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Transcriptome-Wide Identification and Functional Analysis of PgSQE08-01 Gene in Ginsenoside Biosynthesis in Panax ginseng ***.

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Phyton-International Journal of Experimental Botany 2024年第2期93卷 313-327页

作者： Lei Zhu Lihe Hou yu Zhang Yang Jiang Yi Wang Meiping Zhang Mingzhu Zhao Kangyu Wang College of Life Science Jilin Agricultural UniversityChangchun130118China Jilin Engineering Research Center Ginseng Genetic Resources Development and Utilization Changchun130118China Jilin Academy of Vegetable and Flower Sciences Changchun130033China

Panax ginseng *** an important plant species used in traditional Chinese medicine,whose primary active ingredient is a *** biosynthesis is not only regulated by transcription factors but also controlled by a variety of structural ***,the molecular mechanism underlying ginsenoside biosynthesis has always been a topic in the discussion of ginseng secondary *** epoxidase(SQE)is a key enzyme in the mevalonic acid pathway,which affects the biosynthesis of secondary metabolites such as *** ginseng transcriptome,expression,and ginsenoside content databases,this study employed bioinformatic methods to systematically analyze the genes encoding SQE in *** first selected six PgSQE candidates that were closely involved in ginsenoside biosynthesis and then identified PgSQE08-01 to be highly associated with ginsenoside ***,we constructed the overexpression vector pCAMBIA3301-PgSQE08-01 and the RNAi vector pART27-PgSQE08-01 and transformed ginseng adventitious roots using Agrobacterium rhizogenes,to obtain positive hairy-root ***,quantitative reverse transcriptionpolymerase chain reaction and high-performance liquid chromatography were used to determine the expression of relevant genes and ginsenoside content,***,we focused on the function of PgSQE08-01 gene,which was noted to be involved in ginsenoside ***,these findings not only provided a molecular basis for the identification of important functional genes in ginseng but also enriched genetic resources for the biosynthesis of ginsenosides using synthetic biology.

关键词： Panax ginseng pgSQE08-01 gene squalene epoxidase ginsenoside ginseng hairy roots

不同精子来源及授精方式对剩余胚胎继续囊胚培养结局的影响

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生殖与避孕 2016年第10期36卷 845-851页

作者：郑炜炜谭玉梅祝晓丽陈瑞玲谭颖姜荣华刘善文吴铮宋革广东省计划生育专科医院生殖医学中心广州510060

目的:探讨不同精子来源及不同授精方式对胚胎继续发育能力的影响。方法:分析499例患者499个取卵周期剩余胚胎继续培养形成囊胚的情况,按精子来源不同分为供精IVF(D-IVF)组和夫精IVF(H-IVF)组,按授精方式不同分为IVF组和ICSI组,ICSI组按... 详细信息

目的:探讨不同精子来源及不同授精方式对胚胎继续发育能力的影响。方法:分析499例患者499个取卵周期剩余胚胎继续培养形成囊胚的情况,按精子来源不同分为供精IVF(D-IVF)组和夫精IVF(H-IVF)组,按授精方式不同分为IVF组和ICSI组,ICSI组按精子来源分为新鲜精液组、附睾精子和睾丸精子组,比较不同精子来源及授精方式获得剩余胚胎的囊胚形成率、胚胎利用率和无囊胚移植率。结果:① D-IVF组和H-IVF组受精率、卵裂率、优质胚胎率、第3日和第5日胚胎种植率、临床妊娠率和流产率均无统计学差异(P>0.05),组间剩余胚胎囊胚形成率、胚胎利用率和无囊胚移植率亦无统计学差异(P>0.05);②ICSI组与IVF组比较,其受精率较高(P0.05),但ICSI组与IVF组比较,其剩余胚胎囊胚形成率、胚胎利用率稍低,无囊胚移植率较IVF组稍高,差异有统计学意义(56.13%vs 65.32%,48.18%vs 55.39%,21.68%vs 13.20%,P0.05)。结论:D-IVF可获得H-IVF相似的结局,其剩余胚胎都有较高的发育潜能,ICSI获得的剩余胚胎发育潜能低于IVF组。附睾精子和睾丸精子ICSI后获得的胚胎比新鲜精液精子ICSI后胚胎发育潜能高。针对不同的授精方式可能需要制定相应的剩余胚胎囊胚培养标准。

关键词：剩余胚胎囊胚供精体外受精(IVF) 卵胞质内单精子注射(ICSI)

Multi-cohort comprehensive analysis unveiling the clinical value and therapeutic effect of GNAL in glioma

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Oncology Research 2024年第5期32卷 965-981页

作者： ZHEN LIU LIANGWANG YANG ZHENGXING XIE HUI yu TIANYI GU DAOMING SHI NING CAI SHENGHUA ZHUO Department of Neurosurgery Affiliated Hospital of Jiangsu UniversityZhenjiang212000China Department of Neurosurgery First Affiliated Hospital of Hainan Medical UniversityHaikou570100China Department of Cardiothoracic Surgery Affiliated Hospital of Jiangsu UniversityZhenjiang212000China Department of General Surgery Affiliated Hospital of Jiangsu UniversityZhenjiang212000China

Clinical data indicates that glioma patients have poor treatment outcomes and clinical *** role of olfactory signaling pathway-related genes(OSPRGs)in glioma has not been fully *** this study,we aimed to investigate the role and relationship between OSPRGs and *** and multivariate Cox regression analyses were performed to assess the relationship between OSPRGs and the overall survival of glioma based on public cohorts,and the target gene(G Protein Subunit Alpha L,GNAL)was *** association of GNAL expression with clinicopathological characteristics,gene mutation landscape,tumor immune microenvironment(TIME),deoxyribonucleic acid(DNA)methylation,and naris-occlusion controlled genes(NOCGs)was *** was used to evaluate GNAL level in *** analysis was conducted to evaluate the drug sensitivity,immunotherapy response,and functional enrichment of *** was an independent prognostic factor,and patients with low GNAL expression have a poor *** of GNAL was closely associated with clinicopathological characteristics,DNA methylation,and several immune-related *** infiltration analysis indicated that GNAL levels were negatively correlated with immune *** low-expression group showed efficacy with anti-PD-1 *** compounds with significantly different half-maximal inhibitory concentration(IC50)values between the GNAL high and low-expression groups were ***,its expression was associated with several immune cells,immune-related genes,and *** expression of GNAL is closely associated with clinicopathological characteristics,TIME,and the response to therapeutic interventions,highlighting its potential as a prognostic biomarker for glioma.

关键词： Glioma Olfaction GNAL Tumor immune microenvironment Immunotherapy/chemotherapy response

A Pricing-Based Cooperative Relay Selection Scheme for Reliable Communications

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China Communications 2024年第8期21卷 30-44页

作者： Xiao yulong Wu yu Amr Tolba Chen Ziqiang Li Tengfei School of Communications and Information Engineering Chongqing University of Posts and TelecommunicationsChongqing 400065China School of Cyber Security and Information Law Chongqing University of Posts and TelecommunicationsChongqing 400065China Department of Computer Science Community CollegeKing Saud UniversityRiyadh 11437Saudi Arabia

With the rapid development and application of energy harvesting technology,it has become a prominent research area due to its significant benefits in terms of green environmental protection,convenience,and high safety and ***,the uneven energy collection and consumption among IoT devices at varying distances may lead to resource imbalance within energy harvesting networks,thereby resulting in low energy transmission *** enhance the energy transmission efficiency of IoT devices in energy harvesting,this paper focuses on the utilization of collaborative communication,along with pricing-based incentive mechanisms and auction *** propose a dynamic relay selection scheme,including a ladder pricing mechanism based on energy level and a Kuhn-Munkre Algorithm based on an auction theory employing a negotiation mechanism,to encourage more IoT devices to participate in the collaboration *** results demonstrate that the proposed algorithm outperforms traditional algorithms in terms of improving the energy efficiency of the system.

关键词： cooperative communication edge net-work energy harvesting relay selection

Landscape of Sequence Variations in Homologous Copies of FAD2 and FAD3 in Rapeseed(Brassica napus L.)Germplasm with High/Low Linolenic Acid Trait

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Phyton-International Journal of Experimental Botany 2024年第3期93卷 627-640页

作者： Haoxue Wu Xiaohan Zhang Xiaoyu Chen Kang Li Aixia Xu Zhen Huang Jungang Dong Chengyu yu College of Agronomy Northwest A&F UniversityYangling712100China

Genetic manipulation(either restraint or enhancement)of the biosynthesis pathway ofα-linolenic acid(ALA)in seed oil is an important goal in Brassica napus *** is a tetraploid plant whose genome often har-bors four and six homologous copies,respectively,of the two fatty acid desaturases FAD2 and FAD3,which con-trol the last two steps of ALA biosynthesis during seed oil *** this study,we compared their promoters,coding sequences,and expression levels in three high-ALA inbred lines 2006L,R8Q10,and YH25005,a low-ALA line A28,a low-ALA/high-oleic-acid accession SW,and the wildtype *** expression levels of most FAD2 and FAD3 homologs in the three high-ALA accessions were higher than those in ZS11 and much higher than those in A28 and *** three high-ALA accessions shared similar sequences with the pro-moters and CDSs of BnFAD3.C4 and *** A28 and SW,substitution of three amino acid residues in BnFAD2.A5 and BnFAD2.C5,an absence of BnFAD2.C1 locus,and a 549 bp long deletion on the BnFAD3.A3 promoter were *** proﬁle of BnFAD2 mutation in the two low-ALA accessions A28 and SW is different from that reported in previous *** mutations in BnFAD3 in the high-ALA accessions are reported for theﬁrst *** identifying the sites of these mutations,we provide detailed information to aid the design of mole-cular markers for accelerated breeding schemes.

关键词： Brassica napus linolenic acid FAD2 FAD3 promoter coding sequences mutation

A nonenzymatic electrochemical sensor for the detection of hydrogen peroxide in vitro and in vivo fibrosis models

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Chinese Chemical Letters 2024年第3期35卷 394-398页

作者： Hongyao Liu Yan yu Taixiong Xue Cailing Gan yuting Xie Doudou Wang Peilin Li Zhiyong Qian Tinghong Ye Department of Gastroenterology and Hepatology Sichuan University-University of Oxford Huaxi Joint Centre for Gastrointestinal Cancer and Frontiers Science Center for Disease-Related Molecular NetworkState Key Laboratory of BiotherapyWest China HospitalSichuan UniversityChengdu 610041China Department of Biotherapy Cancer Center and State Key Laboratory of BiotherapyWest China HospitalSichuan UniversityChengdu 610041China

Fibrosis occurs due to the excessive deposition of extracellular matrix caused by cell *** various types of tissue injury,the dysregulation of the internal response can eventually lead to the destruction of organ structure and *** is increasing evidence that oxidative stress,which is characterized by excessive production of hydrogen peroxide(H_(2)O_(2)),is an important cause of ***,we synthesized a biosensitive and efficient electrochemical H_(2)O_(2)sensor based on PtNi nanoparticle-doped N-reduced graphene oxide(PtNi-N-rGO)to detect H_(2)O_(2)released from transforming growth factorβ1(TGFβ1)-induced *** addition,the sensor could easily detect changes in H_(2)O_(2)in the lung and bronchoalveolar lavage fluid(BALF)of mice with pulmonary ***,the sensor could also detect H_(2)O_(2)in activated hepatic stellate cells and the liver of carbon tetrachloride(CCl_(4))-induced liver ***,the alterations in H_(2)O_(2)detected by the sensor were consistent with nicotinamide adenine dinucleotide phosphate oxidase 4(NOX4)protein expression and the staining results of pathological *** together,these results highlight the use of H_(2)O_(2)sensors for the rapid detection of fibrosis and facilitate the rapid evaluation of antifibrotic drug candidates.

关键词： Fibrosis H_(2)O_(2) Electrochemical sensor NOX4 Nintedanib

Hierarchical Homochiral Assembly of Polyhedral Cage-Type Nanoclusters

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CCS Chemistry 2024年第10期6卷 2537-2548页

作者： Cheng-Long Deng Bao-Liang Han Ze-yu Liu Zhong-Hua Pan Jiao He yu-Long Li Zhi-Lin Yang Geng-Geng Luo Chen-Ho Tung Di Sun Lan-Sun Zheng Key Laboratory of Environmentally Friendly Function Materials Ministry of EducationCollege of Materials Science and EngineeringHuaqiao UniversityXiamen 361021 School of Chemistry and Chemical Engineering State Key Laboratory of Crystal MaterialsShandong UniversityJi’nan 250100 College of Physical Science and Technology Xiamen UniversityXiamen 361005 College of Chemistry and Environmental Engineering Sichuan University of Science and EngineeringZigong 643000 State Key Laboratory for Physical Chemistry of Solid Surfaces Department of ChemistryCollege of Chemistry and Chemical EngineeringXiamen UniversityXiamen 361005

Hierarchical chiral self-assembly is of profound significance for biomolecules for their biological ***-precise metal nanoclusters(NCs)offer better opportunities to construct hierarchical ***,achieving hierarchical homochiral assembly of nanocrystals protected by achiral ligands has proven to be a formidable task,with existing examples primarily limited to heterochiral *** we put forward a hierarchical assembly strategy towards precisely fabricating highly ordered homochiral ***,clusterin-nanocage type copper-hydride NCs act as synthons,of which entity is featured with a hexagonal close-packed Cu_(9)kernel embedded in a C_(3)-symmetric trigonal-prismatic metallacage,leading to a chiral core-shell primary structure with either P or M *** four homochiral NCs combined with guests through synergistic noncovalent interactions spontaneously organize into a supramolecular tetrahedral secondary structure within a unit ***,these nanoscopic supramolecular motifs pack into tertiary hierarchical superlattice of chiral cubic crystalline ***,the crystalline material shows excellent robustness,and fascinating blueexcitable near-infrared thermally activated delayed fluorescence *** work not only exhibits that nanocage-type NCs can be smart as proteins to translate chiral primary structure into tertiary chiral hierarchical complexity,but also provides new insights into structure-to-superstructure,enabling bottom-up creation of higher-level hierarchical homochiral superlattice structures.

关键词： polyhydrido copper nanoclusters chiral polyhedral metallacage hierarchical homochiral assembly thermally activated delayed fluorescence

First complete genomic sequence analysis of canine distemper virus in wild boar

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Virologica Sinica 2024年第4期39卷 702-704页

作者： Tong Wang Haiying Du Na Feng yuhang Liu yu Xu Heting Sun Peng Peng Siyuan Qin Xiaotian Zhang Yan Liu Mingyuan yu Hongrui Liang Biao He Guoqiang Zhu Changchun Tu Zhongzhong Tu Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Disease and Zoonoses Yangzhou UniversityYangzhou 225009China Changchun Veterinary Research Institute Chinese Academy of Agricultural SciencesChangchun 130122China Biological Disaster Control and Prevention Center National Forestry and Grassland AdministrationShenyang 110034China

Dear Editor,Canine distemper virus(CDV)is the causal agent of a highly contagious viral infectious disease that affects domestic and wild carnivores *** is an enveloped,non-segmented negative sense RNA virus that belongs to the Morbillivirus genus in Paramyxoviridae family,which contains viruses of epidemiological relevance to humans and *** on the variability of the hemagglutinin gene(H),CDV strains have been at least 21 major genetic lineages:America-1 to America-5,Canada-1 and-2,Asia-1 to Asia-6,Europe wild-life,Arctic,Africa-1/South Africa,Africa-2,South America-1 to South America-3 and Rockborn-like(Giacinti et al.,2022).Mutations in the binding sites of the H protein,which interact with viral entry receptors such as signaling lymphocytic activation molecule(SLAM)and nectin-4,are associated with the emergence of the disease in new host species(Beineke et al.,2015).

关键词： distemper Canada globally

肌肉注射鹿瓜多肽注射液所致不良反应原因分析及护理

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临床医学工程 2012年第3期19卷 454-455页

作者：谭宇王永红谭静文李家洁吴红英广州市正骨医院广东广州510045

目的观察并分析肌肉注射鹿瓜多肽注射液患者所产生不良反应及原因。方法观察我科半年内所有肌肉注射此药物的患者,随机分为餐后组(1029人次)和空腹组(515人次),分析比较两组患者肌肉注射鹿瓜多肽后产生不良反应的比例。结果不论餐后或空... 详细信息

目的观察并分析肌肉注射鹿瓜多肽注射液患者所产生不良反应及原因。方法观察我科半年内所有肌肉注射此药物的患者,随机分为餐后组(1029人次)和空腹组(515人次),分析比较两组患者肌肉注射鹿瓜多肽后产生不良反应的比例。结果不论餐后或空腹,大多数患者都有局部不良反应发生,且空腹组患者的不良反应发生率明显高于餐后组;空腹注射此药物的患者有78%以上出现全身性不良反应。结论空腹肌肉注射鹿瓜多肽后的不良反应明显,建议餐后注射,以保证临床用药的安全;出现不良反应时,应及时、有效地进行对症处理。

关键词：鹿瓜多肽肌肉注射不良反应护理