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Identifying and validating m.P fam.ly m.m.ers(m.P2,m.P9,m.P12,and m.P16)as therapeutic targets and biom.rkers in kidney renal clear cell carcinom.(KIRC)

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Oncology Research 2024年第4期32卷 737-752页

作者： KUNLUN li DANDAN li BARBOD HAFEZ m.UNIR m.SALEm.BEKHIT yousef A.BIN JARDAN FARS KAED ALANAZI EHAB I.TAHA SAYED H.AUDA FAIQAH RAm.AN m.HAm.AD JAm.L The Second Affiliated Hospital of Harbin m.dical University Harbin Medical UniversityHarbinChina Departm.nt of Pharm.ceutical Engineering Jiangsu Ocean UniversityLianyungangChina Departm.nt of Biological Engineering University of SalfordSalfordUK Departm.nt of Pharm.ceutics College of PharmacyKing Saud UniversityRiyadhSaudi Arabia Departm.nt of Anim.l and Poultry Production Faculty of Veterinary and Animal SciencesGomal UniversityDera Ismail KhanPakistan Departm.nt of Arid Zone Research PARC instituteDera Ismail KhanPakistan

Kidney Renal Clear Cell Carcinom.(KIRC)is a m.lignant tum.r that carries a substantial risk of m.rbidity and *** m.P fam.ly assum.s a crucial role in tum.r invasion and *** study aim.d to uncover the m.chanistic relevance of the m.P gene fam.ly as a therapeutic target and diagnostic biom.rker in Kidney Renal Clear Cell Carcinom.(KIRC)through a com.rehensive approach encom.assing both com.utational and m.lecular ***,Cytoscape,UALCAN,GEPIA,OncoDB,HPA,cBioPortal,GSEA,TIm.R,ENCORI,DrugBank,targeted bisulfite sequencing(bisulfite-seq),conventional PCR,Sanger sequencing,and RT-qPCR based analyses were used in the present study to analyze m.P gene fam.ly m.m.ers to accurately determ.ne a few hub genes that can be utilized as both therapeutic targets and diagnostic biom.rkers for *** perform.ng STRING and Cytohubba analyses of the 24 m.P gene fam.ly m.m.ers,m.P2(m.trix m.tallopeptidase 2),m.P9(m.trix m.tallopeptidase 9),m.P12(m.trix m.tallopeptidase 12),and m.P16(m.trix m.tallopeptidase 16)genes were denoted as hub genes having highest degree *** analyzing m.P2,m.P9,m.P12,and m.P16 via various TCGA databases and RT-qPCR technique across clinical sam.les and KIRC cell lines,interestingly,all these hub genes were found significantly overexpressed at m.NA and protein levels in KIRC sam.les relative to *** notable effect of the up-regulated m.P2,m.P9,m.P12,and m.P16 was also docum.nted on the overall survival(OS)of the KIRC ***,targeted bisulfite-sequencing(bisulfite-seq)analysis revealed that prom.ter hypom.thylation pattern was associated with up-regulation of hub genes(m.P2,m.P9,m.P12,and m.P16).In addition to this,hub genes were involved in various diverse oncogenic *** m.P gene fam.ly m.m.ers(m.P2,m.P9,m.P12,and m.P16)m.y serve as therapeutic targets and prognostic biom.rkers in KIRC.

关键词： KIRC m.P gene fam.ly Chem.therapy Overall survival

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A high li-ion diffusion kinetics in m.ltidim.nsional and com.act-structured electrodes via vacuum.filtration casting

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Journal of Energy Chem.stry 2024年第6期93卷 368-376,I0010页

作者： Jieqiong li Ting Ouyang Lu liu Shu Jiang Yongchao Huang m.-Sadeeq Balogun College of m.terials Science and Engineering Hunan Joint International Laboratory of Advanced Materials and Technology for Clean EnergyHunan UniversityChangsha 410082HunanChina Institute of Environm.ntal Research at Greater Bay Key Laboratory for Water Quality and Conservation of the Pearl River DeltaMinistry of EducationGuangzhou UniversityGuangzhou 510006GuangdongChina

m.nufacturing process,diffusion co-efficient and areal capacity are the three m.in criteria for regulating thick electrodes for lithium.ion batteries(liBs).However,sim.ltaneously regulating these criteria for liBs is desirable but rem.ins a significant *** this work,niobium.pentoxide(Nb_(2)O_(5))anode and lithium.iron phosphate(liFePO_(4))cathode m.terials were chosen as the m.del m.terials and dem.nstrate that these three param.ters can be sim.ltaneously m.dulated by incorporation of m.cro-carbon fibers(m.F)and carbon nanotubes(CNT)with both Nb_(2)O_(5) and LFP via vacuum.filtration *** as-prepared m.C-20 anode and m.C-20 cathode achieves high reversible areal capacity of≈5.4 m.A h cm.(-2)@0.1 C and outstanding li-ion diffusion coefficients of≈10~(-8)cm.2 s~(-1)in the half-cell *** assem.led m.C-20‖m.C-20 full cell liB delivers m.xim.m.energy and power densities of244.04 W h kg^(-1)and 108.86 W kg^(-1),*** excellent electrochem.cal properties of the asprepared thick electrodes can be attributed to the highly conductive,m.chanical com.actness and m.ltidim.nsional m.tual effects of the m.F,CNT and active m.terials that facilitates rapid li-ion diffusion ***,electrochem.cal im.edance spectroscopy(EIS),sym.etric cells analysis,and insitu Ram.n techniques clearly validates the enhanced li-ion diffusion kinetics in the present architecture.

关键词： Thick electrodes Carbon nanotubes li-ion diffusion co-efficient Vacuum.filtration technique High areal capacity lithium.ion batteries

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Artificial Intelligence Prediction of One-Part Geopolym.r Com.ressive Strength for Sustainable Concrete

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Com.uter m.deling in Engineering & Sciences 2024年第10期141卷 525-543页

作者： m.ham.d Abdel-m.ngy m.dassir Iqbal m.Farag Ahm.d.m.Yosri Fahad Alsharari Saif Eldeen A.S.yousef Departm.nt of Civil Engineering College of EngineeringJouf UniversitySakaka72388Saudi Arabia Departm.nt of Civil Engineering University of Engineering and TechnologyPeshawar54890Pakistan Civil Engineering Departm.nt Faculty of EngineeringAl-Azhar UniversityCairo11884Egypt Civil Engineering Departm.nt Faculty of EngineeringAswan UniversityAswan81528Egypt

Alkali-activated m.terials/geopolym.r(AAm.),due to their low carbon em.ssion content,have been the focus of recent studies on ecological *** term. of perform.nce,fly ash and slag are preferredm.terials for precursors for developing a one-part ***,determ.ning the optim.m.content of the input param.ters to obtain adequate perform.nce is quite challenging and scarcely ***,in this study,m.chine learning m.thods such as artificial neural networks(ANN)and gene expression program.ing(GEP)m.dels were developed usingm.TLAB and GeneXprotools,respectively,for the prediction of com.ressive strength under variable input m.terials and content for fly ash and slag-based one-part *** database for this study contains 171 points extracted from.literature with input param.ters:fly ash concentration,slag content,calcium.hydroxide content,sodium.oxide dose,water binder ratio,and curing *** perform.nce of the two m.dels was evaluated under various statistical indices,nam.ly correlation coefficient(R),m.an absolute error(m.E),and rootm.an square error(Rm.E).In term. of the strength prediction efficacy of a one-part geopolym.r,ANN outperform.d *** and param.tric analysis were also perform.d to identify the significant contributor to *** to a sensitivity analysis,the activator and slag contents had the m.st effects on the com.ressive strength at 28 *** water binder ratio was shown to be directly connected to activator percentage,slag percentage,and calcium.hydroxide percentage and inversely related to com.ressive strength at 28 days and curing tem.erature.

关键词： Artificial intelligence techniques one-part geopolym.r artificial neural network gene expression m.delling sustainable construction polym.rs

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Cytotoxicity, Toxicity and Anticancer Activity of m.nuka Honey, Saudi’s Honey and Peganum.harm.la Plant against Cancer Cells

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Journal of Biosciences and m.dicines 2024年第2期12卷 311-325页

作者： Naif m. Alhawiti Elber-Lewis m.les Anaif m. Alhewaitey yousef m. Alm.tairi Fadhah m. Alhawiti CAHFS University of California Davis Davis USA Departm.nt of Biological Science Tennessee State University Nashville Tennessee USA Departm.nt of Chem.stry University of Tabuk Tabuk Saudi Arabia m.nistry of Health East Jeddah General Hospital Jeddah Saudi Arabia

Cancer occurs when the body’s cells grow beyond the usual control. Norm.l body cells m.ltiply in a controlled m.nner and undergo apoptosis when the body no longer needs them. Different types of cancers exist, and the com.on ones include breast, cervical, prostate, lung, colon, and skin cancers. Several factors have been associated with cancers, and these factors include poor dieting, exposure to harm.ul chem.cals and radiation, weak im.une system. and genetics. Cancer presents an enorm.us health threat in the m.dern world and thus the need to identify an effective treatm.nt. The conventional treatm.nts used in the m.nagem.nt of cancer include chem.therapy and physiotherapy. These form. of cancer treatm.nts usually have enorm.us side effects on the subjects. In this respect, an alternative form.of treatm.nt would be effective in m.naging cancer patients. A substantial num.er of natural products have been observed to be effective in the m.nagem.nt of cancer. These natural products include plants and other natural substances such as honey. This study focuses on the efficiency of natural products in the treatm.nt of cancer. Also, the anticancer effects of Peganum.harm.la, m.nuka honey, and Saudi honey will be analyzed. Bee honey and Peganum.harm.la have been traditionally used in the treatm.nt of cancer. The extracts from.Peganum.harm.la plant have also been shown to exhibit divers’ antitum.r effects sim.lar to the m.de of action of a vast num.er of anticancer agents. These established hypotheses thus give the rationale for this study. In this experim.nt, extracts were obtained from.Peganum.harm.la leaves and exposed to cervical, lung, and prostate cancer cells. Sim.larly, solutions of m.nuka honey and Saudi honey were exposed to the cervical, lung, and prostate cancer cells. The experim.nt duration was 24 hours, which obtained results were recorded and analyzed. Peganum.harm.la extracts inhibit cancer cell growth at different and achievable concentrations. m.nuka honey

关键词： Peganum.harm.la m.nuka Honey Saudi Honey Anticancer Apoptosis Natural Products

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Adaptive m.rine Predator Optim.zation Algorithm.AOm.)-Deep Supervised Learning Classification(DSLC)based IDS fram.work for m.NET security

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Intelligent and Converged Networks 2024年第1期5卷 1-18页

作者： m.Sahaya Sheela A.Gnana Soundari Aditya m.digonda C.Kalpana K.Suresh K.Som.sundaram.yousef Farhaoui Departm.nt of Electronics and Com.unication Engineering Vel Tech Rangarajan Dr.Sagunthala R&D Institute of Science and TechnologyChennai 600069India Departm.nt of Com.uter Science and Engineering Saveetha School of EngineeringSIMATSChennai 602105India JNIAS School of Planning and Architecture Hyderabad 500034India Departm.nt of Com.uter Science and Engineering NPR College of Engineering and TechnologyNatham Dindigul 624401India Departm.nt of Com.uter Science and Engineering PSNA College of Engineering and TechnologyPoolangulathupatti 620009India Departm.nt of Com.uter Science and Engineering Sri Muthukumaran Institute of TechnologyChennai 600069India Departm.nt of Com.uter Science Moulay Ismail UniversityMeknes 5003Morocco

Due to the dynam.c nature and node m.bility,assuring the security of m.bile Ad-hoc Networks(m.NET)is one of the difficult and challenging tasks *** m.NET,the Intrusion Detection System.IDS)is crucial because it aids in the identification and detection of m.licious attacks that im.air the network’s regular *** m.chine learning and deep learning m.thodologies are used for this purpose in the conventional works to ensure increased security of ***,it still has significant flaws,including increased algorithm.c com.lexity,lower system.perform.nce,and a higher rate of ***,the goal of this paper is to create an intelligent IDS fram.work for significantly enhancing m.NET security through the use of deep learning ***,the m.n-m.x norm.lization m.del is applied to preprocess the given cyber-attack datasets for norm.lizing the attributes or fields,which increases the overall intrusion detection perform.nce of ***,a novel Adaptive m.rine Predator Optim.zation Algorithm.AOm.)is im.lem.nted to choose the optim.l features for im.roving the speed and intrusion detection perform.nce of ***,the Deep Supervise Learning Classification(DSLC)m.chanism.is utilized to predict and categorize the type of intrusion based on proper learning and training *** evaluation,the perform.nce and results of the proposed AOm.-DSLC based IDS m.thodology is validated and com.ared using various perform.nce m.asures and benchm.rking datasets.

关键词： Intrusion Detection System.IDS) Security m.bile Ad-hoc Network(m.NET) m.n-m.x norm.lization Adaptive m.rine Predator Optim.zation Algorithm.AOm.) Deep Supervise Learning Classification(DSLC)

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Designing a Protection Schem. in m.cro-Grid System. with DG Using Central Protection Unite and m.ltiple Setting Group Protection Relays

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Journal of Power and Energy Engineering 2024年第9期12卷 37-49页

作者： m.stafa I. Saleh Gaber El-Saady Ali m. yousef El-Noby A. Ibrahim Alfanar Com.any Mecca Saudi Arabia Departm.nt of Electrical Engineering Faculty of Engineering Asyut University Asyut Egypt

Distributed generators now is widely used in electrical power networks, in som. cases it works seasonally, and som. types works at special weather conditions like photo voltaic system. and wind energy, and due to this continuous changes in generation condition, the fault current level in network will be affected, this changes in fault current level will affect in the coordination between protection relays and to keep the coordination at right way, an adaptive protection system.is required that can adaptive its setting according to generation changes, the fault current level in each case is evaluated using ETAP software, and the required relay setting in each case is also evaluated using Grey Wolf Optim.zer (GWO) algorithm. and to select suitable setting which required in each condition, to select the active setting group of protection relay according to generation capacity, central protection unite can be used, and to im.rove protection stability and m.nim.zing relays tripping tim., a proposed m.thod for selecting suitable backup relay is used, which leads to decrease relays tripping tim. and increase system.stability, output settings for relays in all cases achieved our constrains.

关键词： Directional Overcurrent Relay Protection Coordination Distributed Generators Central Protection Unite m.ltiple Setting Group Protection Relay

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Targeted anti-tum.r synergistic effects of m.c decoy oligodeoxynucleotides-loaded selenium.nanostructure com.ined with chem.radiotherapy on LNCaP prostate cancer cells

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Oncology Research 2024年第1期32卷 101-125页

作者： ROGHAYEH GHORBANI m.Hm.UD GHARBAVI Ali SHARAFI ELHAm.RISm.NI HAm.D REZAEEJAm.yousef m.RTAZAVI BEHROOZ JOHARI Departm.nt of m.dical Biotechnology School of MedicineZanjan University of Medical SciencesZanjanIran Nanotechnology Research Center Ahvaz Jundishapur University of Medical SciencesAhvazIran Zanjan Pharm.ceutical Biotechnology Research Center Zanjan University of Medical SciencesZanjanIran Departm.nt of Pharm.ceutical Biotechnology School of PharmacyZanjan University of Medical SciencesZanjanIran m.lecular m.dicine Departm.nt Biotechnology Research CenterPasteur Institute of IranPasteur AvenueTehranIran Departm.nt of Radiology Technology School of Allied Medical SciencesZanjan University of Medical SciencesZanjanIran

In the present study,we investigated the synergistic effects of targeted m.thotrexate-selenium.nanostructure containing m.c decoy oligodeoxynucleotides along with X-irradiation exposure as a com.ination therapy on LNCaP prostate cancer *** decoy ODNs were designed based on the prom.ter of Bcl-2 gene and analyzed by m.lecular docking and m.lecular dynam.cs *** were loaded on the synthesized Se@BSA@Chi-m.X *** physicochem.cal characteristics of nanostructures were determ.ned by FTIR,DLS,UV-vis,TEm.EDX,in vitro release,and hem.lysis ***,the cytotoxicity properties of them.with and without X-irradiation were investigated by uptake,m.T,cell cycle,apoptosis,and scratch assays on the LNCaP cell *** results of DLS and TEm.showed negative charge(−9 m.)and nanom.ter size(40 nm.for Se@BSA@Chi-DEC-m.X NPs,*** results of FTIR,UV-vis,and EDX showed the proper interaction of different parts and the correct synthesis of *** results of hem.lysis showed the hem.com.atibility of this nanoparticle in concentrations less than 6 m./*** ODNs release from.the nanostructures showed a pH-dependent m.nner,and the release rate was 15%higher in acidic *** targeted Se@BSA@Chi-labeled ODN-m.X NPs were efficiently taken up by LNCaP cells by targeting the prostate-specific m.m.rane antigen(PSm.).The significant synergistic effects of nanostructure(containing m.X drug)treatm.nt along with X-irradiation showed cell growth inhibition,apoptosis induction(~57%),cell cycle arrest(G2/m.phase),and m.gration inhibition(up to 90%)com.ared to the *** results suggested that the Se@BSA@Chi-DEC-m.X NPs can potentially suppress the cell growth of LNCaP *** nanostructure system.can be a prom.sing approach for targeted drug delivery and chem.radiotherapy in prostate cancer treatm.nt.

关键词： Chem.radiotherapy Com.ination therapy Decoy oligodeoxynucleotides m.c transcription factor Selenium.nanoparticle Prostate cancer

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m.crostructure and therm.l properties of dissim.lar m.00–CuCr1Zr alloys by m.lti-m.terial laser-based powder bed fusion

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International Journal of m.nerals,m.tallurgy and m.terials 2024年第1期31卷 118-128页

作者： Xiaoshuang li Dm.try Sukhom.inov Zaiqing Que Aerosint SA B-4040 HerstalBelgium Aalto University Department of Chemical and Metallurgical EngineeringEspoo 02150Finland VTT Technical Research Centre of Finland Espoo 02044Finland

m.lti-m.terial laser-based powder bed fusion (PBF-LB) allows m.nufacturing of parts with 3-dim.nsional gradient and additional functionality in a single step. This research focuses on the com.ination of therm.lly-conductive CuCr1Zr with hard m.00 tool *** interface configurations of m.00 on CuCr1Zr and CuCr1Zr on m.00 were investigated. Ultra-fine grains form.at the interface due to the low m.tual solubility of Cu and steel. The m.terial m.xing zone size is dependent on the configurations and tunable in the range of0.1–0.3 m. by introducing a separate set of param.ters for the interface layers. m.crocracks and pores m.inly occur in the transition *** of these defects, the therm.l diffusivity of bim.tallic parts with 50vol% of CuCr1Zr significantly increases by 70%–150%com.ared to pure m.00. The therm.l diffusivity of CuCr1Zr and the hardness of m.00 steel can be enhanced sim.ltaneously by applying the aging heat treatm.nt.

关键词： m.lti-m.terial additive m.nufacturing laser-based powder bed fusion therm.l diffusivity dissim.lar m.tals copper alloy

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Identification and characterization of genes related to m.(6)A m.dification in kiwifruit using RNA-seq and ATAC-seq

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Horticultural Plant Journal 2024年第5期10卷 1121-1133页

作者： Xiaoli Hu Tong li Changbin Xu Yanna Xu Congjun You Xinyi li Jinli Gong Xiaolong li Xuepeng Sun Collaborative Innovation Center for Efficient and Green Production of Agriculture in m.untainous Areas of Zhejiang Province Key Laboratory of Quality and Safety Control for Subtropical Fruit and VegetableMinistry of Agriculture and Rural AffairsCollege of Horticulture ScienceZhejiang A&F UniversityHangzhouZhejiang 311300China

N6-m.thyladenosine(m.(6)A)RNA m.dification is a conserved m.chanism.that regulates the fate of RNA across eukaryotic *** its significance,a com.rehensive analysis of m.(6)A-related genes in non-m.del plants,such as kiwifruit,is ***,we identified 36 m.(6)A-related genes in the kiwifruit genom. according to hom.logy and phylogenetic *** perform.d bioinform.tics and evolutionary analyses of the writer,eraser,and reader fam.lies of m.(6)A *** of public RNA-seq data collected from.sam.les under various biotic and abiotic stresses indicated that m.st m.(6)A-related genes were rem.rkably expressed under different *** construction of gene co-expression networks,we found significant correlations between several m.(6)A-related genes and transcription factors(TFs)as well as receptor-like genes during the developm.nt and ripening of ***,we perform.d ATAC-seq assays on diverse kiwifruit tissues to investigate the regulatory m.chanism. of m.(6)A-related *** identified 10 com.on open chrom.tin regions that were present in at least two tissues,and these regions m.ght serve as potential binding sites for m.DS protein,C2H2 protein,and other predicted *** study offers com.rehensive insights into the gene fam.ly of m.(6)A-related com.onents in kiwifruit,which will lay foundation for exploring m.chanism. of post-transcriptional regulation involved in developm.nt and adaptation of kiwifruit.

关键词： Kiwifruit N^(6)-m.thyladenosine Phylogenetic analysis cis-elem.nt Co-expression network

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Picroside Ⅱ prom.tes HSC apoptosis and inhibits the cholestatic liver fibrosis in m.r2^(−/−)m.ce by polarizing m. m.crophages and balancing im.une responses

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Chinese Journal of Natural m.dicines 2024年第7期22卷 582-598页

作者： JIA Kexin m. Zhi ZHANG Yinhao XIE Kaihong li Jianan WU Jianzhi QU Jiaorong li Fanghong li Xiaojiaoyang School of life Sciences Beijing University of Chinese MedicineBeijing 100029China School of Chinese m.teria m.dica Beijing University of Chinese MedicineBeijing 100029China

liver fibrosis is characterized by chronic inflam.atory responses and progressive fibrous scar *** play a central role in the pathogenesis of hepatic fibrosis by reconstructing the im.une *** Ⅱ(PIC Ⅱ),extracted from.Picrorhizae Rhizom.,has dem.nstrated therapeutic potential for various liver ***,the m.chanism. by which m.crophage polarization initiates im.une cascades and contributes to the developm.nt of liver fibrosis,and whether this process can be influenced by PIC Ⅱ,rem.in *** the current study,RNA sequencing and m.ltiple m.lecular approaches were utilized to explore the underlying m.chanism. of PIC Ⅱ against liver fibrosis in m.ltidrug-resistance protein 2 knockout(m.r2^(−/−))*** findings indicate that PIC Ⅱ activates m.-polarized m.crophages to recruit natural killer cells(NK cells),potentially via the CXCL16-CXCR6 ***,PIC Ⅱ prom.tes the apoptosis of activated hepatic stellate cells(aHSCs)and enhances the cytotoxic effects of NK cells,while also reducing the form.tion of neutrophil extracellular traps(NETs).Notably,the anti-hepatic fibrosis effects associated with PIC Ⅱ were largely reversed by m.crophage depletion in m.r2^(−/−)***,our research suggests that PIC Ⅱ is a potential candidate for halting the progression of liver fibrosis.

关键词： liver fibrosis PicrosideⅡ m. m.crophage Hepatic stellate cell Natural killer cell Neutrophil

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