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Roles of miR319-regulated TCPs in plant development and response to abiotic stress

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The Crop Journal 2021年第1期9卷 17-28页

作者： Yujie Fang Yuqian Zheng Wei Lu Jian Li Yujing Duan Shuai Zhang youping wang Key Laboratory of Plant Functional Genomics of the Ministry of Education Jiangsu Key Laboratory of Crop Genomics and Molecular BreedingYangzhou UniversityYangzhou 225009JiangsuChina Joint International Research Laboratory of Agriculture and Agri-Product Safety the Ministry of Education of ChinaYangzhou UniversityYangzhou 225009JiangsuChina

Elaborate regulation of gene expression is required for plants to maintain normal growth,development,and ***(miRNAs)and transcription factors are key players that control gene expression in plant regulatory *** TEOSINTE BRANCHED1/CYCLOIDEA/PROLIFERATING CELL FACTOR(TCP)family comprises plantspecific transcription factors that contain a conserved TCP domain of 59 amino *** members of this family are targeted by miR319,one of the most ancient and evolutionarily conserved miRNAs in *** evidence has revealed that miR319-regulated TCP(MRTCP)genes participate extensively in plant development and responses to environmental *** this review,the structural characteristics and classifications of TCP transcription factors and the regulatory relationships between TCP transcription factors and miRNAs are *** knowledge of the regulatory functions of MRTCP genes in multiple biological pathways including leaf development,vascular formation,flowering,hormone signaling,and response to environmental stresses such as cold,salt,and drought is *** review will be beneficial for understanding the roles of the MRTCP-mediated regulatory network and its molecular mechanisms in plant development and stress response,and provides a theoretical basis for plant genetic improvement.

关键词： TCP miR319 Regulation Growth and development Abiotic stress

Slope-climbing of cropland and its effects in China

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Journal of Mountain Science 2024年第8期21卷 2754-2769页

作者： CHEN Wanxu XIE youping YUAN Jintao ZENG Jie YANG Liyan GU Tianci LEI Fan Hubei Key Laboratory of Regional Ecology and Environmental Change School of Geography and Information EngineeringChina University of GeosciencesWuhan 430074China Key Laboratory of Natural Resources Monitoring and Supervision in Southern Hilly Region Ministry of Natural ResourcesChangsha 410118China Yunnan Key Laboratory of Plateau Geographic Processes and Environmental Changes Faculty of GeographyYunnan Normal UniversityKunming 650500China

In recent decades,the spatio-temporal patterns of China’s croplands have been reshaped by disturbances from anthropogenic activities,with complex changes in the topographic characteristics of ***-climbing of croplands(SCCL)is an important issue that threatens sustainable agricultural *** providing land with prominent location advantages,SCCL weakens the water and fertilizer retention capacity for cropland,intensifies various geological disasters,and adversely affects the ecological environment and food yield of these *** is crucial to determine the spatio-temporal variation features and effects of SCCL in China to formulate more accurate cropland protection policies and to maintain food security;however,the current lack of relevant studies is detrimental for capturing trends in cropland resources and sustainable cropland *** this study,we constructed a multi-scale slope spectrum for cropland and total terrain to explore the spatial differences and trends of SCCL from a three-dimensional *** evaluated the natural and socioeconomic effects of SCCL in China from multiple *** indicate that the proportion of cropland with slopes below 2°,5°,and 6°in China decreased by 0.43%,0.47%,and 0.50%from 1980 to 2020,*** became apparent during 1980-1990 and 2010-2020,especially over the recent *** cropland climbing index(CCI)and upper limited slope change(ULSC)to measure the spatio-temporal pattern of SCCL were 0.99%and 1.17°,respectively,during *** the agricultural regional scale,the SCCL was also concentrated in 1980-1990 and 2010-2020,and it is more pronounced in the southern *** proportion of provinces and prefecture-level cities with high-intensity SCCL during 1980-2020 were 87.10%and 49.73%,*** was comparatively more pronounced and broader from 2010 to *** this period,17.84%of prefecture-level cities had no SCCL,and the average CCI for all prefecture-level citi

关键词： Croplands Slope spectrum Slope-climbing Three-dimensional perspective Multi-scale China

Comparative proteomics analysis of OsNAS1 transgenic Brassica napus under salt stress

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Chinese Science Bulletin 2011年第22期56卷 2343-2350页

作者： KONG Fang MAO ShanJing DU Kun WU Mian ZHOU XiaoYan CHU ChengCai wang youping College ofBioscience andBiotechnology Yangzhou University Yangzhou 225009 China Academy of Biological and Chemical Engineering Anhui Polytechnic University Wuhu 241000 China Institute of Genetics and Developmental Biology Chinese Academy of Sciences Beijing 100101 China

Salt stress is one of the major abiotic stresses in agricultural plants *** used proteomics to analyze the differential expression of proteins in transgenic OsNAS1 and non-transformant Brassica napus treated with 20 mmol/L *** protein from the leaves was extracted and separated through a high-resolution and highly repetitive two-dimensional electrophoresis(2-DE)technology *** protein spots were reproducibly observed to be upregulated by more than 2-fold between transgenic and non-transformant *** 12 spots were digested in-gel with trypsin and characterized by matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry(MALDI-TOF-MS)to obtain the peptide mass *** database searching revealed that 5 of these proteins are involved in salt tolerance:dehydrogenase,glutathione S-transferase,peroxidase,20S proteasome beta subunit,and ribulose-1,5-bisphosphate carboxylase/*** potential functions of these identified proteins in substance and energy metabolism,stress tolerance,protein degradation,and cell defense are *** salt tolerance of the transgenic rapeseed was significantly improved by the introduction of the OsNAS1 gene from Brazilian upland rice of Oryza sativa(*** 9).

关键词：比较蛋白质组转基因油菜甘蓝型油菜盐胁迫学分飞行时间质谱二磷酸羧化酶谷胱苷肽

Co-location of QTL for Sclerotinia stem rot resistance and flowering time in Brassica napus

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The Crop Journal 2019年第2期7卷 227-237页

作者： Jian Wu Peipei Chen Qing Zhao Guangqin Cai Yue Hu Yang Xiang Qingyong Yang youping wang Yongming Zhou Jiangsu Provincial Key Laboratory of Crop Genetics and Physiology Yangzhou University National Key Laboratory of Crop Genetic Improvement Huazhong Agricultural University Agricultural Bioinformatics Key Laboratory of Hubei Province College of Informatics Huazhong Agricultural University Guizhou Rapeseed Institute Guizhou Academy of Agricultural Sciences

Sclerotinia stem rot(SSR) caused by Sclerotinia sclerotiorum(Lib.) de Bary is one of the most devastating diseases of Brassica napus worldwide. Both SSR resistance and flowering time(FT) adaptation are major breeding goals in B. napus. However, early maturing rapeseed varieties, which are important for rice-rapeseed rotation in China, are often highly susceptible to SSR. Here, we found that SSR resistance was significantly negatively correlated with FT in a natural population containing 521 rapeseed inbred lines and a double haploid(DH) population with 150 individual lines, both of which had great variation in FT. Four chromosomal regions on A2, A6, C2, and C8 affecting both SSR resistance and FT were identified using quantitative trait loci(QTL) mapping after constructing a high-density genetic map based on single nucleotide polymorphism markers in the DH ***, we aligned QTL for the two traits identified in the present and previous studies to the B. napus reference genome, and identified four colocalized QTL hotspots of SSR resistance and FT on A2(0–7.7 Mb), A3(0.8–7.5 Mb), C2(0–15.2 Mb), and C6(20.2–36.6 Mb). Our results revealed a genetic link between SSR resistance and FT in ***, which should facilitate the development of effective strategies in both early maturing and SSR resistance breeding and in map-based cloning of SSR resistance QTL.

关键词： Sclerotinia stem rot Brassica napus QTL mapping Flowering time SNP array

Expression Patterns of ABA and GA Metabolism Genes and Hormone Levels during Rice Seed Development and Imbibition: A Comparison of Dormant and Non-Dormant Rice Cultivars

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Journal of Genetics and Genomics 2014年第6期41卷 327-338页

作者： Yang Liu Jun Fang Fan Xu Jinfang Chu Cunyu Yan Michael R.Schlappi youping wang Chengcai Chu State Key Laboratory of Plant Genomics and National Center for Plant Gene Research (Beijing) Institute of Genetics and Developmental BiologyChinese Academy of Sciences National Center for Plant Gene Research (Beijing) Institute of Genetics and Developmental Biology Chinese Academy of Sciences Department of Biological Sciences Marquette University College of Life Sciences Yangzhou University

Seed dormancy is an important agronomic trait in cereals. Using deep dormant （N22）, medium dormant （ZH11）, and non-dormant （G46B） rice cultivars, we correlated seed dormancy phenotypes with abscisic acid （ABA） and gibberellin （GA） metabolism gene expression profiles and phytohormone levels during seed development and imbibition. A time course analysis of ABA and GA content during seed development showed that N22 had a high ABA level at early and middle seed developmental stages, while at late developmental stage it declined to the level of ZHll; however, its ABA/GA ratio maintained at a high level throughout seed development. By contrast, G46B had the lowest ABA content during seed development though at early developmental stage its ABA level was close to that of ZH11, and its ABA/GA ratio peaked at late developmental stage that was at the same level of ZHll. Compared with N22 and G46B, ZH11 had an even and medium ABA level during seed development and its ABA/GA ratio peaked at the middle developmental stage. Moreover, the seed development time-point having high ABA/GA ratio also had relatively high transcript levels for key genes in ABA and GA metabolism pathways across three cultivars. These indicated that the embryo-imposed dormancy has been induced before the late developmental stage and is determined by ABA/GA ratio. A similar analysis during seed imbibition showed that ABA was synthesized in different degrees for the three cultivars. In addition, water uptake assay for intact mature seeds suggested that water could permeate through husk barrier into seed embryo for all three cultivars; however, all three cultivars showed distinct colors by vanillin-staining indicative of the existence of flavans in their husks, which are dormancy inhibition compounds responsible for the husk-imposed dormancy.

关键词： Seed development Seed imbibition Gene expression Abscisic acid (ABA) Gibberellin (GA) ABA/GA ratio Rice

Assessment on alien herbicide-resistant gene flow among crucifers by sexual compatibility

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Chinese Science Bulletin 2005年第15期50卷 1604-1611页

作者： ZHAO Xiangxiang LU Weiping QI Cunkou PU Huiming XIA Qiuxia LU Dalei LIU Geshan wang youping Jiangsu Provincial Key Lab of Crop Genetics and Physiology Yangzhou University Yangzhou 225009 China Institute of Industrial Crops Jiangsu Academy of Agricultural Sciences Nanjing 210014 China College of Bioscience and Biotechnology Yangzhou UniversityYangzhou 225009 China

Cross-pollination and high cross-compatibility are frequently found in many cruciferous plants, which im-plies that there might be potential risk of alien gene flow among crucifers. In this work, the alien glyphosate-resistant gene flow between GM (Genetically modified) rapeseed vari-ety Q3 (Brassica napus) and 6 varieties from 5 cruciferous species was assessed by sexual compatibility through aniline blue fluorescence observation, manual cross, mentor pollina-tion with the mixture pollen and DNA identification. The results indicated that the compatibility indices of two B. napus varieties, B. juncea var. multiceps Tsen et Lee and Chinese cabbage (B. chinensis) (as female) with Q3 (as male) were 16.15, 12.77, 2.345 and 0.85, respectively. F1 plants were examined by PCR and all of these samples were confirmed as positive. Crossing between Orychophragmus violaceus, Des-curainia sophia (as female) and Q3 (as male) expressed high cross-incompatibility. Fluorescence microscope observation indicated that growing of pollen tube terminated at the upper 1/3 part of the style of O. violaceus and on the stigma surface of D. sophia. The mentor plants were further analyzed by PCR and all were shown to be negative. Under the neighboring growth and natural pollination conditions, the rates of gene flow to two B. napus varieties were 2.3278% and 2.1487%, respectively, B. juncea var. multiceps Tsen et Lee and Chinese cabbage were 1.0157% and 0.9236%, re-spectively. No gene flow was observed in O. violaceus and D. sophia. Frequency of gene flow was highly correlated with sexual compatibility. Due to possible alien gene flow among crucifers, special consideration and care should be taken to grow GM rapeseed.

关键词：除草剂十字花科植物荧光性遗传因子

A New Device for Gas-Liquid Flow Measurements Relying on Forced Annular Flow

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Fluid Dynamics & Materials Processing 2024年第8期20卷 1759-1772页

作者： Tiantian Yu youping Lv Hao Zhong Ming Liu Pingyuan Gai Zeju Jiang Peng Zhang Xingkai Zhang Petroleum Engineering Technology Research Institute SINOPEC Shengli Oilfield CompanyDongying257000China School of Petroleum Engineering Yangtze UniversityWuhan430100China

A new measurement device,consisting of swirling blades and capsule-shaped throttling elements,is proposed in this study to eliminate typical measurement errors caused by complex flow patterns in gas-liquid *** swirling blades are used to transform the complex flow pattern into a forced annular *** on the research of existing blockage flow meters and also exploiting the single-phase flow measurement theory,a formula is introduced to measure the phase-separated flow of gas and *** formula requires the pressure ratio,Lockhart-Martinelli number(L-M number),and the gas phase Froude *** unknown parameters appearing in the formula are fitted through numerical simulation using computational fluid dynamics(CFD),which involves a comprehensive analysis of the flow field inside the device from multiple perspectives,and takes into account the influence of pressure ***,the measurement model is validated through an experimental error *** results demonstrate that the measurement error can be maintained within±8%for various flow patterns,including stratified flow,bubble flow,and wave flow.

关键词： Gas-liquid flow measurement blocking flowmeter measurement model pressure fluctuations numerical simulation experimental control

Effects of Sinochem Microbial Organic Fertilizer on Tea Plants

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Agricultural Biotechnology 2022年第6期11卷 167-170,173页

作者： Xun CHEN Gaixiang LEI Danjuan HUANG Hongjuan wang Rongrong TAN Wei KANG youping wang Jiong WU Xian ZHANG Yingxin MAO Institute of Fruit and Tea Hubei Academy of Agriculture SciencesWuhan 430209China Chibi Agricultural and Rural Bureau Chibi 437300China Hubei Sinochem Orient Fertilizer Co. Ltd.Wuhan 430213China Yingshan Public Testing Center Huanggang 438700China Chibi Zhaoliqiao Tafeng Tea Co. Ltd.Chibi 437399China

[Objectives]This study was conducted to explore the application effects of Sinochem microbial organic fertilizer on tea plants.[Methods]Through the experiment and demonstration of Sinochem microbial organic fertilizer,its effects on the yield and quality of tea and the physical and chemical quality of soil were explored.[Results]Compared with the conventional organic fertilizer cake fertilizer,the fertilizer could increase the weight of freshly picked bud leaves,increase the yield of tea,and thus promote the growth of tea *** increased the contents of chlorophyll components,amino acids and tea polyphenols,the main functional substances of tea,which was conducive to improving the internal quality of *** improved the physical and chemical properties of soil,and increased the contents of organic matter,alkali-hydrolyzable nitrogen and available phosphorus in tea garden *** also had a good effect in improving soil environmental health indexes and physical and chemical properties,and did not contain heavy metals and plant growth hormones.[Conclusions]This study provides a theoretical basis for the popularization and application of Sinochem microbial organic fertilizer.

关键词： Tea plant Biological Organic fertilizer Application effect

A Preliminary Study on Guangdong Shangyuan Fire Dragon Dance Culture

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China & The World Cultural Exchange 2024年第4期 34-37页

作者： Huang youping Foshan Museum

Dragon is a traditional totem of the Chinese nation and is worshiped by the Chinese people all over the world,representing auspiciousness,strength and *** has a vast *** for the rich and diverse intangible cultural he... 详细信息

关键词： dragon has Dance