检索结果-南通市图书馆

Particulate capillary precolumns with double-end polymer monolithic frits for on-line peptide trapping and preconcentration

在线全文

学校读者我要写书评

暂无评论

Chinese Chemical Letters 2015年第9期26卷 1068-1072页

作者： Si-Min Xia Hui-Ming yuan Zheng Liang Li-Hua zhang yu-kui zhang University of Chinese Academy of Science National Chromatographic R&A Center Dalian Institute of Chemical PhysicsThe Chinese Academy of Sciences

In this work,a novel kind of particulate capillary precolumns with double-end polymer monolithic frits has been ***,the polymer monolithic frit at one end was prepared via photo-initiated polymerization of a mixture of lauryl methacrylate and ethyleneglycol dimethacrylate with 1-propanol and 1,4-butanediol as porogens and 2,2-dimethoxy-2-phenylacetophenone as a photo-initiator in UV transparent coating capillary（100 μm i.d.）.Subsequently,C18 particles（5 μm,100 A） were packed into the capillary,and sealed with the polymer monolithic frit at another *** prevent the reaction of monomers and C18 particles,the packed C18 particles were masked during UV *** loading capacity of such a precolumn was determined to be about 9 μg by frontal analysis with a synthetic peptide APGDR1 YVHPF as a model ***,two parallel precolumns were incorporated into a two-dimensional nano-liquid chromatography（2D nano-LC） system with dual capillary trap columns for peptide trapping and *** to 2D nano-LC system with a single trap column,such two dimensional separations could be operated simultaneously to improve the analysis *** these results demonstrated that such capillary precolumns with double frits would be promising for high-throughput proteome analysis.

关键词： Capillary precolumn Double-end frits Peptide trapping 2D nano-LC

Preparation of poly（N-isopropylacrylamide） brush grafted silica particles via surface-initiated atom transfer radical polymerization used for aqueous chromatography

在线全文

学校读者我要写书评

暂无评论

Frontiers of Materials Science 2012年第1期6卷 60-68页

作者： Zong-Jian LIU Yan-Li LIANG Fang-Fang GENG Fang LV Rong-Ji DAI yu-kui zhang yu-Lin DENG School of Life Science Beijing Institute of TechnologyBeijing 100081China Dalian Institute of Chemical Physics Chinese Academy of SciencesDalian 116023China

Thermoresponsive poly（N-isopropylacrylamide）（PNIPAAm） brushes were densely grafted onto silica surface via surface-initiated atom transfer radical polymeriza- tion （SI-ATRP）. The grafting reaction started from the surfaces of 2-bromoisobutyrate- functionalized silica particles in 2-propanol aqueous solution at ambient temperature using CuCIICuCI21N, N,N＇,N＇,N”.pentamethyldiethylenetriamine （PMDETA） as the catalytic system. Based on thermogravimetric analysis （TGA） results, the grafting amount and grafting density of PNIPAM chains on the surface of silica were calculated to be 1.29 mg/ m^2 and 0.0215 chains/nm^2, respectively. The gel permeation chromatography （GPC） result showed the relatively narrow molecular weight distribution （MwlMn= 1.21） of the grafted PNIPAAm. The modified silica particles were applied as high-performance liquid chromatography （HPLC） packing materials to successfully separate three aromatic compounds using water as mobile phase by changing column temperature. Temperature- dependent hydrophilic/hydrophobic property alteration of PNIPAAm brushes grafted on silica particles was determined with chromatographic interaction between stationary phase and analytes. Retention time was prolonged and resolution was improved with increasing temperature. Baseline separation with high resolution at relatively low temperatures was observed, demonstrating dense PNIPAAm brushes were grafted on silica surfaces.

关键词： poly(N-isopropylacrylamide) (PNIPAAm) atom transfer radical polymeriza-tion (ATRP) temperature-responsive chromatography separation

Label-Free Quantitative Proteomics Analysis of the Sorafenib Resistance in HepG2 Cells

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Analysis and Testing 2022年第3期6卷 308-317页

作者： Zi-Xuan Wang Hong-Wei Chu Kai-Guang Yang Bao-Feng Zhao Zhen Liang Li-Hua zhang yu-kui zhang CAS Key Laboratory of Separation Science for Analytical Chemistry National Chromatographic R.&A.CenterDalian Institute of Chemical PhysicsChinese Academy of ScienceDalian 116023China University of Chinese Academy of Sciences Beijing 100039China zhang Dayu School of Chemistry Dalian University of TechnologyDalian 116024China

Drug resistance of sorafenib seriously affects the treatment effect of late-stage hepatocellular carcinoma(HCC)***,the precise mechanism of resistance to sorafenib remains ***,to obtain a deep understand of sorafenib resistance mechanisms and find potential therapeutic targets are very important for improving the clinical prognosis of HCC *** this study,a label-free quantitative proteomics method was performed to investigate the proteins differentially expressed between HepG2 and the sorafenib-acquired resistance HepG2(HepG2-R)*** total,84 differential expressed proteins were identified between the two cell *** analysis results demonstrated the dysregulated metabolic processes have a significant impact on the drug resistance of HepG2-R *** them,the expression of Microsomal glutathione S-transferase 1(MGST1)in two cell lines was further confirmed by western blot ***,colony formation assay and trypan blue dye assay results revealed that MGST1 is closely connected with the sorafenib resistance of HepG2-R cells,and the knockdown of MGST1 increased the sensitivity of sorafenib resistance HepG2-R cells to sorafenib *** conclusion,these results lay a foundation for deciphering the mechanism for HCC sorafenib resistance and present a possibility of MGST1 serving as a therapeutic target for the treatment of sorafenib resistance HCC.

关键词： Drug resistance Hepatocellular carcinoma Quantitative proteomics Sorafenib

Troy: Efficient Service Deployment for Windows Systems

在线全文

学校读者我要写书评

暂无评论

Chinese Journal of Electronics 2024年第1期33卷 313-322页

作者： Deyu zhang yu XIE Mucong XU En CHENG Xiaoyan kui Bangwen HE yunhao LI School of Computer Science and Engineering Central South University

The modern university computer lab and kindergarden through 12th grade classrooms require a centralized solution to efficiently manage a large number of desktops. The existing solutions either bring virtualization overhead in runtime or requires loading a large image over 30 GB leading to an unacceptable network latency. In this work, we propose Troy which takes advantage of the differencing virtual hard disk techniques in Windows *** such, Troy only loads the modifications made on one machine to all other machines. Troy consists of two modules that are responsible to generate an initial image and merge a differencing image with its parent image, respectively. Specifically, we identify the key fields in the virtual hard disk image that links the differencing image and the parent image and find the modified blocks in the differencing images that should be used to replace the blocks in the parent image. We further design a lazy copy solution to reduce the I/O burden in image merging. We have implemented Troy on bare metal machines. The evaluation results show that the performance of Troy is comparable to the native implementation in Windows, without requiring the Windows environment.

关键词： Runtime Image synthesis Merging Loading Metals Hard disks Virtualization

同方期刊数据库

Enantiomeric separations of four basic drugs containing N-alkyl groups by a RP-HPLC system using SBE-β-CD as chiral mobile phase additive

在线全文

学校读者我要写书评

暂无评论

Chinese Chemical Letters 2013年第9期24卷 840-844页

作者： Min Huang Wen-Jing Chen Ying Zhou Ru Feng Jie Fu Jing-Yi Ma Xiang-Shan Tan Chi-yu He Qi-Ming zhang Wen-Yi He yu-Lin Deng yu-kui zhang Xian-Feng zhang Yan Wang State Key Laboratory of Bioactive Substances and Functions of Natural Medicines Institute of Materia Medica Chinese Academy of Medical Sciences & Peking Union Medical College National Institute for the Control of Pharmaceutical and Biological Products School of Life Science Beijing Institute of Technology Department of Neurosurgery First Hospital Jilin University

The chiral separations of four pharmaceutical racemates which contain N-alkyl groups were satisfactorily resolved using SBE-β-CD as a chiral mobile phase additive （CMPA） in a RP-HPLC system （the resolution is 2.701 for ondansetron hydrochloride, 1.996 for sulpiride, 1.293 for clenbuterol hydrochloride and 0.816 for omeprazole）. In addition, the effects of different parameters such as CD type and CD concentration were investigated. The separation mechanism arises through the combination of several potential interactions, including electrostatic interactions as well as hydrogen bonding interactions and hydrophobic inclusion interactions, which allow for the SBE-β-CD-drug complexation with strong stereoselectivity and stability. The resolution also relates to the number and location of N atoms in the enantiomers. This method will be applicable to the isolation of various types of biologically imoortant enantiomers containing N-alkyl groups.

关键词： Chiral separation Chiral mobile phase additive (CMPA)SBE-β-CDN-Alkyl group

Formation of ZnSe magic-size clusters displaying optical absorption doublets from prenucleation clusters

在线全文

学校读者我要写书评

暂无评论

Nano Research 2024年第7期17卷 6741-6748页

作者： Dingyu Zhao Shasha Wang Jiawei Xue Chunchun zhang Shanling Wang Xiaoqin Chen Chaoran Luan kui yu Institute of Atomic and Molecular Physics Sichuan UniversityChengdu 610065China Engineering Research Center in Biomaterials Sichuan UniversityChengdu 610065China Analytical&Testing Center Sichuan UniversityChengdu 610065China College of Biomedical Engineering Sichuan UniversityChengdu 610065China

The formation pathway of colloidal semiconductor ZnSe magic-size clusters(MSCs)in a reaction that display an optical absorption doublet remains poorly *** reaction of Zn(OAc)_(2)/OLA(made from zinc acetate and oleylamine)and tri-noctylphosphine selenide(SeTOP)in OLA in the presence of diphenylphosphine(HPPh_(2))is studied,in which dMSC-345 displays a doublet peaking at 328/345 *** suggest that the development is from the clusters that form in the initial prenucleation stage of the *** clusters are the precursor compound(PC-299)of MSC-299(displaying an absorption singlet peaking at 299 nm).PC-299 transforms to PC-345 at a later *** presence of alcohol(such as methanol or ethylene glycol)promotes another pathway,which is the PC-299 to PC-320 ***-320 transforms to dMSC-320(with a doublet at 305/320 nm),followed by dMSC-345 via *** present study provides additional evidence that clusters(PC-299)form and transform(such as to dMSC-345 via PC-345)in the prenucleation stage of ZnSe quantum dots(QDs).

关键词： magic-size cluster(MSC) prenucleation cluster precursor compound(PC) quantum dot(QD) zinc selenide(ZnSe)

Controllable synthesis of one-dimensional silicon nanostructures based on the dual effects of electro-deoxidation and the Kirkendall effect

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Nano Research 2024年第9期17卷 7814-7823页

作者： Jianxin Tu Shuo yu kui Hao Le Sun Ruicheng Bai Fangzhou zhang Aijun Li Hong Liu Institute of Materials School of Materials Science and EngineeringShanghai UniversityShanghai 200444China Department of Applied Physics The Hong Kong Polytechnic UniversityKowloon 999077Hong KongChina Shaoxing institute of technology Shanghai UniversityShanghai 200444China

In this study,we successfully synthesized silicon nanotubes(Si-NTs)and silicon nanowires(Si-NWs)in a controllable manner using a catalyst-and template-free method through the direct electrolysis of SiO_(2)in a molten CaCl_(2)-CaO system,while also proposing a novel formation mechanism for ***-NWs are formed through electro-deoxidation when the cell voltage is within the range of CaO decomposition voltage and SiO_(2)decomposition *** subsequently adjusting the voltage to a value between the decomposition potentials of CaCl_(2)and CaO,in-situ electro-deoxidation of CaO takes place on the surface of the synthesized Si-NWs,leading to the formation of a Ca *** formation of Ca-Si diffusion couple leads to the creation of vacancies within the Si-NWs,as the outward diffusion rate of Si exceeds the inward diffusion rate of *** differential diffusion rates between Si and Ca in a diffusion couple exhibit an analogy to the Kirkendall *** vacancies gradually accumulate and merge,forming large voids,which ultimately result in the formation of hollow *** a subsequent dealloying process,the removal of the embedded calcium leads to the formation of *** the application of a carbon coating,the Si-NTs@C composite showcases a high initial discharge capacity of 3211 mAh·g^(-1)at 1.5 A·g^(-1)and exhibits exceptional long-term cycling stability,maintaining a capacity of 977 mAh·g^(-1)after 2000 cycles at 3.0 A·g^(-1).

关键词： silicon nanotubes silicon nanowires lithium-ion battery Kirkendall effect

Recovery of cyanophycin granule polypeptide from activated sludge: carbon source dependence and aggregation-induced luminescence characteristics

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Frontiers of Environmental Science & Engineering 2024年第2期18卷 25-34页

作者： kui Zou Hongyuan Liu Bo Feng Taiping Qing Peng zhang College of Environment and Resources Xiangtan UniversityXiangtan 411105China

In the sewage treatment process,facilitating the conversion of pollutants into value-added resources holds great potential for reducing the amount of greenhouse gas emissions and promoting economic *** granule polypeptide(CGP),a recently discovered high value-added biopolymer present in activated sludge,has provided new avenues for the recovery of ***,the mechanisms that regulate CGP synthesis and the characteristics of this biopolymer in activated sludge remain unclear thus *** study investigated the synthesis of CGP,polyhydroxyalkanoates(PHA),and alginate-like exopolysaccharides(ALE)in various microbial aggregates under different carbon sources feeding *** results showed that the CGP yields was superior that of PHA and ALE when subjected to identical carbon source feeding ***,biofilm was more conducive to CGP accumulation than floc *** with glucose and methanol,sodium acetate significantly enriched the CGP synthetase-encoding gene(cphAabundance=~17419),resulting in the highest CGP yield(average 107.1 mg/g MLSS)in both biofilm and floc *** study is the first to reported the characteristic fluorescence of CGP(Ex/Em=~360/450 nm)caused by the aggregated luminescence of arginine on the side ***,this study highlights the potential application of CGP as a fluorescent material and offers insights into CGP recovery from activated sludge in wastewater treatment plants.

关键词： Cyanophycin Polyhydroxyalkanoate Alginate-like exopolysaccharides Bioresource recovery Aggregation-induced luminescence

Amorphous Iridium Oxide-Integrated Anode Electrodes with Ultrahigh Material Utilization for Hydrogen Production at Industrial Current Densities

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Nano-Micro Letters 2024年第10期16卷 225-239页

作者： Lei Ding kui Li Weitian Wang Zhiqiang Xie Shule yu Haoran yu David ACullen Alex Keane Kathy Ayers Christopher BCapuano Fangyuan Liu Pu-Xian Gao Feng-yuan zhang Department of Mechanical Aerospace and Biomedical EngineeringUniversity of TennesseeKnoxvilleTN 37996USA Oak Ridge National Lab Center for Nanophase Materials SciencesOak RidgeTN 37831USA Nel Hydrogen WallingfordCT 06492USA Institute of Materials Science University of ConnecticutStorrsCT 06269USA Department of Materials Science and Engineering University of ConnecticutStorrsCT 06269USA

Herein,ionomer-free amorphous iridium oxide(IrO_(x))thin electrodes are first developed as highly active anodes for proton exchange membrane electrolyzer cells(PEMECs)via low-cost,environmentally friendly,and easily scalable electrodeposition at room *** with a Nafion 117 membrane,the IrO_(x)-integrated electrode with an ultralow loading of 0.075 mg cm^(-2)delivers a high cell efficiency of about 90%,achieving more than 96%catalyst savings and 42-fold higher catalyst utilization compared to commercial catalyst-coated membrane(2 mg cm^(-2)).Additionally,the IrO_(x)electrode demonstrates superior performance,higher catalyst utilization and significantly simplified fabrication with easy scalability compared with the most previously reported ***,the remarkable performance could be mainly due to the amorphous phase property,sufficient Ir^(3+)content,and rich surface hydroxide groups in ***,due to the high activity,high cell efficiency,an economical,greatly simplified and easily scalable fabrication process,and ultrahigh material utilization,the IrO_(x)electrode shows great potential to be applied in industry and accelerates the commercialization of PEMECs and renewable energy evolution.

关键词： Ionomer-free Amorphous IrOx electrodes Ultrahigh material utilization Scalable electrodeposition Hydrogen production