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Determination of ppt Level Chromium(vi) Using the Gold Nano-Flakes Electrodeposited on Platinum Rotating Disk Electrode and Modified with 4-thiopyridinium

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American Journal of Analytical Chemistry 2015年第5期6卷 457-467页

作者： Huy Du Nguyen t. thuy Luyen Nguyen Khac Manh Nguyen t. Anh tuyet tran Anh Mai Nguyen Quoc Hien Nguyen Central Laboratory for Analysis University of Science Vietnam National University in Ho Chi Minh City Ho Chi Minh City Vietnam Research and Development Center for Radiation technology Vietnam Atomic Energy Institute Ho Chi Minh City Vietnam

the nano-gold layer formed on the platinum rotating disk electrode (nano-Au/Pt-RD) inherited the catalytic property for Cr(vi) reduction from platinum surface and owned the good features of nano-gold such as insensitivity with hydrogen ion, high surface area, augmenting diffusion of Cr(vi) and ability for self-assembling with 4-pyridine-ethanethiol (PEt) through Au←S linkages, to form PEt/nano-Au/Pt-RD electrode capable of accumulating Cr(vi) from sample. the obtained PEt/ nano-Au/Pt-RD electrode showed an extreme sensitivity to Cr(vi). By using this electrode, 1.09 ng·L﹣1 was the detection limit of differential pulse adsorptive cathodic stripping voltammetry for Cr(vi) with the accumulation time of only 2 min. Moreover, this electrode was reproducible with 3.5% RSD for 30 times of Cr(vi) accumulating and stripping. In addition, this electrode was also selective for Cr(vi) determination, which was not almost interfered by other inorganic ions.

关键词： Nano-Gold Layer Platinum Electrode Stripping Voltammetry

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Intrahepatic expression of genes related to metabotropic receptors in chronic hepatitis

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World Journal of Gastroenterology 2012年第31期18卷 4156-4161页

作者： Andrzej Ciesla Maciej Kusmider Agata Faron-Górecka marta Dziedzicka-Wasylewska Monika Bocisga-Jasik Danuta Owczarek Irena Cieko-Michalska Dorota Cibor tomasz Mach Department of Gastroenterology Hepatology and Infectious DiseasesJagiellonian University Medical College31-521 KrakówPoland Institute of Pharmacology Polish Academy of Sciences31-343 KrakówPoland

AIM： to screen for genes related to metabotropic re- ceptors that might be involved in the development of chronic hepatitis. MEtHODS： Assessment of 20 genes associated with metabotropic receptors was performed in liver speci- mens obtained by punch biopsy from 12 patients with autoimmune and chronic hepatitis type B and C. For this purpose, a microarray with low integrity grade and with oligonucleotide DNA probes complementary to target transcripts was used. Evaluation of gene expression was performed in relation to transcript level, correlation between samples and grouping of clinical parameters used in chronic hepatitis assessment. Clini- cal markers of chronic hepatitis included alanine and aspartate aminotransferase, ~,-glutamyltranspeptidase, alkaline phosphatase and cholinesterase activity, levels of iron ions, total cholesterol, triglycerides, albumin, glucose, hemoglobin, platelets, histological analysis of inflammatory and necrotic status, fibrosis according to MEtAviR score, steatosis, as well as anthropometric body mass index, waist/hip index, percentage of adi- pose tissue and liver size in ultrasound examination. Gender, age, concomitant diseases and drugs were also taken into account. Validation of oligonucleotide microarray gene expression results was done with the use of quantitative real-time polymerase chain reaction （qRt-PCR）. RESULtS： the highest （0.002 〈 P 〈 0.046） expres- sion among genes encoding main components of metabotropic receptor pathways, such as the a subunit of G-coupled protein, phosphoinositol-dependent pro- tein kinase or arrestin was comparable to that of an- giotensinogen synthesized in the liver. Carcinogenesis suppressor genes, such as chemokine ligand 4, tran- scription factor early growth response protein 1 and lysophosphatidic acid receptor, were characterized by the lowest expression （0.002 〈 P 〈 0.046）, while the factor potentially triggering hepatic cancer, transcrip- tion factor JUN-B, had a 20-fold higher expression. t

关键词： Metabotropic receptors Gene expression DNA oligonucleotides Quantitative real-time poly-merase chain reaction Chronic hepatitis

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