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Immune responses to the expressed products of the CSP antigen gene of Plasmondium falciparum south in China isolate FCC1/HN in Hela cell

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南通大学学报(医学版) 2003年第1期 115-115页

作者： yu xb Liu YW Luo SH

Objective:To construct a eukaryotic expression system with pcDNA3-PfCSP/Hela for the Circumsporozoite protein(CSP) gene of Plasmodium falciparum(***),to observe the immune responses in BALB/c mice induced by the expressed proteins. Methods:The recombinant plasmid pcDNA3-PfCSP was transformed into the Hela cell *** expressed protein was isolated and analyzed by using SIDS-PAGE and used for immunization of BALB/c mice by subcutaneous, intravenous, and intraperitoneal ***-linked immunosorbent assay(ELISA),Dot-ELISA,Western blot,T lymphocyte proliferation test,natural killer cell(NKC) activity assay,and CD4(+) and CD8(+) T cell detection were used for observation of humoral and cellular immune responses. Results:Immune sera strongly reacted with the expressed protein,antibody titer was up to 1:6400 as detected by *** blot analysis revealed a specific band at 38.3 *** the spleen cells of normal and immunized BALB/c mice were specifically stimulated with expressed protein,the optical densities were 0.12+/-0.03 and 0.34+/-0.04,*** latter were significantly higher than the former(P<0.01).We used the MTT colormetric assay to measure NKC activity of mice *** results showed that the NKC activity of immunized BALB/c mice was remarkably higher than that of the controls(P<0.05).CD4(+) and CD8(+) T cells were detected by using monoclonal antibody immunofluorscence *** results showed that the percentage of CD4(+) and CD8(+) T cells of immunized group were significantly higher than that of control group(P<0.05).Conclusions:The humoral and cell-mediated immune responses and elevated NKC activity to products made with a eukaryotic expression sytem could be specifically detected in BALB/c *** findings indicated that the expressed protein could enhance the immune function in mice.

关键词： plasmodium falciparum circumsporozoite protein Held cell expressed product immune response

环孢素A和他克莫司对移植肾P-糖蛋白表达的影响:3年随访移植肾活检

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中国组织工程研究与临床康复 2007年第8期11卷 1435-1438,I0002页

作者：张波刘敏张炜顾民俞香宝孙彬邢昌赢南京医科大学第一附属医院肾脏病科江苏省南京市210029 南京医科大学第一附属医院泌尿外科肾移植中心江苏省南京市210029

目的：观察环孢素A和他克莫司对人类移植肾组织P-糖蛋白表达影响的差异,分析两种药物肾毒性差异的分子作用途径。方法：实验于2005-01/12在南京医科大学临床肾脏病学实验室完成。选择在南京医科大学第一附属医院泌尿外科肾移植中心接受... 详细信息

目的：观察环孢素A和他克莫司对人类移植肾组织P-糖蛋白表达影响的差异,分析两种药物肾毒性差异的分子作用途径。方法：实验于2005-01/12在南京医科大学临床肾脏病学实验室完成。选择在南京医科大学第一附属医院泌尿外科肾移植中心接受肾移植的患者60例,肾移植后时间均在3年以上,根据移植后免疫抑制方案不同将其分为2组,环孢素A组32例患者采用强的松（5mg/d）＋骁悉（1.5g/d）＋环孢素A（初始剂量为5mg/（kg·d））免疫抑制治疗;他克莫司组28例患者采用强的松＋骁悉＋他克莫司（初始剂量为0.1mg/（kg·d））免疫抑制治疗,强的松、骁悉剂量同环孢素A组。①记录两组患者的一般临床资料。②移植肾活检,比较肾组织慢性移植肾排斥的班夫评分（Ⅰ级：间质浸润同时发现中度或重度动脉炎病灶;Ⅱ级：轻度或重度内膜炎或严重的动脉内膜炎并涉及〉25%的腔体;Ⅲ级：跨壁动脉内膜炎和/或动脉纤维样变和内侧平滑肌细胞坏死伴随淋巴细胞炎症）及平均慢性化病变积分（由小管萎缩、动脉病变、间质纤维化3项评分得出的均值,其中每项0-3分,依次表示〈25%,26%-50%,51%-75%和〉75%的观察视野）。③免疫组织染色检测移植肾P-糖蛋白的表达并借助图像分析软件比较其在近端肾小管染色强度（半定量为0-4级,0为阴性,4为最强）的差异。结果：60例患者全部进入结果分析。①环孢素A组血肌酐、尿蛋白排泄量及血脂水平显著高于他克莫司组（P〈0.05）,肌酐清除率显著低于他克莫司组（P〈0.05）。②肾毒性指标小管萎缩、动脉透明样变、间质纤维化的发病人数环孢素A组显著高于他克莫司组（P〈0.05）,环孢素A组慢性化病变积分也显著高于他克莫司组（P〈0.05）。③P-糖蛋白主要分布于肾小管顶膜、基底膜和细胞浆内。环孢素A组的染色强度显著低于他克莫司组（3.1±0.7,3.8±0.4,P〈0.01）。结论：应用环孢素A进行肾移植术后免疫抑制的患者发生神经钙调蛋白抑制剂肾毒性的比率和程度均明显高于他克莫司组,其中P-糖蛋白的表达水平低下是其可能的分子作用途径。

关键词：肾移植环孢菌素他罗利姆 P-糖蛋白

Magnetotelluric sounding in western Tibet

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Chinese Science Bulletin 1997年第8期42卷 678-681页

作者： Ma, xb Kong, XR yu, S Institute of Geophysics Chinese Academy of Sciences Beijing China

THE magnetotelluric sounding(MT)profile from Gyirong to Sangehu in western Tibet indi-cates that the surveyed region is divided into four tectonic units by Yarlung Zangbo SutureZone(S.Z.),Bangongco-Nujiang *** Lugu **... 详细信息

关键词： magnetotelluric sounding conductivity structure of lithosphere high conductivity layer Tibet Gandise unit Qiangtang unit.

Redox Reaction of Disulfide/Polyaniline in Aqueous Solution

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Chinese Chemical Letters 1998年第6期9卷 523-526页

作者： yu, L Wang, XH Li, J Jing, xb Wang, FS Chinese Acad Sci Changchun Inst Appl Chem Polymer Chem Lab Changchun 130022 Peoples R China

The cleavage and formation of the di sulfide bond of 2,5-dimercapto-1,3,4-thiadiazole (DMcT) were examined in an aqueous solution of pH value from 0 to 14 with and without polyaniline (PAn), The redox reaction of DMcT was accelerated by PAn in acidic condition. The cell using this anodic material was set-up and characterized in aqueous electrolyte.

关键词： polyaniline disulfide redox reaction

Three-dimensional cytoplasmic calcium propagation with boundaries

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Communications in Theoretical Physics 2021年第1期73卷 87-98页

作者： Han-yu Jiang Jun He Department of Physics and Institute of Theoretical Physics Nanjing Normal UniversityNanjing210097China Sino-U.S.Center for Grazingland Ecosystem Sustainability/Pratacultural Engineering Laboratory of Gansu Province/Key Laboratory of Grassland Ecosystem Ministry of Education/College of Pratacultural ScienceGansu Agricultural UniversityLanzhou730070China

Ca^(2+)plays an important role in cell signal *** intracellular propagation is the most basic process of Ca^(2+)signaling,such as calcium wave and double messenger *** this work,with both numerical simulation and mean field ansatz,the three-dimensional probability distribution of Ca^(2+),which is read out by phosphorylation,is studied in two scenarios with *** coverage of distribution of Ca^(2+)is found at an order of magnitude ofμm,which is consistent with experimental observed calcium spike and *** results suggest that the double messenger system may occur in the ER-PM junction to acquire great *** buffer effect of kinase is also discussed by calculating the average position of phosphorylations and free Ca^(2+).The results are helpful to understand the mechanism of Ca^(2+)signaling.

关键词： Ca^(2+)signaling diffusion Gillespie algorithm mean field ansatz

Isolation and characterization of the Secale NPR1 （Sc NPR1） from a Chinese rye variety JinzhouHeimai

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Isolation and characterization of the Secale NPR1 （Sc NPR1...

Nonlinear signal transduction network with multistate

江苏省遗传学会第八届会员代表大会暨学术研讨会

作者： X Zhang,HX Ma,L Xu,xb Sun,GH yu Institute of Biotechnology,Jiangsu Academy of Agricultural Sciences,Nanjing,210014,P.R.China.

＜正＞The development of transgenic wheat plants highly resistant to a range of pathogens using traditional signal gene expression strategies has been largely *** of systemic acquired resistance（SAR） through the over expression of a controlling gene such as NPR1（non-expressor of PR genes） offers an attractive alternative for augmenting the wheat plants innate defense *** find new gene resource for wheat pathogen-resistance genetic engineering,Secale cereale *** was used as a starting material to clone full-length cDNA of NPR1 gene by homologous cloning and smarter-RACE *** the ScNPR1 molecule,there are four"W- boxes"in the upstream of ATG that are necessary for both induction of NPR1 transcription and NPR1-mediated activation of plant defense *** gene contains the BTB and ankyrin repeat domain that is known to be the molecular basis for NPR1 function in *** predicted amino acid sequence oi ScNPR1 had highest sequence similarity to TaNPRI and OsNPR1 with 72%and 65%of identities but lower homology（37%） to the AtNPR1 proteins.

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Chinese Physics B 2021年第11期30卷 666-675页

作者： Han-yu Jiang Jun He School of Physics and Technology Nanjing Normal UniversityNanjing 210097China

Signal transduction is an important and basic mechanism to cell life *** stochastic state transition of receptor induces the release of signaling molecular,which triggers the state transition of other *** constructs a nonlinear sigaling network,and leads to robust switchlike properties which are critical to biological *** architectures and state transitions of receptor affect the performance of this biological *** this work,we perform a study of nonlinear signaling on biological polymorphic network by analyzing network dynamics of the Ca^(2+)-induced Ca^(2+)release(CICR)mechanism,where fast and slow processes are involved and the receptor has four conformational *** types of networks,Erdos–R´enyi(ER)network,Watts–Strogatz(WS)network,and BaraB´asi–Albert(BA)network,are considered with different *** dynamics of the biological networks exhibit different patterns at different time *** short time scale,the second open state is essential to reproduce the quasi-bistable regime,which emerges at a critical strength of connection for all three states involved in the fast processes and disappears at another critical *** pattern at short time scale is not sensitive to the network *** long time scale,only monostable regime is observed,and difference of network architectures affects the results more *** finding identifies features of nonlinear signaling networks with multistate that may underlie their biological function.

关键词： signal transduction biological network with multistate CICR nonlinear signaling

A New Expression of the Hermitian Solutions to a System of Matrix Equations with Applications

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Algebra Colloquium 2016年第1期23卷 167-180页

作者： Guangjing Song Shaowen yu Ming Chen School of Mathematics and Information Sciences Weifang University Weifang Shandong 261061 China Department of Mathematics East China University of Science and Technology Shanghai 200237 China College of Mathematics Physics and Information Engineering Jiaxing University Jiaxing Zhejiang 314000 China

In this paper, a new necessary and sufficient condition for the existence of a Hermitian solution as well as a new expression of the general Hermitian solution to the system of matrix equations A1X = C1 and A3xb3 = C3 are derived. The max-min ranks and inertias of these Hermitian solutions with some interesting applications are shown. In particular, the max-min ranks and inertias of the Hermitian part of the general solution to this system are presented.

关键词： matrix equations linear matrix expression generalized inverse inertias

Synthesis of substitutional hexaboride of lanthanum and cerium La_(1-x)Ce_(x)B_(6) via aluminothermic reduction

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Journal of Rare Earths 2023年第11期41卷 1780-1788,I0005页

作者： yu Wang Xiao-Hui Yang Jian Cui Guo-Hua Zhang State Key Laboratory of Advanced Metallurgy University of Science and Technology BeijingBeijing100083China Beijing Key Laboratory of Green Recovery and Extraction of Rare and Precious Metals University of Science and Technology BeijingBeijing 100083China

Rare-earth hexaborides(REB_(6))are vital raw materials for cathode materials and high temperature structural ceramics that are widely applied as high-frequency electron tubes and ceramics adaptive for extreme environment,*** this work,single phase substitutional solid solution REB_(6)(LaB_(6),La_(0.75)Ce_(0.25)B_(6).La_(0.5)Ce_(0.5)B_(6),La_(0.25)Ce_(0.75)B_(6) and CeB_(6))powders were prepared with the raw materials of La_(2)O_(3),CeO_(2),B_(4)C and Al powders,after calcining at 1773 K for 4 h and the following alkaline *** substitutional solid solution products have homogeneous distributions of La and Ce in *** microscopic morphology analysis,it is discovered that the formation of solid solution is beneficial for reducing the particle size of product,relative to LaB_(6) and CeB_(6).Moreover,Al flux plays an important role in decarbonizing reaction,and C contents of all products are below 0.4 wt%.

关键词： Hexaboride Substitutional solid solution Aluminothermic reduction Rare earths