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Production of chitosan scaffolds by lyophilization or electrospinning: which is better for peripheral nerve regeneration?

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Neural Regeneration Research 2021年第6期16卷 1093-1098页

作者： yu-Xuan Wu Hao Ma Jian-lan Wang Wei qu Department of Hand Microsurgery First Affiliated Hospital of Dalian Medical UniversityDalianLiaoning ProvinceChina Department of Plastic and Reconstructive Surgery Shanghai Ninth People’s HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghaiChina Department of Traditional Chinese Medicine First Affiliated Hospital of Dalian Medical UniversityDalianLiaoning ProvinceChina

Both lyophilization and electrospinning are commonly used to make chitosan scaffolds.However,it remains unknown which method is better for cell growth.In this study,we established the following groups:(1)lyophilization group-chitosan scaffolds were prepared by lyophilization method and seeded with Schwann cells from Sprague-Dawley rats aged 3-5 days;(2)electrospinning group-chitosan scaffolds were prepared by electrospinning method and seeded with Schwann cells;(3)control group-Schwann cells were cultured on culture dishes.The growth of Schwann cells was evaluated by immunofluorescence and scanning electron microscopy.Western blot assay was performed to explore the mechanism of Schwann cell growth.Both materials were non-toxic and suitable for the growth of Schwann cells.The pores produced by electrospinning were much smaller than those produced by lyophilization.The proliferation rate and adhesion rate of Schwann cells in the electrospinning group were higher than those in the lyophilization group.Schwann cells cultured on electrospinning scaffolds formed a Bungner band-like structure,and a much greater amount of brain-derived neurotrophic factor was secreted,which can promote the growth of neurons.Our findings show that the chitosan scaffold prepared by the electrospinning method has a nanofiber structure that provides an extracellular matrix that is more favorable for cell-cell interactions.The electrospinning method is more suitable for nerve regeneration than the lyophilization method.This research was approved by the Medical Ethical Committee of Dalian Medical University(approval No.AEE1-2016-045)on March 3,2016.

关键词： cells factor growth in vitro model peripheral nerve plasticity protein rat regeneration

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Citation Classics in Asthma Research： The 100 Top-Cited Articles During 1960-2014

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Chinese Medical Journal 2018年第9期131卷 1115-1116页

作者： Sha Li Cheng-Jie Zhu Yu-Lan Qu yu-Chao Dong Yan Shang Chong Bai Department of Respiratory and Critical Care Medicine Changhai HospitalSecond Military Medical University Shanghai 200433China Section of Health No. 94804 Unit of People's Liberation Army Shanghai 200434 China Department of Respiratory Medicine Haining People's Hospital of Zhejiang Province Haining Zhejiang 314400 China

To the Editor：Asthma poses a serious health problem globally.People of all ages can be affected by this chronic airway disorder that,when uncontrolled,may place severe limits on daily life and is sometimes fatal.The prevalence of asthma is increasing in most countries throughout the world,especially among children.[1]During the past decades,many scientific advances have been made to improve our understanding of asthma and our ability to manage and control it effectively.These advances are mainly reflected by related scientific articles,particularly by those most influential papers.

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Association between Genetic Variants of Transforming Growth Factor-β1 and Susceptibility of Pneumoconiosis： A Meta-analysis

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Chinese Medical Journal 2017年第3期130卷 357-364页

作者： Chang-Wen Deng Xing-Xing Zhang Jin-Huan Lin Li-Fei Huang Yu-Lan Qu Chong Bai Department of Respiratory and Critical Care Medicine Changhai Hospital The Second Military Medical University Shanghai 200433 China Department of Cell Biology and Stem Cell Research Center School of Basic Medical Sciences Peking University Health Science Center Beijing 100191 China Department of Gastroenterology Changhai Hospital The Second Military Medical University Shanghai 200433 China Department of Respiratory Haining People's Hospital Jiaxing Zhejiang 314400 China

Background： Transforming growth factor-beta 1 （TGF-β 1） and gene variants have been extensively studied in various human diseases. For example, TGF-β1 polymorphisms were associated with fibrosis and pneumoconiosis, but the data remained controversial. The aim of this meta-analysis was to assess the association between TGF-β1-509 C〉T [rs 1800469], ＋869 T〉C [rs 1800470], and ＋915 G〉C [rs 1800471 ] polymorphisms and pneumoconiosis, Methods： A comprehensive literature search was conducted through searching in PubMed, Embase, the Chinese Biomedical Database, and the Wei Pu （Chinese） Database by the end of April 2016. Eleven publications with 21 studies were included in this recta-analysis, covering a total of 4333 patients with pneumoconiosis and 3478 controls. Study quality was assessed, and heterogeneity and publication bias were measured. All statistical analyses were performed using STATA version 12.0 （StataCorp, College Station, TX, USA） software. Results： The data showed significant associations between TGF-β1-509 C〉T polymorphism and the risk ofpneumoconiosis development （T vs. C, odds ratio [OR] = 1.35, 95% confidence interval [CI]： 1.00-1.81, P = 0.046）; between TGF-fll ＋915 G〉C polymorphism and the pneumoconiosis risk （C vs. G, OR = 1.69, 95% CI： 1.19-2.40, P = 0.004; CG vs. GG, OR = 1.79, 95% CI： 1.23-2.60, P = 0.002; CC＋CG vs. GG, OR = 1.80, 95% CI： 1.24-2.61, P = 0.002）. In addition, the subgroup analysis of ethnicity versus pneumoconiosis types indicated a significant association of silicosis among Asian populations but not that of coal workers＇ pneumoconiosis in Caucasian populations. In contrast, no significant association was exhibited between TGF-β1 ＋869 T〉C polymorphism and risk ofpneumoconiosis. Conclusion： The polymorphisms of both TGF-β1 -509 C〉T and ＋915 G〉C are associated with increased risk of pneumoconiosis. Key words： Meta analysis; Pneumoconiosis; Polymorphism; Transforming Growth Factor-betal

关键词： Meta analysis Pneumoconiosis Polymorphism Transforming Growth Factor-betal

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Nanoscale multi-beam lithography of photonic crystals with ultrafast laser

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光:科学与应用(英文版) 2023年第8期12卷 1604-1615页

作者： Jiaqun Li Jianfeng Yan lan Jiang Jiachen yu Heng Guo Liangti qu State Key Laboratory of Tribology in Advanced Equipment Department of Mechanical EngineeringTsinghua UniversityBeijing 100084China School of Mechanical Engineering Beijing Institute of TechnologyBeijing 100081China Department of Chemistry Tsinghua UniversityBeijing 100084China

Photonic crystals are utilized in many noteworthy applications like optical communications,light flow control,and quantum optics.Photonic crystal with nanoscale structure is important for the manipulation of light propagation in visible and near-infrared range.Herein,we propose a novel multi beam lithography method to fabricate photonic crystal with nanoscale structure without cracking.Using multi-beam ultrafast laser processing and etching,parallel channels with subwavelength gap are obtained in yttrium aluminum garnet crystal.Combining optical simulation based on Debye diffraction,we experimentally show the gap width of parallel channels can be controlled at nanoscale by changing phase holograms.With the superimposed phase hologram designing,functional structures of complicated channel arrays distribution can be created in crystal.Optical gratings of different periods are fabricated,which can diffract incident light in particular ways.This approach can efficiently manufacture nanostructures with controllable gap,and offer an alternative to the fabrication of complex photonic crystal for integrated photonics applications.

关键词： lithography beam optics

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Mechanistic investigation of zwitterionic MOF-catalyzed enyne annulation using UNLPF-14-Mn^(Ⅲ) as catalyst

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Chinese Chemical Letters 2022年第9期33卷 4281-4286页

作者： Taotao Liu Ruihong Duan Yanyan Wang Shijun Li Lingbo qu Jinshuai Song Qiang Liu yu lan Green Catalysis Center and College of ChemistryZhengzhou UniversityZhengzhou 450001China Center of Basic Molecular Science(CBMS) Department of ChemistryTsinghua UniversityBeijing 100084China College of Chemistry and Chemical Engineering Henan Institute of Science and TechnologyXinxiang 453000China School of Chemistry and Chemical Engineering and Chongqing Key Laboratory of Theoretical and Computational ChemistryChongqing UniversityChongqing 400030China

Hybrid quantum mechanics/molecular mechanics calculations were performed to elucidate how[Mn^(Ⅲ)porphyrin]^(+X−)-based metal-organic frameworks(MOFs)catalyze the[2+1]cycloisomerization of enynes and why zwitterionic MOFs exhibit strong activity in Lewis acid catalysis.The calculations showed that zwitterionic MOFs have a“pure cationic active center”leading to a concerted nucleophilic attack pathway with lower barriers.In contrast,metals with coordinating anions have reduced electrophilicity,resulting in a stepwise radical-type pathway with much higher barriers.Further calculations showed the nature of catalysis was strongly depended on the charge on the anion ligand.A good linear relationship between the NPA charge and barrier was found,and verified by 73 anions with small derivations,which presents a universal adaptive character for various coordinated anions.

关键词： ONIOM QM/MM Zwitterionic MOF [Mn^(III)porphyrin]^(+X−) Enyne annulation Mechanism Anion effect

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Thermoresponsive block copolymer supported Pt nanocatalysts for base-free aerobic oxidation of 5-hydroxymethyl-2-furfural

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Frontiers of Chemical Science and Engineering 2021年第6期15卷 1514-1523页

作者： Huaxin qu Jie Deng Bei Wang Lezi Ouyang Yong Tang Kai yu lan-lan Lou Shuangxi Liu Institute of New Catalytic Materials Science School of Materials Science and EngineeringNational Institute for Advanced MaterialsNankai UniversityTianjin300350China MOE Key Laboratory of Pollution Processes and Environmental Criteria and Tianjin Key Laboratory of Environmental Technology for Complex Transmedia Pollution College of Environmental Science and EngineeringNankai UniversityTianjin300350China MOE Key Laboratory of Advanced Energy Materials Chemistry Collaborative Innovation Center of Chemical Science and Engineering(Tianjin)Tianjin300072China

A base-free catalytic system for the aerobic oxidation of 5-hydroxymethyl-2-furfural was exploited by using Pt nanoparticles immobilized onto a thermoresponsive poly(acrylamide-co-acrylonitrile)-b-poly(N-vinylimidazole)block copolymer,with an upper critical solution temperature of about 45°C.The Pt nanocatalysts were well-dispersed and highly active for the base-free oxidation of 5-hydroxymethyl-2-furfural by molecular oxygen in water,affording high yields of 2,5-furandicarboxylic acid(up to>99.9%).The imidazole groups in the block copolymer were conducive to the improvement of catalytic performance.Moreover,the catalysts could be easily separated and recovered based on their thermosensitivity by cooling the reaction system below the upper critical solution temperature.Good stability and reusability were observed over these copolymer-immobilized catalysts with no obvious decrease in catalytic activity in the five consecutive cycles.

关键词： aerobic oxidation base-free 5-hydroxymethyl-2-furfural Pt nanoparticle thermoresponsive block copolymer

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Geometry, stability and aromaticity of β-diketiminate-coordinated alkaline-earth compounds

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Chinese Chemical Letters 2019年第12期30卷 2249-2253页

作者： yuanyuan Li Haohua Chen Lingbo qu Ruopeng Bai yu lan Department of Biological and Chemical Engineering Chongqing University of EducationChongqing 400067China Cooperative Innovation Center of Lipid Resources and Children’s Daily Chemicals Chongqing University of EducationChongqing 400067China School of Chemistry and Chemical Engineering Chongqing UniversityChongqing 401331China College of Chemistry and Molecular Engineering Zhengzhou UniversityZhengzhou 450006China

Alkaline-earth(Ae) metals have attracted a wealth of interdependent research from synthetic chemists.In Ae-catalyzed organometallic reactions,β-diketiminate is a typical ligand used to stabilize Ae catalysts by forming six-membered rings comprising Ae metals.Herein,studies focusing on the configuration of β-diketiminate-coordinated Ae compounds observed that the C-C and C-N bonds are homogeneous and unchanged.Furthermore,energetic studies observed that the formation of the Ae-incorporated sixmembered rings results in enhanced stability of>20 kcal/mol.The nucleus-independent chemical shifts,anisotropy of the induced current density,and molecular orbital analyses demonstrated the nonaromaticity of the β-diketiminate-coordinated Ae compounds.The improved stability of these compounds can be explained by the delocalization of the π electrons derived from the β-diketiminate moiety.

关键词： β-Diketiminate-coordinated Alkaline-earth compounds Theoretical study Aromaticity Stability

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Regioselectivity of Pd-catalyzed o-carboranearylation: a theoretical view

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Organic Chemistry Frontiers 2022年第6期9卷 1686-1695页

作者： Jiying Liu Dongmin Fu Zitong Chen Tiantian Li Ling-Bo qu Shi-Jun Li Wenjing Zhang yu lan Green Catalysis Center and College of ChemistryZhengzhou UniversityZhengzhouHenan 450001China School of Chemistry and Chemical Engineering and Chongqing Key Laboratory of Theoretical and Computational ChemistryChongqing UniversityChongqing 400030China

The mechanism and the regioselectivity of Pd-catalyzed B(4)–H arylation and B(4,5)–H diarylation foro-carborane with bidentate directing groups were investigated. The complete catalytic cycle involves Pdmediated concerted metalation–deprotonation, oxidative addition, counterion exchange, and reductiveelimination, where counterion exchange is critical. The reductive elimination is the rate- and regioselectivity-determining step for the whole catalytic cycle. B(3)-Arylation is unfavorable because the stericrepulsion between the substituent group on C(2) and the metal moiety would lead to significant distortionof o-carborane and would result in a higher activation energy for reductive elimination. The distortion ofo-carborane can be clearly observed from the change of its aromaticity in the corresponding transitionstates by the calculation of nucleus-independent chemical shifts. Moreover, counterion exchange withcarboxylate before reductive elimination significantly increases the positive charge of palladium, whichleads to a lower activation free energy for the sequential step.

关键词： elimination catalyzed reductive

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Theoretical insight into phosphoric acid-catalyzed asymmetric conjugate addition of indolizines to a,β-unsaturated ketones

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Chinese Chemical Letters 2018年第8期29卷 1237-1241页

作者： Haohua Chen Lei Zhu Kangbao Zhong Xiaoyu yue Ling-Bo qu Ruopeng Bai yu lan School of Chemistry and Chemical Engineering Chongqing University College of Chemistry and Molecular Engineering Zhengzhou University

Phosphoric acid catalysis is a powerful tool for the construction of new CààC bonds because of its unique LUMO-lowering activity. Theoretical calculations were employed to investigate phosphoric acidcatalyzed asymmetric conjugate addition of indolizines to a,b-unsaturated ketones. The calculation results showed that this transformation proceeds via a reaction pathway involving nucleophilic addition,deprotonation–aromatization, and tautomerization. The computational results showed that deprotonation–aromatization is the rate-determining step and the enantioselectivity-determining step.The S-configured product was preferentially generated via a pathway starting from the s-cis conjugated ketone, whereas the s-trans isomer led to a side product with the R configuration. Non-covalent interaction analysis showed that the enantioselectivity is mainly caused by bond-rotation strain in the transition states of the deprotonation–aromatization step.

关键词： Phosphoric acid catalysis Theoretical calculations Mechanism Enantioselectivity

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间充质干细胞在支气管哮喘治疗中的应用:挑战与策略

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海军军医大学学报 2022年第6期43卷 589-596页

作者：屈玉兰戈霞晖白冲海军军医大学(第二军医大学)第一附属医院呼吸与危重症医学科上海200433 上海中医药大学上海市第七人民医院呼吸内科上海200137

支气管哮喘(简称哮喘)缺乏特别有效的治疗手段。间充质干细胞(MSC)因具有抑制炎症、改善气道重塑的作用及可经呼吸道给药等优势,在哮喘治疗领域被广泛研究。虽然近年来对MSC治疗哮喘的应用性探索不断涌现,但其治疗哮喘的临床试验结果并... 详细信息

支气管哮喘(简称哮喘)缺乏特别有效的治疗手段。间充质干细胞(MSC)因具有抑制炎症、改善气道重塑的作用及可经呼吸道给药等优势,在哮喘治疗领域被广泛研究。虽然近年来对MSC治疗哮喘的应用性探索不断涌现,但其治疗哮喘的临床试验结果并不理想。不同类型的干细胞、不同哮喘患者的病理生理特征的差异给MSC的临床应用带来了严峻的挑战。本文就目前MSC治疗哮喘的作用机制研究及临床应用挑战进行回顾分析,以更好地了解MSC治疗哮喘的潜力及临床应用的可行性。

关键词：哮喘间充质干细胞免疫调节临床应用

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