检索结果-南通市图书馆

Generation of mitochondrial replacement monkeys by female pronucleus transfer

在线全文

学校读者我要写书评

暂无评论

Zoological Research 2024年第2期45卷 292-298页

作者： Chun-Yang Li Xing-Chen Liu yu-Zhuo Li Yan Wang Yan-Hong nie yu-Ting Xu Xiao-Tong Zhang Yong Lu Qiang Sun Institute of Neuroscience Center for Excellence in Brain Science and Intelligence TechnologyState Key Laboratory of NeuroscienceChinese Academy of SciencesShanghai 200031China Key Laboratory of Genetic Evolution and Animal Models Chinese Academy of SciencesKunming 650201China University of Chinese Academy of Sciences Beijing 100049China

Mutations in mitochondrial DNA(mtDNA)are maternally inherited and have the potential to cause severe disorders.Mitochondrial replacement therapies,including spindle,polar body,and pronuclear transfers,are promising strategies for preventing the hereditary transmission of mtDNA diseases.While pronuclear transfer has been used to generate mitochondrial replacement mouse models and human embryos,its application in non-human primates has not been previously reported.In this study,we successfully generated four healthy cynomolgus monkeys(Macaca fascicularis)via female pronuclear transfer.These individuals all survived for more than two years and exhibited minimal mtDNA carryover(3.8%–6.7%),as well as relatively stable mtDNA heteroplasmy dynamics during development.The successful establishment of this nonhuman primate model highlights the considerable potential of pronuclear transfer in reducing the risk of inherited mtDNA diseases and provides a valuable preclinical research model for advancing mitochondrial replacement therapies in humans.

关键词： Non-human primates Mitochondrial replacement Female pronuclear transfer

Long non-coding RNA BANCR promotes pancreatic cancer lymphangiogenesis and lymphatic metastasis by regulating the HIF-1α/VEGF-C/VEGFR-3 pathway via miR-143-5p

同方期刊数据库博看期刊评论

在线全文

学校读者我要写书评

暂无评论

Genes & Diseases 2024年第4期11卷 57-60页

作者： Shaolong Hao yu Ji Weixuan Pan Hao Sun Fang nie Jonathan Ray Warren yuchuan Ding Wei Han Department of General Surgery Beijing Luhe HospitalCapital Medical UniversityTongzhouBeijing 101149China Department of Central Laboratory Beijing Luhe HospitalCapital Medical UniversityTongzhouBeijing 101149China Department of Neurosurgery Wayne State University School of MedicineJohn D.Dingell VA Medical CenterDetroitMI 48201USA

Pancreatic cancer is one of the most aggressive malignant tumors of the digestive system.Lymph node metastasis(LNM),as the main way of invasion and metastasis,can occur in the early stage of pancreatic cancer and be considered the most important factor affecting the clinical treatment and prognosis of patients with pancreatic cancer.

关键词： metastasis cancer invasion

Fe/S oxidation-coupled arsenic speciation transformation mediated by AMD enrichment culture under different pH conditions

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Environmental Sciences 2024年第3期137卷 681-700页

作者： yu-hang Zhou Wei-xi Huang Zhen-yuan nie Hong-chang Liu yue Liu Can Wang Jin-lan Xia Wen-sheng Shu Key Lab of Biometallurgy of Ministry of Education of China School of Minerals Processing and BioengineeringCentral South UniversityChangsha 410083China School of Life Sciences South China Normal UniversityGuangzhou 510631China

Arsenic(As)speciation transformation in acid mine drainage(AMD)is comprehensively affected by biological and abiotic factors,such as microbially mediated Fe/S redox reactions and changes in environmental conditions(pH and oxidation-reduction potential).However,their combined impacts on arsenic speciation transformation remain poorly studied.Therefore,we explored arsenic transformation and immobilization during pyrite dissolution mediated by AMD enrichment culture under different acidic pH conditions.The results for incubation and mineralogical transformation of solid residues show that in the presence of AMD enrichment culture,pH 2.0,2.5,and 3.0 are more conducive to the formation of jarosites and ferric arsenate,which could immobilize high quantities of dissolved arsenic by adsorption and coprecipitation.The pH conditions significantly affect the initial adsorption of microbial cells to the minerals and the evolution of microbial community structure,further infuencing the biodissolution of pyrite and the release and oxidation process of Fe/S.The results of Fe/S/As speciation transformation of the solid residues show that the transformation of Fe,S,and As in solution is mainly regulated by pH and potential values,which imposed significantly different effects on the formation of secondary minerals and thus arsenic oxidation and immobilization.The above results indicated that arsenic transformation is closely related to the Fe/S oxidation associated with pyrite bio-oxidation,and this correlation is critically regulated by the pH conditions of the system.

关键词： Acid mine drainage(AMD) pH conditions Fe/S/As speciation transformation Microbial community structure

Synthesis of Chiral Hydantoins and Thiazolidinediones via Iridium-Catalyzed Asymmetric Hydrogenation

在线全文

学校读者我要写书评

暂无评论

Chinese Journal of Chemistry 2022年第7期40卷 819-824页

作者： yu nie Jing Li Qianjia yuan Wanbin Zhang Shanghai Key Laboratory of Molecular Engineering of Chiral Drugs Frontiers Science Center for Transformative MoleculesSchool of Chemistry and Chemical EngineeringShanghai Jiao Tong University800 Dongchuan RoadShanghai 200240China College of Chemistry Zhengzhou University75 Daxue RoadZhengzhouHenan 450052China

Herein,we report an iridium-catalyzed asymmetric hydrogenation of hydantoin and thiazolidinedione derived exocyclic alkenes using our developed BiphPHOX as a ligand.The transformation shows good functional group tolerance,and gives the hydrogenated prod-ucts with excellent yields(up to 99%)and enantioselectivities(up to 98%ee).A gram-scale reaction was also carried out,and pro-vided the hydrogenated product in excellent yield with no erosion in enantioselectivity.Finally,the transformation of the hydrogen-ated product provided an efficient approach for the synthesis of the intermediate of a HIV protease inhibitor.

关键词： Hydantoins Thiazolidinediones Phosphine-oxazoline ligand Iridium Asymmetric catalysis

CRISPR-CasRx knock-in mice for RNA degradation

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Science China(Life Sciences) 2022年第11期65卷 2248-2256页

作者： Jiacheng Li Dekun Zhu Shengshou Hu yu nie State Key Laboratory of Cardiovascular Disease Fuwai Hospital and Cardiovascular InstituteNational Center for Cardiovascular DiseasesChinese Academy of Medical Sciences&Peking Union Medical CollegeBeijing 100037China National Health Commission Key Laboratory of Cardiovascular Regenerative Medicine Fuwai Central-China HospitalCentral-China Branch of National Center for Cardiovascular DiseasesZhengzhou 450046China

The RNA editing tool CRISPR-CasRx has provided a platform for a range of transcriptome analysis tools and therapeutic approaches with its broad efficacy and high specificity.To enable the application of CasRx in vivo,we established a Credependent CasRx knock-in mouse.Using these mice,we specifically knocked down the expression of Meis1 and Hoxb13 in cardiomyocytes,which induced cardiac regeneration after myocardial infarction.We also knocked down the lnc RNA Mhrt in cardiomyocytes with the CasRx knock-in mice,causing hypertrophic cardiomyopathy.In summary,we generated a Credependent CasRx knock-in mouse that can efficiently knock down coding gene and lnc RNA expression in specific somatic cells.This in vivo CRISPR-CasRx system is promising for gene function research and disease modeling.

关键词： CRISPR-CasRx CasRx mice in vivo gene knockdown heart regeneration

Anchoring Ru clusters to highly defective N-doped carbon nanotubes via a thermal-shock strategy for stable industrial hydrogen evolution

在线全文

学校读者我要写书评

暂无评论

Nano Research 2024年第6期17卷 5261-5269页

作者： Zhiming Li Xinyu Li Haiqing Ma Chenliang Ye Hongan yu Long nie Meng Zheng Jin Wang College of Materials Science and Engineering Shenzhen UniversityShenzhen 518060China School of Chemistry and Materials Science Anhui Normal UniversityWuhu 241002China Department of Power Engineering North China Electric Power UniversityBaoding 071003China

Non-Pt or low-Pt catalysts capable for stable generation of hydrogen via water electrolysis at an industrial level of current density are highly demanded.Construction of strong metal-support connection is beneficial to improve the performance stability of electrocatalysts.Here we employed highly defective N-doped carbon nanotubes(d-N-CNT)as the support to achieve uniform and firm anchoring of Ru clusters(~1.9 nm)via a thermal-shock strategy.The as-prepared Ru/d-N-CNT catalyst shows excellent catalytic activity for hydrogen evolution reaction(HER)in alkaline media and requires an overpotential(ƞ)of 12 mV at 10 mA·cm^(−2)and 116 mV at 200 mA·cm^(−2)with a Ru loading of 0.025 mg·cm^(−2).Impressively,Ru/d-N-CNT presents robust stability for HER at both low current density(stable for at least 1000 h at 10 mA·cm^(−2))and the industrial level of current density(stable for at least 100 h at 1000 mA·cm^(−2)),remarkably outperforming commercial Pt/C and Ru/C.The highly defective nature of the N-CNT support endowed the as-prepared Ru/d-N-CNT catalyst with strong metal-support adhesion that efficiently suppressed agglomeration as well as obscission of Ru clusters.Meanwhile,the rich defects increased the surface energy of the N-CNT support and resulted in improved hydrophilicity as evidenced by the liquid contact angle measurement and the bubble evolution process,which also played an important role in stabilizing the HER performance especially at large current density.

关键词： thermal-shock method hydrogen evolution reaction large-current-density Ru cluster

Rice planting acceleration of the activation and loss of soil iron in the red soil region of southern China

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Soil Ecology Letters 2024年第2期6卷 179-187页

作者： San’an nie Jie Fan Ningxiang Ouyang Hao Sheng Yangzhu Zhang Xiong Yan Zhan yu College of Resources Hunan Agricultural UniversityChangsha410125China School of Economic Geography Hunan University of Finance and EconomicsChangsha410205China Zunyi Normal University Zunyi563006China

Rice planting decreased total iron but increased active iron.Iron activation varied greatly among different paddy soils but not in woodland soils.Paddy soil iron was mainly affected by pH,SOC and particle composition.... 详细信息

关键词： paddy soil iron forms parent material rice planting Fe activation

Effects of aspirin on colon cancer usingquantitative proteomic analysis

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Cancer Pathogenesis and Therapy 2024年第2期2卷 121-131页

作者： Yan Zhang Haitao Sun yu Ji Fang nie Rong Wang Wei Han Central Laboratory Beijing Luhe HospitalCapital Medical UniversityBejing 101149China Department of General Surgery Beijing Luhe HospitalCapital Medical UniversityBejing 101149China Central Laboratory Xuanwu HospitalCapital Medical UniversityBeijing 100053China

Background:Colon cancer is one of the most prevalent digestive cancers worldwide.Results of epidemiological,experimental,and clinical studies suggest that aspirin inhibits the development of colon cancer.This study aimed to systematically elucidate the molecular mechanisms by which aspirin prevents colon carcinogenesis.Methods:We determined the global protein expression profiles of colorectal cancer and aspirin-treated cells using quantitative proteomic analysis.We analyzed the proteomic results using bioinformatics(including differential pro-teins,protein annotation,Kyoto Encyclopedia of Genes and Genomes[KEGG]pathways,and protein-protein inter-action[PPI]network).The viability of the colon cancer cell line and HT29 cells treated with aspirin was determined using the cell counting kit-8 assay.The differentially expressed proteins,such as p53 and cyclin-dependent kinase 1(CDK1),were quantified using real-time polymerase chain reaction(PCR)and Western blotting.We measured cell cycle distribution and apoptosis in HT29 cells exposed to aspirin using fluorescence-activated cell sorting(FACS).Results:We found that 552 proteins were significantly dysregulated,of which 208 and 334 were upregulated and downregulated,respectively,in colon cancer cells exposed to 10 mmol/L of aspirin(95%confidence interval[CI]:-1.269 to-0.106,P<0.05).Further gene enrichment analysis revealed that cell cycle-related proteins,such as p53 and CDK1,were significantly differentially expressed.Proteomic analysis showed that after 24 h of aspirin exposure,the level of p53 increased by 2.52-fold and CDK1 was downregulated to half that of the controls in HT29 cells(95%CI:-0.619 to-0.364,P<0.05).Real-time PCR and Western blotting results showed that p53 was upregulated(95%CI:-3.088 to-1.912,P<0.001)and CDK1 was significantly downregulated after aspirin exposure in colon cancer cells(95%CI:0.576 to 1.045,P<0.05).We observed that aspirin promoted G1/S cell cycle arrest in HT29 cells.We confirmed that aspirin induces apoptosis in human HT29 colon cance

关键词： Colon cancer Proteomics Aspirin Cell cycle Cell apoptosis

Effects of Milling Methods on Rice Flour Properties and Rice Product Quality:A Review

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Rice science 2024年第1期31卷 33-46页

作者： TIAN yu SUN Jing LI Jiaxin WANG Aixia nie Mengzi GONG Xue WANG Lili LIU Liya WANG Fengzhong TONG Litao Institute of Food Science and Technology/Western Research Institute Chinese Academy of Agricultural Sciences/Key Laboratory of Agro-Products ProcessingMinistry of Agriculture and Rural AffairsBeijing 100193China

High-quality rice flour is the foundation for the production of various rice-based products.Milling is an essential step in obtaining rice flour,during which significant changes occur in the physicochemical and quality characteristics of the flour.Although rice flour obtained through mainstream wet milling methods exhibits superior quality,low production efficiency and wastewater discharge limit the development of the industry.Dry milling,on the other hand,conserves water resources,but adversely affects flour performance due to excessive heat generation.As an emerging powder-making technique,semi-dry milling offers a promising solution by enhancing flour quality and reducing environmental impact.This is achieved by minimizing soaking time through hot air treatment while reducing mechanical energy consumption to reach saturated water absorption levels.However,continuous production remains a challenge.This comprehensive review summarizes the effects of various milling technologies on rice flour properties and product qualities.It also discusses key control indicators and technical considerations for rice flour processing equipment and processes.

关键词： flour property milling equipment milling method rice flour rice product quality semi-dry milling