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Deciphering Starch Quality of Rice Kernels Using Metabolite Profiling and Pedigree Network Analysis

Molecular Plant 2012年第2期5卷 442-451页

作者： Miyako Kusano Atsushi Fukushima Naoko Fujita yozo okazaki Makoto Kobayashi Naoko Fujita Oitome Kaworu Ebana Kazuki Saito RIKEN Plant Science Center Tsurumi Yokohama 230-0045 Japan KIHARA Institute for Biological Research Yokohama City University Totsuka Yokohama 244-0813 Japan Department of Biological Production Akita Prefectural University Akita 010-0195 Japan National Institute of Agrobiological Sciences 2-1-2 Kannondai Tsukuba Ibaraki 305-8602 Japan Graduate School of Pharmaceutical Sciences Chiba University Chiba 260-8675 Japan

The physiological properties of rice grains are immediately obvious to consumers. High-coverage metabolomic characterization of the rice diversity research set predicted a negative correlation between fatty acid and lipid levels and amylose/total starch ratio （amylose ratio）, but the reason for this is unclear. To obtain new insight into the relationships among the visual phenotypes of rice kernels, starch granule structures, amylose ratios, and metabolite changes, we investigated the metabolite changes of five Japonica cultivars with various amylose ratios and two knockout mutants （e 1, a Starch synthase Ilia （SSIIla）-deficient mutant and the SSIIla/starch branching enzyme （BE） double-knockout mutant 4019） by using mass spectrometry-based metabolomics techniques. Scanning electron microscopy clearly showed that the two mutants had unusual starch granule structures. The metabolomic compositions of two cultivars with high amylose ratios （Hoshiyutaka and Yumetoiro） exhibited similar patterns, while that of the double-knockout mutant, which has an extremely high amylose ratio, differed. Rice pedigree network analysis of the cultivars and the mutants provided insight into the association between metabolic-trait properties and their underlying genetic basis in rice breeding in Japan. Multidimensional scaling analysis revealed that the Hoshiyutaka and Yumetoiro cultivars were Indica-like, yet they are classified as Japonica subpopulations. Exploring metabolomic traits is a powerful way to follow rice genetic traces and breeding history.

关键词： starch quality traits amylose ratio metabolite profiling pedigree networks rice.

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Effects of Pressure, Solvent, and Substituent on the Thermal Isomerization of 2-Oxo- spiro[diazirine-3,3'-indoline]

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Chemical Research in Chinese Universities 2001年第1期17卷 95-101页

作者： Yoshimi Sueishi Genta okazaki Department of Chemistry Faculty of ScienceOkayama UniversityTsushima Naka 3-1-1Okayama 700-8530Japan

The kinetic effects of pressure, solvent, and substituent on the thermal isomerization from 2 oxospiro[diazirine 3,3 indoline] to 3 diazo 2 oxo indoline have been examined. The rate constants of the thermal isomerization increased with increasing external pressure and were related to the σ + P substituent constant of Hammett correlation. The results suggest that the thermal isomerization proceeds via a dipolar transition state involving heterolytic bond fission. However, the kinetic solvent effects on the thermal isomerization were not observed. The special solvent effects were discussed on the basis of the linear free energy relationship.

关键词： Kinetic study Pressure effect Substituent effect Thermal isomerization Diazirine

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Electron scattering by Friedel oscillations in carbon nanotubes

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Nano Research 2022年第2期15卷 889-897页

作者： Takumi Inaba Takahiro Morimoto Satoshi Yamazaki Toshiya okazaki CNT-Application Research Center National Institute of Advanced Industrial Science and TechnologyTsukuba 305-8565Japan Research Association of High-Throughput Design and Development for Advanced Functional Materials Tsukuba 305-8565Japan

Multi-walled carbon nanotube networks were confirmed to exhibit a linear decrease in resistivity with increasing temperature from 100 to above 400 *** linearity was explained using a defect scattering model that involved Friedel oscillations(that is,electron-electron interactions).The applicability of this model,which was originally proposed for graphene,to carbon nanotubes was assessed based on a comparison of various experimental *** in the slopes of the resistivity-temperature plots following the introduction of defects,as well as an effect of charge concentration on the slope were key predictions of this *** results obtained from few-walled carbon nanotube networks are also *** the literature,linear resistivity-temperature plots were obtained from other graphene derivatives,indicating that the linearity originates from the hexagonal symmetry of these *** present work also indicated a relationship between the appearance of linearity and negative magnetoresistance above 100 *** on a mechanism incorporating scattering in association with Friedel oscillations and conventional electron conduction models,the universality of resistivity-temperature plots obtained from carbon nanotube networks is introduced.

关键词： carbon nanotube electron transport electron-electron interaction Friedel oscillations magnetoresistance

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MICROMECHANICS OF THE DAMAGE-INDUCED CELLULAR MICROSTRUCTURE IN SINGLE CRYSTAL Ni-BASED SUPERALLOYS

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Acta Metallurgica Sinica(English Letters) 2004年第4期17卷 361-368页

作者： M.Sakaguchi M.okazaki Department of Mechanical Engineering Nagaoka University of Technology Tomioka Nagaoka 1603-1JapanDepartment of Mechanical Engineering Nagaoka University of Technology Tomioka Nagaoka 1603-1Japan

An analytical method to investigate the morphological evolution of the cellular mi-crostructure is explored and proposed. The method is essentially based on the Es-helby 's micromechanics theory, and it is extended so as to be applied for a material system containing inclusions with high volume fraction, by employing the average stress field approximation by Mori and Tanaka. The proposed method enables us to discuss a stable shape of precipitate in the material system, which must be influenced by many factors: e.g., volume fraction of precipitate; Young's modulus ratio and lattice misfit between matrix and precipitate; external stress field in multiaxial state; and heterogeneity of plastic strain between matrix and precipitate. A series of numerical calculations were summarized on stable shape maps. The application of the method to predict the γ' rafting in superalloys during creep showed that the heterogeneity of plastic strain between matrix and precipitates may play a significant role in the shape stability of the precipitate. Furthermore, it was shown that the method was successfully applied to estimate the morphology of the cellular microstructure formed in CMSX-4 single crystal Ni-based superalloy.

关键词： single crystal Ni-based superalloy cellular microstructure mi-cromechanics average field approximation stable shape of precipitate heterogeneity of plastic strain rafting

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Improvement of aerodynamic stability of suspension bridges with H-shaped simplified stiffening girder

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Frontiers of Structural and Civil Engineering 2016年第1期10卷 93-102页

作者： Hiroshi KATSUCHI Hitoshi YAMADA Mayuko NISHIO Yoko okazaki Department of Civil Engineering Yokohama National University Yokohama 240-8501 Japan

Wind-tunnel study on the improvement of aerodynamic stability of simplified suspension-bridge girder structures was conducted with using a 1/40-scaled section model. Objective of the study is the development of an economically superior suspension bridge with 500-1,000 m center span length. The wind-tunnel test showed that an edge-girder type cross section exhibited large amplitude torsional vortex-induced vibration as well as torsional flutter at a low wind speed. Accordingly, aerodynamic countermeasures of open grating deck and triangular fating, and structural countermeasures of center stay, diagonal bracing and mass increase were tried to improve the aerodynamic stability. Finally, feasibility of the best combination to a full-scale bridge was examined by structural analysis.

关键词： suspension bridge aerodynamics simplified girder wind-tunnel test

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The critical stage for inducing oviparity and embryonic diapause in parthenogenetic Artemia(Crustacea: Anostraca): an experimental study

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Journal of Oceanology and Limnology 2019年第5期37卷 1669-1677页

作者： WANG Zhichao Alireza ASEM Robert K. okazaki SUN Shichun Institute of Evolution & Marine Biodiversity Ocean University of ChinaQingdao 266003China College of Life Science Tarim UniversityAlar 843300China School of Life Science and Ecology Hainan Tropical Ocean UniversitySanya 572022China Department of Zoology Weber State University1415 Edvalson StreetDept 2505OgdenUT 84408-2505USA

The brine shrimp Artemia exhibits two reproductive modes: 1) oviparity, producing diapause embryos;and 2) ovoviviparity, producing free-swimming nauplii. Previous studies have suggested the existence of a critical stage that determines the reproductive mode. Physicochemical factors, such as photoperiod, temperature, and salinity, have been suggested to irreversibly aff ect the reproductive mode of oocytes during this critical stage. In this study, experiments were carried out using a photoperiod and temperature-sensitive parthenogenetic Artemia clone where maternal Artemia were shifted bidirectionally between ovoviviparity (18 h L:6 h D, 27℃) and oviparity (6 h L:18 h D, 19℃) culture conditions. In the main experiment ( Artemia shifted at six diff erent stages including the post-larva Ⅱ to adult Ⅱ), the reproductive mode of fi rst brood was converted when shifting was performed on post-larva Ⅱ and Ⅲ but was not converted when females were shifted after post-larva Ⅲ. A supplementary experiment further revealed that the reproductive mode of fi rst brood could be altered when shifting females at an "early phase of postlarva Ⅳ", characterized by a developing ovisac reaching the middle of the third abdominal segment, ventral spines, and some oocytes growing larger than the others. In both experiments, reproductive modes of the second brood were signifi cantly aff ected when the shifting was performed on post-larva Ⅳ. These results suggest that the critical stage for inducing oviparity and embryonic diapause is at the previtellogenic stage of oocytes, or at maternal "early phase of post-larva Ⅳ" for the first-brood off spring. During this stage, diff erential gene expression patterns of the two destined oocytes may be triggered by the token stimuli signals received by the oocytes.

关键词： Artemia reproduction mode environmental factors diapause critical stage Anostraca

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Photostabilizing effects of singlet oxygen quenchers on long chain cyanine dyes

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Science China Chemistry 1995年第11期38卷 1298-1305页

作者：曾万学陈萍郑德水 Tsuneki okazaki Masaaki Hayami Institute of Photographic Chemistry Chinese Academy of SciencesBeijing 100101China Nippon Kankoh-Shikiso Kenkyusho Okayama 701Japan

The total rate constants of the reaction of six antioxidants with singlet oxygen were measured by fluorospectroscopy,and the effects of these antioxidants on the photostability of the dyes were also investigated *** results reveal that the six antioxidants are the effective quenchers of singlet oxygen (~1O_2)and can increase the photostability of the *** power of metal chelates to quench ~1O_2 is the strongest,and the chelates show the most effects on the photostability of the *** photostability of the dyes has something to do with the relative amount of the antioxidant used.

关键词： long chain cyanine dye singlet oxygen quencher photooxidation

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PRODUCTION OF HIGH QUALITYLUBRICATING BASE OIL

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石油学报（石油加工） 2001年第1期17卷 77-80页

作者： Hajime okazaki Michiaki ADACHI Jin-ichi IGARASHI Central Technical Research Laboratory Nippon Mitsubishi Oil Corporation8 Chidori-cho Naka-ku YokohamaKanagawa 231-0815

High VI lubricating oil is produced in hydrocracker through hydrocracking (HDC) and hydroisome-rization reactions. In order to effectively produce high VI component, such as iso-pafaffins and monocyclic naphtenes, it is important to load suitable HDC catalysts and operate them in the appropriate reaction conditions.　　Nippon Mitsubishi Oil Corporation (NMOC) and its affiliate company, Nippon Mitsubishi Petroleum Refining Company (NMPRC) reported their original HDC catalysts four years ago in this Japan-China joint se-minar in Beijing［1］. NMOC and NMPRC operate their hydrocracker both in fuel oil production mode and in lubricating oil production mode. In lubricating oil production mode, high VI lubricating oil called VHDC are produced.　　In this paper, at first, the advantages of high VI lubricating oil are described. And then it is announced that NMOC and NMPRC have developed a new generation of HDC catalyst with higher cracking activity, higher middle distillate selectivity and longer life than the other commercial HDC catalysts. In addition to those properties, the catalyst is able to yield high VI lubricating oil as well.

关键词：润滑油质量管理原油加工加氢裂化 HDC

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Long-term outcomes of autoimmune pancreatitis

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World Journal of Gastroenterology 2016年第34期22卷 7760-7766页

作者： Tsukasa Ikeura Hideaki Miyoshi Masaaki Shimatani Kazushige Uchida Makoto Takaoka Kazuichi okazaki Department of Gastroenterology and Hepatology Kansai Medical University

Autoimmune pancreatitis(AIP) has been considered a favorable-prognosis disease; however,currently,there is limited information on natural course of AIP during long-term follow-up. Recently published studies regarding the long-term outcomes of AIP has demonstrated the developments of pancreatic stone formation,exocrine insufficiency,and endocrine insufficiency are observed in 5%-41%,34%-82%,and 38%-57% of patients having the disease. Furthermore,the incidence rate of developing pancreatic cancer ranges from 0% to 4.8% during the long-term followup. The event of death from AIP-related complications other than accompanying cancer is likely to be rare. During follow-up of AIP patients,careful surveillance for not only relapse of the disease but also development of complications at regular intervals is needed.

关键词： Autoimmune pancreatitis Outcome Cancer Prognosis

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Long-term survival of the Sister Mary Joseph nodule originating from breast cancer:A case report

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World Journal of Clinical Cases 2023年第34期11卷 8205-8211页

作者： Koji Kanayama Maki Tanioka Yoshitsugu Hattori Takuya Iida Mutsumi okazaki Department of Plastic and Reconstructive Surgery The University of TokyoTokyo 113-8655Japan Department of Medical Oncology National Cancer Center HospitalTokyo 104-0045Japan

BACKGROUND A Sister Mary Joseph nodule(SMJN)is an uncommon cutaneous metastasis found in the umbilicus,indicating an advanced *** typically originate from intra-abdominal sources,rarely from breast *** suggests a poor prognosis with a median survival of approximately 8 mo after *** patients with SMJNs is challenging,as most receive limited palliative care *** optimal strategy for long-term survival of these patients remains *** SUMMARY A 58-year-old female,previously diagnosed with right breast cancer 17 years ago and underwent breast-conserving surgery,adjuvant radiotherapy,and endocrine therapy,presented with a 2-cm umbilical *** years previously,metastases were detected in the right supraclavicular,infraclavicular,hilar,and mediastinal lymph *** umbilical nodule emerged four years before the date of presentation,confirmed as a skin metastasis of primary breast cancer upon excisional *** initial removal,the nodule recurred and grew,leading to her referral to our *** patient underwent extensive excision of the umbilical tumor and immediate abdominal wall *** therapy was continued *** years later,no local recurrence was observed,and the patient continued to work full-time,achieving over 9 years of survival following SMJN *** This case study aimed to identify the optimal strategy for achieving extended survival outcomes in patients with SMJN through comprehensive *** presented a case of the longest survival in a patient after undergoing a multidisciplinary treatment *** findings underscore the significance of adopting a multimodal treatment approach comprising timely and wide excision along with adjunctive *** approach can control the disease,prolong survival,and improve the quality of life in patients with SMJN.

关键词： Sister Mary Joseph nodule Breast cancer Cutaneous metastasis Long-term survival Abdominal wall reconstruction Case report

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