检索结果-南通市图书馆

Detection of Listeria monocytogenes in Foods and Characterization by PFGE

Advances in Microbiology 2016年第4期6卷 343-349页

作者： Sanghun Park Hyowon Jung Myungsuk Lee Heejin Choi Jimin kim Jihun Jung Sungkyu Park Musang kim Kyungsik kim younghee Oh Aehee Chung Kweon Jung Seoul Metropolitan Government Research Institute of Public Health and Environment Gwacheon-si Korea

The aims of the present study were to investigate the prevalence of Listeria monocytogenes in 1042 foods collected from different market to characterize the isolates by phenotypical and molecular methods. In particular, L. monocytogenes obtained from different types of foods such as RTE (kimbap), fish (smoked salmon and seasoned-dried slice fish) and meat (cut raw beef and pork) from 2009 to 2011, were used. Twelve samples (2.1%) were positive for L. monocytogenes. Detection rate of L. monocytogenes varied significantly by food type and ranged from 1.1% to 5.2%. Meat is the highest prevalence for L. monocytogenes (5.2%) followed by RTE (1.8%) and Fish (1.1%). Twelve isolates were also serotyped by the agglutination method. The most common serotypes detected in the 12 strains tested were 1/4b (75.0%), followed by 1/2a (16.7%), and 1/2b (8.3%). For this study, we used serotyping and detected 6 different virulence-associated genes (inlA, inlB, plcA, plcB, hlyA, and actA) and 16s rRNA using multiplex-PCR. PFGE was performed to determine genetic characterization of L. monocytogenes strains to define the genetic diversity.

关键词： Listeria monocytogenes PFGE RTE

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Molecular Characterization of <i>Listeria monocytogenes</i>Based on the PFGE and RAPD in Korea

引用

Advances in Microbiology 2012年第4期2卷 605-616页

作者： Sanghun Park Jihun Jung Sungsun Choi younghee Oh Jibho Lee Heesun Chae Seunghee Ryu Hyowon Jung Gunyong Park Sungmin Choi Bogsoon kim Junghun kim Young Zoo Chae Byungyeol Jung Myunghun Lee Hyunsoo kim Animal Disease Diagonsis Division National Veterinary Research and Quarantine Service Anyang City Korea College of Veterinary Medcine Chungnam National University Daejeon Korea Seoul Metropolitan Government Research Institute of Public Health and Environment Seoul Korea Veterinary Pharmacology Division National Veterinary Research and Quarantine Service Anyang City Korea

This study was performed to characterize 35 L. monocytogenes isolates from animals, foods, environmental samples collected between 1997 and 2007 with no apparent epidemiological relations, and five reference isolates using serotypic, genotypic and molecular typing methods to understand the pattern of strain distribution in Korea. For this study, we used serotyping and detected 6 different virulence-associated genes (inlA, inlB, plcA, plcB, hlyA, and actA) and 16s rRNA using multiplex-PCR. We also compared RAPD and PFGE to determine genetic characterization of L. monocytogenes strains to define the genetic diversity. Serotype patterns of the 30 L. monocytogenes strains was as follows;9 isolates (30.0%) belonged to serotype, 7 isolates (23.3%) belonged to serotype 4b, 4 isolates (13.3%) belonged to serotype 1/2b, 3 isolates (10.0%) belonged to serotype 1/2c, 2 (6.7%) isolates belonged to4c, 2 (6.7%) isolates belonged to NT (Non Type), one isolate (3.2%) belonged to3aand 3b, and4a, respectively. Although, a limited number of isolates were analyzed in this study, molecular typing with RAPD and PFGE indicated that PFGE is more discriminatory for the subtyping L. monocytogenes than RAPD. Some L. monocytogenes isolates by RAPD and PFGE types are associated with specific sources. And, combining data obtained by these methods will increases the likelihood of strain discrimination.

关键词： Listeria monocytogenes PFGE RAPD

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Comparative Analysis of Pathogenicity Assays and PCR for <i>Listeria monocytogenes</i>Isolated from Animal, Raw Food and Environmental Sources in Korea

引用

Advances in Microbiology 2013年第2期3卷 191-199页

作者： Sanghun Park Jihun Jung younghee Oh Jibho Lee Seunghee Ryu Hyowon Jung Sunhee Park Miok Song Gunyong Park Sungmin Choi Sangmi Lee Junghun kim YoungZoo Chae Byungyeol Jung Myunghun Lee Hyunsoo kim Seoul Metropolitan Government Research Institute of Public Health and Environment Yangjae-Dong Seocho-Gu Seoul Korea

Results of PCR with oligonucleotide primers were designed from the assembled panel of four potential virulence genes (two of internalin gene and two of transcriptional regulator gene). Most of the isolates including reference strains were reactive by PCR, whereas the other strains (No.80, 81, and 83) isolated from pork, were non-reactive by PCR. In particular, all pork isolates were PCR-negative for two primers (lmo2672 and 2821) sets tested. However, No.82 was positive for lmo1134 primer, and No.84 was positive for lmo2470 of pork isolates. It was observed that all Listeria monocytogenes (L. monocytogenes) penetrate Vero cells, although the invasion efficiency of each strain varied (between 0.5 and 18.9%). When compared in cell assay with PFGE, the results were shown that the mean invasion efficiency for lineage II isolate (2.6%) was significantly lower (ANOVA-test,

关键词： PFGE Vero Cell Listeria monocytogenes in Vitro Assay

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：