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World Journal of Gastroenterology 2008年第26期14卷 4227-4233页

作者： Ming-yu Lai jie-An huang Zhi-Hai Liang Hai-Xing Jiang Guo-Du Tang Department of Gastroenterology First Affiliated Hospital Guangxi Medical University Nanning 530021Guangxi Zhuang Autonomous Region China

AIM： To investigate the effects of aspirin （acetylsalicylic acid） on proliferation and apoptosis of colorectal cancer cell line SW480 and its mechanism. METHODS： Cyclooxygenase （COX）-2 negative colorectal cancer cell line SW480 was treated with aspirin at concentrations of 2.5 mmol/L, 5.0 mmol/L, 10.0 mmol/L for different periods in v/tro. Anti-proliferation effect of aspirin on SW480 was detected by 3-（4,5-dimeth- ylthiazol-2-yl）-2,5-diphenyltetrazolium bromide （MTT） assay. Cell cycle and apoptosis were observed by flow cytometry （FCM）. Transmission electron microscope （TEM） was used for morphological study. Apoptosis-associated genes were detected by immunohistochemical staining and Western blotting. RESULTS： Aspirin inhibited SW480 proliferation and induced apoptosis in a dose- and time-dependent manner. Treatment with different concentrations of aspirin significantly increased the proportions of cells at the G0/G1 phase and decreased the proportions of cells at the S- and G2/M phases in a concentration-dependent manner. Aspirin not only induced apoptosis but also caused cell necrosis at a high concentration as well. After treatment with aspirin, SW480 cells displayed typically morphological features of apoptosis and necrosis under TEM, and increased the Bcl-2 expression in cells, but the expression of Bax was down regulated. CONCLUSION： Aspirin inhibits proliferation and induces apoptosis of SW480 cells. Its anti-tumor mechanism may arrest cell cycle and shift Bax/Bcl-2 balance in cells.

关键词： Aspirin Colorectal cancer Proliferation Apoptosis

A novel dimeric procyanidin glucoside from Polygonum aviculare

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Chinese Chemical Letters 2012年第7期23卷 820-822页

作者： Hai Jian Cong Shu Wei Zhang Chong Zhang yu jie huang Li Jiang Xuan State Key Laboratory of Drug Research Shanghai Institute of Materia MedicaShanghai Institute for Biological SciencesChinese Academy of SciencesShanghai 201203China

A novel dimeric procyanidin glucoside,catechin 3-O-acetate-（4 α→8）-catechin 3-O-acetate-3＇-O- β-D-glucopyranoside（1）, along with five flavonoids and one lignan were isolated from the aerial parts of Polygonum ... 详细信息

关键词： Polygonum aviculare Polygonaceae Procyanidin glucoside Catechin

Glycolytic and fatty acid oxidation genes affect the treatment and prognosis of liver cancer

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World Journal of Clinical Cases 2022年第15期10卷 4737-4760页

作者： Jia-yue Zou yu-jie huang Jun He Zu-Xiong Tang Lei Qin Department of General Surgery The First Affiliated Hospital of Soochow UniversitySuzhou 215006Jiangsu ProvinceChina

BACKGROUND Metabolic reprogramming is a feature of tumour cells and is essential to support their rapid *** glycolytic activity of liver cancer cells is significantly higher than that of normal liver cells,and the rapidly proliferating tumour cells are powered by aerobic *** metabolism reprogramming enables tumour cells to meet their needs for highly proliferative growth and is an important driving force for the development of hepatocellular carcinoma(HCC).AIM To explore the influence of different metabolic subtypes of HCC and analyse their significance in guiding prognosis and treatment based on the molecular mechanism of glycolysis and fatty acid oxidation(FAO).METHODS By downloading related data from public databases including the Cancer Genome Atlas(TCGA),the Molecular Signatures Database,and International Cancer Genome Consortium,we utilised unsupervised consensus clustering to divide TCGA Liver Hepatocellular Carcinoma samples into four metabolic subgroups and compared single nucleotide polymorphism,copy number variation,tumour microenvironment,and Genomics of Drug Sensitivity in Cancer and Tumour Immune Dysfunction and Exclusion between different *** differences and causes of survival and the clinical characteristics between them were analysed,and a prognostic model was established based on glycolysis and FAO *** with the clinical features,a Norman diagram was created to compare the pros and cons of each *** In the four metabolic subgroups,with the increase in glycolytic expression,the median survival of patients showed the worst results,while FAO showed the *** comparing the follow-up analysis of each group,we considered that the differences between them might be related to reactive oxygen species,somatic copy number variation of key genes,and immune *** was also found that the FAO group and the low-risk group had better efficacy and response to immune checkpoint blockade treat

关键词： Metabolic reprogramming Fatty acid oxidation Tumour immune dysfunction and exclusion Hepatocellular carcinoma Nomogram

Why MUC16 mutations lead to a better prognosis:A study based on The Cancer Genome Atlas gastric cancer cohort

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World Journal of Clinical Cases 2021年第17期9卷 4143-4158页

作者： yu-jie huang Zhi-Fei Cao jie Wang Jian Yang Yi-Jun Wei yu-Chen Tang Yin-Xiang Cheng Jian Zhou Zi-Xiang Zhang Department of General Surgery The First Affiliated Hospital of Soochow UniversitySuzhou 215006Jiangsu ProvinceChina Department of Pathology The Second Affiliated Hospital of Soochow UniversitySuzhou 215004Jiangsu ProvinceChina

BACKGROUND MUC16,encoding cancer antigen 125,is a frequently mutated gene in gastric *** addition,MUC16 mutations seem to result in a better prognosis in gastric ***,the mechanisms that lead to a better prognosis by MUC16 mutations have not yet been *** To delve deeper into the underlying mechanisms that explain why MUC16 mutations signal a better prognosis in gastric *** We used multi-omics data,including mRNA,simple nucleotide variation,copy number variation and methylation data from The Cancer Genome Atlas,to explore the relationship between MUC16 mutations and *** regression and random survival forest algorithms were applied to search for hub *** set enrichment analysis was used to elucidate the molecular ***-sample gene set enrichment analysis and“EpiDISH”were used to assess immune cells infiltration,and“ESTIMATE”for analysis of the tumor *** Our study found that compared to the wild-type group,the mutation group had a better *** analysis indicated that the MUC16 mutations appear to activate the DNA repair and p53 pathways to act as an anti-tumor *** also identified a key gene,NPY1R(neuropeptide Y receptor Y1),which was significantly more highly expressed in the MUC16 mutations group than in the MUC16 wild-type *** high expression of NPY1R predicted a poorer prognosis,which was also confirmed in a separate Gene Expression Omnibus *** susceptibility analysis revealed that NPY1R might be a potential drug target for gastric ***,in the analysis of the tumor microenvironment,we found that immune cells in the mutation group exhibited higher anti-tumor *** addition,the tumor mutation burden and cancer stem cells index were also higher in the mutation group than in the wild-type *** We speculated that the MUC16 mutations might activate the p53 pathway and DNA repair pathway:alternatively,the tumor microenvir

关键词： Gastric cancer MUC16 mutation Cancer antigen 125 Prognosis The Cancer Genome Atlas Gene Expression Omnibus

The First Two Moments of Aggregate Claims in a Markovian Environment

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Journal of Mathematical Research and Exposition 2010年第6期30卷 967-975页

作者： yu jie huang Jing Hai FENG Li Xin SONG Li Xin SONG1 1.School of Mathematical Science Dalian University of TechnologyLiaoning 116024P.R.China Department of Mathematics Anshan Normal UniversityLiaoning 114005P.R.China

We consider the discounted aggregate claims when the insurance risks and financial risks are governed by a discrete-time Markovian *** assume that the claim sizes and the financial risks fluctuate over time according to the states of economy,which are interpreted as the states of Markovian *** will then determine a system of differential equations for the Laplace-Stieltjes transform of the distribution of discounted aggregate claims under mild ***,using the differentio-integral equation,we will also investigate the first two order moments of discounted aggregate claims in a Markovian environment.

关键词： discrete-time insurance risk model discounted aggregate claims circumstance process moment.

Relationship Between Physiological-biochemical Changes Under Drought Stress and Drought Resistance in Ramie

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Agricultural Sciences in China 2001年第1期 83-88页

作者： jie yu-cheng huang Pi-sheng LI Zong-dao Institute of Bast Crops Chinese Academy of Agricultural Sciences Changsha 410006 Nanjing Agricultural University Nanjing 210095 Ramie Institute of Hunan Agricultural University Changsha 410128

A study on the physiological-biochemical changes of different ramie(Boehmeria Jacq. ) varieties under drought stress was carried out, the results showed that relative water contents(RWC) decreased and RPP increased with the increase of drought stress. Compared with drought sensitive varieties, drought resistant varieties had higher RWC in leaves but lower RPP. Peroxidase activity of drought-resistance varieties changed from low to high whereas sensitive ones changed in opposite direction. Proline contents of drought sensitive varieties was higher than those of drought resistant ones under a certain degree drought stress. Proline accumulation in drought resistant varieties was faster than that in drought sensitive ones under serious drought *** contents in leaf was increased under drought stress. MDA contents increased slowly in resistant varieties while that increased rapidly in sensitive ones.

关键词： Ramie Drought resistance Proline Peroxidase Relative water contents MDA

Simulating,adjusting and testing regional sustainable development──a case study of Jiangxi Province

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Journal of Geographical Sciences 2000年第2期10卷 185-192页

作者： huang yu-jie, huang yu-xia (Institute of Remote Sensing Application, Chinese Academy of Sciences, Beijing 100101, China)

Based on impact of human activities on regional information system, this paper focuses on sedating, adjusting and testing methodology adopted for the regional sustainable development. The case study of Jiangxi Provinc... 详细信息

关键词： regional sustainable development sedation adjusting plans, Jiangxi

Novel model combining contrast-enhanced ultrasound with serology predicts hepatocellular carcinoma recurrence after hepatectomy

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World Journal of Clinical Cases 2021年第24期9卷 7009-7021页

作者： Hai-Bin Tu Li-Hong Chen yu-jie huang Si-Yi Feng Jian-Ling Lin Yong-Yi Zeng Department of Ultrasound Mengchao Hepatobiliary Hospital of Fujian Medical UniversityFuzhou 350025Fujian ProvinceChina Department of Hepatobiliary Surgery Mengchao Hepatobiliary Hospital of Fujian Medical UniversityFuzhou 350025Fujian ProvinceChina

BACKGROUND Surgery is the primary curative option in patients with hepatocellular carcinoma(HCC).However,recurrence within 2 years is observed in 30%–50%of patients,being a major cause of *** To construct and verify a non-invasive prediction model combining contrastenhanced ultrasound(CEUS)with serology biomarkers to predict the early recurrence of *** Records of 744 consecutive patients undergoing first-line curative surgery for HCC in one institution from 2016–2018 were reviewed,and 292 local patients were selected for *** characteristics including gender and age,CEUS liver imaging reporting and data system(LIRADS)parameters including wash-in time,wash-in type,wash-out time,and wash-out type,and serology biomarkers including alanine aminotransferase,aspartate aminotransferase,platelets,and alpha-fetoprotein(AFP)were *** analysis and multivariate Cox proportional hazards regression model were used to evaluate the independent prognostic factors for tumor *** a nomogram called CEUS model was *** CEUS model was then used to predict recurrence at 6 mo,12 mo,and 24 mo,the cut-off value was calculate by X-tile,and each C-index was *** Kaplan-Meier curve was compared by logrank *** calibration curves of each time were *** A nomogram predicting early recurrence(ER),named CEUS model,was formulated based on the results of the multivariate Cox regression *** nomogram incorporated tumor diameter,preoperative AFP level,and LIRADS,and the hazard ratio was 1.123(95%confidence interval[CI]:1.041-1.211),1.547(95%CI:1.245-1.922),and 1.428(95%CI:1.059-1.925),*** cut-off value at 6 mo,12 mo,and 24 mo was 100,80,and 50,and the C-index was 0.748(95%CI:0.683-0.813),0.762(95%CI:0.704-0.820),and 0.762(95%CI:0.706-0.819),*** model showed satisfactory results,and the calibration at 6 mo was desirable;however,the calibration at 12 and 24 mo should be improv

关键词： Hepatocellular carcinoma Recurrence Prediction Contrast-enhanced ultrasound Liver imaging reporting and data system Alpha-fetoprotein

N6-methyladenosine methylation regulates the tumor microenvironment of Epstein-Barr virus-associated gastric cancer

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World Journal of Gastrointestinal Oncology 2024年第6期16卷 2555-2570页

作者： yu Zhang Fang Zhou Ming-yu Zhang Li-Na Feng Jia-Lun Guan Ruo-Nan Dong yu-jie huang Su-Hong Xia Jia-Zhi Liao Kai Zhao Department of Gastroenterology Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030Hubei ProvinceChina Institute of Liver and Gastrointestinal Diseases Hubei Key Laboratory of Hepato-Pancreato-Biliary DiseasesTongji Hospital of Tongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030Hubei ProvinceChina Department of Pharmacy Wuhan Fourth HospitalWuhan 430030Hubei ProvinceChina

BACKGROUND N6-methyladenosine(m6A)methylation modification exists in Epstein-Barr virus(EBV)primary infection,latency,and lytic *** also modifies EBV latent genes and lytic ***-associated gastric cancer(EBVaGC)is a distinctive molecular subtype of *** hypothesized EBV and m6A methylation regulators interact with each other in EBVaGC to differentiate it from other types of *** To investigate the mechanisms of m6A methylation regulators in EBVaGC to determine the differentiating factors from other types of *** First,The Cancer Gene Atlas and Gene Expression Omnibus databases were used to analyze the expression pattern of m6A methylation regulators between EBVaGC and EBV-negative GC(EBVnGC).Second,we identified Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)functional enrichment of m6A-related differentially expressed *** quantified the relative abundance of immune cells and inflammatory factors in the tumor microenvironment(TME).Finally,cell counting kit-8 cell proliferation test,transwell test,and flow cytometry were used to verify the effect of insulin-like growth factor binding protein 1(IGFBP1)in EBVaGC cell *** m6A methylation regulators were involved in the occurrence and development of *** with EBVnGC,the expression levels of m6A methylation regulators Wilms tumor 1-associated protein,RNA binding motif protein 15B,CBL proto-oncogene like 1,leucine rich pentatricopeptide repeat containing,heterogeneous nuclear ribonucleoprotein A2B1,IGFBP1,and insulin-like growth factor 2 binding protein 1 were significantly downregulated in EBVaGC(P<0.05).The overall survival rate of EBVaGC patients with a lower expression level of IGFBP1 was significantly higher(P=0.046).GO and KEGG functional enrichment analyses showed that the immunity pathways were significantly activated and rich in immune cell infiltration in *** with EBVnGC,the infiltration of activated CD4+T cells,activated CD8+T cel

关键词： N6-methyladenosine methylation Tumor microenvironment Epstein-Barr virus Gastric cancer Insulin-like growth factor binding protein 1

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氧化钙对流化床煤温和气化半焦性质的影响

燃料化学学报 2000年第1期28卷 40-43页

作者：朱廷钰张守玉黄戒介王洋中国科学院山西煤炭化学研究所太原030001

在Φ4 8× 5 2 5mm小型流化床反应器中 ,于 4 5 0～ 75 0℃温度区间内 ,研究了氧化钙对煤温和气化产物半焦特性的影响。报道了在煤温和气化过程中添加氧化钙对所得半焦的工业分析、元素组成及热值的影响。采用等温吸附法研究了氧化钙对... 详细信息

在Φ4 8× 5 2 5mm小型流化床反应器中 ,于 4 5 0～ 75 0℃温度区间内 ,研究了氧化钙对煤温和气化产物半焦特性的影响。报道了在煤温和气化过程中添加氧化钙对所得半焦的工业分析、元素组成及热值的影响。采用等温吸附法研究了氧化钙对半焦孔结构和比表面积的影响 ,并给予了合理的解释。

关键词：温和气化流化床半焦微观特性气化氧化钙