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Rice pollen-specific OsRALF17 and OsRALF19 are essential for pollen tube growth

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Journal of Integrative Plant Biology 2023年第9期65卷 2218-2236页

作者： Eui‐Jung kim Ji‐Hyun kim Woo‐Jong Hong Eun Young kim Myung‐Hee kim Su Kyoung Lee Cheol Woo Min Sun Tae kim Soon Ki Park Ki‐Hong Jung yu‐jin kim Graduate School of Green Bio‐Science&Crop Biotech Institute Kyung Hee UniversityYongin 17104Republic of Korea Department of Life Science and Environmental Biochemistry Life and Industry Convergence Research InstitutePusan National UniversityMiryang 50463Republic of Korea Department of Smart Farm Science Kyung Hee UniversityYongin 17104Republic of Korea School of Applied Biosciences Kyungpook National UniversityDaegu 41566Republic of Korea Genomics Division Department of Agricultural Bio‐ResourcesNational Institute of Agricultural SciencesRural Development AdministrationJeonju 54874Republic of Korea Department of Plant Bioscience Pusan National UniversityMiryang 50463Republic of Korea Research Center for Plant Plasticity Seoul National UniversitySeoul 08826Republic of Korea

Pollen tube growth is essential for successful double fertilization, which is critical for grain yield in crop plants. Rapid alkalinization factors(RALFs) function as ligands for signal transduction during fertilization. However, functional studies on RALF in monocot plants are ***, we functionally characterized two pollen-specific RALFs in rice(Oryza sativa) using multiple clustered regularly interspaced palindromic repeats(CRISPR)/CRISPR-associated protein 9-induced loss-of-function mutants,peptide treatment, expression analyses, and tag reporter lines. Among the 41 RALF members in rice, OsRALF17 was specifically expressed at the highest level in pollen and pollen *** applied OsRALF17 or OsRALF19peptide inhibited pollen tube germination and elongation at high concentrations but enhanced tube elongation at low concentrations, indicating growth regulation. Double mutants of OsRALF17 and OsRALF19(ralf17/19) exhibited almost full male sterility with defects in pollen hydration, germination, and tube elongation,which was partially recovered by exogenous treatment with OsRALF17 peptide. This study revealed that two partially functionally redundant OsRALF17 and OsRALF19 bind to Oryza sativa male-gene transfer defective 2(OsMTD2) and transmit reactive oxygen species signals for pollen tube germination and integrity maintenance in rice. Transcriptomic analysis confirmed their common downstream genes, in osmtd2 and ralf17/19. This study provides new insights into the role of RALF, expanding our knowledge of the biological role of RALF in regulating rice fertilization.

关键词： CRISPR-Cas9 CrRLK1L monocot Oryza sativa pollen tube growth rapid alkalinization factor(RALF)

A pilot study of single-use endoscopy in screening acute gastrointestinal bleeding

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World Journal of Gastroenterology 2013年第1期19卷 103-107页

作者： Jae Hee Cho Hee Man kim Sangheun Lee yu jin kim Ki Jun Han Hyeon Geun Cho Si Young Song Division of Gastroenterology Department of Internal MedicineMyongji HospitalKwandong University College of MedicineGoyang 412-270South Korea Department of Internal Medicine Graduate SchoolYonsei University College of MedicineSeoul 120-752South Korea Division of Gastroenterology Department of Internal MedicineBrain Korea 21 Project for Medical ScienceSeverance Biomedical Science InstituteYonsei University College of MedicineSeoul 120-752South Korea

AIM:To investigate the feasibility of a single-use endoscopy as an alternative procedure to nasogastric lavage in patients with acute gastrointestinal(GI) ***:Patients who presented with hematemesis,melena or hematochezia were enrolled in this *** scan and conventional esophagogastroduodenoscopy(EGD) were subsequently *** bleeding was defined as blood in the stomach,and inactive bleeding was defined as coffee ground clots and clear fluid in the *** findings were recorded and ***:Between January and March,2011,13 patients that presented with hematemesis(n = 4),melena(n = 6),or bleeding from a previous nasogastric feeding tube(n = 3),were enrolled in this *** 12 patients with upper GI bleeding,the EG scan device revealed that 7 patients had active bleeding and 5 patients had inactive bleeding,whereas conventional EGD revealed that 8 patients had active bleeding and 4 patients had inactive *** sensitivity and specificity of the EG scan device was 87.5% and 100% for active bleeding,with conventional EGD serving as a *** complication were reported during the EG scan ***:The EG scan is a feasible device for screening acute upper GI *** may replace nasogastric lavage for the evaluation of acute upper GI bleeding.

关键词： Gastrointestinal hemorrhage Bleeding Endoscopy Nasogastric tube Lavage

Seroprevalence of anti-HAV among patients with chronic viral liver disease

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World Journal of Gastroenterology 2011年第2期17卷 236-241页

作者： Hyun Chin Cho Seung Woon Paik yu jin kim Moon Seok Choi Joon Hyeok Lee Kwang Cheol Koh Byung Chul Yoo Hee Jung Son Seon Woo kim Division of Gastroenterology Samsung Medical CenterSungkyunkwan University School of Medicine Division of Gastroenterology Center for Health PromotionSamsung Medical CenterSungkyunkwan University School of Medicine Biostatics Team Samsung Medical CenterSungkyunkwan University School of Medicine

AIM:To investigate the current seroprevalence of hepatitis A virus(HAV) antibodies in patients with chronic viral liver disease in *** also tried to identify the factors affecting the prevalence of HAV antibodies. METHODS:We performed an analysis of the clinical records of 986 patients(mean age:49±9 years,714 males/272 females) with chronic hepatitis B virus(HBV) or hepatitis C virus(HCV) infection who had undergone HAV antibody testing between January 2008 and December ***:The overall prevalence of IgG anti-HAV was 86.61%(854/986) in patients with chronic liver disease and was 88.13%(869/986) in age-and gendermatched patients from the Center for Health *** anti-HAV prevalence was 80.04%(405/506) in patients with chronic hepatitis B,86.96%(20/23) in patients with chronic hepatitis C,93.78%(422/450) in patients with HBV related liver cirrhosis,and 100%(7/7) in patients with HCV related liver *** anti-HAV prevalence according to the decade of age was as follows:20s(6.67%) ,30s(50.86%) ,40s(92.29%) ,50s(97.77%) ,and 60s(100%) .The antiHAV prevalence was significantly higher in patients older than 40 years compared with that in patients younger than 40 years of *** analysis showed that age≥40 years,female gender and metropolitan cities as the place of residence were independent risk factors for IgG anti-HAV seropositivity. CONCLUSION:Most Korean patients with chronic liver disease and who are above 40 years of age have already been exposed to hepatitis A virus.

关键词： Chronic hepatitis B Chronic hepatitis C Hepatitis A virus Korea Seroprevalence

Hepatobiliary scintigraphy for detecting biliary strictures after living donor liver transplantation

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World Journal of Gastroenterology 2011年第21期17卷 2626-2631页

作者： yu jin kim Kyu Taek Lee Young Cheol Jo Kwang Hyuck Lee Jong Kyun Lee Jae-Won Joh Choon Hyuck David Kwon Department of Medicine Samsung Medical CenterSungkyunkwan University School of Medicine Department of Surgery Samsung Medical CenterSungkyunkwan University School of Medicine

AIM:To investigate the diagnostic accuracy of hepatobiliary scintigraphy(HBS) in detecting biliary strictures in living donor liver transplantation(LDLT) ***:We retrospectively reviewed 104 adult LDLT recipients of the right hepatic lobe with duct-toduct anastomosis,who underwent HBS and *** HBS results were categorized as normal,parenchymal dysfunction,biliary obstruction,or bile leakage without *** presence of biliary strictures was determined by percutaneous cholangiography or endoscopic retrograde cholangiopancreatography(ERCP).RESULTS:In 89 patients with biliary strictures,HBS showed biliary obstruction in 50 and no obstruction in 39,for a sensitivity of 56.2%.Of 15 patients with no biliary strictures,HBS showed no obstruction in 11,for a specificity of 73.3%.The positive predictive value(PPV) was 92.6%(50/54) and the negative predictive value(NPV) was 22%(11/50).We also analyzed the diagnostic accuracy of the change in bile duct *** sensitivity,NPV,specificity,and PPV were 65.2%,27.9%,80% and 95%,***:The absence of biliary obstruction on HBS is not ***,when post-LDLT biliary strictures are suspected,early ERCP may be considered.

关键词： Living donor liver transplantation Tc99m mebrofenin Radionucleotide imaging Hepatobiliary scintigraphy Biliary stricture

Tenofovir rescue therapy for chronic hepatitis B patients after multiple treatment failures

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World Journal of Gastroenterology 2012年第47期18卷 6996-7002页

作者： yu jin kim Dong Hyun Sinn Geum-Youn Gwak Moon Seok Choi Kwang Cheol Koh Seung Woon Paik Byung Chul Yoo Joon Hyeok Lee Department of Medicine Samsung Medical CenterSungkyunkwan University School of MedicineSeoul 135-710South Korea

AIM:To evaluate the efficacy and safety of tenofovir disoproxil fumarate(TDF) for chronic hepatitis B(CHB) patients after multiple ***:A total of 29 CHB patients who had a suboptimal response or developed resistance to two or more previous nucleoside/nucleotide analogue(NA) treatments were *** subjects were treated with TDF alone(n = 13) or in combination with lamivudine(LAM,n = 12) or entecavir(ETV,n = 4) for ≥ 6 *** virologic response(CVR) was defined as an achievement of serum hepatitis B virus(HBV) DNA level ≤ 60 IU/mL by real-time polymerase chain reaction method during *** assessment was based on serum creatinine and phosphorus *** patients had histories of LAM and adefovir dipivoxil(ADV) treatment and 18 patients were exposed to LAM,ADV,and ***-seven patients(93.1%) were hepatitis B e antigen(HBeAg) positive and the mean value of the baseline serum HBV DNA level was 5.5 log IU/mL ± 1.7 log IU/*** median treatment duration was 16 mo(range 7 to 29 mo).RESULTS:All the patients had been treated with LAM and developed genotypic and phenotypic resistance to *** to ADV was present in 7 patients and 10 subjects had a resistance to *** patient had a resistance to both ADV and *** cumulative probabilities of CVR at 12 and 24 mo of TDF containing treatment regimen calculated by the Kaplan Meier method were 86.2% and 96.6%,*** one patient failed to achieve CVR,serum HBV DNA level decreased by 3.9 log IU/mL from the baseline and the last serum HBV DNA level during treatment was 85 IU/mL,achieving near *** patients in this study showed viral breakthrough or primary non-response during the follow-up *** cumulative probability of HBeAg clearance in the 27 HBeAg positive patients was 7.4%,12%,and 27% at 6,12,and 18 mo of treatment,*** efficacy of TDF containing regimen was not statistically different according to the presence of specific HBV mutatio

关键词： Tenofovir Chronic hepatitis B Treatment failure

Prevalence and prognosis of acute pancreatitis in critically ill patients with COVID-19

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Hepatobiliary & Pancreatic Diseases International 2023年第4期22卷 399-402页

作者： Dongju Kang SuHo Park Changkyo Oh yujin kim jinBae kim SangHoon Park Myung Seok Lee JaeKeun Park Department of Internal Medicine Kangnam Sacred Heart HospitalHallym University College of MedicineSeoul 07441Korea

Background:Coronavirus disease 2019(COVID-19)is a global pandemic *** addition to the well-known respiratory and fever symptoms,gastrointestinal symptoms have also been *** study aimed to evaluate the prevalence and prognosis of patients with COVID-19 infection complicated with acute pancreatitis in intensive care unit(ICU).Methods:This was a retrospective observational cohort study,and patients aged 18 years or older,ad-mitted into the ICU in a single tertiary center from January 1,2020,to April 30,2022 were *** were identified by electronic medical records and reviewed *** primary outcome was the prevalence of acute pancreatitis among ICU patients with *** secondary outcomes were the length of hospital stay,need for mechanical ventilation(MV),need for continuous renal replacement therapy(CRRT),and in-hospital ***:A total of 4133 patients,admitted into the ICU,were *** these patients,389 were infected by COVID-19,and 86 were diagnosed with acute ***-19 positive patients were more likely to present with acute pancreatitis than COVID-19 negative patients(odds ratio=5.42,95%confidence interval:2.35-6.58,P<0.01).However,the length of hospital stay,need for MV,need for CRRT,and in-hospital mortality were not significantly different between acute pancreatitis patients with and without COVID-19 ***:Severe COVID-19 infections may cause acute pancreas damage in critically ill ***-ever,the prognosis may not differ between acute pancreatitis patients with and without COVID-19 infec-tion.

关键词： Acute pancreatitis COVID-19 Coronavirus infections Intensive care unit

Anti-inflammatory activity of ginsenosides in LPS-stimulated RAW 264.7 cells

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Science Bulletin 2015年第8期60卷 773-784,M0003页

作者： Sungeun Ahn Muhammad Hanif Siddiqi Hae-Yong Noh yu-jin kim Yeon-Ju kim Chi-Gyu jin Deok-Chun Yang Ginseng Genetic Bank Graduate School of BiotechnologyCollege of Life Science Kyung Hee University

Ginsenosides, the main active constituents of Panax ginseng Meyer （P. ginseng）, have potential therapeutic effects. All tested ginsenosides except gin- senoside F1 have previously been reported in inflammation studies using the RAW 264.7 macrophage cell line. We ex- amined the anti-inflammatory effects of single sugar moiety ginsenosides such as compound K （CK）, Rh2, Rhl, and F1 that were isolated from P. ginseng through in silico docking studies. We investigated their biological activity predictions, including absorption, distribution, metabolism, excretion, and toxicity and PASS properties, on the suppression of NF- κB, followed by in vitro validation in lipopolysaccharide （LPS）-stimulated RAW 264.7 macrophage cells. The molecular docking results of our study showed that all treated ginsenosides are non-toxic and may be drug-like molecules. The molecular binding interactions of these ginsenosides with the active residues of NF-κB noticeably support their anti-inflammatory activity. CK and Rhl sig- nificantly reduced the production of nitric oxide, cyclooxy- genase-2 （COX-2）, and pro-inflammatory cytokines such as prostaglandin E2 and tumor necrosis factor alpha （TNF-α） in a dose-dependent manner. Real-time PCR and Western blot analyses further confirmed that protopanaxadiols （PPDs） and protopanaxatriols （PPTs） inhibitory effects may have been due to the down-regulation of TNF-α, inducible nitric oxide synthase, COX2, nuclear factor kappa B （NF-κB）, and I kappa B kinase. The expression of co-stimulatory molecules such as ROS was also inhibited by CK and Rhl in a dose- dependent manner. Furthermore, activation of NF-κB in LPS-stimulated RAW 264.7 macrophages was significantly suppressed by CK and Rhl. Taken together, these results provide evidence that PPD- and PPT-type ginsenosides in- cluding CK and Rhl may exhibit strong anti-inflammatory effects by inhibiting pro-inflammatory mediators through down-regulation of NF-κB.

关键词： Ginsenosides Protopanaxadiol Protopanaxatriol Inflammation NF-κB/IKK RAW 264.7

The Expression of Matrix Metalloproteinases and Tissue Inhibitors of Metalloproteinases in Idiopathic Interstitisal Pneumonia

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Open Journal of Respiratory Diseases 2014年第3期4卷 101-109页

作者： Ji Young Shin yu jin kim Sun Young Kyung Seung Yeon Ha Sung Hwan Jeong Department of Pathology Gachon University Gil Medical Center Incheon Republic of Korea Division of Pulmonology Department of Internal Medicine Gachon University Gil Medical Center Incheon Republic of Korea Division of Pulmonology Department of Internal Medicine Gachon University Gil Medical CenterIncheon Republic of Korea

Background: Idiopathic interstitial pneumonia is characterized by fibroblast proliferation and extracellular matrix (ECM) accumulation. Matrix metalloproteases (MMPs) and tissue inhibitors of metalloproteases (TIMPs) have been shown to regulate remodeling of the ECM, which indicates that they are important factors in the process of lung fibrosis. Therefore, we evaluated the expression of MMPs and TIMPs in tissues obtained from patients with idiopathic interstitial pneumonia and control tissues. Methods: Thirty-seven patients who were diagnosed with IIP (22: IPF, 13: NSIP, 2: COP) and 5 controls were enrolled in this study. The MMP-2 and -9 activity in lung tissue obtained from these patients was analyzed using gelatin zymography and the levels of TIMP-1 and -2 were measured by western blotting. We also evaluated the expression of MMP-2 and -9, as well as that of TIMP-1 and -2 in lung tissue using immunohistochemistry. Results: The levels of MMP-2 and MMP-9 were significantly increased in patients with IPF compared to those with NSIP and COP. The activities of TIMP-1 and -2 were also higher in patients with IPF than NSIP/COP patients and control subjects. There were no significant differences observed in the activities of MMPs and TIMPs obtained from patients with NSIP/COP and control subjects. The immunohistochemical analysis showed that TIMP-2 and MMP-2 were strongly stained at the fibroblasts of the fibroblastic foci in patients with IPF. Conclusions: These results suggest that over-expression of gelatinases and TIMPs in patients with IPF are important factors in the irreversible fibrosis that is associated with lung parenchyma.

关键词： Idiopathic Interstitial Pneumonia Idiopathic Pulmonary Fibrosis Matrix Metalloproteinases Tissue Inhibitor of Matalloproteinases Nonspecific Interstitial Pneumonia Cryptogenic Organizing Pneumonia

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使用汞分析器检测矿渣及水泥中的汞

生命科学仪器 2011年第4期9卷 24-26页

作者： Sang-Ho NAM yu-Na kim jin-Su NA Keun-Hyeok YANG Department of Chemistry College of Natural ScienceMokpo National University Department of Architecture Engineering Kyonggi University

以矿渣和水泥作为研究对象,采用汞分析器对其中的汞进行检测。水泥是一种建筑过程中广泛使用的高凝结材料。由于汞具有较大的毒性,因此必须严格检测建筑材料中汞的含量以降低其对人体的损害。作为水泥的替代物,矿渣具有许多的优点,例如... 详细信息

以矿渣和水泥作为研究对象,采用汞分析器对其中的汞进行检测。水泥是一种建筑过程中广泛使用的高凝结材料。由于汞具有较大的毒性,因此必须严格检测建筑材料中汞的含量以降低其对人体的损害。作为水泥的替代物,矿渣具有许多的优点,例如价格低廉、毒性较小等。

关键词：分析器水泥矿渣检测汞建筑材料替代物毒性