检索结果-南通市图书馆

Environmental enrichment combined with fasudil treatment inhibits neuronal death in the hippocampal CA1 region and ameliorates memory deficits

引用

Neural Regeneration Research 2021年第8期16卷 1460-1466页

作者： Gao-Jing Xu Qun Zhang Si-yue Li Yi-Tong Zhu Ke-Wei yu Chuan-Jie Wang Hong-yu xie Yi Wu Department of rehabilitation Medicine Huashan HospitalFudan UniversityShanghaiChina Department of Rehabilitation Medicine Jinshan Hospital of Fudan UniversityShanghaiChina

Currently, no specific treatment exists to promote recovery from cognitive impairment after a stroke. Dysfunction of the actin cytoskeleton correlates well with poststroke cognitive declines, and its reorganization requires proper regulation of Rho-associated kinase(ROCK) proteins. Fasudil downregulates ROCK activation and protects neurons against cytoskeleton collapse in the acute phase after stroke. An enriched environment can reduce poststroke cognitive impairment. However, the efficacy of environmental enrichment combined with fasudil treatment remains poorly understood. A photothrombotic stroke model was established in 6-week-old male C57 BL/6 mice. Twenty-four hours after modeling, these animals were intraperitoneally administered fasudil(10 mg/kg) once daily for 14 successive days and/or provided with environmental enrichment for 21 successive days. After exposure to environmental enrichment combined with fasudil treatment, the number of neurons in the hippocampal CA1 region increased significantly, the expression and proportion of p-cofilin in the hippocampus decreased, and the distribution of F-actin in the hippocampal CA1 region increased significantly. Furthermore, the performance of mouse stroke models in the tail suspension test and step-through passive avoidance test improved significantly. These findings suggest that environmental enrichment combined with fasudil treatment can ameliorate memory dysfunction through inhibition of the hippocampal ROCK/cofilin pathway, alteration of the dynamic distribution of F-actin, and inhibition of neuronal death in the hippocampal CA1 region. The efficacy of environmental enrichment combined with fasudil treatment was superior to that of fasudil treatment alone. This study was approved by the Animal Ethics Committee of Fudan University of China(approval No. 2019-Huashan Hospital JS-139) on February 20, 2019.

关键词： cognitive environment hippocampus injury neuroprotection pathway repair stroke

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Study on Vortex-Induced Motions of A New Type of Deep Draft Multi-Columns FDPSO

引用

China Ocean Engineering 2018年第1期32卷 1-13页

作者： GU Jia-yang xie yu-lin ZHAO yuan LI Wen-juan TAO Yan-wu HUANG Xiang-hong Marine Equipment and Technology Institute Jiangsu University of Science and Technology Zhenjiang 212003 China School of Naval Architecture and Marine Engineering Jiangsu University of Science and Technology Zhenjiang 212003China Marine Design & Research Institute of China Shanghai 200011 China

A numerical simulation and an experimental study on vortex-induced motion（VIM） of a new type of deep draft multi-columns floating drilling production, storage and offloading（FDPSO） are presented in this paper. The main dimension, the special variable cross-section column and the cabin arrangement of the octagonal pontoon are introduced based on the result. The numerical simulation is adapted to study the effects of current incidence angles and reduced velocities on this platform’s sway motion response. The 300 m water depth equivalent truncated mooring system is adopted for the model tests. The model tests are carried out to check the reliability of numerical simulation. The results consist of surge, sway and yaw motions, as well as motion trajectories. The maximum sway amplitudes for different types of offshore platform is also studied. The main results show that the peak frequencies of sway motion under different current incidence angles and reduced velocities vary around the natural frequency. The analysis result of flow field indicates that the change of distribution of vortex in vertical presents significant influences on the VIM of platform. The trend of sway amplitude ratio curve of this new type FDPSO differs from the other types of platform. Under 45° current incidence angle, the sway amplitude of this new type of FDPSO is much smaller than those of other types of offshore platform at 4.4 ≤ V;≤ 8.9. The typical ‘8’ shape trajectory does not appear in the platform’s motion trajectories.

关键词： FDPSO multi-columns vortex-induced motion(VIM) model tests variable cross-section column

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

功能性踝关节不稳男性下蹲跳过程中下肢的生物力学特征

引用

中国组织工程研究 2025年第3期29卷 478-485页

作者：王子龙孟昕张芷棋谢雨孟令越张秋霞孔翎宇苏州大学体育学院江苏省苏州市215000

背景:踝关节作为人体的末端承重关节,承受着机体自上而下的压力,这导致踝关节在运动中很容易发生损伤,诱发功能性踝关节不稳,对日常生活造成负面影响。研究功能性踝关节不稳患者在下蹲跳过程中的下肢生物力学表现,对实现科学训练、预防... 详细信息

背景:踝关节作为人体的末端承重关节,承受着机体自上而下的压力,这导致踝关节在运动中很容易发生损伤,诱发功能性踝关节不稳,对日常生活造成负面影响。研究功能性踝关节不稳患者在下蹲跳过程中的下肢生物力学表现,对实现科学训练、预防踝关节损伤以及伤后临床康复提供理论参考具有重要意义。目的:探究功能性踝关节不稳患者下蹲跳过程中的下肢运动学与动力学特征。方法:于2023年3-9月在苏州大学招募男性功能性踝关节不稳患者与健康人群各15名,年龄22-28岁,所有受试者均完成下蹲跳试验。采用Vicon红外高速运动捕捉系统和Kistler三维测力台对两组受试者在下蹲跳起跳阶段、离地瞬间时刻、初始着地时刻和垂直地面反作用力峰值时刻的下肢运动学和动力学指标进行同步采集及对比分析。结果与结论:(1)离地瞬间时刻,与健康对照组的匹配侧相比,功能性踝关节不稳组患侧显示出了更小的膝关节内旋力矩(P=0.020)、更小的踝关节内旋力矩(P=0.009)。(2)落地瞬间时刻,与健康对照组的匹配侧相比,功能性踝关节不稳组患侧显示出了更小的髋关节屈曲角度(P=0.039);功能性踝关节不稳组相较于健康对照组显示出了更小的髋关节外展角度(P=0.022)、更小的膝关节内翻角度(P=0.010)、更大的膝关节外旋角度(P=0.021)、更小的踝关节内翻角度(P=0.004)、更小的踝关节外旋角度(P=0.008)。(3)垂直地面反作用力峰值时刻,功能性踝关节不稳组相较于健康对照组显示出了更小的踝关节内翻角度(P=0.044);(4)结果说明,功能性踝关节不稳者在下蹲跳动作中相较于健康人群存在异常的下肢生物力学特征,主要表现为离地与落地瞬间时刻,下肢关节在矢状面和额状面的运动学和动力学指标变化。这些改变反映出功能性踝关节不稳人群在执行下蹲跳时采用了僵硬的起跳与着陆模式,倾向于将患侧踝关节负荷转移至下肢其他关节,并显示出健侧下肢的代偿现象。因此,检测和纠正异常生物力学特征应该成为功能性踝关节不稳者康复训练的一部分。

关键词：功能性踝关节不稳下蹲跳生物力学运动学参数动力学参数

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Protective effect of ginsenoside Rg1 on 661W cells exposed to oxygen-glucose deprivation/reperfusion via keap1/nrf2 pathway

引用

International Journal of Ophthalmology(English edition) 2023年第7期16卷 1026-1033页

作者： Ming Zhou Xin-Qi Ma Yi-yu xie Jia-Bei Zhou xie-Lan Kuang Huang-Xuan Shen Chong-De Long State Key Laboratory of Ophthalmology Zhongshan Ophthalmic CenterSun Yat-sen UniversityGuangdong Provincial Key Laboratory of Ophthalmology and Visual ScienceGuangzhou 510060China

AIM:To construct an in vitro model of oxygen-glucose deprivation/reperfusion(OGD/R)induced injury to the optic nerve and to study the oxidative damage mechanism of ischemia-reperfusion(I/R)injury in 661W cells and the protective effect of ginsenoside ***:The 661W cells were treated with different concentrations of Na2S2O4 to establish OGD/R model in ***,intracellular reactive oxygen species(ROS)levels and superoxide dismutase(SOD)levels were measured at different time points during the reperfusion injury *** injury model was pretreated with graded concentrations of ginsenoside ***-time polymerase chain reaction(PCR)was used to measure the expression levels of cytochrome C(cyt C)/B-cell lymphoma-2(Bcl2)/Bcl2 associated protein X(Bax),heme oxygenase-1(HO-1),caspase9,nuclear factor erythroid 2-related factor 2(nrf2),kelch-like ECH-associated protein 1(keap1)and other *** blot was used to detect the expression of nrf2,phosphorylated nrf2(pnrf2)and keap1 protein ***:Compared to the untreated group,the cell activity of 661W cells treated with Na2S2O4 for 6 and 8h decreased(P<0.01).Additionally,the ROS content increased and SOD levels decreased significantly(P<0.01).In contrast,treatment with ginsenoside Rg1 reversed the cell viability and SOD levels in comparison to the Na_(2)S_(2)O_(4)treated group(P<0.01).Moreover,Rg1 reduced the levels of caspase3,caspase9,and cyt C,while increasing the Bcl2/Bax *** differences were all statistically significant(P<0.05).Western blot analysis showed no significant difference in the protein expression levels of keap1 and nrf2 with Rg1 treatment,however,Rg1 significantly increased the ratio of pnrf2/nrf2 protein expression compared to the Na_(2)S_(2)O_(4)treated group(P<0.001).CONCLUSION:The OGD/R process is induced in 661W cells using Na_(2)S_(2)O_(4).Rg1 inhibits OGD/R-induced oxidative damage and alleviates the extent of apoptosis in 661W cells through the keap1/nrf2 pathway

关键词： oxygen-glucose deprivation/reoxygenation ginsenoside Rg1 oxidative stress phosphorylated nrf2

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Mechanistic evaluation of gastro-protective effects of KangFuXinYe on indomethacin-induced gastric damage in rats

引用

Chinese Journal of Natural Medicines 2020年第1期18卷 47-56页

作者： LI Qi-Juan WANG Zhan-Guo xie yu LIU Qiao HU Hui-Ling GAO Yong-Xiang College of Pharmacy Chengdu University of Traditional Chinese MedicineChengdu 611137China Metabonomics Synergy Innovation Laboratory School of Medicine and NursingChengdu UniversityChengdu 610106China College of Basic Medicine Chengdu University of Traditional Chinese MedicineChengdu 611137China

KangFuXinYe(KFX),the ethanol extract of the dried whole body of Periplaneta americana,is a well-known important Chinese medicine preparation that has been used to treat digestive diseases such as gastric ulcers for many years in ***,its therapeutic effect and mechanism are not yet well ***,the aim of this study was to investigate the gastroprotective effects of KangFuXinYe(KFX)in indomethacin-induced gastric *** were randomly divided into six groups as follows:control,treated with indomethacin(35 mg·kg^-1),different dosages of KFX(2.57,5.14 and 10.28 mL·kg^-1,respectively)plus indomethacin,and sucralfate(1.71 mL·kg^-1)plus *** treatment,rat serum,stomach and gastric homogenates were collected for biochemical tests and examination of histopathology *** serum was further used for metabolomics analysis to research possible *** results showed that KFX treatment alleviated indomethacin-induced histopathologic damage in rat gastric ***,its treatment significantly increased cyclooxygenase-1(COX-1),prostaglandin E2(PGE2)and epidermal growth factor(EGF)levels in rat serum and gastric ***,KFX decreased cyclooxygenase-2(COX-2)and interleukin-6(IL-6)*** metabolites were identified which intensities significantly changed in gastric damage rats,including 5-hydroxyindoleacetic acid,indoxylsulfuric acid,indolelactic acid,4-hydroxyindole,pantothenic acid,isobutyryl carnitine,3-methyl-2-oxovaleric acid,sphingosine 1-phosphate,and *** metabolic deviations came to closer to normal levels after KFX *** results indicate that KFX(10.28 mL·kg^-1)exerts protective effects on indomethacin-induced gastric damage by possible mechanisms of action(regulating tryptophan metabolism,protecting the mitochondria,and adjusting lipid metabolism,and reducing excessive indomethacin).

关键词： KangFuXinYe Indomethacin-induced gastric damage Inflammation Metabonomic analysis

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Recent advances of single-cell RNA sequencing technology in mesenchymal stem cell research

引用

World Journal of Stem Cells 2020年第6期12卷 438-447页

作者： Guan Zheng Zhong-yu xie Peng Wang Yan-Feng Wu Hui-Yong Shen Department of Orthopedics The Eighth Affiliated HospitalSun Yat-sen UniversityShenzhen 518033Guangdong ProvinceChina Center for Biotherapy Sun Yat-sen Memorial HospitalSun Yat-sen UniversityGuangzhou 510120Guangdong ProvinceChina

Mesenchymal stem cells(MSCs)are multipotent stromal cells with great potential for clinical ***,little is known about their cell heterogeneity at a single-cell resolution,which severely impedes the development of MSC *** this review,we focus on advances in the identification of novel surface markers and functional subpopulations of MSCs made by single-cell RNA sequencing and discuss their participation in the pathophysiology of stem cells and related *** challenges and future directions of single-cell RNA sequencing in MSCs are also addressed in this review.

关键词： Mesenchymal stem cells Single-cell RNA sequencing Pathophysiology Novel surface markers

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Synthesis and antibacterial activity of novel[1,2,4]triazolo[3,4-h][1,8]naphthyridine-7-carboxylic acid derivatives

引用

Chinese Chemical Letters 2015年第1期26卷 149-151页

作者： Liu-Zhou Gao yu-Suo xie Tao Li Wen-Long Huang Guo-Qiang Hu Institute of Chemical Biology.Henan University Centre of Drug Discovery China Pharmaceutical University

Novel tricyclic fluoroquinolones,[1,2,4]triazolo[3,4-h][1,8]naphthyridine-8-one-7-carboxylic acid derivatives 4a-4h bearing carrying a functional Mannich-base moiety at the C-8 position,were synthesized and evaluated for their antimicrobial *** results showed that some compounds with a piperazine side chain exhibited comparable or better antibacterial activity than comparator ***,the targeted compounds also displayed a broad spectrum of activity against resistant strains including both Gram-negative and Gram-positive *** particular,compound 4h showed an MIC of 0.25 μg/mL in antibacterial assay against multiple drug-resistant Escherichia coli,which represents an about 30-fold increase of potency compared to ***,their excellent antibacterial activity against resistant strains suggests that triazole-fused fluoroquinolones warrant further optimization as novel anti-infective chemotherapies.

关键词： Triazole Tricyclic fluoroquinolone Synthesis Antibacterial activity

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Precipitation and heterogeneous strengthened CoCrNi-based medium entropy alloy with excellent strength-ductility combination from room to cryogenic temperatures

引用

Science China(Technological Sciences) 2022年第8期65卷 1780-1797页

作者： xie yu ZHAO PengCheng TONG YongGang TAN JianPing SUN BinHan CUI Yan WANG RunZi ZHANG XianCheng TU ShanTung Key Laboratory of Pressure Systems and Safety Ministry of EducationSchool of Mechanical and Power EngineeringEast China University of Science and TechnologyShanghai 200237China College of Automobile and Mechanical Engineering Changsha University of Science and TechnologyChangsha 410114China Key Laboratory for Advanced Materials and Feringa Nobel Prize Scientist Joint Research Center Institute of Fine ChemicalsSchool of Chemistry&Molecular EngineeringEast China University of Science and TechnologyShanghai 200237China

The improved understandings of the mechanical properties as well as deformation mechanisms at cryogenic temperatures are the prerequisite for realizing the application of any new engineering materials to cryogenic ***,a(CoCrNi)_(94)Al_(3)Ti_(3) medium entropy alloy(MEA)with nanoscale L12 coherent precipitates and heterogeneous grain structures was prepared by codoping Al and Ti elements with subsequent cold rolling and heat treatment *** mechanical properties were evaluated at the temperature range of 293–113 *** ultimate strength of the MEA increases almost linearly from 1326 to 1695 MPa as the temperature decreases from 293 to 113 K,while the total elongation remains approximately constant of~35%.The underlying deformation and strengthening mechanisms were investigated using various characterization *** to the effect of co-doped Al/Ti on channel width of the matrix and the increasing critical twinning stress induced by heterogeneous ultrafine grain size,the formation of deformation twins is inhibited at all ***,only a slight increase of the deformation twins and stacking faults in the deformed specimens with a decreasing temperature,which leads to the relative temperature-independence of the *** dislocation cutting mechanism of L1_(2) coherent precipitates and the heterodeformation induced(HDI)hardening both significantly contribute to the strain hardening so that an excellent combination of strength and ductility is ***,the evolution of lattice friction stress with deformation temperature is determined by quantitative analysis,indicating an approximately linear relationship between the lattice friction and *** present work provides new insights into the strategy of achieving outstanding strength-ductility synergy of the MEA under the wide temperature range by coupling heterogeneous ultrafine-grained structure and coherent precipitation strategy.

关键词： medium entropy alloy cryogenic temperature heterogeneous structure precipitation strengthening twinning

来源：

维普期刊数据库

同方期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

ZmGns,a maize class I β-1,3-glucanase,is induced by biotic stresses and possesses strong antimicrobial activity

引用

Journal of Integrative Plant Biology 2015年第3期57卷 271-283页

作者： yu-Rong xie Yenjit Raruang Zhi-yuan Chen Robert L.Brown Thomas E.Cleveland Department of Plant Pathology and Crop Physiology Louisiana State University Agricultural Center Biotechnology Research Institute the Chinese Academy of Agricultural Sciences Southern Regional Research Center USDA-ARS

Plant b-1,3-glucanases are members of the pathogenesis-related protein 2（PR-2） family,which is one of the 17 PR protein families and plays important roles in biotic and abiotic stress *** of the differentially expressed proteins（spot 842） identified in a recent proteomic comparison between five pairs of closely related maize（Zea mays L.） lines differing in aflatoxin resistance was further investigated in the present ***,the corresponding cDNA was cloned from maize and designated as *** encodes a protein of338 amino acids containing a potential signal *** expression of Zm Gns was detectible in all tissues studied with the highest level in *** was significantly induced by biotic stresses including three bacteria and the fungus Aspergillus *** was also induced by most abiotic stresses tested and growth hormones including salicylic *** vivo,ZmGns showed a significant inhibitory activity against thebacterial pathogen Pseudomonas syringae *** DC3000 and fungal pathogen Botrytis cinerea when it overexpressed in *** high level of expression in the silk tissue and its induced expression by phytohormone treatment,as well as by bacterial and fungal infections,suggest it plays a complex role in maize growth,development,and defense.

关键词： β-1 3-Glucanase biological activity expression profiles Zea mays ZmGns

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

ZnO nanorod arrays:Dependence of morphology upon ammonia

引用

Frontiers of Chemistry in China 2011年第4期6卷 355-357页

作者： yu xie Dan LIU Ming GAO Ru TAN Yan WANG Dongqi yu Liaoning Normal University Dalian 116024China Dalian National Laboratory for Clean Energy Dalian 116023China

Well-aligned ZnO nanorod arrays were prepared on FTO substrate by hydrothermal method at low temperature for 5 *** effect of ammonia on the length of ZnO nanorod was studied in *** resulting materials were extensively characterized by scanning electron microscopy(SEM),X-ray diffraction(XRD)and UV-visible absorption spectra(scatter mode).With the increase of ammonia,the length of ZnO nanorod increases.

关键词： ammonia ZnO nanorods length

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：