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Analyses of Genetic Diversities in Chinese Fresh-water Fishes

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生物多样性 1996年第B10期4卷 1-5页

作者： yu qixing HOU RONGJIADepartment of Biology,Wuhan University,Wuhan,430072

A wealth of literature has been published in the studies of Chinese fresh-water fish chromosomes within recent *** this paper,we review the inter-and intraspecific genetic diversities found in Chinese fresh-water fishes,and explore the characteristics and mechanisms that underline these diversities and variations. We also propose the hypothesis how the genetic diversities had evolved in the fresh-water fishes.

关键词：遗传多样性淡水鱼生物多样性变种

PCR Amplification of Sox Genes in Misgurnus anguillicaudatus and Paramisgurnus dabryanus(Cypriniform;Cobitidae)

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Wuhan University Journal of Natural Sciences 1997年第2期2卷 128-130页

作者： Chang Zhongjie Zhou Rongjia yu qixing College of Life Sciences Wuhan University Wuhan 430072China

Using the primers which specially amplify the conservative motif of Human SRYgene,we stud ied the PCR amplification of Sox genes in genomic DNA of two species of mud loach Misgurus anguillicau-datus and Paramisgurnus *** bands with the length of 200,550,940 and 1000 bp respectively,were presented in the PCR products of Misgumus *** bands with the length of 200,550 and 900 bp were presented in that of Paramisgumus *** blotting results indicated that the 200 and 550 bp bands are specially *** is no difference between male and female individuals as well as between these two species.

关键词： Sox gene Misgurnus anguillicaudatus Paramisgumus dabryanus

The Karyotypes, C-banding Patterns and AgNORs of Epinephelus malabaricus

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High Technology Letters 2004年第2期10卷 1-4页

作者：邹记兴 Hu Chaoqun Xiang Wenzhou yu qixing Zhou Fei South China Sea Institute of Oceanology Chinese Academy of Sciences Guangzhou 510301 P.R. China College of Life Sciences Wuhan University Wuhan 430072 P.R. China

The chromosome specimens of Epinephelus malabaricus (Bloch & Schneider, 1801) are obtained from metaphase of kindney cell by vivi-injection of PHA and culture of colchicines, hypatoic-air drying technique, and then by studying their Giemsa stain, C-bands and AgNORs. The results are as follows: (1)E. malabaricus has a diploid chromosome number of 48 and its karyotype formula is 48t, NF=48, sex chromosome is not found. (2) There is a pair of chromosomes with secondary constriction near the centromere of chromosome t24. (3) 1～4 nucleoli appear in the nucleus of interphase, 55% nuclei has 1 nucleolus and only 2% for 4 nucleoli. (4) AgNORs appear in the chromosome t24 of 50% metaphase, sometimes in the chromosome t5, but not in other chromosomes. (5) The AgNORs polymorphisms are individually specific, 1～4 pairs of the number, and the frequency of 4 AgNORs are lowest. (6) The secondary constrictions and positive C-bands are coincident, close to the centromere of the chromosome, and mass constrictive heterochromatins appear in that region. (7) All the centromeres of chromosomes are darkly stained C-bands, and the whole arm of chromosome t24 and its centromere are same positive C-bands. (8) The evolutive regulation of the karyotype and the developing mechanism of AgNORs and C-bands are discussed.

关键词：染色体组型 C-带黑带石斑鱼鱼类 AgNORs

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蚕豆姐妹染色单体区分染色的孚尔根显示法

武汉大学学报(自然科学版) 1984年第3期 109-112页

作者：余其兴

本文报道蚕豆姐妹染色单体分染（SCD）的一种改良技术,即BrdU—Feulgen法。种子萌发后,剪取主根根尖,用清水培养,当大多数侧根长0.5—1.5厘米时,放入BrdU(5×10-4M)溶液中培养两个细胞周期,随后用秋水仙素预处理,甲醇—冰醋酸固定,再在3... 详细信息

本文报道蚕豆姐妹染色单体分染（SCD）的一种改良技术,即BrdU—Feulgen法。种子萌发后,剪取主根根尖,用清水培养,当大多数侧根长0.5—1.5厘米时,放入BrdU(5×10-4M)溶液中培养两个细胞周期,随后用秋水仙素预处理,甲醇—冰醋酸固定,再在37℃的3.5NHC1中延长水解90—110分钟,以Schiff试剂染色,压片。此法具有简便、快速和分染效果较好的特点。其分染结果是与FPG法以及BrdU—Giemsa法相反的B—浓染型。本文还探讨了蚕豆细胞短周期问题,以及蚕豆SCE作为诱变因素检测手段的实际应用价值。

关键词：染色单体蚕豆佛豆分染姐妹

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鳑鲏亚科四种鱼的C-带研究

武汉大学学报（自然科学版） 1991年第2期37卷 107-110,T002页

作者：韦萍余其兴武汉大学生物系

本文报道了高体鰟鮍,斑条剌鰟鮍、兴凯刺鰟鮍、大鳍刺鰟鮍的C-带带型研究结果。发现鰟鮍亚科2n=44及2n=42的鱼类结构异染色质的量及其分布都非常相似,均与2n=48的鱼类有很大差别。由此对结构异染色质与核型演化的关系作了充分探讨,认为... 详细信息

本文报道了高体鰟鮍,斑条剌鰟鮍、兴凯刺鰟鮍、大鳍刺鰟鮍的C-带带型研究结果。发现鰟鮍亚科2n=44及2n=42的鱼类结构异染色质的量及其分布都非常相似,均与2n=48的鱼类有很大差别。由此对结构异染色质与核型演化的关系作了充分探讨,认为鰟鮍亚科鱼类的核型演化机制主要是着丝粒融合。此外,首次报道了斑条刺鰟鮍的核型。

关键词：鱼类 Pang Pi亚科 C带核型演化

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PCR扩增泥鳅和大鳞副泥鳅SRY盒基因

动物学杂志 1998年第1期33卷 12-15页

作者：常重杰周荣家余其兴武汉大学生命科学学院

以特异扩增人ＳＲＹ基因保守区的一对引物，研究了泥鳅和大鳞副泥鳅基因组中ＳＲＹ盒基因的扩增。结果表明，该引物可以在泥鳅中扩增出四条带，其长度分别为２００，５５０，９４０和１０００ｂｐ。在大鳞副泥鳅中扩增出三条带，大小为... 详细信息

以特异扩增人ＳＲＹ基因保守区的一对引物，研究了泥鳅和大鳞副泥鳅基因组中ＳＲＹ盒基因的扩增。结果表明，该引物可以在泥鳅中扩增出四条带，其长度分别为２００，５５０，９４０和１０００ｂｐ。在大鳞副泥鳅中扩增出三条带，大小为２００，５５０和９００ｂｐ。经Ｓｏｕｔｈｅｒｎ杂交显示出二者的阳性带为２００和５５０ｂｐ。阳性带在雌雄个体间和两个物种间无差异。

关键词： SRY盒基因泥鳅大鳞副泥鳅 PCR

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即食鱼胶产品的杀菌工艺

水产学报 2024年第2期48卷 180-188页

作者：袁毅姜启兴高沛杨方余达威许艳顺夏文水江南大学食品学院江南大学江苏省食品安全与质量控制协同创新中心

为了改进鱼胶的热杀菌工艺，减少鱼胶杀菌后品质的劣化。实验以美国红鱼鱼胶为对象，确定了在F (杀菌强度)为4.5 min时不同杀菌温度下的杀菌工艺，通过物性分析、低场核磁分析和红外色谱分析等方法，研究了不同杀菌温度对鱼胶质构、色... 详细信息

为了改进鱼胶的热杀菌工艺，减少鱼胶杀菌后品质的劣化。实验以美国红鱼鱼胶为对象，确定了在F (杀菌强度)为4.5 min时不同杀菌温度下的杀菌工艺，通过物性分析、低场核磁分析和红外色谱分析等方法，研究了不同杀菌温度对鱼胶质构、色泽、胶原蛋白含量、水分分布以及二级结构的影响。结果显示，为达到F=4.5 min，在110、115、121和1和125°C下，鱼胶产品分别需要杀菌56.44、15.85、3.46和0.92 min，杀菌后的鱼胶产品均能达到商业无菌状态。在110～125°C，杀菌后的鱼胶能保持一定的蛋白结构。随着杀菌温度的升高，鱼胶的亮度L*值和b*值分别由59.78和17.80逐渐下降至49.65和11.25,a*值无显著变化；鱼胶的硬度和剪切力显著上升、黏性下降，经125°C杀菌后分别为37.02 g和34.24 g·s，黏性为-2.82 g；样品的胶原蛋白含量上升、析出减少，110和125°C杀菌后鱼胶中胶原蛋白的含量分别为7.62%和14.92%，溶液中胶原蛋白浓度分别为3.33和0.15mg/mL。110°C杀菌后鱼胶内水分的自由度最高，鱼胶内自由水的比例随杀菌温度的升高而下降。研究表明，即食鱼胶在较高的温度下杀菌后亮度降低，但维持了更好的质构以及更高的胶原蛋白含量，保持了较好的品质。本研究对于促进鱼胶的加工利用，提高杀菌即食水产品的品质具有重要的实践意义。

关键词：眼斑拟石首鱼鱼胶即食杀菌温度胶原蛋白品质

胶子球候选者θ/f_2(1720)的D波分量和可能的实验检验

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高能物理与核物理 1989年第11期13卷 996-1002页

作者：郁宏沈齐兴王旭中国科学院高能物理所

本文讨论了J/φ辐射衰变中胶子球候选者θ/f_2(1720)的产生及其衰变.我们发现,为了解释过程J/φ→r+θ的螺旋度振幅之比x和y,除了θ粒子的S波分量之外,必须考虑两个D波分量(ι=2,s=0,2)的贡献.我们还讨论了D’波分量(ι=2,s=2)是否存在... 详细信息

本文讨论了J/φ辐射衰变中胶子球候选者θ/f_2(1720)的产生及其衰变.我们发现,为了解释过程J/φ→r+θ的螺旋度振幅之比x和y,除了θ粒子的S波分量之外,必须考虑两个D波分量(ι=2,s=0,2)的贡献.我们还讨论了D’波分量(ι=2,s=2)是否存在的可能的实验检验.

关键词：胶子球 D波分量辐射衰变

Effects of methamidophos and glyphosate on copper sorption-desorption behavior in soils

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Science China(Life Sciences) 2005年第Z1期48卷 67-75页

作者： yu Ying, ZHOU qixing & HE Zhenli Key Laboratory of Terrestrial Ecological Process, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China College of Natural Resources and Environmental Sciences, Zhejiang University, Hangzhou 310029, China Key Laboratory of Terrestrial Ecological Process Institute of Applied Ecology Chinese Academy of Sciences Shenyang 110016 China College of Natural Resources and Environmental Sciences Zhejiang University Hangzhou 310029 China

A batch-equilibration technique was employed to study the impact of two organophosphorus pesticides methamidophos (MDP) and glyphosate (GPS) on copper (Cu2+) sorptiondesorption for phaeozem and burozem collected from Northeastern China. The addition of the two pesticides decreased Cu2+ sorption, increased Cu2+ desorption and prolonged the equilibrium time of Cu2+ sorption-desorption. But GPS appeared to exert a stronger influence on Cu2+ sorption-desorption due to its stronger complexion with Cu2+. When MDP was added, Cu2+ sorption-desorption was linearly correlated with MDP treatment concentrations. But in the presence of GPS, Cu2+ sorption first underwent a rapid decrease period, and then slowly tended towards a steady period. The reverse pattern could be found for Cu2+ desorption in the presence of *** pesticides and with the existence of MDP, Cu2+ sorption-desorption kinetics was well conformed to two-constant equation and Elovich equation. But that was not the case for Cu2+ desorption kinetics in the presence of GPS although its sorption could be also described by these two equations.

关键词： Cu, sorption, desorption, methamidophos, glyphosate, phaeozem, burozem.