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A novel oncolytic adenovirus inhibits hepatocellular carcinoma growth

Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2019年第12期20卷 1003-1013页

作者： yu-huan bai Xiao-jing yuN Yan XUE Ting ZHOU Xin SUN Yan-jing GAO Department of Gastroenterology Qilu Hospital of Shandong UniversityJinan 250012China Department of Gastroenterology the Second People's Hospital of LiaochengLinqing 252600China

Objective: To evaluate the inhibitory role of a novel oncolytic adenovirus(OA), GP73-SphK1 sR-Ad5, on the growth of hepatocellular carcinoma(HCC). Methods: GP73-SphK1 sR-Ad5 was constructed by integrating Golgi protein 73(GP73) promoter and sphingosine kinase 1(SphK1)-short hairpin RNA(shRNA) into adenovirus serotype 5(Ad5), and transfecting into HCC Huh7 cells and normal human liver HL-7702 cells. The expression of SphK1 and adenovirus early region 1(E1 A) was detected by quantitative real-time PCR(qRT-PCR) and western blot, respectively. Cell viability was detected by methylthiazolyldiphenyl-tetrazolium bromide(MTT) assay, and apoptotic rate was determined by flow cytometry. An Huh7 xenograft model was established in mice injected intratumorally with GP73-SphK 1 sR-Ad5. Twenty days after injection, the tumor volume and weight, and the survival time of the mice were recorded. The histopathological changes in tumor tissues were observed by hematoxylin-eosin(HE) staining and transmission electron microscopy(TEM). Results: Transfection of GP73-SphK1 sR-Ad5 significantly upregulated E1 A and downregulated SphK1 in Huh7 cells, but not in HL7702 cells. GP73-SphK1 sR-Ad5 transfection significantly decreased the viability and increased the apoptotic rate of Huh7 cells, but had no effect on HL7702 cells. Intratumoral injection of GP73-SphK1 sR-Ad5 into the Huh7 xenograft mouse model significantly decreased tumor volume and weight, and prolonged survival time. It also significantly decreased the tumor infiltration area and blood vessel density, and increased the percentages of cells with nucleus deformation and cells with condensed chromatin in tumor tissues. Conclusions: GP73-SphK1 sR-Ad5 serves as a novel OA and can inhibit HCC progression with high specificity and efficacy.

关键词： Hepatocellular carcinoma Oncolytic adenovirus Golgi protein 73 Sphingosine kinase 1

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Experimental Study on Towing Characteristics of Composite Bucket Wellhead Platform

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China Ocean Engineering 2024年第3期38卷 531-542页

作者： ZHANG Pu-yang LIU Ying-fei LE Cong-huan DING Hong-yan bai yu State Key Laboratory of Hydraulic Engineering Intelligent Construction and Operation Tianjin UniversityTianjin 300072China

With the rapid development of large-scale development of marginal oilfields in China,simple wellhead platforms that are simple in structure and easy to install have become an inevitable choice in the process of oilfield ***,traditional simple wellhead platforms are often discarded after a single *** pursuit of a more costeffective approach to developing marginal oilfields,this paper proposes a new offshore oil field development facility—an integrated bucket foundation for wellhead *** verify the safety of its towing behavior and obtain the dynamic response characteristics of the structure,this paper takes a bucket integrated bucket foundation for wellhead platform with a diameter of 40 m as the research *** combining physical model tests and numerical simulations,it analyzes the static stability and dynamic response characteristics of the structure during towing,complete with the effects of the draft,wave height,wave period,and towing point height,which produce the dynamic responses of the structure under different influence factors,such as roll angle,pitch angle,heave acceleration and towing force as well as the sensibility to transport *** results show that the integrated bucket foundation for wellhead platform is capable of self-floating towing,and its movement is affected by the local environment,which will provide a reference for actual projects.

关键词： composite bucket foundation wellhead platform air flotation towing model test

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A Novel Analysis Approach of Uniform Persistence for an Epidemic Model with Quarantine and Standard Incidence Rate

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Acta Mathematicae Applicatae Sinica 2024年第3期40卷 695-707页

作者： Song-bai GUO yu-ling XUE Xi-liang LI Zuo-huan ZHENG School of Science Beijing University of Civil Engineering and ArchitectureBeijing 102616China School of Mathematics and Statistics Hainan Normal UniversityHaikou 571158China Academy of Mathematics and Systems Science Chinese Academy of SciencesBeijing 100190China School of Mathematics and Information Science Shandong Technology and Business UniversityYantai 264005China School of Mathematical Sciences University of Chinese Academy of SciencesBeijing 100049China

Inspired by the transmission characteristics of the coronavirus disease 2019(COVID-19),an epidemic model with quarantine and standard incidence rate is first developed,then a novel analysis approach is proposed for finding the ultimate lower bound of the number of infected individuals,which means that the epidemic is uniformly persistent if the control reproduction number R_(c)>*** approach can be applied to the related biomat hem at ical models,and some existing works can be improved by using *** addition,the infection-free equilibrium V^(0)of the model is locally asymptotically stable(LAS)if R_(c)1.

关键词： uniform persistence epidemic model control reproduction number quarantine standard incidence rate

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Proteinase-activated receptor 2 promotes tumor cell proliferation and metastasis by inducing epithelialmesenchymal transition and predicts poor prognosis in hepatocellular carcinoma

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World Journal of Gastroenterology 2018年第10期24卷 1120-1133页

作者： Liang Sun Pi-Bao Li Yan-Fen Yao Ai-yuan Xiu Zhi Peng yu-huan bai Yan-Jing Gao Qilu Hospital of Shandong University Jinan 250012Shandong ProvinceChina Department of Critical Care Medicine Shandong Traffic HospitalJinan 250000Shandong provinceChina Department of Gastroenterology Qilu Hospital of Shandong UniversityJinan 250012Shandong provinceChina

AIM To clarify the role of proteinase-activated receptor 2(PAR2) in hepatocellular carcinoma, especially in the process of *** PAR2 expression levels were assessed by qRT-PCR and immunohistochemistry(IHC) in patient tissues and in hepatocellular carcinoma cell lines SMMC-7721 and Hep G2. Cell proliferation and metastasis were assessed both in vitro and in vitro. Immunoblotting was carried out to monitor the levels of mitogen-activated protein kinase(MAPK) and epithelial-mesenchymal transition *** The prognosis was significantly poorer in patients with high PAR2 levels than in those with low PAR2 levels. Patients with high PAR2 levels had advanced tumor stage(P = 0.001, chi-square test), larger tumor size(P = 0.032, chi-square test), and high microvascular invasion rate(P = 0.037, chi-square test). The proliferation and metastasis ability of SMMC-7721 and Hep G2 cells was increased after PAR2 overexpression, while knockdown of PAR2 decreased the proliferation and metastasis ability of SMMC-7721 and Hep G2 cells. Knockdown of PAR2 also inhibited hepatocellular carcinoma tumor cell growth and liver metastasis in nude mice. Mechanistically, PAR2 increased the proliferation ability of SMMC-7721 and Hep G2 cells via ERK activation. Activated ERK further promoted the epithelial-mesenchymal transition of these cells, which endowed them with enhanced migration and invasion ability. CONCLUSION These data suggest that PAR2 plays an important role in the proliferation and metastasis of hepatocellular carcinoma. Therefore, targeting PAR2 may present a favorable target for treatment of this malignancy.

关键词： Hepatocellular carcinoma Proteinaseactivated receptor 2 Epithelial-mesenchymal transition

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Identity-based searchable attribute signcryption in lattice for a blockchain-based medical system

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Frontiers of Information Technology & Electronic Engineering 2024年第3期25卷 461-471页

作者： Huifang yu Xiaoping bai School of Cyberspace Security Xi'an University of Posts&TelecommunicationsXi'an 710121China School of Information Engineering Qinghai Communications Technical CollegeXining 810003China

Electronic healthcare systems can offer convenience but face the risk of data forgery and information *** solve these issues,we propose an identity-based searchable attribute signcryption in lattice for a blockchain-based medical system(BCMS-LIDSASC).BCMS-LIDSASC achieves decentralization and anti-quantum security in the blockchain environment,and provides fine-grained access control and ***,smart contracts are used to replace traditional trusted third parties,and the interplanetary file system(IPFS)is used for ciphertext storage to alleviate storage pressure on the *** to other schemes,BCMS-LIDSASC requires smaller key size and less storage,and has lower computation *** contributes to secure and efficient management of medical data and can protect patient privacy and ensure the integrity of electronic healthcare systems.

关键词： Blockchain Identity-based searchable attribute signcryption Distributed storage NTRU lattice

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Atmospheric chemistry of 2-nitrobenzaldehyde:Initiated by photo-excitation,OH-oxidation,and small TiO_(2) clusters adsorption catalysis

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Journal of Environmental Sciences 2024年第5期139卷 123-137页

作者： Feng-Yang bai Xiang-huan Liu Ting-Ting Meng Zhou yu Yi-Chen Wang Shuang Ni Zhen Zhao Institute of Catalysis for Energy and Environment College of Chemistry and Chemical EngineeringShenyang Normal UniversityShenyang 110034China

The fate of 2-nitrobenzaldehyde(2-NBA)is of interest in atmospheric chemistry as it is a semi-volatile organic compound with high *** study presents a quantum chemical study of the gas-phase reactions of 2-NBA photo-excitation and OH-oxidation in the absence and presence of small TiO_(2) *** further understand the unknown photolysis mechanism,the photo-reaction pathways of ground singlet state and the lying excited triplet state of 2-NBA were investigated including the initial and subsequent reactions of proton transfer,direct CO,NO_(2),and HCO elimination routes in the presence of O_(2) and ***,the OH-mediated degradation of 2-NBA proceeded via five H-extraction and six OH-addition channels by indirect mechanism,which follows a succession of reaction steps initiated by the formation of weakly stable intermediate *** H-extraction from the-CHO group was the dominant pathway with a negative activation energy of-1.22 kcal/*** calculated rate coefficients at 200–600 K were close to the experimental data in literature within 308-352 K,and the kinetic negative temperature independence was found in both experimental literature and computational ***,2-NBA was favored to be captured onto small TiO_(2) clusters via six adsorption configurations formed via various combination of three types of bonds of Ti…O,Ti…C,and O…H between the molecularly adsorbed 2-NBA and TiO_(2) *** indicted that the chemisorptions of aldehyde oxygen have largest *** results suggested adsorption conformations have a respectable impact on the catalysis *** study is significant for understanding the atmospheric chemistry of 2-nitrobenzaldehyde.

关键词： 2-nitrobenzaldehyde OH radical oxidation Rate coefficient Photolysis mechanism Adsorption catalysis

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Barley Protein LFBEP-C1 from Lactiplantibacillus plantarum dy-1 Fermented Barley Extracts by Inhibiting Lipid Accumulation in a Caenorhabditis elegans Model

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Biomedical and Environmental Sciences 2024年第4期37卷 377-386页

作者： ZHANG Jia Yan LIU Meng Ting LIU yu Hao DENG huan bai Juan XIE Jian Hua XIAO Xiang School of Food and Biological Engineering Jiangsu UniversityZhenjiang 212013JiangsuChina State Key Laboratory of Food Science and Technology Nanchang UniversityNanchang 330047JiangxiChina

Objective This study aimed to investigate the lipid-lowering activity of LFBEP-C1 in high glucose-fed Caenorhabditis elegans(***).Methods In this study,the fermented barley protein LFBEP-C1 was prepared and tested for its potential anti-obesity effects on *** worms were fed Escherichia coli OP50(*** OP50),glucose,and different concentrations of *** size,lifespan,movement,triglyceride content,and gene expression were *** results were analyzed using ANOVA and Tukey's multiple comparison *** Compared with the model group,the head-swing frequency of *** in the group of LFBEP-C1 at 20μg/mL increased by 33.88%,and the body-bending frequency increased by 27.09%.This indicated that LFBEP-C1 improved the locomotive ability of *** average lifespan of *** reached 13.55 days,and the body length and width of the *** decreased after LFBEP-C1 ***,LFBEP-C1 reduced the content of lipid accumulation and triglyceride *** expression levels of sbp-1,daf-2,and mdt-15 significantly decreased,while those of daf-16,tph-1,mod-1,and ser-4 significantly increased after LFBEP-C1 *** in these genes explain the signaling pathways that regulate lipid *** LFBEP-C1 significantly reduced lipid deposition in *** fed a high-glucose diet and alleviated the adverse effects of a high-glucose diet on the development,lifespan,and exercise behavior of *** addition,LFBEP-C1 regulated lipid metabolism mainly by mediating the expression of genes in the sterol regulatory element-binding protein,insulin,and 5-hydroxytryptamine signaling pathways.

关键词： LFBEP-C1 Fermentation Protein Caenorhabditis elegans Lipid accumulation Signaling pathway

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Supramolecular Biomaterials for Cancer Immunotherapy

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Research 2024年第2期2023卷 491-509页

作者： huan Liang Qingqing Lu Jie Yang Guocan yu College of Science Nanjing Forestry UniversityNanjing 210037P.R.China Key Laboratory of Bioorganic Phosphorus Chemistry&Chemical Biology Department of ChemistryTsinghua UniversityBeijing 100084P.R.China

Cancer immunotherapy has achieved tremendous successful clinical results and obtained historic victories in tumor ***,great limitations associated with feeble immune responses and serious adverse effects still cannot be neglected due to the complicated multifactorial etiology and pathologic microenvironment in *** rapid development of nanomedical science and material science has facilitated the advanced progress of engineering biomaterials to tackle critical *** supramolecular biomaterials with flexible and modular structures have exhibited unparalleled advantages of high cargo-loading efficiency,excellent biocompatibility,and diversiform immunomodulatory activity,thereby providing a powerful weapon for cancer *** past decades,supramolecular biomaterials were extensively explored as versatile delivery platforms for immunotherapeutic agents or designed to interact with the key moleculars in immune system in a precise and controllable *** this review,we focused on the crucial role of supramolecular biomaterials in the modulation of pivotal steps during tumor immunotherapy,including antigen delivery and presentation,T lymphocyte activation,tumor-associated macrophage elimination and repolarization,and myeloid-derived suppressor cell *** on extensive research,we explored the current limitations and development prospects of supramolecular biomaterials in cancer immunotherapy.

关键词： biomaterials limitations thereby

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Boundary Behaviors for a Continuous-state Nonlinear Neveu’s Branching Process

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Acta Mathematica Sinica,English Series 2024年第8期40卷 2005-2016页

作者： Lin yu bai Xu YANG School of Mathematics and Information Science North Minzu UniversityYinchuan 750021P.R.China

By generalizing a criterion of Mufa Chen for Markov jump processes,we establish the necessary and sufficient conditions for the extinction,explosion and coming down from infinity of a continuous-state nonlinear Neveu... 详细信息

关键词： Continuous-state branching process nonlinear branching Neveu’s branching extinction explosion coming down from infinity

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Derivatives of Intersection Local Time for Two Independent Symmetricα-stable Processes

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Acta Mathematica Sinica,English Series 2024年第5期40卷 1273-1292页

作者： huan ZHOU Guang Jun SHEN Qian yu Department of Mathematics Anhui Normal UniversityWuhu 241002P.R.China Department of Mathematics Nanjing University of Aeronautics and AstronauticsNanjing 211106P.R.China Key Laboratory of Mathematical Modelling and High Performance Computing of Air Vehicles(NUAA) MIITNanjing 211106P.R.China

In this paper,we consider the derivatives of intersection local time for two independent d-dimensional symmetricα-stable processes X^(α) and X^(α)with respective indices α and α.We first study the sufficient condition for the existence of the derivatives,which makes us obtain the exponential integrability and H?lder *** we show that this condition is also necessary for the existence of derivatives of intersection local time at the ***,we also study the power variation of the derivatives.

关键词： Symmetric stable processes intersection local time exponential integrability power variation

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