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Purification and Characterization of Two Endo-β-1,4-glucanases from Mollusca,Ampullaria crossean

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Acta Biochimica et Biophysica Sinica 2005年第10期37卷 702-708页

作者： Yan-Hong LI~(1#) Rui GUO~(3#) Qiu-yu YIN~1 Ming DING~1 Si-Liang ZHANG~3 Gen-Jun XU~(1,2) Fu-Kun zhao~(1,2*) 1 Key Laboratory of Proteomics,Institute of Biochemistry and Cell Biology,shanghai Institutes for Biological Sciences.Graduate School of the Chinese Academy of Sciences,Chinese Academy of Sciences,shanghai 200031,china 2 college of Life Science,Zhejiang Sci-Tech university,Hangzhou 310018,china 3 Bioengineering Institute,East china university of Science and Technology,shanghai 200233,china KeyLaboratoryofProteomics InstituteofBiochemistryandCellBiologyShanghaiInstitutesforBiologicalSciencesGrad

Two novel endo-β-1,4-glucanases,EG45 and EG27,were isolated from the gastric juice ofmollusca,Ampullaria crossean,by anion exchange,hydrophobic interaction,gel filtration and a secondround of anion exchange *** purified proteins EG45 and EG27 appeared as a single bandon sodium dodecylsulfate polyacrylamide gel electrophoresis with a molecular mass of 45 kDa and 27 kDa,*** optimum pH for CMC activity was 5.5 for EG45 and 4.4-4.8 for *** optimumtemperature range for EG27 was broad,between 50℃ and 60℃;for EG45 it was 50℃.The analysis on thestability of these two endo-β-1,4-glucanases showed that EG27 was acceptably stable at pH 3.0-11.0 evenwhen the incubation time was prolonged to 24 h at 30℃,whereas EG45 remained relatively stable at *** 85% of the activity of EG27 could be retained upon incubation at 60℃ for 24 ***,less than 10% residual activity of EG45 was detected at 50℃.Among different kinds of substrates,bothenzymes showed a high preference for carboxymethyl ***45,in particular,showed a carboxymethylcellulose hydrolytic activity of 146.5 IU/mg *** enzymes showed low activities to xylan (from oatspelt) and Sigmacell 101,and they were inactive to p-nitrophenyl-β-D-cellobioside,salicin and starch.

关键词： cellulase endo-β-1,4-glucanase Ampullaria crossean purification substrate specificity

两步Runge-Kutta法求解延迟微分方程的GPL_m-稳定性(英文)

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系统仿真学报 2011年第7期23卷 1366-1368页

作者：丛玉豪蒋成香上海师范大学数学系上海200234 上海师范大学天华学院上海201815

讨论了两步Runge-Kutta方法求解延迟微分方程的数值稳定性,分析了求解线性试验方程的两步Runge-Kutta方法的稳定性态。证明了两步Runge-Kutta方法是GPLm-稳定的,当且仅当它求解常微分方程是L-稳定的。

关键词：延迟微分方程两步Runge-Kutta方法 GPL-稳定性 L-稳定性

Monitoring Sea Fog over the Yellow Sea and Bohai Bay Based on Deep Convolutional Neural Network

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Journal of Tropical Meteorology 2024年第3期30卷 223-229页

作者： HUANG Bin GAO Shi-bo yu Run-ling zhao Wei ZHOU Guan-bo National Meteorological Center Beijing 100081 China State Key Laboratory of Severe Weather Chinese Academy of Meteorological SciencesBeijing 100081 China Key Laboratory of South china Sea Meteorological Disaster Prevention and Mitigation of Hainan Province Haikou 570203 China Agronomy college Shenyang Agricultural UniversityShenyang 110866 China shanghai Typhoon Institute China Meteorological AdministrationShanghai 200030 China Key Laboratory of Numerical Modeling for Tropical Cyclones China Meteorological AdministrationShanghai 200030 China

In this paper,we utilized the deep convolutional neural network D-LinkNet,a model for semantic segmentation,to analyze the Himawari-8 satellite data captured from 16 channels at a spatial resolution of 0.5 km,with a focus on the area over the Yellow Sea and the Bohai Sea(32°-42°N,117°-127°E).The objective was to develop an algorithm for fusing and segmenting multi-channel images from geostationary meteorological satellites,specifically for monitoring sea fog in this ***,the extreme gradient boosting algorithm was adopted to evaluate the data from the 16 channels of the Himawari-8 satellite for sea fog detection,and we found that the top three channels in order of importance were channels 3,4,and 14,which were fused into false color daytime images,while channels 7,13,and 15 were fused into false color nighttime ***,the simple linear iterative super-pixel clustering algorithm was used for the pixel-level segmentation of false color images,and based on super-pixel blocks,manual sea-fog annotation was performed to obtain fine-grained annotation *** deep convolutional neural network D-LinkNet was built on the ResNet backbone and the dilated convolutional layers with direct connections were added in the central part to form a string-and-combine structure with five branches having different depths and receptive *** show that the accuracy rate of fog area(proportion of detected real fog to detected fog)was 66.5%,the recognition rate of fog zone(proportion of detected real fog to real fog or cloud cover)was 51.9%,and the detection accuracy rate(proportion of samples detected correctly to total samples)was 93.2%.

关键词： deep convolutional neural network satellite images sea fog detection multi-channel image fusion

海马注射β-淀粉样蛋白前体蛋白抗体诱导神经元的退行性变及学习记忆障碍(英文)

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生物化学与生物物理进展 2011年第10期38卷 908-918页

作者：许玉霞王洪权赵红郭景春朱粹青复旦大学上海医学院医学神经生物学国家重点实验室上海200032

探讨海马内注射淀粉样蛋白前体蛋白(APP)抗体诱导细胞表面APP的铰链是否影响大鼠的水迷宫行为学以及是否诱导神经元的退行性改变,并进一步探讨其可能的机制.成年雄性SD大鼠海马内分别注射生理盐水、对照IgG和APP抗体.水迷宫行为学检测... 详细信息

探讨海马内注射淀粉样蛋白前体蛋白(APP)抗体诱导细胞表面APP的铰链是否影响大鼠的水迷宫行为学以及是否诱导神经元的退行性改变,并进一步探讨其可能的机制.成年雄性SD大鼠海马内分别注射生理盐水、对照IgG和APP抗体.水迷宫行为学检测测试动物的学习和记忆能力.Cresyl Violet(CV)和Fluoro-Jade B染色观察神经元的退行性变.免疫组织化学方法检测MAP-2和磷酸化paxillin及磷酸化tau蛋白在海马的异常表达和分布.海马内注射APP抗体可延长动物的寻台潜伏期,减少大鼠在平台所在象限的探索时间和穿梭次数.CV和Fluoro Jade-B染色结果显示,海马注射APP抗体可导致海马锥体细胞的死亡和退变.同时伴MAP2免疫染色的减少和磷酸化paxillin及磷酸化tau的免疫染色的增加.上述结果表明,海马内注射APP抗体可诱导学习和记忆功能障碍及神经元的退行性改变,其机制可能与MAP-2和磷酸化paxillin及磷酸化tau的异常表达分布有关.

关键词：淀粉样蛋白前体蛋白水迷宫神经元退行性变海马 paxillin tau

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α集团结构在相对论重离子碰撞中的效应

中国科学：物理学、力学、天文学 2024年第9期54卷 30-45页

作者：马余刚张松复旦大学核科学与技术系/现代物理研究所上海200433 国家自然科学基金委理论物理专款-上海核物理理论研究中心上海200433

相对论重离子碰撞的主要物理目标是寻找夸克-胶子等离子体(QGP)物质状态,并研究其性质.在开展相对论重离子碰撞物理研究的数十年中,人们在实验和理论上提出了各类探针来探测QGP信号,并通过这些观测量研究QGP的性质,集体流是其中最重要... 详细信息

相对论重离子碰撞的主要物理目标是寻找夸克-胶子等离子体(QGP)物质状态,并研究其性质.在开展相对论重离子碰撞物理研究的数十年中,人们在实验和理论上提出了各类探针来探测QGP信号,并通过这些观测量研究QGP的性质,集体流是其中最重要的探针之一.相对论流体力学对集体流的描述表明,非对心碰撞区域形成的火球在向外膨胀过程中,动量空间逐渐呈现各向异性的分布,这一分布特性直接受到碰撞初态位置空间的影响.近些年,随着研究的深入,人们关注到了一些引人深思的问题,如对心碰撞中是否存在椭圆流的信号?原子核的初态形变对末态动量空间产生怎样的影响?特殊核结构是否在集体流等观测量中呈现可辨认的信号?针对这些问题,国际上已经开展了一些研究工作,而且部分的理论研究工作已催生了新的实验测量.据此,一方面有必要对这些研究历程进行梳理,供今后的研究参考.另一方面,有必要讨论通过相对论重离子碰撞研究核结构问题的可能性.本文以α集团结构为例,对上述研究进行一个回顾,并对高能核-核碰撞作为核结构研究的新途径进行探讨.

关键词：相对论重离子碰撞夸克-胶子等离子体集体流椭圆流三角流 α集团

Nano-bio interactions between 2D nanomaterials and mononuclear phagocyte system cells

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BMEMat(Biomedical Engineering Materials) 2024年第2期2卷 92-117页

作者： Jing zhao Zheng Chen Shanbiao Liu Pin Li Shiyi yu Daishun Ling Fangyuan Li Institute of Pharmaceutics Hangzhou Institute of Innovative MedicineCollege of Pharmaceutical SciencesZhejiang UniversityHangzhouChina Frontiers Science Center for Transformative Molecules School of Chemistry and Chemical EngineeringSchool of Biomedical EngineeringNational Center for Translational MedicineShanghai Jiao Tong UniversityShanghaiChina World Laureates Association(WLA)Laboratories ShanghaiChina Key Laboratory of Precision Diagnosis and Treatment for Hepatobiliary and Pancreatic Tumor of Zhejiang Province HangzhouChina Songjiang Institute and Songjiang Hospital Shanghai Jiao Tong University School of MedicineShanghaiChina

Two-dimensional(2D)nanomaterials,known for their unique atomic arrangements and exceptional physicochemical properties,have garnered significant attention in biomedical applications,particularly in the realms of immunotherapy for tissue engineering and tumor *** applications necessitate a thorough assessment of the potential influence of 2D nanomaterials on immune ***,the mononuclear phagocyte system(MPS)cells,which play pivotal roles in both innate and adaptive immunity,are essential for maintaining organismal *** cells with phagocytic capability contribute to the prevention of foreign body invasion and the elimination of dead or senescent ***,MPS cells,including macrophages and dendritic cells,serve as vital bridges between innate and adaptive immune ***,understanding the nano-bio interactions between 2D nanomaterials and MPS cells is *** nano-bio interactions including cellular uptake,cytocompatibility,and immunological impact are invaluable forthe purposeful design of 2D ***,we provide an overview of the latest advancements in understanding the nano-bio interactions between 2D nanomaterials and MPS cells,and discuss the current challenges and future prospects of employing 2D nanomaterials in the field of nanomedicine.

关键词： 2D nanomaterials cellular uptake dendritic cells immunological impact macrophages mononuclear phagocyte system nano-bio interactions

Gradient layered MXene/Fe_(3)O_(4)@CNTs/TOCNF ultrathin nanocomposite paper exhibiting effective electromagnetic shielding and multifunctionality

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Nano Research 2024年第9期17卷 8233-8242页

作者： Zhengkun Ma Jingzong He Shilin Liu Xiaoxuan Qie Meiheng gan Ruonan Cheng Qilin Wu Malcolm xing State Key Laboratory for Modification of Chemical Fibers and Polymer Materials College of Materials Science and EngineeringDonghua UniversityShanghai 201620China college of Chemistry and Chemical Engineering Donghua UniversityShanghai 201620China Department of Mechanical Engineering University of ManitobaWinnipegMB R3T 2N2Canada

As wearable electronic devices are rapidly developing,there is an urgent need for lightweight,flexible,and ultrathin multifunctional electromagnetic interference(EMI)shielding ***,the flexible ultrathin paper that combines efficient shielding and multifunctional integration remains a considerable ***,a novel MXene/Fe_(3)O_(4)@CNTs/TOCNF(MCT,MXene=transition metal carbide/carbonitride,CNTs=carbon nanotubes,TOCNF=TEMPO-oxidized cellulose nanofiber,TEMPO=2,2,6,6-tetramethylpiperidine-1-oxyl radical)nanocomposite paper with a multilayer electromagnetic gradient structure and electromagnetic dual losses was successfully prepared by a simple filtration *** from effective gradient design and adjusting the proportion of TOCNF,the composite paper(only 18μm)exhibits outstanding shielding effectiveness(SE)of 66 dB in the X-band,ultrahigh thickness-specific SE and surface-specific SE values of 3300 dB·mm-1 and 31,428 dB·cm^(2)·g^(-1)***,dehydroxylation treatment improves MCT paper's hydrophobicity,environmental stability,and mechanical strength,expanding its range of ***,the highly efficient Joule heating properties and hydrophobicity provide MCT additional de-icing *** also simulated the electromagnetic shielding effects of MCT composite paper,which was applied in *** study documents an innovative and intriguing material combination,providing a simple and effective manufacturing strategy for developing EMI shielding *** paper is highly suitable for outdoor portable or wearable electronic devices and has significant application potential in humid/severe cold environments.

关键词： cellulose fiber thin papers nanocomposites electromagnetic interference(EMI)shielding

AN INVESTIGATION INTO IMPINGEMENT JETS IN CYLINDRICAL CHAMBER

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Journal of Hydrodynamics 1991年第1期3卷 90-94页

作者： yu Jie Wu Min-quan Dai gan-ce Zhang Li, UNILAB Research Center of Chemical Reaction Engineering, East china university of Chemical Technolvgy, shanghai 200237, P. R. china East china Univ of Chemical Technology Shanghai China

Laser Doppler Anemometer (LDA) is used for studying the effects of various parameters of impingement jets in cylindrical chamber (IJCC), such as d/D, H/d, γ (momentum ratio) and Re, on flow pattern, as well as those on turbulence intensity. Experimental results show that turbulence intensity is enhanced with decrease of II/d and/ or increase of d/D; and turbulence intensity approaches to a maximum as T=1. Re imposes a quite complicated in- fluence. In case the jet velocity remains unchanged, turbulence intensities might keep almost constant when Re's vary even a magnitude of order.

关键词： Re AN INVESTIGATION INTO IMPINGEMENT JETS IN CYLINDRICAL CHAMBER

兴奋性氨基酸受体的激活介导冷水应激诱导的tau蛋白磷酸化(英文)

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生物化学与生物物理进展 2010年第5期37卷 510-516页

作者：武凤英冯琼程敏闫洁许玉霞朱粹青复旦大学上海医学院医学神经生物学国家重点实验室上海200032

为探讨兴奋性神经传递系统是否参与冷水应激引起的tau蛋白磷酸化,将小鼠于4℃冷水应激5min.采用免疫印迹和免疫组织化学方法分析应激后脑内c-fos和磷酸化tau蛋白的表达情况;运用HPLC检测冷水应激后小鼠脑内兴奋性或抑制性神经递质的变化... 详细信息

为探讨兴奋性神经传递系统是否参与冷水应激引起的tau蛋白磷酸化,将小鼠于4℃冷水应激5min.采用免疫印迹和免疫组织化学方法分析应激后脑内c-fos和磷酸化tau蛋白的表达情况;运用HPLC检测冷水应激后小鼠脑内兴奋性或抑制性神经递质的变化;同时分析兴奋性氨基酸受体和L-型钙通道拮抗剂预处理后冷水应激小鼠脑内磷酸化tau蛋白的水平.冷水应激后1h,海马内磷酸化tau蛋白的水平显著升高,同时伴c-fos的染色增加.HPLC检测显示,兴奋性和抑制性神经递质呈现急剧上升而后又下降的趋势.冷水应激后15min,天冬氨酸和甘氨酸水平显著升高,1h后天冬氨酸、谷氨酸、牛磺酸和γ-氨基丁酸显著下降.NMDA受体拮抗剂MK-801(5mg/kg)和AMPA受体拮抗剂DNQX(0.5,5mg/kg)可显著抑制冷水应激引起的磷酸化tau蛋白水平的升高,代谢性谷氨酸受体拮抗剂MAP-4不影响tau蛋白的磷酸化,另外,L-型钙通道阻断剂尼莫地平可抑制冷水应激引起的磷酸化tau蛋白水平的升高.这些结果表明,冷水应激可影响兴奋性神经传递系统,通过离子型兴奋性氨基酸受体和异常神经激活来调节tau蛋白的磷酸化.兴奋性神经传递系统的激活在冷水应激诱导的海马tau蛋白的磷酸化中发挥作用.

关键词：冷水应激 Tau蛋白磷酸化兴奋性氨基酸神经递质 HPLC