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紫花苜蓿耐盐性相关NAC转录因子的挖掘及表达分析

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草地学报 2024年第4期32卷 1055-1067页

作者：荣梦茹余如刚韦英铭王国良杜雪玲杨改梅高佳佳淮北师范大学生命科学学院安徽淮北235000 山东省农业科学院休闲农业研究所山东济南250010

NAC(NAM,ATAF1/2,CUC2)是植物特有的一类转录因子家族,可调控植物响应盐胁迫。本研究基于盐胁迫下紫花苜蓿(Medicago sativa L.)耐盐品种GIB和盐敏感品种LS根和叶的转录组数据,通过生物信息学方法筛选出17个差异表达的MsNAC转录因子家... 详细信息

NAC(NAM,ATAF1/2,CUC2)是植物特有的一类转录因子家族,可调控植物响应盐胁迫。本研究基于盐胁迫下紫花苜蓿(Medicago sativa L.)耐盐品种GIB和盐敏感品种LS根和叶的转录组数据,通过生物信息学方法筛选出17个差异表达的MsNAC转录因子家族成员,并对其序列特征、系统进化、顺式作用元件及盐胁迫下的表达模式等进行分析。结果显示,17个MsNACs均具有NAC典型的NAM结构域,且蛋白结构相似。系统进化分析将17个MsNACs分成8个亚族,各亚组成员具有高度相似的保守基序。顺式作用元件分析发现,有15个MsNACs具有ABA响应顺式元件ABRE。转录组数据表达模式分析与RT-qPCR验证结果显示MsNAC1,MsNAC2,MsNAC35,MsJUB1和MsNAC40基因在盐处理下GIB叶中上调表达,在LS叶中下调或者未发生变化,推测这些基因参与调控紫花苜蓿的耐盐反应。本研究为进一步研究紫花苜蓿NAC转录因子响应盐胁迫功能提供参考依据。

关键词：紫花苜蓿 NAC 盐胁迫生物信息学分析表达模式

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Comparative analysis of the microbiome of sympatric wintering Bean Geese,Domestic Ducks,humans,and soil at Shengjin Lake of China reveals potential public risk to human health

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Avian Research 2024年第2期15卷 279-286页

作者： gang Liu Na Xu Chongyang yu College of Life Sciences Anhui Medical UniversityHefei 230032China

The gut microbiota of migratory waterbirds is affected by various complex factors,including cross-species transmission,which increases the risk of pathogen spreading among sympatric animals and poses a potential public health risk to *** this study,we investigated the microbial communities of wintering Bean Geese(Anser fabalis),Domestic Ducks(*** domesticus),humans,and soil using high-throughput sequencing of the 16S rRNA gene region in Shengjin Lake,*** total,6,046,677 clean reads were obtained,representing 41,119 operational taxonomic units(OTUs)across the four *** dominant microbial phyla were the Proteobacteria,Firmicutes,Bacteroidota,and *** Sorensen similarity index and alpha and beta diversity results showed that the gut microbial communities of Bean Geese and Domestic Ducks were more similar to those of the other *** analysis revealed that Faecalibacterium prausnitzii,Pseudomonas fragi,and Bradyrhizobium elkanii were hubs of the three major *** common microbiomes were iden-tified in Bean Geese,Domestic Ducks,humans,and soil in Shengjin Lake.A total of 96 potential pathogens were identified among the four groups,with 20 specific potentially pathogenic microbiomes found in the gut of Bean *** of these pathogens are responsible for significant financial losses in the poultry industry and pose risks to human *** pneumoniae,Morganella morganii,Escherichia coli,and Ralstonia insidiosa are potential core pathogens found in the four groups at Shengjin Lake that can cause diseases in humans and an-imals and facilitate cross-species transmission through various ***,humans are at risk of con-tracting these pathogens from migratory birds because of their frequent contact with domestic ***,further studies are required to explore the potential pathogenic species and transmission pathways among sympatric wintering Bean Geese,Domestic Ducks,humans,and soil.

关键词： Microbiome Migratory waterbird Pathogens Poultry Sympatric species

通过精神分裂症差异基因建立针对结肠癌的预后分析及预测模型

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标记免疫分析与临床 2024年第1期31卷 167-175页

作者：沈静蔡辉俞刚潘巨龙陆根兴南京医科大学附属江苏盛泽医院江苏苏州215200

目的本研究旨在探索精神分裂症与结肠癌之间差异基因的相关性,以及这些差异基因对结肠癌发生发展的影响,并建立结肠癌预后生存的风险评估模型。方法从基因表达综合数据库(GEO)中选取精神分裂症(SCZ)数据集,并从TCGA数据库获得结肠癌(C... 详细信息

目的本研究旨在探索精神分裂症与结肠癌之间差异基因的相关性,以及这些差异基因对结肠癌发生发展的影响,并建立结肠癌预后生存的风险评估模型。方法从基因表达综合数据库(GEO)中选取精神分裂症(SCZ)数据集,并从TCGA数据库获得结肠癌(COAD)相关基因。对SCZ数据集进行Limma分析,鉴定差异表达基因(DEG)。通过机器学习鉴定最小绝对收缩和选择运算符(Lasso-Cox)回归用于识别与COAD生存预后相关的候选基因,并最终得到4个风险相关基因用于建立COAD的风险模型。绘制ROC曲线和Kaplan-Meier(KM)分析曲线用于评估模型的诊断效果和预后生存能力。然后建构预后模型并验证其预测性能。最后我们对这4个基因进行GSVA富集分析和免疫浸润分析以探讨其与COAD预后相关的可能机制。结果通过筛选,我们找到了4个与COAD预后相关的候选基因(ATP6V1B1、C1orf61、CCKBR、CRHR1),并构建了预测模型(C-index为:0.750)(AUC 1年0.84,AUC 3年0.80,AUC 5年0.80)(KM中P=2.3×10-10)。这些基因显示出较高的诊断和预测价值。此外,这4个基因与免疫细胞浸润有较强的关系,可能是导致COAD预后差异的原因。结论本研究成功建构了一个包含4个候选基因的预后模型,具有较高的诊断和预测价值。这些基因可能与免疫细胞浸润有关,为揭示COAD的预后差异提供了新的视角。

关键词：精神分裂结肠癌机器学习预后模型

Progress on the glassy-crystal laminates:From design,microstructure to deformation and future solutions

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Journal of Materials Science & Technology 2024年第5期172卷 113-144页

作者： yu Chen Ronggao Cui Jun Shen gang Wang College of Mechatronics and Control Engineering Shenzhen UniversityShenzhen 518060China College of Physics and Optoelectronic Engineering Shenzhen UniversityShenzhen 518060China Department of Motor Design Business Weichai New Energy Technology Co.Ltd.Weifang 261061China Guangdong Key Laboratory of Electromagnetic Control and Intelligent Robots Shenzhen UniversityShenzhen 518060China Laboratory for Microstructures Institute of MaterialsShanghai UniversityShanghai 200444China

The development of new design strategies to create innovative structural materials,refine existing ones,and achieves compatible combinations of strength and plasticity remains a worldwide *** alloys,such as shape memory alloys(SMAs),bulk metallic glasses(BMGs),high entropy alloys(HEAs),and heterogeneous pure metals such as Cu,have excellent mechanical responses,but they still fall short of meeting all the requirements of structural materials due to specific flaws,such as lack of tensile de-formation for BMGs and low yielding strength for *** address these shortcomings,proposals such as integrating glassy matrices and crystallized alloys,such as HEAs/SMAs,have been ***,these solutions have unresolved issues,such as the challenging control of B2 phase formation in BMG ***,glass-crystal(A/C)laminated alloys with alternating layers have been reported to exhibit improved mechanical properties and activated work-hardening behaviors,but they still face press-ing issues such as bonding interfaces and unknown deformation *** review focuses on design routes such as the selection of alloy components and processing techniques,exploration of micro-structural evolution and deformation modes with an increase in strain,and future solutions to address pressing and unsolved *** prominent advantages include diversified deformation mechanisms,such as deformation twinning,martensitic phase transformation,and precipitation hardening,as well as tuned interactive reactions of shear bands(SBs)near the A/C ***,this review provides a promising pathway to design and develop structural materials in the materials field community.

关键词： Glassy-crystal laminates Bulk metallic glasses High entropy alloys Shape memory alloys Microstructure Deformation mechanisms Future solutions

BIM技术在地下溶洞嵌岩桩基极限承载力评定中的应用

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粘接 2024年第7期51卷 165-168页

作者：虞刚方四发郭鹏陈永达武汉市市政建设集团有限公司湖北武汉430056 武汉磊尔科技发展有限公司湖北武汉430070

为了提高地下溶洞嵌岩桩基的安全性,提出了BIM技术在地下溶洞嵌岩桩基极限承载力评定中的应用。计算地下溶洞嵌岩桩基在岩层的平均应力,获取的承载机理,明确地下溶洞嵌岩的地质特点,计算地下溶洞嵌岩桩基容许承载力,利用BIM技术,完成地... 详细信息

为了提高地下溶洞嵌岩桩基的安全性,提出了BIM技术在地下溶洞嵌岩桩基极限承载力评定中的应用。计算地下溶洞嵌岩桩基在岩层的平均应力,获取的承载机理,明确地下溶洞嵌岩的地质特点,计算地下溶洞嵌岩桩基容许承载力,利用BIM技术,完成地下溶洞嵌岩桩基的BIM建模。根据地下溶洞嵌岩桩基应力分量,计算地下溶洞嵌岩桩基在含溶洞地层中的平均约束应力,通过设定极限承载力阈值,评定地下溶洞嵌岩桩基极限承载力。实验结果表明,所提方法对地下溶洞嵌岩桩基极限承载力的评定结果与工程实际相符,可以缩小评定误差。

关键词： BIM技术嵌岩桩基承载机理极限承载力平均约束应力

Visible-light-driven thio-carboxylation of alkynes with CO_(2):facile synthesis of thiochromones

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Science China Chemistry 2023年第5期66卷 1457-1466页

作者： Meng Miao Lei Zhu Hong Zhao Lei Song Si-Shun Yan Li-Li Liao Jian-Heng Ye yu Lan Da-gang yu Key Laboratory of Green Chemistry&Technology of Ministry of Education College of ChemistrySichuan UniversityChengdu 610064China College of Pharmacy Third Military Medical UniversityChongqing 400038China School of Chemistry and Chemical Engineering Chongqing Key Laboratory of Theoretical and Computational ChemistryChongqing UniversityChongqing 400030China College of Chemistry and Institute of Green Catalysis Zhengzhou UniversityZhengzhou 450001China

Difunctionalizing carboxylation of alkynes with CO_(2)is a sustainable and important strategy to generate valuable acrylate derivatives from both readily available starting *** protocols,however,always suffer from the use of excess metallic reagents and transition metal ***,we report the first thio-carboxylation of alkynes with thiophenols and CO_(2),which is a visible-light-driven and transition metal-free *** contrast to previous carboxylations of alkynes via two-electron activation of CO_(2),mechanistic and computational investigations suggest that the single-electron activation of CO_(2)is involved in the thio-carboxylation,rendering uniqueβ-*** following cyclizing acylation affords important thiochromones ***,the one-pot method features mild reaction conditions(room temperature,1 atmosphere of CO_(2)),high chemo-and regio-selectivity,easy scalability and facile derivatization of products to bioactive compounds.

关键词： carbon dioxide visible light carboxylation thiochromones

Effect of Maximal Oxygen Pulse on Patients with Chronic Obstructive Pulmonary Disease

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Biomedical and Environmental Sciences 2022年第9期35卷 830-841页

作者： LI yun Xiao WANG Jun WU Bo LIN Fang TAN Chun Ting yu gang gang NIE Shan ZHAO Ran Ran XU Bo Department of Respiratory Medicine Capital Medical University Affiliated Beijing Friendship HospitalBeijing 100050China

Objective This study evaluated the effect of maximal oxygen pulse(O_(2)P_(max))on patients with chronic obstructive pulmonary disease(COPD)and confirmed the predictive effect on acute exacerbations of COPD(AECOPD).Methods This retrospective study included 91 participants who underwent cardiopulmonary exercise testing(CPET),lung function testing,a dyspnea scale assessment,and a 3-year *** participants were divided into two groups according to the O_(2)P_(max)*** capacity,ventilatory conditions,gas exchange efficiency,and dyspnea symptoms were compared,and the correlations between O_(2)P_(max)and these indices were *** ability of O_(2)P_(max)to predict AECOPD was *** Exercise capacity,ventilatory conditions,and gas exchange efficiency were lower,and dyspnea symptom scores were higher in the impaired O_(2)P_(max)group(P<0.05).O_(2)P_(max)was positively correlated with forced vital capacity(FVC)%,forced expiratory volume in 1 sec(FEV_(1))%,FEV_(1)/FVC%,anaerobic threshold(AT),work rate(WR)%,aximal oxygen uptake(VO_(2))%,VO_(2)/kg_(max),VO_(2)/kg_(max)%,WR_(AT),WR_(max),VO_(2AT),VO_(2max),and V,and was negatively correlated with EqCO_(2AT),and EqCO_(2max)(P<0.05).Most importantly,O_(2)P_(max)could be used to predict AECOPD,and the best cut-off value was 89.5%(area under the curve,0.739;95%CI,0.609–0.869).Conclusion O_(2)P_(max)reflected exercise capacity,ventilation capacity,gas exchange capacity,and dyspnea symptoms in patients with COPD and may be an independent predictor of AECOPD.

关键词： Oxygen pulse Chronic obstructive pulmonary disease Cardiopulmonary exercise test Acute exacerbation

Luminescent d-f transition Yb(Ⅱ)-containing azacryptates with high photoluminescence quantum yields

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Science China Chemistry 2023年第6期66卷 1750-1757页

作者： Wenchao Yan Zifeng Zhao gang yu Hao Qi Ruoyao Guo Nanlong Zheng Zuqiang Bian Zhiwei Liu Beijing National Laboratory for Molecular Sciences State Key Laboratory of Rare Earth Materials Chemistry and ApplicationsCollege of Chemistry and Molecular EngineeringPeking UniversityBeijing 100871China

Emission tunable d-f transition lanthanide complexes have wide applications in many fields such as lighting and photoredox *** with Ce(Ⅲ)and Eu(Ⅱ)complexes,which have achieved near-unity photoluminescence quantum yields(PLQYs),Yb(Ⅱ)complexes still suffer from low reported PLQYs not exceeding 10%.In this work,two luminescent Yb(Ⅱ)-containing azacryptates YbI_(2)-N8M6(N8M6=4,7,13,16,21,24-hexamethyl-1,4,7,10,13,16,21,24-octaazabicyclo[8.8.8]hexacosane)and YbI2-N8E6(N8E6=4,7,13,16,21,24-hexaethyl-1,4,7,10,13,16,21,24-octaazabicyclo[8.8.8]hexacosane)were synthesized and ***2-N8M6 in solid powder exhibits blue emission with a maximum emission wavelength(λm)of 464 nm and a PLQY of 76%,while YbI_(2)-N8E6 in solid powder exhibits green emission with a λ_(m) of 537 nm and a PLQY up to93%.Moreover,YbI_(2)-N8M6 exhibits mechanochromic property,showing an emission color change from deep-blue to cyan under mechanical *** results will inspire the studies about luminescent Yb(Ⅱ)complexes and their further applications.

关键词： d-f transition Yb(ll)complex luminescence mechanochromic property

Lanthanide complexes with d-f transition:new emitters for single-emitting-layer white organic light-emitting diodes

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光:科学与应用(英文版) 2023年第8期12卷 1623-1632页

作者： Peiyu Fang Peihao Huo Liding Wang Zifeng Zhao gang yu Yanyi Huang Zuqiang Bian Zhiwei Liu Beijing National Laboratory for Molecular Sciences State Key Laboratory of Rare Earth Materials Chemistry and ApplicationsCollege of Chemistry and Molecular EngineeringPeking University100871 BeijingChina

White organic light-emitting diodes(WOLEDs)is a new generation of lighting technology and has stimulated wide-ranging *** the advantage of simple device structure,single-emitting-layer WOLEDs(SEL-WOLEDs)still face the challenges of difficult material screening and fine energy level ***,we report efficient SEL-WOLEDs with a sky-blue emitting cerium(Ⅲ)complex Ce-TBO2Et and an orange-red emitting europium(Ⅱ)complex Eu(Tp2Et)2 as the emitters,showing a maximum external quantum efficiency of 15.9%and Commission Internationale de l'Eclairage coordinates of(0.33,0.39)at various *** importantly,the electroluminescence mechanism of direct hole capture and hindered energy transfer between the two emitters facilitate a manageable weight doping concentration of 5%for Eu(Tp2Et)2,avoiding the low concentration(<1%)of the low-energy emitter in typical *** results indicate that d-f transition emitters may circumvent fine energy level regulation and provide development potential for SEL-WOLEDs.

关键词： emitter diodes transition