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Effects of Aronia polyphenols on the physico-chemical properties of whey,soy,and pea protein isolate dispersions

Food Production, Processing and Nutrition 2021年第1期3卷 391-405页

作者： Mackenzie M.hansen Richard W.hartel yrjö h.roos Food Technology School of Food and Nutritional SciencesUniversity College CorkCorkIreland Department of Food Science College of Agricultural and Life SciencesUniversity of WisconsinOffice A11Babcock Hall1605 Linden DriveMadisonWI 53706USA

Bioactive compounds including polyphenols(PP)have been observed to naturally form non-covalent complexation interactions with proteins under mild ph and temperature conditions,affecting protein structures and ***,addition of Aronia berry PP to liquid dispersions containing whey protein isolate(WPI)and sucrose was found to alter characteristics including viscosity,surface tension,and particle sizes,with changes being attributed to protein-PP *** this study we aimed to investigate whether Aronia PP would interact with soy and pea protein isolates(SPI and PPI,respectively)to a similar extent as with WPI in liquid protein-sucrose-PP *** hypothesized that formulations containing PPI(comprised of larger proteins)and hydrolyzed SPI(containing more carboxyl groups)may exhibit increased viscosities and decreased aggregate sizes due to enhanced protein-PP *** liquid dispersions of varied ratios of protein to sucrose contents,containing different protein isolates(WPI,SPI,and PPI),and varied Aronia PP concentrations were formulated,and physical properties were evaluated to elucidate the effects of PP *** addition altered physical characteristics differently depending on the protein isolate used,with changes attributed to protein-PP *** and PPI appeared to have higher propensities for PP interactions and exhibited more extensive shifts in physical properties than WPI *** findings may be useful for practical applications such as formulating products containing fruit and proteins to obtain desirable sensory attributes.

关键词： Whey protein Soy protein Pea protein Polyphenols Concentrated dispersions Physico-chemical properties

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Bioactives and extracts affect the physico-chemical properties of concentrated whey protein isolate dispersions

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Food Production, Processing and Nutrition 2022年第1期4卷 25-40页

作者： Mackenzie M.hansen Richard W.hartel yrjöh.roos Food Technology School of Food and Nutritional SciencesUniversity College CorkCorkIreland Department of Food Science College of Agricultural and Life SciencesUniversity of WisconsinOffice A11Babcock Hall1605 Linden DriveMadisonWI53706USA

Non-covalent complexation interactions are known to occur between bioactive compounds and proteins. While formulating with these components can have positive outcomes such as stabilization of colors and actives, it can also result in changes to the structures and physical properties of proteins, affecting product functionality and sensory attributes. Previous experiments reported measurable changes in the physico-chemical properties of whey protein isolate (WPI) dispersions upon formulation with Aronia berry extract, ascribing changes to protein-polyphenol (PP) interactions in the systems. Pure gallotannin, beet extract, and cranberry extract, providing a diverse variety structures and sizes, were selected for further experimentation and comparison with the effects of Aronia extract. Concentrated dispersions with varying WPI:sucrose ratios, formulated with several bioactives contents from multiple different sources were analyzed to identify the effects of different bioactives on physico-chemical properties of dispersions. Dispersions formulated with cranberry extract demonstrated the largest increases in surface tensions, viscosities, and particle sizes, while those formulated with beet extract were the least affected by the presence of bioactives, suggesting that different bioactives and extracts had varying propensities for complexation interactions with WPI, despite their relatively low levels of addition (0, 0.5, and 1%).

关键词： Whey protein Concentrated dispersions Polyphenols Extracts Physico-chemical properties

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Superconvergence of a Galerkin FEM for higher-Order Elements in Convection-Diffusion Problems

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Numerical Mathematics(Theory,Methods and Applications) 2014年第3期7卷 356-373页

作者： Sebastian Franz h.-G.roos Institut für Numerische Mathematik Technische Universit¨at Dresden01062 DresdenGermany

In this paper we present a first supercloseness analysis for higher-order Galerkin FEM applied to a singularly perturbed convection-diffusion *** a solution decomposition and a special representation of our finite element space,we are able to prove a supercloseness property of p+1/4 in the energy norm where the polynomial order p≥3 is odd.

关键词： Singular perturbation layer-adapted meshes superconvergence postprocessing

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Building Expertise on FAIR Through Evolving Bring Your Own Data(BYOD) Workshops: Describing the Data, Software, and Management-focused Approaches and Their Evolution

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Data Intelligence 2024年第2期6卷 429-456页

作者： César h.Bernabé Lieze Thielemans Rajaram Kaliyaperumal Claudio Carta Shuxin Zhang Celia W.G.van Gelder Nirupama Benis Luiz Olavo Bonino da Silva Santos Ronald Cornet Bruna dos Santos Vieira Nawel Lalout Ines henriques Alberto Camara Ballesteros Kees Burger Martijn G.Kersloot Friederike Ehrhart Esther van Enckevort Chris T.Evelo Alasdair J.G.Gray Marc hanauer Kristina hettne Joep de Ligt Arnaldo Pereira Nuria Queralt-Rosinach Erik Schultes Domenica Taruscio Andra Waagmeester Mark D.Wilkinson Egon L.Willighagen Mascha Jansen Barend Mons Marco roos Annika Jacobsen Biosemantics Group Department of Human GeneticsLeiden University Medical CentreLeidenThe Netherlands NIhR Academic Fellow in General Practice University College London and The Royal Free NHS Foundation Trust National Centre for Rare Diseases Istituto Superiore di SanitaRomeItaly Department of Medical Informatics Amsterdam UMC location University of AmsterdamMeibergdreef 9AmsterdamThe Netherlands Amsterdam Public health Methodology&Digital HealthAmsterdamThe Netherlands health-Rl UtrechtThe Netherlands Semantics Services and Cybersecurity groupUniversity of TwenteEnschedeThe Netherlands Department of Medical Imaging Radboud Institute for Health SciencesRadboud University Medical CenterNijmegenThe Netherlands Department of Medical BioSciences Radboud University Medical CenterNijmegenThe Netherlands The Duchenne Parent Project VeneendaalThe Netherlands Departamento de Biotecnologia-Biologia Vegetal Escuela Tecnica Superior de Ingenieria AgronomicaAlimentaria y de BiosistemasCentro de Biotecnologia y Genomica de PlantasUniversidad Politecnica de Madrid(UPM)-Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria-CSIC(INIA-CSIC).Campus MontegancedoPozuelo de Alarcon(Madrid)Spain Castor AmsterdamThe Netherlands Department of Bioinformatics-BiGCaT Maastricht UniversityMaastrichtThe Netherlands University Medical Center Groningen University of GroningenGroningenThe Netherlands Maastricht Centre for Systems Biology(MaCSBio) Maastricht UniversityMaastrichtThe Netherlands Department of Computer Science Heriot-Watt UniversityEdinburghUK INSERM US14-OrphanetPlateforme Maladies RaresParisFrance Centre for Digital Scholarship Leiden University LibrariesThe Netherlands Center for Molecular Medicine Cancer Genomics NetherlandsDepartment of GeneticsUniversity Medical Center UtrechtUniversity UtrechtThe Netherlands University of Aveiro DETI/IEETAAveiroPortugal GO FAIR Foundation LeidenThe Netherlands The Leiden Academic Center for Drug Research LeidenThe Netherlands Micel

Since 2014,"Bring Your Own Data"workshops(BYODs)have been organised to inform people about the process and benefits of making resources Findable,Accessible,Interoperable,and Reusable(FAIR,and the FAIRification process).The BYOD workshops'content and format differ depending on their goal,context,and the background and needs of ***-focused BYODs educate domain experts on how to make their data FAIR to find new answers to research ***-focused BYODs promote the benefits of making data FAIR and instruct project managers and policy-makers on the characteristics of FAIRification ***-focused BYODs gather software developers and experts on FAIR to implement or improve software resources that are used to support ***,these BYODs intend to foster collaboration between different types of stakeholders involved in data management,curation,and reuse(*** experts,trainers,developers,data owners,data analysts,FAIR experts).The BYODs also serve as an opportunity to learn what kind of support for FAIRification is needed from different communities and to develop teaching materials based on practical examples and *** this paper,we detail the three different structures of the BYODs and describe examples of early BYODs related to plant breeding data,and rare disease registries and biobanks,which have shaped the structure of the *** discuss the latest insights into making BYODs more productive by leveraging our almost ten years of training experience in these workshops,including successes and encountered ***,we examine how the participants'feedback has motivated the research on FAIR,including the development of workflows and software.

关键词： FAIR FAIRification FAIR expertise Bring Your Own Data Workshop BYOD

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UNIFORM SUPERCONVERGENCE OF A FINITE ELEMENT METhOD WITh EDGE STABILIZATION FOR CONVECTION-DIFFUSION PROBLEMS

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Journal of Computational Mathematics 2010年第1期28卷 32-44页

作者： Sebastian Franz Torsten Linβ hans-Grg roos Sebastian Schiller Department of Mathematics and Statistics University of Limerick Institut für Numerische Mathematik Technische Universitt Dresden

In the present paper the edge stabilization technique is applied to a convection-diffusion problem with exponential boundary layers on the unit square, using a Shishkin mesh with bilinear finite elements in the layer regions and linear elements on the coarse part of the mesh. An error bound is proved for ‖πu-u^h‖Е, where πu is some interpolant of the solution u and uh the discrete solution. This supercloseness result implies an optimal error estimate with respect to the L2 norm and opens the door to the application of postprocessing for improving the discrete solution.

关键词： Convection-diffusion problems Edge stabilization FEM Uniform convergence Shishkin mesh.

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Characteristics and Affinity of Tropical Flora in Southern China

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Forestry Studies in China 2002年第1期4卷 18-24页

作者：朱华 M.C.roos 中国科学院西双版纳热带植物园昆明分部昆明650223 莱顿大学荷兰国家标本馆

The tropical flora in southern China is represented mainly by three regional flora, i.e. southern Yunnan (southwestern China), southwestern Guangxi (southwestern China) and hainan Island (southern China). The floristic composition of each is concisely enumerated. Their geographical elements at generic level are analyzed. Furthermore, a comparison of floristic similarities between southern Yunnan and hainan Island and Malay Peninsula and Brunei of western Malesia is made, based on existing regional flora treatments. The following is concluded: (1) The flora of southern China consists mainly of tropical floristic elements which contribute about 60% at the family level and more than 80 % at the generic level of its total flora. The dominant distribution type of the flora of southern China at the generic level is the tropical Asian distribution pattern. This reveals that the flora of southern China is of tropical nature with a strong tropical Asian affinity; (2) Most of the dominant families from the flora of southern China are also dominant in the Malesian flora. The floristic similarities between the regional flora of southern China and the regional flora of western Malesia are over 70% at the family level and more than 50% at the generic level. This suggests that the tropical flora of southern China belongs to the Malesian floristic region; (3) Situated at the northern margin of tropical Asia, on the other hand it is also obvious that the flora of southern China comprises less strictly tropical elements compared to the Malesian flora, and is consequently only a marginal type of the latter; (4) The close affinity of the flora of southern China to the Malesian flora can be explained by the geological history of southeast Asia.

关键词： southern China flora floristic composition affinity

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Making FAIR Easy with FAIR Tools:From Creolization to Convergence

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Data Intelligence 2020年第1期2卷 87-95,305页

作者： Mark Thompson Kees Burger Rajaram Kaliyaperumal Marco roos Luiz Olavo Bonino da Silva Santos Leiden University Medical Center Leiden2333 ZAThe Netherlands GO FAIR international Support&Coordination Office(GFISCO) LeidenThe Netherlands

Since their publication in 2016 we have seen a rapid adoption of the FAIR principles in many scientific disciplines where the inherent value of research data and,therefore,the importance of good data management and data stewardship,is *** has led to many communities asking“What is FAIR?”and“how FAIR are we currently?”,questions which were addressed respectively by a publication revisiting the principles and the emergence of FAIR ***,early adopters of the FAIR principles have already run into the next question:“how can we become(more)FAIR?”This question is more difficult to answer,as the principles do not prescribe any specific standard or ***,there does not yet exist a mature ecosystem of tools,platforms and standards to support human and machine agents to manage,produce,publish and consume FAIR data in a user-friendly and efficient(i.e.,“easy”)*** this paper we will show,however,that there are already many emerging examples of FAIR tools under *** paper puts forward the position that we are likely already in a creolization phase where FAIR tools and technologies are merging and combining,before converging in a subsequent phase to solutions that make FAIR feasible in daily practice.

关键词： FAIR data FAIR in practice FAIR tools FAIR application support creolization and convergence

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Effect of using ascorbic acid and cysteamine supplementation onin-vitrodevelopment of buffalo embryos

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Asian pacific Journal of Reproduction 2017年第2期6卷 85-88页

作者： Al-shimaa Al-h.h.El-Naby Karima Gh.M.Mahmoud Gamal A.M.Sosa Mahmoud E.A.Abouel-roos Youssef F.Ahmed Department of Theriogenology Faculty of Veterinary MedicineBenha UniversityEgypt Department of Animal Reproduction and A.I. National Research CenterDokkiGizaEgypt

Objective:To improvein vitro embryo production in buffalo by supplementation of L-ascorbic acid during maturation and development (experiment 1) and combination with another antioxidant as cysteamine (experiment 2).Methods:Two experiments were performed, the first one aimed to evaluate the different concentrations (0, 25, 50, 100 μM) of L-ascorbic acid on embryo developmental rate of buffalo oocytes. The L-ascorbic acid was added to the maturation and culture media. In the second experiment, oocytes were cultured in media with two type of antioxidant (ascorbic acid + cysteamine) or ascorbic acid ***:There was a significant increase in cleavage rate at 25, 50 μM than 100 μM and control group. But, the blastocyst rate was higher at 50 μM ascorbic acid than other concentrations (0, 25, 100 μM). Supplementation of ascorbic acid and cysteamine to maturation and cultured media improved embryo development than ascorbic acid ***: Using of 50 μM L-ascorbic acid duringin vitro maturation and development improve the developmental competence of buffalo oocytes, this effect was increase with the presence of cysteamine.

关键词： Buffalo Oocytes L-ascorbic acid Cysteamine Embryo development

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A Generic Workflow for the Data FAIRification Process

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Data Intelligence 2020年第1期2卷 56-65页

作者： Annika Jacobsen Rajaram Kaliyaperumal Luiz Olavo Bonino da Silva Santos Barend Mons Erik Schultes Marco roos Mark Thompson Leiden University Medical Center Leiden2333 ZAThe Netherlands GO FAIR International Support&Coordination Office(GFISCO) LeidenThe Netherlands

The FAIR guiding principles aim to enhance the Findability,Accessibility,Interoperability and Reusability of digital resources such as data,for both humans and *** process of making data FAIR(“FAIRification”)can be described in multiple *** this paper,we describe a generic step-by-step FAIRification workflow to be performed in a multidisciplinary team guided by FAIR data *** FAIRification workflow should be applicable to any type of data and has been developed and used for“Bring Your Own Data”(BYOD)workshops,as well as for the FAIRification of e.g.,rare diseases *** steps are:1)identify the FAIRification objective,2)analyze data,3)analyze metadata,4)define semantic model for data(4a)and metadata(4b),5)make data(5a)and metadata(5b)linkable,6)host FAIR data,and 7)assess FAIR *** each step we describe how the data are processed,what expertise is required,which procedures and tools can be used,and which FAIR principles they relate to.

关键词： FAIR data FAIRification workflow FAIR data stewardship hands-on FAIRification FAIR dissemination

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单个腺病毒颗粒的原子力显微镜液相成像及样品制备方法

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病毒学报 2015年第6期31卷 601-606页

作者：梁艳李晨 Mariska G M van Rosmalen Gijs J L Wuite Wouter h.roos 中国水产科学研究院黄海水产研究所青岛266071 阿姆斯特丹自由大学

原子力显微镜(Atomic force microscopy,AFM)作为一种纳米级高分辨率的探针扫描显微镜,在病毒形态学研究等领域有广泛的应用。为更好地利用AFM在接近病毒生理环境(液相)中观测单个病毒颗粒,选择合适的基底吸附病毒样品是重要的前提保障... 详细信息

原子力显微镜(Atomic force microscopy,AFM)作为一种纳米级高分辨率的探针扫描显微镜,在病毒形态学研究等领域有广泛的应用。为更好地利用AFM在接近病毒生理环境(液相)中观测单个病毒颗粒,选择合适的基底吸附病毒样品是重要的前提保障。本文介绍了一种病毒样品制备及AFM液相成像方法。首先制备疏水化处理的玻璃盖玻片作为吸附基底,再将观察样品腺病毒重组载体颗粒(Adenovirus type 5F35,Ad5F35)吸附于基底上,建立在液相下单个病毒颗粒的原子力显微镜成像方法。通过该方法获取了单个腺病毒颗粒在液相下的高分辨率纳米级高度图(height image),且病毒颗粒保持了约90nm的正确测量高度。实验结果表明疏水化处理的玻璃基底具有良好的吸附力,有助于病毒颗粒牢固地吸附在基底上,且不会引起较大的形变。本研究为AFM观测病毒等生物样品提供了一种较为合适的吸附基底和在液相中的AFM成像方法,对后期开展病毒形态结构、物理属性及其与宿主细胞受体相互作用的研究打下基础。

关键词：原子力显微镜液相成像样品制备腺病毒颗粒

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