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Detection of Listeria monocytogenes in Foods and Characterization by PFGE

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Advances in Microbiology 2016年第4期6卷 343-349页

作者： Sanghun Park Hyowon Jung Myungsuk Lee Heejin Choi Jimin Kim Jihun Jung Sungkyu Park Musang Kim Kyungsik Kim younghee oh Aehee Chung Kweon Jung Seoul Metropolitan Government Research Institute of Public Health and Environment Gwacheon-si Korea

The aims of the present study were to investigate the prevalence of Listeria monocytogenes in 1042 foods collected from different market to characterize the isolates by phenotypical and molecular methods. In particular, L. monocytogenes obtained from different types of foods such as RTE (kimbap), fish (smoked salmon and seasoned-dried slice fish) and meat (cut raw beef and pork) from 2009 to 2011, were used. Twelve samples (2.1%) were positive for L. monocytogenes. Detection rate of L. monocytogenes varied significantly by food type and ranged from 1.1% to 5.2%. Meat is the highest prevalence for L. monocytogenes (5.2%) followed by RTE (1.8%) and Fish (1.1%). Twelve isolates were also serotyped by the agglutination method. The most common serotypes detected in the 12 strains tested were 1/4b (75.0%), followed by 1/2a (16.7%), and 1/2b (8.3%). For this study, we used serotyping and detected 6 different virulence-associated genes (inlA, inlB, plcA, plcB, hlyA, and actA) and 16s rRNA using multiplex-PCR. PFGE was performed to determine genetic characterization of L. monocytogenes strains to define the genetic diversity.

关键词： Listeria monocytogenes PFGE RTE

Human Rhinoviruses in Korean with Acute Low Respiratory Tract Infections

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Open Journal of Medical Microbiology 2015年第4期5卷 237-245页

作者： Sanghun Park Sungsun Choi Jungim Jang Eunjeung Kim Seokju Cho Jihun Jung Kyungsik Kim Sungkyu Park Musang Kim Yeonsun Kim younghee oh Kweon Jung Seoul Metropolitan Government Research Institute of Public Health and Environment Infectious Disease Research Division Gwacheon-si Korea Department of Public Health Graduate School Dankook University Seoul Korea

Human rhinovirus (HRV) must be viewed as a significant etiological agent of acute low respiratory tract infections (ALRIs) in infants and young children. The present study has been carried out to investigate the prevalence of recently identified respiratory viruses and determine the phylogenetic composition of HRV strains. In total, 923 nasopharyngeal aspirates (NPAs) collected from hospitalized patients with ALRIs between January and December 2010 (453 females and 470 males) at five referral hospitals in Seoul were tested for respiratory viruses. Viruses were detected by RT-PCR (Reverse transcription polymerase chain reaction) in 501 (54.3%) of the 923 positive samples obtained. Overall, HRV was detected in 7.9%, ADV in 10.2%, IFV in 21.9%, RSV in 3.6%, hMPV in 2.7%, HCoV in 2.5%, HEV in 1.6%, HBoV in 1.6%, and PIV in 2.4% of the samples. HRV infections occurred throughout the year, with peaks in January, May, July, and November of 2010. Phylogenetic analyses using the VP4/VP2 cording region showed that among the 27 isolates with HRV, 14 (52%) were infected with species A, 2 (7%) were infected with species B, and 11 (41%) were infected with the strains from HRV-C. HRV-C sequences are genetically distinct, sharing only 32% to 33% of their amino acids with HRV-A and HRV-B, while retaining 46% to 100% identity with each other. HRV-A, B, and C were co-circulating in children hospitalized with ALRIs in Korea in 2010. These findings indicated that HRV-C was the important causative agent of HRV associated with ALRIs.

关键词： NPAs ALRIs Human Rhinovirus

Comparative Analysis of Pathogenicity Assays and PCR for <i>Listeria monocytogenes</i>Isolated from Animal, Raw Food and Environmental Sources in Korea

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Advances in Microbiology 2013年第2期3卷 191-199页

作者： Sanghun Park Jihun Jung younghee oh Jibho Lee Seunghee Ryu Hyowon Jung Sunhee Park Miok Song Gunyong Park Sungmin Choi Sangmi Lee Junghun Kim YoungZoo Chae Byungyeol Jung Myunghun Lee Hyunsoo Kim Seoul Metropolitan Government Research Institute of Public Health and Environment Yangjae-Dong Seocho-Gu Seoul Korea

Results of PCR with oligonucleotide primers were designed from the assembled panel of four potential virulence genes (two of internalin gene and two of transcriptional regulator gene). Most of the isolates including reference strains were reactive by PCR, whereas the other strains (No.80, 81, and 83) isolated from pork, were non-reactive by PCR. In particular, all pork isolates were PCR-negative for two primers (lmo2672 and 2821) sets tested. However, No.82 was positive for lmo1134 primer, and No.84 was positive for lmo2470 of pork isolates. It was observed that all Listeria monocytogenes (L. monocytogenes) penetrate Vero cells, although the invasion efficiency of each strain varied (between 0.5 and 18.9%). When compared in cell assay with PFGE, the results were shown that the mean invasion efficiency for lineage II isolate (2.6%) was significantly lower (ANOVA-test,

关键词： PFGE Vero Cell Listeria monocytogenes in Vitro Assay

Molecular Characterization of <i>Listeria monocytogenes</i>Based on the PFGE and RAPD in Korea

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Advances in Microbiology 2012年第4期2卷 605-616页

作者： Sanghun Park Jihun Jung Sungsun Choi younghee oh Jibho Lee Heesun Chae Seunghee Ryu Hyowon Jung Gunyong Park Sungmin Choi Bogsoon Kim Junghun Kim Young Zoo Chae Byungyeol Jung Myunghun Lee Hyunsoo Kim Animal Disease Diagonsis Division National Veterinary Research and Quarantine Service Anyang City Korea College of Veterinary Medcine Chungnam National University Daejeon Korea Seoul Metropolitan Government Research Institute of Public Health and Environment Seoul Korea Veterinary Pharmacology Division National Veterinary Research and Quarantine Service Anyang City Korea

This study was performed to characterize 35 L. monocytogenes isolates from animals, foods, environmental samples collected between 1997 and 2007 with no apparent epidemiological relations, and five reference isolates using serotypic, genotypic and molecular typing methods to understand the pattern of strain distribution in Korea. For this study, we used serotyping and detected 6 different virulence-associated genes (inlA, inlB, plcA, plcB, hlyA, and actA) and 16s rRNA using multiplex-PCR. We also compared RAPD and PFGE to determine genetic characterization of L. monocytogenes strains to define the genetic diversity. Serotype patterns of the 30 L. monocytogenes strains was as follows;9 isolates (30.0%) belonged to serotype, 7 isolates (23.3%) belonged to serotype 4b, 4 isolates (13.3%) belonged to serotype 1/2b, 3 isolates (10.0%) belonged to serotype 1/2c, 2 (6.7%) isolates belonged to4c, 2 (6.7%) isolates belonged to NT (Non Type), one isolate (3.2%) belonged to3aand 3b, and4a, respectively. Although, a limited number of isolates were analyzed in this study, molecular typing with RAPD and PFGE indicated that PFGE is more discriminatory for the subtyping L. monocytogenes than RAPD. Some L. monocytogenes isolates by RAPD and PFGE types are associated with specific sources. And, combining data obtained by these methods will increases the likelihood of strain discrimination.

关键词： Listeria monocytogenes PFGE RAPD