoelectronic Materials & Devices State Key Laboratory of Luminescent Materials & Devices South China University of Technology School of Physics and Electronic Science Engineering Research Center of Nanophotonics & Advanced Instrument Ministry of EducationEast China Normal University State Key Laboratory of Precision Spectroscopy School of Physics and Electronic Science East China Normal University

Double carbohelicenes have attracted considerable attention due to their aesthetic structures, distinct π-conjugation extension, inherent chirality, and intriguing optical and electronic properties. Herein, the concise de novo synthesis of a new double[5]carbohelicene 1 together with its chiroptical properties, isomerization process and lasing application is presented. 1 was synthesized by a simple protocol involving the formation of a 6,6′-bipentacene as the key intermediate. It is worth noting that1 existed as two diastereomers 1-PP/MM and 1-PM, which were successfully isolated and unambiguously confirmed by Xray crystallography. The optical resolution of the racemic 1-PP/MM was successfully achieved by chiral-phase highperformance liquid chromatography（HPLC）, and 1-PP and 1-MM were characterized by circular dichroism. Interestingly, it was found that 1-PP/MM could be completely isomerized to 1-PM upon heating, and the detailed thermodynamics and kinetics of the isomerization process were systematically investigated. 1-PM exhibited deep-red luminescence with emission maximum at 723 nm and fluorescence quantum yield as high as 32.95%. Consequently, we can showcase the lasing application of 1-PM by doping with epoxy resin to assemble a bottle microlaser spontaneously, which the lasing wavelength is 796.6 nm at a threshold of 0.8 m J cm-2. We believe that our studies, including the facile synthesis methodology, detailed isomerization studies, and lasing application, will shed some light on the design and synthesis of novel carbohelical systems with more functions.

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Surface decarburization characteristics and relation between decarburized types and heating temperature of spring steel 60Si2MnA

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International Journal of Minerals,Metallurgy and Materials 2013年第8期20卷 720-724页

作者： Chao-lei Zhang le-yu Zhou ya-zheng Liu School of Materials Science and Engineering University of Science and Technology Beijing

The characteristics of complete and partial decarburizations in spring steel 60Si2MnA were systematically investigated, including the microstructure, the hardness gradient, and the formation mechanism. The relation between decarburized types and heating temperature of the steel was comprehensively discussed. It is found that heating temper- ature has an important influence on the decarburized types. With the rise of heating temperature, the decarburized types change from no decarburization to complete decarburization, complete and partial decarburizations, partial decarburiza- tion, and no decarburization.

关键词： spring steel decarburization temperature microstructure hardness

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Physical Layer Security Scheme With AoI-Awareness for Industrial IoT Based on Covert Communications

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IEEE/CAA Journal of Automatica Sinica 2025年第1期 276-278页

作者： yaping Li Zhi-Xin Liu Jia-Wei Su ya-Zhou yuan the Key Laboratory of Industrial Computer Control Engineering of Hebei Province Yanshan University

Dear Editor,Industrial Internet of things(IIoT) is a typical application of cyberphysical system(CPS). In the IIoT, wireless communication is an inevitable trend to replace the deployment-limited wired transmission for cases with large-scale and mobile devices. However, wireless communication gives rise to critical issues related to physical security, such as malicious detections and attacks [1].

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BIOLOgICAL INVASION PROBleM WITH FREE BOUNDARY NONLOCAL DIFFUSION EQUATION

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数学杂志 2025年第1期45卷 48-56页

作者： HE yu-rong ZHANg ya-rong School of Science Xi'an University of Architecture and TechnologyXi'an 710055China Xi'an Special Equipment Inspection Institute Xi'an 710065China School of Power and Energy Northwestern Polytechnical UniversityXi'an 710072China

In order to better describe the phenomenon of biological invasion,this paper introduces a free boundary model of biological ***,the right free boundary is added to the equation with logistic ***,the existence and uniqueness of local solutions are proved by the Sobolev embedding theorem and the comparison ***,according to the relevant research data and contents of red fire ants,the diffusion area and nest number of red fire ants were simulated without external *** paper mainly simulates the early diffusion process of red fire *** the early diffusion stage,red fire ants grow slowly and then spread over a large area after reaching a certain number.

关键词： Free boundary biological invasion differential equation

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Assessment of the cropland classifications in four global land cover datasets： A case study of Shaanxi Province, China

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Journal of Integrative Agriculture 2017年第2期16卷 298-311页

作者： CHEN Xiao-yu LIN ya ZHANg Min yu le LI Hao-chuan BAI yu-qi Key Laboratory for Earth System Modelling Ministry of Education/Department of Earth System Science (DESS) Tsinghua University Beijing 100084 P.R. China National Information Center Beijing 100045 P.R. China

Accurate and reliable cropland surface information is of vital importance for agricultural planning and food security monitoring. As several global land cover datasets have been independently released, an inter-comparison of these data products on the classification of cropland is highly needed. This paper presents an assessment of cropland classifications in four global land cover datasets, i.e., moderate resolution imaging spectrometer （MODIS） land cover product, global land cover map of 2009 （globCover2009）, finer resolution observation and monitoring of global cropland （FROM-gC） and 30-m global land cover dataset （globeLand30）. The temporal coverage of these four datasets are circa 2010. One of the typical agricultur- al regions of China, Shaanxi Province, was selected as the study area. The assessment proceeded from three aspects： accuracy, spatial agreement and absolute area. In accuracy assessment, 506 validation samples, which consist of 168 cropland samples and 338 non-cropland ones, were automatically and systematically selected, and manually interpreted by referencing high-resolution images dated from 2009 to 2011 on google Earth. The results show that the overall accuracy （OA） of four datasets ranges from 61.26 to 80.63%. globeLand30 dataset, with the highest accuracy, is the most accurate dataset for cropland classification. The cropland spatial agreement （mainly located in the plain ecotope of Shaanxi） and the non-cropland spatial agreement （sparsely distributed in the south and middle of Shaanxi） of the four datasets only makes up 33.96% of the whole province. FIROM-gC and globeLand30, obtaining the highest spatial agreement index of 62.40%, have the highest degree of spatial consistency. In terms of the absolute area, MODIS underestimates the cropland area, while globCover2009 significantly overestimates it. These findings are of value in revealing to which extent and on which aspect that these global land cover datasets may agree with each other at

关键词： land cover cropland classification assessment MODIS globCover2009 FROM-gC globeLand30

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Failure analysis of 18CrNi3MoA drilling tails

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International Journal of Minerals,Metallurgy and Materials 2012年第5期19卷 421-427页

作者： Hong-wu Zhu ya-zheng Liu le-yu lhobl lei-ying Wang Bin Huang School of Materials Science and Engineering University of Science and Technology Beijing Beijing 100083 China Xining Special Steel Co. Ltd. Xining 810005 China

The fracture of a drilling tail made of 18CrNi3MoA steel in the exchanging water hole was analyzed in terms of inclusions, prior austenite grain size, carburized layers, and fatigue fracture morphology by means of optical microscopy, quantitative metallography, and scanning electron microscopy （SEM）. Fatigue crack initiation and propagation on the drilling tail were also studied. The results showed that the fracture on the drilling tail was not induced by inclusions and the distribution inhomogeneity of prior austenite grain size. Instead, be- cause the outside surface of the exchanging water hole was not continuously geometric, there was a great deal of stress concentration in those areas under continuous twisting, axial impact, and corrosion of mineral water. Thus three crack sources emerged in these areas. Initial cracks centered on these sources spread from the outside surface to the inside surface, and eventually the drilling tail ruptured. Furthermore, the fractograph of the region around the crack sources exhibited a typical ductile dimple fracture pattern, and cracks initiated on the outside sur- face of the carburized layers of the exchanging water hole. Three crack sources could be the sources of fatigue crack propagation. Based on the analysis of fatigue crack propagation, it was further demonstrated that fatigue damage originated from the outside surface of the ex- changing water hole.

关键词： drilling tools failure analysis crack initiation crack propagation

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Forming condition and control strategy of ferrite decarburization in 60Si2MnA spring steel wires for automotive suspensions

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International Journal of Minerals,Metallurgy and Materials 2012年第2期19卷 116-121页

作者： Chao-lei Zhang ya-zheng Liu le-yu Zhou Chao Jiang Jin-fu Xiao School of Materials Science and Engineering University of Science and Technology BeijingBeijing 10083China

The ferrite decarburization behavior of 60Si2MnA spring steel wires for automotive suspensions, including the forming condition and the influence of heating time and cooling rate after hot rolling, was investigated comprehensively. Also, a control strategy during the reheating process and cooling process after rolling was put forward to protect against ferrite decarburization. The results show that ferrite decarburization, which has the strong temperature dependence due to phase transformation, is produced between 675 and 875°C. The maximum depth is found at 750°C. Heating time and cooling rate after rolling have an important influence on decarburization. Reasonable preheating temperature in the billet reheating process and austenitizing temperature in the heat-treatment process are suggested to protect against ferrite decarburization.

关键词： spring steel decarburization phase transformations controlled cooling

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