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Journal of Pharmaceutical Analysis 2016年第2期6卷 80-86页

作者： PeiZhang yu-qiang gou Xia Gao Rui-Bin Bai Wen-Xia Chen Bo-Lu Sun Fang-Di Hu Wang-Hong Zhao School of Pharmacy Lanzhou University Lanzhou 730000 China Lanzhou Military Command Center for Disease Prevention and Control Lanzhou 730020 China Department of Stomatology Nanfang Hospital Southern Medical University Guangzhou 510000 China

An electrochemical method based on a directly electrochemically reduced graphene oxide （ERGO） film coated on a glassy carbon electrode （GCE） was developed for the rapid and convenient determination of rutin in plasma. ERGO was modified on the surface of GCE by one-step electro-deposition method. Electrochemical behavior of rutin on ERGO/GCE indicated that rutin underwent a surface-controlled quasi-reversible process and the electrochemical parameters such as charge transfer coefficient （α）, electron transfer number （n） and electrode reaction standard rate constant （ks） were 0.53, 2 and 3.4 s -1, respectively. The electrochemical sensor for rutin in plasma provided a wide linear response range of 4.70 × 10 ^-7 1.25 × 10^-5 M with the detection limit （s/n=3） of 1.84 × 10^-8 M. The assay was success- fully used to the pharmacokinetic study of rutin. The pharmacokinetic parameters such as elimination rate half-life （t1/2）, area under curve （AUC）, and plasma clearance （CL） were calculated to be 3.345 ± 0.647 rain, 5750 ±656.0 μg min/mL, and 5.891± 0.458 mL/min/kg, respectively. The proposed method utilized a small sample volume of 10 μL and had no complicated sample pretreatment （without deproteinization）, which was simple, eco-friendly, and time- and cost-efficient for rutin pharmacokinetic studies.

关键词： Electrochemically reduced graphene oxide Pharmacokinetics Rat plasma Rutin

Evaluation preparation technology of Xiaochaihu granules using fingerprint-peak pattern matching

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Journal of Pharmaceutical Analysis 2011年第2期1卷 119-124页

作者： yu-Qiong Wu yu- qiang gou Jing Han Ying-Yan Bi Shi-Lan Feng Fang-Di Hu Chun- Ming Wang School of Pharmacy Lanzhou University Lanzhou 730000 China the Second People's Hospital of Gansu Province Lanzhou 730000 China Center for Disease Control and Prevention Lanzhou Military Area Command of Chinese People's Liberation Army Lanzhou 730000 China College of Chemistry and Chemical Engineering Lanzhou University Lanzhou 730000 China

An approach was proposed to evaluate preparation technology by means of fingerprint-peak matching technology of high performance liquid chromatography with diode array detector （HPLC-DAD）. Similarity and hierarchical clustering analysis （HCA） were applied to identify the 15 batches of Xiaochaihu granules from different manufacturers and our laboratory, and peak pattern matching between the composite formulae and Radix Bupleuri Chinensis, which was one of the main ingredients of Xiaochaihu granules, was utilized to evaluate the preparation technology of Xiaochaihu granules via the indexes of the relative deviation of retention time （RT） and UV spectrum feature similarity of their corresponding peaks. Eleven matching peaks were found between Xiaochaihu granules and Radix Bupleuri Chinensis. However, the saikosaponin A and saikosaponin D, which are the important active components in Radix Bupleuri Chinensis, were not found in Xiaochaihu granules from any manufacturers. The peak areas of 11 characteristic peaks of Xiaochaihu granules samples formed a matrix of 11 × 15. The result of HCA showed that Xiaochaihu granules samples were divided into four kinds of category. Xiaochaihu granules samples from the same manufacturer were basically clustered of the same category. The results suggested that the saikosaponin A and saikosaponin D are prone to structural transformation under the condition of decoction and in the presence of the organic acidic components. These active components, existing in raw herb, might transform to a series of non-active secondary saikosaponin due to unfavourable preparation technology. So the conventional decoction-based preparation technology of Xiaochaihu granules might greatly affect its quality and therapeutic effectiveness. This study demonstrates that fingerprint-peak matching technology can not only be used for quality control of this composite formulae, but also provide some guidance for preparation technology of Xiaochaihu granules.

关键词： Xiaochaihu granules Radix Bupleuri Chinensis fingerprint-peak matching hierarchical clustering analysis

Composite-mask GAN based on refined optical flow and disparity map for SLAM visual odometry

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Optoelectronics Letters 2025年

作者： JI yuehui JIANG Jingwei LIU Junjie SONG yu GAO qiang School of Electrical Engineering and Automation， Tianjin University of Technology Tianjin Key Laboratory of Control Theory and Application for Complex Systems

Although deep learning methods have been widely applied in slam visual odometry over the past decade with impressive improvements， the accuracy remains limited in complex dynamic environments. In this paper， a composite mask-based generative adversarial network is introduced to predict camera motion and binocular depth maps. Specifically， a perceptual generator is constructed to obtain the corresponding parallax map and optical flow from between two neighboring frames. Then， an iterative pose improvement strategy is proposed to improve the accuracy of pose estimation. Finally， a composite mask is embedded in the discriminator to sense structural deformation in the synthesized virtual image， thereby increasing the overall structural constraints of the network model， improving the accuracy of camera pose estimation， and reducing drift issues in the Visual Odometer. Detailed quantitative and qualitative evaluations on the KITTI dataset show that the proposed framework outperforms existing conventional， supervised learning and unsupervised depth VO methods， providing better results in both pose estimation and depth estimation.

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De novo synthesis of atropisomeric benzofurans via Cu/SPDO complex catalyzed asymmetric formal [3 + 2] annulation

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Science China Chemistry 2025年第1期 174-180页

作者： Fan-Xiao Meng Xing-yu Wang Ka Lu Ju-Song Yang Fu-Min Zhang Ai-Jun Ma Bao Du Yong-qiang Tu State Key Laboratory of Applied Organic Chemistry College of Chemistry and Chemical Engineering Lanzhou University School of Chemistry and Chemical Engineering Shanghai Jiao Tong University School of Biotechnology and Health Sciences Wuyi University

Herein, the de novo synthesis of atropisomeric benzofurans through the Cu/spirocyclic-pyrrolidine-oxazoline(SPDO) complex catalyze asymmetric formal [3 + 2] cycloaddition between quinones and ynamides is explored, producing a range of structurally diverse axially chiral benzofurans in satisfactory yields with good to excellent enantioselectivities. Furthermore, density functional theory calculations were applied to explain the possible reaction mechanism.

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Heavy metal contamination and source in arid agricultural soil in central Gansu Province,China

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Journal of Environmental Sciences 2008年第5期20卷 607-612页

作者： LI yu gou Xin WANG Gang ZHANG qiang SU Qiong XIAO Guoju School of Chemical Engineering Northwest University for Nationalities Lanzhou 730000 China Institute of Arid Meteorology China Meteorological Administration Gansu Key Laboratory of Arid Climate Changes and Disaster Reduction Lanzhou 730020 China Key laboratory of Arid and Grassland Ecology Ministry of Education Lanzhou University Lanzhou 730000 China

Concentrations of copper （Cu）, lead （Pb）, chromium （Cr）, mercury （Hg）, and arsenic （As） were measured in arid agricultural and irrigated agricultural soils collected in Daba Village, Shajiawuan Village, Gangou Village and Sifangwu Village, located in central Gansu Province, China. Concentrations except Hg and Pb were lower than the background values in grey calcareous soil in the selected arid agricultural soils. Pb concentration exceeded the threshold of arid agricultural soils in China by 72.46%. These results showed that there was indeed serious pollution with Pb, a slight pollution problem for other selected metals in the irrigated agricultural soils in Daba Village. Principal component analysis （PCA） was used to assess the soil data, applying varimax rotation with Kaiser Normalization. The result showed that the irrigated factor, agricultural factor and anthropogenic factor all contributed to the relations between selected chemical properties. The main factor of accumulation of Cu, Pb, Cr, Hg and As was lithological factor in arid agricultural areas. There is a striking dissimilarity of origin of Cu, Pb, Cr, Hg and As in agricultural soil between the irrigate agriculture and arid agriculture.

关键词： arid agricultural area irrigate agriculture principal component analysis (PCA)

Study of Spudcan Reinstallation Near Existing Footprint in Soft Clay

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China Ocean Engineering 2022年第2期36卷 322-331页

作者： LI Sa LI Ting-ting SUN Li-qiang LIU Xin gou Le-yu School of Civil Engineering Tianjin UniversityTianjin 300350China State Key Laboratory of Hydraulic Engineering Simulation and Safety Tianjin UniversityTianjin 300350China

Spudcan extraction leaves a footprint and disturbed soil surrounding the footprint,which introduces uncertainty and complexity in the spudcan−footprint interaction during spudcan *** paper reports large deformation from finite element results of spudcan reinstallation near the footprint considering the effect of soil *** stages are proposed based on the failure mechanisms of a spudcan reinstalled in disturbed soil and undisturbed ***,the effects of soil disturbance on the position of the maximum horizontal force and moment are *** could be found that the critical offset distance in the disturbed case is larger than that in the undisturbed *** addition,the maximum horizontal force and moment occur at different depths in the disturbed case and undisturbed case.A critical area is proposed based on the analyses,which could be useful for determining the position of the maximum horizontal force and moment in practical design.

关键词： spudcan footprint reinstallation soil disturbance

AAV-mediated expression of p65shRNA and bone morphogenetic protein 4 synergistically enhances chondrocyte regeneration

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中国组织工程研究 2025年第17期29卷 3537-3547页

作者： yu Yangyi Song Zhuoyue Lian qiang Ding Kang Li Guangheng Shenzhen Key Laboratory of Musculoskeletal Tissue Reconstruction and Function Restoration Division of Adult Joint Reconstruction and Sports MedicineDepartment of Orthopedic SurgeryShenzhen People’s Hospital(The Second Clinical Medical College Jinan UniversityThe First Affiliated HospitalSouthern University of Science and Technology)Shenzhen 518020Guangdong ProvinceChina Department of Orthopedics Zhengzhou Orthopedics HospitalZhengzhou 450000Henan ProvinceChina Department of Orthopedics Shenzhen Pingle Orthopedics HospitalShenzhen 518000Guangdong ProvinceChina

BACKGROUND:Adeno-associated virus(AAV)gene therapy has been proven to be reliable and safe for the treatment of osteoarthritis in recent ***,given the complexity of osteoarthritis pathogenesis,single gene manipulation for the treatment of osteoarthritis may not produce satisfactory *** studies have shown that nuclear factorκB could promote the inflammatory pathway in osteoarthritic chondrocytes,and bone morphogenetic protein 4(BMP4)could promote cartilage ***:To test whether combined application of AAV-p65shRNA and AAV-BMP4 will yield the synergistic effect on chondrocytes regeneration and osteoarthritis ***:Viral particles containing AAV-p65-shRNA and AAV-BMP4 were *** efficacy in inhibiting inflammation in chondrocytes and promoting chondrogenesis was assessed in vitro and in vivo by transfecting AAV-p65-shRNA or AAV-BMP4 into *** experiments were divided into five groups:PBS group;osteoarthritis group;AAV-BMP4 group;AAV-p65shRNA group;and BMP4-p65shRNA 1:1 *** were collected at 4,12,and 24 weeks *** staining,including safranin O and Alcian blue,was applied after collecting articular ***,the optimal ratio between the two types of transfected viral particles was further investigated to improve the chondrogenic potential of mixed cells in *** AND CONCLUSION:The combined application of AAV-p65shRNA and AAV-BMP4 together showed a synergistic effect on cartilage regeneration and osteoarthritis *** cells transfected with AAV-p65shRNA and AAV-BMP4 at a 1:1 ratio produced the most extracellular matrix synthesis(P<0.05).In vivo results also revealed that the combination of the two viruses had the highest regenerative potential for osteoarthritic cartilage(P<0.05).In the present study,we also discovered that the combined therapy had the maximum effect when the two viruses were administered in equal *** either p65shRNA or BMP4

关键词： osteoarthritis adeno-associated virus bone morphogenetic protein 4 p65-short hairpin RNA gene therapy short hairpin RNA transforming growth factor-β1 extracellular matrix articular cartilage chondrocytes.

卡瑞利珠单抗治疗晚期转移性食管癌的临床疗效及安全性分析

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临床研究 2025年第1期33卷 65-68页

作者：俞强南阳豫西健和医院肿瘤内科河南南阳474500

目的探究转移性食管癌的晚期治疗中应用卡瑞利珠单抗的价值。方法选取南阳豫西健和医院2021年8月至2024年1月期间收入的64例转移性食管癌晚期患者,依据治疗方案的差异,将样本划分为对照组(n=32)采用常规化疗(紫杉醇+奈达铂)、而研究组(n... 详细信息

目的探究转移性食管癌的晚期治疗中应用卡瑞利珠单抗的价值。方法选取南阳豫西健和医院2021年8月至2024年1月期间收入的64例转移性食管癌晚期患者,依据治疗方案的差异,将样本划分为对照组(n=32)采用常规化疗(紫杉醇+奈达铂)、而研究组(n=32)在对照组治疗方法的基础上额外使用卡瑞利珠单抗治疗,3周为1个治疗周期,以上两组均治疗4个周期。分析两组临床疗效、免疫功能指标、免疫检查点情况、肿瘤标志物、日常生存质量及不良反应情况方面的差异。结果研究组疾病控制率高于对照组,差异具有统计学意义(P0.05),CD8^(+)T水平均低于治疗前,CD3^(+)T、CD4^(+)T/CD8^(+)T均高于治疗前,且研究组变化程度均高于对照组,差异均有统计学意义(P0.05)结论在常规化疗基础上联合应用卡瑞利珠单抗治疗转移性食管癌,能够改善患者免疫指标、免疫检查点、肿瘤标志物水平,显著提升疾病控制率,提高日常生存质量,保障用药安全性,具有临床应用意义。

关键词：卡瑞利珠单抗治疗晚期转移性食管癌临床疗效

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16层螺旋CT冠状动脉病变的诊断价值

实用放射学杂志 2006年第11期22卷 1341-1343页

作者：王西惠习羽薛红强苟晓光陈涛彭炜玲徐创社殷颜荣彭宝珠宝鸡市中心医院CT室宝鸡市中心医院CT室陕西宝鸡721008

目的评价16层螺旋CT冠状动脉造影对冠状动脉病变的诊断价值。方法对80例冠心病(76例拟冠心病患者,4例冠状动脉支架及搭桥术后患者)行多层螺旋CT(MSCT)增强扫描及后处理。结果80例中共显示斑块204处,其中硬斑块128处,软斑块96处,尤以左... 详细信息

目的评价16层螺旋CT冠状动脉造影对冠状动脉病变的诊断价值。方法对80例冠心病(76例拟冠心病患者,4例冠状动脉支架及搭桥术后患者)行多层螺旋CT(MSCT)增强扫描及后处理。结果80例中共显示斑块204处,其中硬斑块128处,软斑块96处,尤以左前降支为最多;冠状动脉狭窄共60例,其中轻度狭窄36例,中度狭窄16例,重度狭窄8例,仍以左前降支为多。心脏肿瘤3例,中纵隔囊肿1例,主动脉夹层3例,真性动脉瘤1例,大血管畸形3例。结论16层螺旋CT对冠状动脉狭窄程度,斑块性质的评价,具有较高的应用价值,是安全、准确、可靠的冠心病诊断方法。

关键词：冠状动脉疾病体层摄影术,X线计算机

CRYSTALLIZATION BEHAVIOR, THERMAL AND MECHANICAL PROPERTIES OF PHBV/GRAPHENE NANOSHEET COMPOSITES

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Chinese Journal of Polymer Science 2013年第4期31卷 670-678页

作者： Bing-jie Wang yu-jie Zhang Jian-qiang Zhang Qu-ting gou Zong-bao Wang Peng Chen 顾群 Ningbo Institute of Material Technology and Engineering Chinese Academy of Sciences School of Petrochemical Technology Lanzhou University of Technology

Biodegradable poly（3-hydroxybutyrate-co-3-hydroxyvalerate）（PHBV）/graphene nanosheet （GNS） composites were prepared via a solution-casting method at low GNS loadings in this work. Transmission electron microscopy revealed that a fine dispersion of GNSs was achieved in the PHBV matrix. The thermal properties of the nanocomposites were investigated by thermogravimetric analysis, and the results showed that the thermal stability of PHBV was significantly improved with a very low loading of GNSs. Nonisothermal melts crystallization behavior, spherulitic morphology and crystal structure of neat PHBV and the PHBV/GNSs nanocomposites were investigated, and the experimental results indicated that crystallization behavior of PHBV was enhanced by the presence of GNSs due to the heterogeneous nucleation effect; however, the two-dimensional （2D） GNSs might restrict the mobility of the PHBV chains in the process of crystal growing. Dynamic mechanical analysis studies showed that the storage modulus of the PHBV/GNSs nanocomposites was greatly improved.

关键词： PHBV Graphene nanosheets Nanocomposites Solution-casting films.