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World Journal of Gastroenterology 2005年第29期11卷 4596-4599页

作者： yu fan shu zheng ze-feng xu jia-yi ding Cancer Institute 2^nd Affiliated Hospital School of Medicine Zhejiang University Hangzhou 310009 Zhejiang Province China

AIM： To investigate the effects of polo-like kinase-1 （PLK1） antisense phosphorothioate oligodeoxynucleotide （ASODN） on apoptosis and cell cycle of human colon cancer cell line SW480. METHODS： After SW480 colon cancer cells were transfected with PLK1 ASODN, Northern and Western blot analyses were used to examine PLK1 gene expression in cancer cells. We studied apoptosis using terminal uridine deoxynucleotidyl nick end labeling. Apoptosis and cell cycle of SW480 cells were examined by fluorescence-activated cell sorter scan. RESULTS： The levels of PLK1 mRNA and protein were greatly inhibited by PLK1 ASODN in SW480 cancer cells transfected with PLK1 ASODN. Apoptosis index （AI） induced PLK1 ASODN in a time- and dose-dependent manner. Results from FLM showed that sub-2N DNA content of transfected cancer cells was significantly increased and arrested at G2/M compared with control groups. CONCLUSION： PLK1 ASODN can induce apoptosis of human colon cancer cell line SW480.

关键词： Polo-like kinase-1 Antisense Apoptosis Cell cycle

Genome-wide analysis of OCT4 binding sites in glioblastoma cancer cells

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2011年第10期12卷 812-819页

作者： xue-feng fan Wei-yi ZHANG Na ZHAO Wei yu Dong ding xu HONG Li-sha LI Hua-rong ZHANG shu zheng Biao-yang LIN Key Laboratory of Cancer Prevention and Intervention of Ministry of Education Key Laboratory of Molecular Biology in Medical Sciences of Zhefiang Province Cancer Institute the Second Affiliated Hospital School of Medicine Zhejiang University Hangzhou 310009 China Systems Biology Div＆ion Zhejiang-California International Nanosystems Institute （ZCNI） Zhejiang University Hangzhou 310029 China

OCT4, a member of the POU family of gene products, is an octamer motif-binding transcription factor. As it is known to play a crucial role in cancer processes including proliferation, invasion, and chemoradioresistance, it is important to identify the direct targets of OCT4 in living cancer cells. Here, chromatin immunoprecipitation-sequencing （ChlP-seq） was used to identify OCT4 binding sites in glioblastoma cancer cells. The results showed that 5438 OCT4 binding sites were localized in the glioblastoma cancer genome and that these sites contained a consensus sequence TTTkswTw （k=T or G, s=C or G, w=A or T）, which occurred 3931 times in 2312 OCT4 binding regions. Furthermore, binding motifs of some other transcription factors were identified in OCT4 binding regions. Our results provide a valuable dataset for understanding gene regulation mechanisms underlying the function of OCT4 in glioblastoma cancer.

关键词： OCT4 Chromatin immunoprecipitation-sequencing (ChlP-seq) DNA binding region Glioblastoma

Construction of RuO_(2)-Ru/MoO_(2)@carbon cloth bifunctional electrocatalyst for efficient overall water splitting

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Rare Metals 2024年第10期43卷 5095-5104页

作者： jia-Lin Cai Jing-yi fan xu-Dong Zhang Xin Xie Wan-yu Tian Xin-Gang Zhang Jie ding yu-Shan Liu College of Material Henan Key Laboratory of Aeronautical Materials and Application TechnologyZhengzhou University of AeronauticsZhengzhou 450046China The Green Catalysis Center and College of ChemistryZhengzhou UniversityZhengzhou 450000China

Efficient bifunction electrocatalyst is extremely interesting for electrochemical overall water splitting(OWS).Herein,a new RuO_(2)-Ru/MoO_(2)@CC(RRM/CC)bifunctional electrocatalyst was prepared via a solid phase reaction *** obtain a suitable precursor for SPR,MoS_(2)nanosheets and RuO_(2)nanoparticles(NPs)were sequentially loaded onto carbon cloth conductive ***,the prepared RuO_(2)/MoS_(2)/CC precursor was sealed in a furnace and annealed in Ar to trigger the redox *** SPR,active RuO_(2)-Ru/MoO_(2)units containing metal–metal oxide interfaces were formed on CC substrate *** optimized RRM/CC sample annealed at 400℃exhibited a overpotential of 13 m V for hydrogen evolution reaction(HER)and 231 mV for oxygen evolution reaction(OER)at 10 mA·cm^(-2)under alkaline condition,respectively,which can be deduced to the modulated electronic structure and unique hierarchica *** addition,a low cell voltage of 1.48 V for OWS was required at 10 mA·cm^(-2)under alkaline condition Meanwhile,RRM/CC exhibited excellent pH-independent durability.

关键词： Ru RuO_(2) Bifunctional Electrocatalyst Overall water splitting

Tanshinone ⅡA prevents acute lung injury by regulating macrophage polarization

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Journal of Integrative Medicine 2022年第3期20卷 274-280页

作者： jia-yi Zhao Jin Pu jian fan Xin-yu feng jian-wen xu Rong Zhang Yan Shang Department of General Practice Changhai HospitalNaval Medical UniversityShanghai 200433China Department of General Practice Changhai Hongkou HospitalShanghai 200081China Department of Special Clinic Changhai HospitalNaval Medical UniversityShanghai 200433China Shanghai Institute of Biochemical and Cell biology Chinese Academy of SciencesShanghai 200031China Department of Nursing Shidong HospitalUniversity of Shanghai for Science and TechnologyShanghai 200438China Department of Respiratory and Critical Care Medicine Changhai HospitalNaval Medical UniversityShanghai 200433China

Objective: Acute lung injury(ALI) is a serious respiratory dysfunction caused by pathogen or physical invasion. The strong induced inflammation often causes death. Tanshinone ⅡA(Tan-ⅡA) is the major constituent of Salvia miltiorrhiza Bunge and has been shown to display anti-inflammatory effects. The aim of the current study was to investigate the effects of Tan-ⅡA on ***: A murine model of lipopolysaccharide(LPS)-induced ALI was used. The lungs and serum samples of mice were extracted at 3 days after treatment. ALI-induced inflammatory damages were confirmed from cytokine detections and histomorphology observations. Effects of Tan-ⅡA were investigated using in vivo and in vitro ALI models. Tan-ⅡA mechanisms were investigated by performing Western blot and flow cytometry experiments. A wound-healing assay was performed to confirm the Tan-ⅡA ***: The cytokine storm induced by LPS treatment was detected at 3 days after LPS treatment, and alveolar epithelial damage and lymphocyte aggregation were observed. Tan-ⅡA treatment attenuated the LPS-induced inflammation and reduced the levels of inflammatory cytokines released not only by inhibiting neutrophils, but also by macrophage. Moreover, we found that macrophage activation and polarization after LPS treatment were abrogated after applying the Tan-ⅡA treatment. An in vitro assay also confirmed that including the Tan-ⅡA supplement increased the relative amount of the M2 subtype and decreased that of M1. Rebalanced macrophages and Tan-ⅡA inhibited activations of the nuclear factor-κB and hypoxia-inducible factor pathways. Including Tan-ⅡA and macrophages also improved alveolar epithelial repair by regulating macrophage ***: This study found that while an LPS-induced cytokine storm exacerbated ALI, including Tan-ⅡA could prevent ALI-induced inflammation and improve the alveolar epithelial repair, and do so by regulating macrophage polarization.

关键词： Acute lung injury Inflammation Cytokine storm TanshinoneⅡA Macrophages

L-Type Calcium Channel Modulates Low-Intensity Pulsed Ultrasound-Induced Excitation in Cultured Hippocampal Neurons

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Neuroscience Bulletin 2024年第7期40卷 921-936页

作者： Wen-Yong fan yi-Ming Chen yi-fan Wang yu-Qi Wang jia-Qi Hu Wen-xu Tang yi feng Qian Cheng Lei xue State Key Laboratory of Medical Neurobiology and MOE Frontiers Center for Brain Science Fudan UniversityShanghai 200433China Department of Physiology and Neurobiology School of Life SciencesFudan UniversityShanghai 200438China Institute of Acoustics School of Physics Science and EngineeringTongji UniversityShanghai 200092China Key Laboratory of Spine and Spinal Cord Injury Repair and Regeneration of Ministry of Education Tongji UniversityShanghai 200070China Center for Rehabilitation Medicine Department of Pain ManagementZhejiang Provincial People's HospitalAffliated People's HospitalHangzhou Medical CollegeHangzhou 310014China Department of Critical Care Medicine Shanghai General HospitalShanghai Jiaotong University School of MedicineShanghai 200080China Shanghai Research Institute for Intelligent Autonomous Systems Tongji UniversityShanghai 201210China Research Institute of Intelligent Complex Systems Fudan UniversityShanghai 200433China

As a noninvasive technique,ultrasound stimulation is known to modulate neuronal activity both in vitro and in *** latest explanation of this phenomenon is that the acoustic wave can activate the ion channels and further impact the electrophysiological properties of targeted ***,the underlying mechanism of low-intensity pulsed ultrasound(LIPUS)-induced neuro-modulation effects is still ***,we characterize the excitatory effects of LIPUS on spontaneous activity and the intracellular Ca^(2+)homeostasis in cultured hippocampal *** whole-cell patch clamp recording,we found that 15 min of 1-MHz LIPUS boosts the frequency of both spontaneous action potentials and spontaneous excitatory synaptic currents(sEPSCs)and also increases the amplitude of sEPSCs in hippocampal *** phenomenon lasts for>10 min after LIPUS *** with Ca^(2+)imaging,we clarified that LIPUS increases the[Ca^(2+)]cyto level by facilitating L-type Ca^(2+)channels(LTCCs).In addition,due to the[Ca^(2+)]cyto elevation by LIPUS exposure,the Ca^(2+)-dependent CaMKII-CREB pathway can be activated within 30 min to further regulate the gene transcription and protein *** work suggests that LIPUS regulates neuronal activity in a Ca^(2+)-dependent manner via *** may also explain the multi-activation effects of LIPUS beyond *** stimulation potentiates spontaneous neuronal activity by increasing Ca^(2+)influx.

关键词： Low-intensity pulsed ultrasound Neuromodulation L-type calcium channel Hippocampal neuron

Anterior abdominal abscess-a rare manifestation of severe acute pancreatitis:A case report

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World Journal of Clinical Cases 2021年第30期9卷 9218-9227页

作者： yu-Chen jia yi-xuan ding Wen-Tong Mei Zhi-Gang xue Zhi zheng yuan-xu Qu jia Li feng Cao Fei Li Department of General Surgery Xuanwu HospitalCapital Medical UniversityBeijing 100053China Clinical Center for Acute Pancreatitis Capital Medical UniversityBeijing 100053China

BACKGROUND Severe acute pancreatitis(SAP)is a common critical disease of the digestive *** addition to the clinical manifestations and biochemical changes of acute pancreatitis,SAP is also accompanied by organ failure lasting more than 48 *** is characterized by focal or extensive pancreatic necrosis,hemorrhage and obvious inflammation around the *** peripancreatic fat space,fascia,mesentery and adjacent organs are often *** common local complications include acute peripancreatic fluid collection,acute necrotic collection,pancreatic pseudocyst,walled off necrosis and infected pancreatic *** reviewing the literature,we found that in very few cases,SAP patients have complications with anterior abdominal wall *** SUMMARY We report a 66-year-old Asian male with severe acute pancreatitis who presented with intermittent abdominal pain and an increasing abdominal *** abscess spread from the retroperitoneum to the anterior abdominal wall and the right *** the described case,drainage tubes were placed in the retroperitoneal and anterior abdominal wall by percutaneous *** a series of symptomatic supportive therapies,the patient was discharged from the hospital with a retroperi-toneal drainage tube after the toleration of oral feeding and the improvement of nutritional *** We believe that patients with SAP complicated with anterior abdominal abscess can be treated conservatively to avoid unnecessary exploration or operation.

关键词： Severe acute pancreatitis Abdominal abscess Complication Drainage Case report

Promise and challenges of traditional Chinese medicine,specifically Calculus bovis,in liver cancer treatment

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World Journal of Gastroenterology 2024年第40期30卷 4380-4385页

作者： Ao-Xi xu Zhi-feng Zhao Li Zhu yi-Heng Zhang Yan Li yu-fan Wei Bo-Ya Zhang Bin jiang Tian-ze Gao Meng-Si Li jia-yu Liu School of Medicine Nankai UniversityTianjin 300071China Department of General Surgery The First Medical Center of Chinese People's Liberation Army General HospitalBeijing 100853China Medical School of Chinese People's Liberation Army Medical School of Chinese People's Liberation ArmyBeijing 100853China Department of Hepatobiliary and Pancreative Surgery The First Medical CenterChinese People's Liberation Army General HospitalBeijing 100853China Department of Surgery Mancheng District People's HospitalBaoding 072150Hebei ProvinceChina Department of Neurosurgery First Medical Centre of Chinese People's Liberation Army General HospitalBeijing 100853China

Liver cancer,one of the most common malignancies worldwide,ranks sixth in incidence and third in *** cancer treatment options are diverse,inclu-ding surgical resection,liver transplantation,percutaneous ablation,transarterial chemoembolization,radiotherapy,chemotherapy,targeted therapy,immuno-therapy,and traditional Chinese medicine(TCM).A multidisciplinary team(MDT)is essential to customize treatment plans based on tumor staging,liver function,and performance status(PS),ensuring individualized patient *** decisions require a MDT to tailor strategies based on tumor staging,liver function,and PS,ensuring personalized *** approval of new first-line and second-line drugs and the establishment of standard treatments based on immune checkpoint inhibitors have significantly expanded treatment options for advanced liver cancer,improving overall ***,many patients do not respond effectively to these treatments and ultimately succumb to the *** oncology treatments,while extending patient survival,often come with severe side effects,resistance,and damage to the body,negatively impacting quality of *** et al's study published at World Journal of Gastroenterology rigorously validates the anticancer properties of Calculus bovis,enhancing our understanding of TCM and contributing to new liver cancer treatment *** over 5000 years,TCM has been used in East Asian countries like China to treat various diseases,including liver *** of real-world clinical data suggests that for patients with advanced-stage tumors lacking effective treatments,integrated TCM therapies could provide significant breakthroughs.

关键词： Traditional Chinese medicine Liver cancer Calculus bovis Treatment Promise Challenges

Effective transport network driven by tortuosity gradient enables high-electrochem-active solid-state batteries

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National Science Review 2023年第3期10卷 178-188页

作者： Qing-Song Liu Han-Wen An xu-feng Wang fan-Peng Kong Ye-Cai Sun yu-Xin Gong shuai-feng Lou yi-fan Shi Nan Sun Biao Deng jian Wang jia-Jun Wang Ministry of Industry and Information Technology(MIIT) Key Laboratory of Critical Materials Technology for New Energy Conversion and Storage School of Chemistry and Chemical EngineeringHarbin Institute of Technology(HIT) Chongqing Research Institute of HIT Shanghai Institute of Applied Physics Chinese Academy of Sciences Canadian Light Source Inc. University of Saskatchewan

Simultaneously achieving high electro chemical activity and high loading for solid-state batteries has been hindered by slow ion transport within solid electrodes,in particular with an increase in electrode *** transport governed by ’point-to-point’ diffusion inside a solid-state electrode is challenging,but still remains ***,synchronized electrochemical analysis using X-ray tomography and ptychography reve als new insights into the nature of slow ion transport in solid-state electrodes.’hickness-dependent delithiation kinetics are spatially probed to identify that low-delithiation kinetics originate from the high tortuous and slow longitudinal transport *** fabricating a tortuosity-gradient electrode to create an effective ion-percolation network,the tortuosity-gradient electrode architecture promotes fast charge transport,migrates the heterogeneous solid-state reaction,enhances electrochemical activity and extends cycle life in thick solid-state ele *** findings establish effective transport pathways as key design principles for realizing the promise of solid-state high-loading cathodes.

关键词： all-solid-state batteries thick electrodes lithiation/delithiation heterogeneity tortuosity gradient synchrotron techniques

Mesenchymal stem cell-derived extracellular vesicles in skin wound healing:roles,opportunities and challenges

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Military Medical Research 2024年第5期11卷 400-429页

作者： jia-yi ding Min-jiang Chen Ling-feng Wu Gao-feng shu Shi-Ji fang Zhao-yu Li xu-Ran Chu Xiao-Kun Li Zhou-Guang Wang jian-Song Ji Key Laboratory of Imaging Diagnosis and Minimally Invasive Intervention Research Institute of Imaging Diagnosis and Minimally Invasive Intervention Researchthe Fifth Affiliated Hospital of Wenzhou Medical UniversityZhejiangLishui 323000China Oujiang Laboratory(Zhejiang Lab for Regenerative Medicine Vision and Brain Health)School of Pharmaceutical ScienceWenzhou Medical UniversityWenzhou 325035ZhejiangChina Clinical College of the Affiliated Central Hospital School of MedicineLishui UniversityLishui 323000ZhejiangChina Department of Overseas Education College Jimei UniversityXiamen 361021FujianChina Department of Medicine Ⅱ Internal MedicineCardio-Pulmonary Institute(CPI)Universities of Giessen and Marburg Lung Center(UGMLC)Member of the German Center for Lung Research(DZL)Justus-Liebig University Giessen35392 GiessenGermany Pulmonary and Critical Care Department of Medicine VInternal MedicineInfectious Diseases and Infection ControlUniversities of Giessen and Marburg Lung Center(UGMLC)Member of the German Center for Lung Research(DZL)Justus-Liebig University Giessen35392 GiessenGermany

Skin wounds are characterized by injury to the skin due to trauma,tearing,cuts,or *** such injuries are common to all human groups,they may at times represent a serious socioeconomic ***,increasing numbers of studies have focused on the role of mesenchymal stem cell(MSC)-derived extracellular vesicles(EVs)in skin wound *** a cell-free therapy,MSC-derived EVs have shown significant application potential in the field of wound repair as a more stable and safer option than conventional cell *** based on MSC-derived EVs can significantly promote the repair of damaged substructures,including the regeneration of vessels,nerves,and hair *** addition,MSC-derived EVs can inhibit scar formation by affecting angiogenesis-related and antifibrotic pathways in promoting macrophage polarization,wound angiogenesis,cell proliferation,and cell migration,and by inhibiting excessive extracellular matrix ***,these structures can serve as a scaffold for components used in wound repair,and they can be developed into bioengineered EVs to support trauma *** the formulation of standardized culture,isolation,purification,and drug delivery strategies,exploration of the detailed mechanism of EVs will allow them to be used as clinical treatments for wound *** conclusion,MSCderived EV-based therapies have important application prospects in wound *** we provide a comprehensive overview of their current status,application potential,and associated drawbacks.

关键词： Mesenchymal stem cell(MSC) Extracellular vesicles(EVs) Wound repair Engineered nanoparticles

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VX-509 attenuates the stemness characteristics of colorectal cancer stem-like cells by regulating the epithelial-mesenchymal transition through Nodal/Smad2/3 signaling

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World Journal of Stem Cells 2024年第2期16卷 207-227页

作者： yun yuan xu-fan Zhang yu-Chen Li Hong-Qing Chen Tian Wen jia-Lian zheng Zi-yi Zhao Qiong-ying Hu Department of Laboratory Medicine Hospital of Chengdu University of Traditional Chinese MedicineChengdu 610072Sichuan ProvinceChina Department of Nuclear Medicine Hospital of Chengdu University of Traditional Chinese MedicineChengdu 610072Sichuan ProvinceChina College of Medical Technology Chengdu University of Traditional Chinese MedicineChengdu 611137Sichuan ProvinceChina Department of Hepatology Affiliated Hospital of Liaoning University of Traditional Chinese MedicineShenyang 110032Liaoning ProvinceChina Hospital of Chengdu University of Traditional Chinese Medicine Chengdu 610072Sichuan ProvinceChina Traditional Chinese Medicine Regulating Metabolic Diseases Key Laboratory of Sichuan Province Chengdu 610072Sichuan ProvinceChina

BACKGROUND Colorectal cancer stem cells(CCSCs)are heterogeneous cells that can self-renew and undergo multidirectional differentiation in colorectal cancer(CRC)*** are generally accepted to be important sources of CRC and are responsible for the progression,metastasis,and therapeutic resistance of ***,targeting this specific subpopulation has been recognized as a promising strategy for overcoming *** To investigate the effect of VX-509 on CCSCs and elucidate the underlying *** CCSCs were enriched from CRC cell lines by in conditioned serum-free *** blot,Aldefluor,transwell and tumorigenesis assays were performed to verify the phenotypic characteristics of the *** anticancer efficacy of VX-509 was assessed in HCT116 CCSCs and HT29 CCSCs by performing cell viability analysis,colony formation,sphere formation,flow cytometry,and western blotting assessments in vitro and tumor growth,immunohistochemistry and immunofluorescence assessments in *** Compared with parental cells,sphere cells derived from HCT116 and HT29 cells presented increased expression of stem cell transcription factors and stem cell markers and were more potent at promoting migration and tumori-genesis,demonstrating that the CRC sphere cells displayed CSC ***-509 inhibited the tumor malignant biological behavior of CRC-stem-like cells,as indicated by their proliferation,migration and clonality in vitro,and suppressed the tumor of CCSC-derived xenograft tumors in ***,VX-509 suppressed the CSC character-istics of CRC-stem-like cells and inhibited the progression of epithelial-mesenchymal transition(EMT)signaling in *** was identified as the regulatory factor of VX-509 on CRC stem-like cells through analyses of differen-tially expressed genes and CSC-related database ***-509 markedly downregulated the expression of Nodal and its downstream phosphorylated Smad2/3 to inhibit EMT ***,VX-509 rev

关键词： Colorectal cancer stem cells Stemness VX-509 Epithelial-mesenchymal transition Nodal